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EBIDatabasesDatabase BrowsingDbfetch

EBI Dbfetch

ID   AB073908; SV 1; linear; mRNA; STD; PLN; 1378 BP.
XX
AC   AB073908;
XX
DT   25-OCT-2002 (Rel. 73, Created)
DT   14-APR-2005 (Rel. 83, Last updated, Version 3)
XX
DE   Coptis japonica comt mRNA for columbamine O-methyltransferase, complete
DE   cds.
XX
KW   .
XX
OS   Coptis japonica (Japanese goldthread)
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliophyta; eudicotyledons; Ranunculales; Ranunculaceae;
OC   Coptis.
XX
RN   [1]
RP   1-1378
RA   Sato F.;
RT   ;
RL   Submitted (05-NOV-2001) to the EMBL/GenBank/DDBJ databases.
RL   Fumihiko Sato, Kyoto University, Graduate School of Biostudies, Division of
RL   Integrated Life Sciences; Kitashirakawa, Sakyo, Kyoto, Kyoto 606-8502,
RL   Japan (E-mail:fumihiko@kais.kyoto-u.ac.jp,
RL   URL:http://www.lif.kyoto-u.ac.jp/labs/callus/index.html,
RL   Tel:81-75-753-6381, Fax:81-75-753-6398)
XX
RN   [2]
RX   DOI; 10.1046/j.1432-1033.2002.03275.x
RX   PUBMED; 12423366.
RA   Morishige T., Dubouzet E., Choi K.-B., Yazaki K., Sato F.;
RT   "Molecular cloning of columbamine O-methyltransferase from cultured Coptis
RT   japonica cells";
RL   Eur. J. Biochem. 269(22):5659-5667(2002).
XX
FH   Key             Location/Qualifiers
FH
FT   source          1..1378
FT                   /organism="Coptis japonica"
FT                   /mol_type="mRNA"
FT                   /country="Japan:Kyoto"
FT                   /clone_lib="pDR196 cultured Coptis cell cDNA"
FT                   /cell_line="156-1"
FT                   /db_xref="taxon:3442"
FT   CDS             57..1112
FT                   /codon_start=1
FT                   /transl_table=1
FT                   /gene="comt"
FT                   /product="columbamine O-methyltransferase"
FT                   /EC_number="2.1.1.118"
FT                   /db_xref="GOA:Q8H9A8"
FT                   /db_xref="HSSP:1FP2"
FT                   /db_xref="InterPro:IPR011991"
FT                   /db_xref="UniProtKB/Swiss-Prot:Q8H9A8"
FT                   /experiment="experimental evidence, no additional details
FT                   recorded"
FT                   /protein_id="BAC22084.1"
FT                   /translation="MDTPNTFQNDDEIKAQAQVWKHMFGFAETIMLRSTVSLGIPDIIH
FT                   NNGPVTLSQLVTHLPLKSTSIDRFHHFMRYLVHMQLFTISTDQITKEDKYELTPASKLL
FT                   VHGHQKSLAPYVMLQTHPEEFSVWSHVINVLDGKKPYWESNDTSMYEKTEGDPEINEIL
FT                   NDAMTSHSTFMLPALVSGLMKENVLDGVASIVDVGGNSGVVAKGIVDAFPHVKCSVMDL
FT                   NHVIERVIKNPKLDYVAGDMFTSIPNADAILLKSTLHNYEDDDCIKILNIAKEALPSTG
FT                   GKVILVEIVVDTENLPLFTSARLSMGMDMMLMSGKERTKKEWEDLLRKANFTSHQVIPI
FT                   MAIESIIVAYS"
XX
SQ   Sequence 1378 BP; 437 A; 231 C; 282 G; 428 T; 0 other;
     cattcattca tttatatatt atctgatata ttaggagtaa atattgttct aagggcatgg        60
     atactccgaa cacatttcag aatgatgatg aaataaaagc tcaagcccag gtatggaaac       120
     acatgtttgg ttttgctgaa acaattatgc taaggagcac agttagtctt gggatacctg       180
     acataattca caacaacggc cctgtcacat tatcccaatt agtaacacat ctgccactca       240
     aatccactag cattgacaga tttcatcact tcatgaggta tttagtacac atgcaactct       300
     tcacaatctc tacagatcag atcactaagg aagataaata tgagttaact cctgcatcaa       360
     aacttcttgt tcatggtcac caaaagagct tagcccctta tgtaatgttg caaacacatc       420
     cggaagagtt ctcagtttgg agtcatgtaa ttaatgtctt agatgggaaa aagccttact       480
     gggaatcaaa cgatacctcg atgtacgaaa aaaccgaagg ggatccagaa attaatgaga       540
     ttctcaatga tgctatgaca agccatagta cgttcatgtt gcctgcactt gtatctggtt       600
     tgatgaagga aaatgttctt gatggtgttg cctcaattgt agatgtaggt gggaattcag       660
     gtgttgttgc taagggaatt gttgatgctt tcccacatgt gaagtgcagt gttatggact       720
     taaatcacgt tatagaaaga gttataaaga atccaaagtt ggattatgtg gctggggaca       780
     tgttcacttc tattcctaat gctgatgcaa tcttattgaa gtctacgctc cacaattacg       840
     aggatgatga ctgcatcaag attttaaaca tagccaagga ggcactacct tcaactggag       900
     ggaaggtgat attggttgaa attgtagtgg acaccgagaa tttaccactg ttcacttctg       960
     caagattgag tatgggtatg gacatgatgt taatgagtgg aaaagaaaga acaaagaaag      1020
     aatgggaaga tctcttacgc aaagcgaatt ttacttctca ccaagtaata ccaataatgg      1080
     ctattgaatc aattattgtg gcatactctt gaaatagccc aagaaacaat tattgtggtg      1140
     tgttattgaa atagcccaag aaagaaataa ggctatgttt cgcggcagaa gattttgtat      1200
     tgcttaacac tgagtaggtg ttccgattct gcatatgtat tacataaaat atgattaatc      1260
     tggaagttgt ttggtcctca gttattctgt catggtttta gtttgtcctg tgtttggtta      1320
     acactgtaat ttatttatca tgaacaacaa attatactat cctgaattgt ttgtcttt        1378
//
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