EBI Dbfetch

ID   AB078775; SV 1; linear; genomic DNA; STD; PRO; 1859 BP.
XX
AC   AB078775;
XX
DT   24-JUL-2002 (Rel. 72, Created)
DT   14-APR-2005 (Rel. 83, Last updated, Version 2)
XX
DE   Bacillus circulans bglM gene for 1,3-(1,3;1,4)-beta-D-glucan
DE   3(4)-glucanohydrolase, complete cds.
XX
KW   .
XX
OS   Bacillus circulans
OC   Bacteria; Firmicutes; Bacillales; Bacillaceae; Bacillus.
XX
RN   [1]
RP   1-1859
RA   Aono R., Asano T.;
RT   ;
RL   Submitted (23-JAN-2002) to the EMBL/GenBank/DDBJ databases.
RL   Rikizo Aono, Tokyo Institute of Technology, Department of Biological
RL   Information; Midori-ku, Nagatsuta, 4259, Yokohama, Kanagawa 226-8501, Japan
RL   (E-mail:raono@bio.titech.ac.jp, Tel:81-45-924-5766, Fax:81-45-924-5819)
XX
RN   [2]
RX   DOI; 10.1271/bbb.66.1246
RX   PUBMED; 12162545.
RA   Asano T., Taki J., Yamamoto M., Aono R.;
RT   "Cloning and structural analysis of bglM gene coding for the fungal cell
RT   wall-lytic beta-1,3-glucan-hydrolase BglM of Bacillus circulans IAM1165";
RL   Biosci. Biotechnol. Biochem. 66(6):1246-1255(2002).
XX
FH   Key             Location/Qualifiers
FH
FT   source          1..1859
FT                   /organism="Bacillus circulans"
FT                   /mol_type="genomic DNA"
FT                   /db_xref="taxon:1397"
FT   RBS             79..84
FT   CDS             89..1324
FT                   /codon_start=1
FT                   /transl_table=11
FT                   /gene="bglM"
FT                   /product="1,3-(1,3;1,4)-beta-D-glucan
FT                   3(4)-glucanohydrolase"
FT                   /note="Enzyme BglM (42 kDa) degrades Aspergillus oryzae
FT                   cell walls actively. The central domain of enzyme BglM
FT                   shows a bacterial beta-1,3-glucan-hydrolase motif belonging
FT                   to glycosyl hydrolase family 16. The 141 C-terminal amino
FT                   acid sequence is composed of three reiterated amino acid
FT                   sequences and binds to pachyman, lichenan, and A. oryzae
FT                   cell walls. This domain shows a motif of
FT                   carbohydrate-binding module family 13. By proteolytic
FT                   removal of the C-terminal domain in the IAM1165 culture,
FT                   mature BglM was processed to several 27-kDa fragments
FT                   (BglLs) that hydrolyze a soluble beta-1,3-glucan."
FT                   /db_xref="GOA:Q8KKH3"
FT                   /db_xref="HSSP:1AJO"
FT                   /db_xref="InterPro:IPR000757"
FT                   /db_xref="InterPro:IPR000772"
FT                   /db_xref="InterPro:IPR008985"
FT                   /db_xref="InterPro:IPR008997"
FT                   /db_xref="InterPro:IPR013320"
FT                   /db_xref="UniProtKB/TrEMBL:Q8KKH3"
FT                   /protein_id="BAC06195.1"
FT                   /translation="MMLRKGICVVILFSLLVVLLPVNKTNAAPNWNLVWSDEFNGTSLN
FT                   RANWTPEIGTGSGGWGNNELQYYTDRAQNVQVTGGNLVITAQKESYGGMNYTSARIKTQ
FT                   DLKSFTYGKVEARIKLPSGQGLWPAFWMLGSNISSVGWPKSGEIDIMERVNNNPYVNGT
FT                   VHWDAGGHADFGRVSGNLDFSQFHVYSIEWDSKYIRWFVDGQQFNEFYIENGTGNTEEF
FT                   QRPFFILLNLAVGGNWPGSPNNSTPFPSQMLVDYVRVYQDTGASNVISDGIYTIASKAS
FT                   GKVMDVVDVSTARGAKIQQWTNYVANNQRFRVESTGDGYYKLTAVHSGKVLDVPSSSTS
FT                   TGVQLQQWDDNGSNAQRWKIVDVGGGYYKLVSKVSGLAVDVASASTADGAVVQQWTDNG
FT                   TDAQKWLFTKIN"
FT   sig_peptide     89..169
FT   terminator      1330..1359
XX
SQ   Sequence 1859 BP; 522 A; 368 C; 526 G; 443 T; 0 other;
     aagcttcagt agacggttcg cttgaagaac agctatgagt tgcactggta tccatgaagc        60
     cataatccga tgataattag gagggaacat gatgctaaga aaaggaatat gtgtagttat       120
     actgttcagt cttctggtcg ttctattgcc tgtcaacaaa accaatgccg caccgaactg       180
     gaatttggta tggagtgacg agttcaacgg aacctcgctc aatagagcca attggacacc       240
     ggaaatcggt accggcagcg gcggatgggg aaacaatgag ctgcagtatt ataccgaccg       300
     ggcacagaat gtgcaggtta ctggcggtaa ccttgtcatt acagcgcaga aggaatctta       360
     tggcgggatg aattatactt ctgcccggat caaaacccaa gacttgaaga gctttactta       420
     cgggaaagtg gaggcgcgga tcaagctgcc atcgggtcaa ggcttgtggc cggcgttttg       480
     gatgctcgga agcaacatca gttcggttgg ctggccgaaa agcggagaaa ttgacattat       540
     ggaacgggta aacaataacc cttacgtgaa tggtaccgta cactgggatg caggtgggca       600
     tgccgatttt ggacgagtgt ccggcaattt ggatttctcc caattccatg tctacagcat       660
     agaatgggac tctaaatata tccgttggtt cgtggacggc cagcagttca atgagttcta       720
     tattgagaat ggaaccggta ataccgagga attccagcgc ccgttcttta tattattgaa       780
     tctggcggtc ggcggaaatt ggcctggaag tccgaacaat tccacaccgt tcccatcgca       840
     gatgctggtg gattatgtac gcgtctatca ggacacgggt gcatcaaacg tgatcagtga       900
     tgggatttac acgatcgctt ccaaggcgag cggcaaagtg atggatgttg tggatgtctc       960
     cacggcaagg ggagccaaga tacagcaatg gaccaactac gtcgccaata atcaaagatt      1020
     cagagtagaa agcaccggag acgggtacta caagctcaca gccgtacaca gcggaaaagt      1080
     tctcgacgtg ccgagttcat ccacatccac tggcgttcag ctgcagcaat gggatgataa      1140
     tggatcgaat gcgcagcgat ggaagatcgt tgatgtgggc ggagggtatt ataaacttgt      1200
     gtccaaggtg agcggactgg cagtcgatgt agccagtgct tccacagcag atggggctgt      1260
     tgtccagcag tggacggaca atgggacgga tgcacaaaag tggttgttca ccaaaattaa      1320
     ctaaacttga acaagcagct ccgtgagggg ctgcttgttt gtgtgagctt aacggaagca      1380
     tcgaatggct tatgctcggc ggggccaagg cgacgggaga agcggggaac gattgaatta      1440
     ggggaatatg ccgattgtta atgggaaaat cggttatacg cagtaactta tccaatcgag      1500
     cagtccagca atggacttgc tcgatttgtc tgtagggttg atttttactg acatgaagat      1560
     ggggtattga cttgagcctt gcttcacgac atggtatatt gtgaagttac cggcaaaatt      1620
     cgtcgtggaa aatgagggga tgatcttaag ctaaacgcaa gtggagaaga gagaccatgg      1680
     tagttatccg gattaacatt caaaccatat gatattacgg ctgaaatcgt tggttaatgt      1740
     caaaaacagg tggcgcaatt gcgttgttgt ttcatatatt taaagagact gaagagtcga      1800
     agaagggagg aagccaatga acttaaaatg gaagtcatat ctcccgctgc tatggatcc       1859
//