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EBI DbfetchID AB063629; SV 1; linear; genomic DNA; STD; PRO; 8167 BP. XX AC AB063629; D13762; XX DT 26-DEC-2001 (Rel. 70, Created) DT 14-DEC-2007 (Rel. 94, Last updated, Version 5) XX DE Pseudoalteromonas piscicida chiD, cbp1, chiA genes for chitinase D, DE chitin-binding protein 1, chitinase A, complete cds. XX KW . XX OS Pseudoalteromonas piscicida OC Bacteria; Proteobacteria; Gammaproteobacteria; Alteromonadales; OC Pseudoalteromonadaceae; Pseudoalteromonas. XX RN [1] RP 1-8167 RA Tsujibo H.; RT ; RL Submitted (22-JUN-2001) to the EMBL/GenBank/DDBJ databases. RL Contact:Hiroshi Tsujibo Osaka University of Pharmaceutical Sciences, RL Department of Microbiology; 4-20-1 Nasahara, Takatsuki, Osaka 569-1094, RL Japan XX RN [2] RX DOI; 10.1128/AEM.68.1.263-270.2002 RX PUBMED; 11772635. RA Tsujibo H., Orikoshi H., Baba N., Miyahara M., Miyamoto K., Yasuda M., RA Inamori Y.; RT "Identification and characterization of the gene cluster involved in chitin RT degradation in a marine bacterium, Alteromonas sp. strain O-7"; RL Appl. Environ. Microbiol. 68(1):263-270(2002). XX RN [3] RX PUBMED; 8416892. RA Tsujibo H., Orikoshi H., Tanno H., Fujimoto K., Miyamoto K., Imada C., RA Okami Y., Inamori Y.; RT "Cloning, sequence, and expression of a chitinase gene from a marine RT bacterium, Altermonas sp. strain O-7"; RL J. Bacteriol. 175(1):176-181(1993). XX FH Key Location/Qualifiers FH FT source 1..8167 FT /organism="Pseudoalteromonas piscicida" FT /strain="O-7" FT /mol_type="genomic DNA" FT /note="originally submitted as 'Alteromonas sp. O-7'" FT /db_xref="taxon:43662" FT RBS 524..529 FT /inference="non-experimental evidence, no additional FT details recorded" FT CDS 537..3650 FT /codon_start=1 FT /transl_table=11 FT /gene="chiD" FT /product="chitinase D" FT /note="ChiD" FT /db_xref="GOA:Q8VLQ3" FT /db_xref="HSSP:1ITX" FT /db_xref="InterPro:IPR013781" FT /db_xref="UniProtKB/TrEMBL:Q8VLQ3" FT /protein_id="BAB79618.1" FT /translation="MKQHVKTLKLSQMASILGAIFCANSYAYNCDGLPEWSQSSAYNGG FT AQVQKSQQAFEAKWWTQADPVTHSGQWDDWKKLGDCDSVVTNTPPTVQSLLPADGSAFN FT ENDSVAISVNATDSDGQVTQVEFFVGGTSVGIDTTATGDNFSFNWSATAGTHAISVVAT FT DDKQAVSNTLITNIIVNTTGNQLPTANLSLGGGNNLVINQPVDIVLDGSDPDGSIAKLT FT LLINDQVAYESTTAPAQHTWTPTQLGSVTLKLVVEDNTGATAESILNANVVETPPVVNS FT CVPHGLYQTPNISPNYCDIYDENGREKMGTDHPRRVIGYFTSWRTGKNGQPSYLVDDIP FT WDKITHINYAFAHVDANNKVSIGNPTAPNNAATNMTWPGVAGAEMDPSLPYTGHFNLLN FT KFKKQHPDVKTLISVGGWAETGGYFDETGKRIASGGFYTMTTNADGSVNHAGIDAFVAS FT SVEFIRKYNFDGVDIDYEYPSSMNDSGHPDDFAISNPRRAALNASYQVLMKKLREALDQ FT AGQQDNTHYLLTIASPSSGYLLRGMETFQVTKYLDYVNIMSYDLHGAWNQHVGHNASLF FT DTGEDSELKQWNVYGTKEFEGIGYLNTDWAVKYFRGSVSAGRINIGIPYYTRGFRDVTG FT GTNGLWGQAALPNQADCPPGTGSGEKNKCGNGAVGIDNLWHDKNDVGLEVPAGSNPLWH FT VKNLQNGVLGSYLSDYGLDPVNNPEHQISGTYARHYDSVAVAPWLWNATKNVFLSIEDE FT ESMAAKVDYVINQELGGIMFWELAGDFDYDVSKNEYFMGSSLTSLAYNKFNQSGTPYSV FT KQGNKTFVKPASAVDVTFVAKDFPAGDANYPIAPTFAFTNNSAIDLSGAKISFDVPVAT FT SAIFKSNWNAQEKLGMAVDVNGSNAAGNNIGGFENEFHRFSITLVNEWGGTPKSFAPGE FT TINAQVMYYMPISGPVNFVVEKDGNRYAFSSEYSDLPIATPGTGPTDPVTSCKGTPISQ FT IVVYPNLPQGTHAAQGDLIVEGNAVYEAKWWSNKQPSVSGDYTKACNL" FT sig_peptide 537..617 FT terminator 3673..3686 FT /gene="chiD" FT RBS 3683..3686 FT /inference="non-experimental evidence, no additional FT details recorded" FT CDS 3696..5123 FT /codon_start=1 FT /transl_table=11 FT /gene="cbp1" FT /product="chitin-binding protein 1" FT /note="Cbp1" FT /db_xref="GOA:Q8VLQ2" FT /db_xref="InterPro:IPR000601" FT /db_xref="UniProtKB/TrEMBL:Q8VLQ2" FT /protein_id="BAB79619.1" FT /translation="MNSLHRKYALGLFGATSALISSAVSAHGFMDFPKARQAICQAQGG FT YWWPDDGSNIPNLACRAAFLDSGHFQFVQEHEFSTNTADYNNLEAVKANIPNGTLCSAG FT DPNKSGMSVVSPHWQRTVVEPNAQNKLAISFRATTPHNPSFWQFFLSKPNYDGDTSPLT FT WNDLELINEFGNIDFVVDPEGKRFYKMEIDIPQGREGNAVLYTRWQRFDVAGEGFYNCS FT DITITSTGTPPEWVSSGYFLKQGQDAKAGDSVWFRTFDQSGNELISHNLNVTSTNASTW FT QAELALYLNSNYGNKVNIGVKQPDGSIAFDANALLANEVFLPHAAYTSNLSVVKGGDNT FT APTVNPISNQSIAENSQATVHAHAFDDQNDPLTFIWSIPAPLSFSGEGDTISISTPEVT FT ADQTFEAELNVTDGKASTTVSFSITVQDSTNNQYPAWNSTTTYVAGDRVTHQQKVYEAK FT WWTQGEEPGASDVWKAI" FT sig_peptide 3696..3773 FT terminator 5129..5145 FT /gene="cbp1" FT RBS 5163..5167 FT /inference="non-experimental evidence, no additional FT details recorded" FT CDS 5176..7638 FT /codon_start=1 FT /transl_table=11 FT /gene="chiA" FT /product="chitinase A" FT /note="ChiA" FT /db_xref="GOA:P32823" FT /db_xref="HSSP:1EDQ" FT /db_xref="InterPro:IPR013540" FT /db_xref="UniProtKB/Swiss-Prot:P32823" FT /protein_id="BAB79620.1" FT /translation="MKLNKITSYIGFALLSGGALAAPSTPTLDWQPQQYSFVEVNVDGL FT GSYKQLVKAKDVVDISIKWNAWSGSGGDNYKVYFDDLLVNQGSLPAGTKSGVVQFPYTK FT SGRHQLYLELCEGTVCARSAGKEIVIADTDGAHLAPLPMNVDPNNRNNGTIPGRVTGAY FT FVEWGIYGRNYDVTKIPAHNLSHILYGFIPICGPNESLKSIEIGNSWRALQTACADSQD FT YEVVIHDPWAAVQKSMPGVDAKDPIRGVYSQLMALKQRYPDLKILPSVGGWTLSDPFHG FT FTNKANRDTFVASVKQFLKTWKFYDGVDIDWEFPGGDGPNPDLGDPINDGPAYVALMQE FT LRAMLDELEAETGRQYELTSAIGAGYDKIEDVDYQAAQQYMDYIFAMTYDFYGAWNNET FT GHQTGIYCGSHLSTDECNGTGVDDNGVPRKGPAYTGDHAIQLLLQQGVQPSKLVMGVAM FT YGRGWEGVLDANAAIPGNPMTAPGNGPLTGSTSEGVWEPGIMDYKAIAANAVGQGGSGV FT NGYEVGYDEQAQAAYVWNRSNGKLITYDSPRSVIAKGQYANTHQLAGLFGWEIDADNGD FT ILNAMYDGLTAGEIPNRAPTIGVSGPINVTSGQVVNVDAQASDLDNDPLTYSWVAAPGL FT ALSANNTAAVAVTAPSVAQQTSYDLTVTVNDGALSTTKTIVVVVNPEGANAAPVVTPVS FT DISVNEGASATVNVSATDPEGAALSYSWSVPAELSVANGSSATITAANVTADTTVPVTV FT TVSDGVNAVDTTFNVTIKDGAEYPTWDRSTVYVGGDRVIHNSNVFEAKWWTQGEEPGTA FT DVWKAVTN" FT sig_peptide 5176..5238 FT terminator 7748..7768 FT /gene="chiA" XX SQ Sequence 8167 BP; 2402 A; 1751 C; 1843 G; 2171 T; 0 other; ctgcagagtt cagcgtgtat tccattttga cggaataagc gatttaacga ccgtatcaca 60 ttagtctcat catcgagaca aagcactcgt atcgcttcat ttgaagtcgt tgattgctcg 120 ccaaattcca aagttcaatc tcctaaatga attaatttaa acttagttca aaaagcaagc 180 tacgacaaga aaccgataaa atacgcctta agtctaatat tacccttcaa cctgattaat 240 aactttttaa tatatgctta agaaacaaac tagtaactgt ataaaattta gagaactggg 300 ctagtaacta tcgcttaata ttaaataatt acagtttcat ttccatgtgt cagtgaatca 360 aaactaagca tatgaatcaa taatttagtg ttacattttg atgaaaaata tggcttaatt 420 tacgcttaca ttaacgttac tagctagaga aaaatgttgt tgccatattg attttatgtg 480 ttaggtttat gttgttattg ttaaccaaat acaataaatg gttggagaac caaactatga 540 aacaacatgt aaagaccctt aaattatcgc aaatggcttc gatcctcgga gccattttct 600 gcgctaactc atatgcttat aattgtgatg gtttacctga atggtcacaa agttcggcat 660 ataatggtgg tgctcaagta caaaaatcgc aacaagcttt tgaagcaaag tggtggactc 720 aggctgatcc agttacccat tccggacaat gggatgattg gaaaaaattg ggcgactgtg 780 acagcgttgt caccaacaca ccacctacag tacaaagctt actcccagct gacggcagcg 840 cttttaacga aaatgacagc gttgccattt ctgtaaatgc aacagatagc gatggccaag 900 taacgcaggt agagtttttt gtaggtggca cgtctgttgg tattgatacc acagcgacag 960 gtgataattt ttcatttaac tggtcagcaa ccgctggaac acacgcaatt tcagtagttg 1020 caacagatga caagcaagct gtgtccaaca cattaataac taatattatc gttaatacga 1080 caggaaatca gttacctaca gcaaaccttt cgttaggcgg aggtaataat ctagttatta 1140 atcagcctgt agacatcgtt cttgatggtt cagatccaga cggctcaatc gcaaaactaa 1200 cactgcttat taacgaccaa gtcgcatatg agagcacaac tgcaccggca cagcacactt 1260 ggacaccaac gcaactgggc tccgtaacat taaagcttgt tgttgaagat aataccggtg 1320 cgacagcaga gagtatactc aatgcaaatg tggttgaaac accgccagtg gtaaacagct 1380 gtgttccgca tggtctttat cagaccccca acatatcgcc taactactgt gatatctatg 1440 atgaaaatgg ccgtgaaaaa atgggtactg atcaccctag acgcgtcatc ggttacttta 1500 ccagttggag aacgggtaag aatggtcaac cgagctactt agttgatgat attccatggg 1560 ataagatcac ccacatcaat tatgctttcg cgcacgttga tgccaacaac aaggtttcaa 1620 taggcaatcc aactgctcct aacaatgcag cgacgaatat gacttggccg ggggttgctg 1680 gtgcagagat ggatccatct cttccttata ccggtcattt taacttgctg aacaaattta 1740 agaaacagca tcctgatgtt aaaaccttga tctcagtcgg cggttgggcg gaaactggtg 1800 gctacttcga cgaaacaggt aagcgaattg ccagcggcgg tttctatacg atgacgacca 1860 atgcagatgg cagcgtgaat cacgcaggta ttgatgcgtt tgtagcaagt tcagttgaat 1920 ttatccgtaa atacaacttt gatggtgtcg atattgacta tgaataccca tcttctatga 1980 atgactctgg tcaccccgat gattttgcga tatcaaaccc gcgtcgagca gcattgaatg 2040 cttcttatca agtattaatg aaaaaactga gagaagcatt agaccaagca ggtcaacaag 2100 acaatactca ctatttatta acgatcgcat caccatcttc tggttacctt ctacgtggta 2160 tggagacatt ccaagtcact aagtatctag actacgtcaa tattatgtct tatgacttac 2220 acggagcgtg gaaccagcac gttggccata acgcatcgct attcgacacc ggcgaagatt 2280 ccgagcttaa acagtggaat gtttacggca ccaaagagtt tgagggaatt ggttacctta 2340 ataccgattg ggcagtcaaa tatttcagag gctctgtctc tgcaggacgc atcaacatag 2400 gtatccctta ttatactcgc ggctttagag atgtaacagg tggtacaaat ggtttgtggg 2460 ggcaagctgc attacctaac caagccgatt gtcctccggg aactggcagc ggtgaaaaga 2520 acaagtgcgg taatggtgca gtgggtattg ataacctgtg gcacgacaag aacgatgttg 2580 gcttagaagt accggcaggc tctaacccgc tttggcatgt taaaaacctt caaaatggcg 2640 tgttaggaag ttacctaagt gattatggac tagatccagt taacaaccca gagcatcaga 2700 ttagcggtac ttatgcacgc cactatgatt ccgtagcggt tgctccttgg ctttggaacg 2760 ccaccaagaa tgttttctta tccattgaag atgaagagtc aatggccgct aaagtcgact 2820 atgtgatcaa ccaagaacta ggcggaatca tgttctggga gcttgctggt gacttcgatt 2880 atgatgtaag caaaaatgaa tacttcatgg gttcatcgct aacaagctta gcctacaaca 2940 agtttaacca aagtggcacg ccatactccg ttaaacaagg aaataagacc tttgtaaaac 3000 cagcttctgc agttgatgtc acattcgttg ctaaagattt cccagctgga gatgctaact 3060 atccaattgc accgactttt gcctttacca ataactcagc gatagatttg agtggcgcaa 3120 aaatttcgtt tgatgttcct gttgcaacat cggcaatttt caaatctaac tggaatgcac 3180 aagaaaagct tggcatggca gtggacgtaa atggatcgaa tgctgcgggt aataatattg 3240 gcggcttcga aaacgaattc caccgattct cgatcacgct tgtgaacgag tggggtggta 3300 cgcctaagtc ttttgctccc ggcgaaacta tcaatgctca agtcatgtac tacatgccta 3360 tttctggccc cgttaacttt gtggttgaga aagacggtaa ccgctatgca ttttccagtg 3420 aatatagcga cctaccaatc gcaacacctg gtacagggcc tacagatccg gtaactagct 3480 gtaaaggtac acctatcagt caaattgtgg tttacccgaa cctaccacag ggcacgcatg 3540 ctgctcaagg tgatttaatt gttgaaggaa acgcggtgta tgaagcgaaa tggtggagca 3600 ataagcaacc atcagttagc ggcgattata ccaaggcttg caacttgtaa ataataaatg 3660 gggagctttg ctccttttat aaaaggcgaa taagcatgaa tagtttacac cgtaaatatg 3720 cgcttggtct ttttggcgca acctcagctt tgatttcaag cgcagtttca gctcatggtt 3780 ttatggactt tcccaaagca aggcaggcga tctgtcaagc gcaaggaggt tattggtggc 3840 cggatgatgg atcaaatatc ccaaacctag catgtcgagc ggcttttcta gactcaggcc 3900 attttcagtt tgtgcaagag catgaatttt cgaccaatac cgctgactac aataatctag 3960 aggcagtaaa agcgaatatc cccaatggta cgctttgctc agctggtgac ccaaacaaaa 4020 gtggcatgag tgttgtctca ccacactggc agcgtacagt tgtagagcca aacgctcaaa 4080 ataaacttgc cattagcttt agggcgacaa cacctcataa tcccagtttt tggcagttct 4140 tcttgtcaaa gccaaactac gatggcgata catctcctct gacttggaat gatttagagc 4200 ttattaatga atttggcaat attgatttcg ttgtggatcc tgaaggtaaa cgcttctata 4260 aaatggaaat agatataccg caaggccgtg agggcaatgc agttttatat acccgttggc 4320 agcggtttga tgtcgctgga gagggatttt acaattgctc tgatatcaca ataacctcaa 4380 caggcacgcc accagaatgg gtgagcagcg gctatttctt aaagcaaggg caagatgcta 4440 aagccggtga tagcgtgtgg tttaggacgt ttgatcaatc cgggaatgaa ttaattagcc 4500 ataacttgaa tgtaacctcc actaatgcat caacatggca agctgagttg gctctttacc 4560 ttaatagcaa ttacggaaat aaggtcaaca ttggtgttaa gcaaccggac ggttcaattg 4620 cgtttgatgc aaacgcctta ttagcaaacg aagtattttt gccacatgcc gcttacacca 4680 gcaatcttag cgttgtaaaa gggggtgaca acaccgctcc tacggtaaat ccaatttcta 4740 accaaagcat cgcagaaaat agccaagcga cggtgcatgc tcatgcgttt gacgaccaaa 4800 atgacccact aacattcatt tggagtattc ctgcaccgct tagctttagt ggcgaaggtg 4860 acacgatttc catctcaaca cctgaagtga cggctgatca aacctttgag gctgaactta 4920 atgtcaccga tggcaaagca agcacgaccg taagtttctc aattaccgtt caagatagca 4980 ccaataacca atacccagct tggaatagta caactaccta tgtcgcaggc gacagagtaa 5040 cgcatcaaca gaaagtgtat gaagctaagt ggtggacgca gggagaagag cccggcgcat 5100 ccgatgtttg gaaagcaatt taaagaaaac ccaaattaat tgggtacaac atgtaactac 5160 aataaggttg aagatatgaa acttaataaa ataaccagct atataggatt tgccttactg 5220 agtggcgggg cacttgcagc cccttcaaca ccaacattag attggcagcc acaacagtat 5280 tcgttcgttg aagttaacgt agatgggctt ggctcttaca agcaactggt aaaagccaaa 5340 gatgttgtcg atattagcat caaatggaat gcatggagtg gctctggcgg tgataactat 5400 aaagtatatt ttgatgatct cctcgtaaac caaggcagct tgccagctgg tactaaaagt 5460 ggggtagtac aattccccta cactaaatcg ggtcgccatc agctttattt agaactgtgt 5520 gaaggtaccg tatgtgcaag aagcgcaggg aaagaaatcg tcattgccga cactgacggt 5580 gcacatctag cgccacttcc tatgaatgta gatccgaaca accgtaataa cggtacaata 5640 ccaggccgtg taacgggtgc atatttcgtc gaatggggca tatatggccg taattatgac 5700 gtaactaaga tcccagctca taacttatcg cacattttat atggctttat tccgatttgc 5760 ggacctaacg aatcactcaa atccatcgaa attggtaata gttggagagc gcttcaaact 5820 gcatgtgctg actcccaaga ttatgaagtc gtcattcacg acccttgggc tgcagtgcaa 5880 aaatctatgc caggggtgga tgcaaaagat cctattcgcg gtgtatattc tcaattaatg 5940 gcgctaaaac aacgttatcc ggaccttaaa attctgcctt cagtcggtgg atggacgcta 6000 tctgacccat tccatggctt tacaaacaaa gcaaaccgcg acactttcgt ggcgtctgta 6060 aaacaatttc ttaaaacgtg gaagttttat gacggtgtag atatcgactg ggaattccca 6120 ggtggtgatg gtccaaaccc agacttgggc gatccaatta acgacggtcc agcatatgtg 6180 gcgctaatgc aagaacttcg agcaatgctg gatgagttag aggctgaaac cggacgtcaa 6240 tatgagctca cttctgcaat aggtgcgggt tatgacaaga ttgaagatgt agattaccaa 6300 gctgcccaac agtatatgga ttatatcttt gcgatgacct atgacttcta tggtgcttgg 6360 aacaatgaaa cgggtcatca aaccggtatc tattgtggct ctcatctcag cacagacgaa 6420 tgtaacggta caggcgttga cgacaatgga gtgccacgta aaggcccagc atatactggc 6480 gaccatgcaa ttcagttgtt acttcaacaa ggtgtgcagc cctctaagct cgttatgggc 6540 gttgcaatgt acggccgcgg ctgggaaggt gtgctagatg caaatgcagc cattccaggt 6600 aatccgatga cggcgccggg taatggccca ttgacaggtt ctacaagtga aggtgtttgg 6660 gagccgggca ttatggatta caaggctatt gcagcaaacg ctgtaggcca aggtggttca 6720 ggcgttaatg gttatgaagt aggctacgat gagcaagcac aagcagcata tgtttggaac 6780 agaagtaacg gtaaactcat cacttacgat agcccacgca gcgttatcgc aaaaggccag 6840 tatgcaaaca ctcatcaact agctggttta tttggttggg aaattgacgc tgataatggc 6900 gacattctaa atgcaatgta tgatggtctt acagcaggtg aaatccctaa tcgcgctcct 6960 actatcgggg tttctggacc aatcaatgtg acctcaggtc aggttgtgaa cgtcgatgcg 7020 caagcaagcg atttagacaa tgatccatta acctattcat gggttgctgc gcctggtttg 7080 gctttgtctg ctaacaacac cgcagctgtg gctgtaactg ctccttcagt cgctcaacag 7140 acaagttacg acttgactgt aacggtcaat gatggggcgt tatcaacaac caaaacaatc 7200 gttgttgttg taaacccaga aggtgcaaat gcagcgcctg tcgttacacc ggtttcagat 7260 atttcggtta acgaaggggc ctcggctact gtcaatgtgt cagcaactga ccctgaaggc 7320 gcagcactta gctatagctg gagtgtacca gccgagctaa gcgtagcaaa tggtagctca 7380 gcgactatta ctgctgcaaa tgtcacagcc gacacaaccg tcccggtaac tgttaccgta 7440 tctgacggcg ttaacgctgt tgacacaaca tttaatgtca cgattaaaga cggtgctgaa 7500 tatcctactt gggatcgatc aacagtttat gtcggtggcg accgagtaat tcataacagc 7560 aatgtctttg aagcaaaatg gtggactcaa ggtgaagaac ctggaaccgc agatgtatgg 7620 aaggcagtaa ctaactaatt cataacagca atgtctttga agcaaaatgg tggactcaag 7680 gtgaagaacc tggaaaccgc agatgtatgg aaggcagtaa ctaactaatc taaccatccc 7740 caattaatga gcttgccagt caagctcatt ttttattcac ataaacataa agttacccat 7800 tatctgcctt ttatcagcaa tttcttcttt ctttttctgc ttggtctttc ataatgacat 7860 aatgaacttc ataaaagtca taagttctgg cagtgcttat tcgtttatcc gttttattat 7920 tcgttttagt tttttcgaaa agctacgctt ccgactgcgg gtcagtcacc cctaaaaatc 7980 ggcattggct agctggccgc cttttgttat gtatggcact cgtccttatg gcatagacgt 8040 agatatcact agagcagtgt ttgagcgcgc tgggttttgt atcgagttca ttcaacttcc 8100 cttcgtgccc acgggggatc accgagcttg agaagggctt aattgatgtc ctgccatccg 8160 caagctt 8167 // |
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