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EBIDatabasesDatabase BrowsingDbfetch

EBI Dbfetch

ID   AB062961; SV 1; linear; mRNA; STD; MAM; 1892 BP.
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AC   AB062961;
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DT   13-JUN-2001 (Rel. 68, Created)
DT   06-OCT-2006 (Rel. 89, Last updated, Version 4)
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DE   Macaca fascicularis brain cDNA clone:QmoA-10162, similar to human
DE   prenylcysteine oxidase 1 (PCYOX1), mRNA, NM_016297.1.
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KW   fis (full insert sequence); FLI_CDNA; oligo capping.
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OS   Macaca fascicularis (crab-eating macaque)
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini;
OC   Cercopithecidae; Cercopithecinae; Macaca.
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RN   [1]
RP   1-1892
RA   Hashimoto K., Osada N., Hida M., Kusuda J., Sugano S.;
RT   ;
RL   Submitted (11-JUN-2001) to the EMBL/GenBank/DDBJ databases.
RL   Katsuyuki Hashimoto, National Institute of Infectious Diseases, Division of
RL   Genetic Resources; 23-1, Toyama 1-chome, Shinjuku-ku, Tokyo 162-8640, Japan
RL   (E-mail:khashi@nih.go.jp, URL:http://www.nih.go.jp/yoken/genebank/,
RL   Tel:81-3-5285-1111(ex.2120), Fax:81-3-5285-1181)
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RN   [2]
RA   Osada N., Hida M., Kusuda J., Tanuma R., Iseki K., Hirai M., Terao K.,
RA   Suzuki Y., Sugano S., Hashimoto K.;
RT   "Isolation of full-length cDNA clones from macaque brain cDNA libraries.";
RL   Unpublished.
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CC   Lab host:    TOP10
CC   Vector:      pME18S-FL3 (Acc.No. AB009864)
CC   R.  Site1:   DraIII (CACTGTGTG)
CC   R.  Site2:   DraIII (CACCATGTG)
CC   Description: 1st strand cDNA was primed with an oligo(dT) primer
CC   [ATGTGGCCTTTTTTTTTTTTTTTTT]; double-stranded cDNA was
CC   synthesized using specific 5' and 3' primers and amplified
CC   by PCR.  The PCR product was digested with SfiI and size selection
CC   was performed to exclude fragments <1.5kb.The SfiI-digested
CC   PCR product was cloned into distinct DraIII sites of pME18S-FL3.
CC   XhoI sites just outside the DraIII sites can be used to
CC   isolate the cDNA insert.  Libraries were constructed by
CC   oligo-capping method
CC   (Sugano et al., , Institute of Medical Science, University of
CC   Tokyo).
CC   Custom primer used for sequencing
CC   ( 5' end primer [CTTCTGCTCTAAAAGCTGCG];
CC   3' end primer [CGACCTGCAGCTCGAGCACA] ).
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FH   Key             Location/Qualifiers
FH
FT   source          1..1892
FT                   /organism="Macaca fascicularis"
FT                   /mol_type="mRNA"
FT                   /sex="male"
FT                   /dev_stage="adult"
FT                   /clone_lib="macaque brain cDNA library QmoA"
FT                   /clone="QmoA-10162"
FT                   /tissue_type="medulla oblogata"
FT                   /db_xref="taxon:9541"
FT   CDS             17..1534
FT                   /codon_start=1
FT                   /transl_table=1
FT                   /product="hypothetical protein"
FT                   /note="similar to human prenylcysteine oxidase 1 (PCYOX1),
FT                   mRNA, NM_016297.1"
FT                   /db_xref="GOA:Q95KC9"
FT                   /db_xref="InterPro:IPR010795"
FT                   /db_xref="InterPro:IPR013027"
FT                   /db_xref="InterPro:IPR017046"
FT                   /db_xref="UniProtKB/Swiss-Prot:Q95KC9"
FT                   /protein_id="BAB60740.1"
FT                   /translation="MGRAVAELVSSLLGLWLLLCSCGCPEGAELRAPPDKIAVIGAGIG
FT                   GTSAAYYLRQKFGKDVKIDLFEREEVGGRLATMMVQGQEYEAGGSVIHPLNLHMKRFVK
FT                   DLGLSTVQASGGLLGIYNGEALVFEESNWFIINVIKLVWRYGFQSLRMHMWVEDVLDKF
FT                   MRIYRYQSHDYAFSSVEKLLHALGGDDFLGMLNRTLLETLQKAGFSEKFLNEMIAPVMR
FT                   VDYGQSTDINAFVGAVSLSCSDSGLWAVEGGNKLVCSGLLQASKSNLISGSVMYIEEKT
FT                   KTKHTGNPTKMYEVVYQIGTETHSDFYDIVLVATPLNRKMSNITFLNFDPPIEEFHQYY
FT                   QHIVTTLVKGELNTSIFSSRPIDKFGLSTVLTTDNSDLFINSIGIVSSVREKEDPEPST
FT                   DGTYVWKIFSQETLTKAQILKLFLSYDYAVKKPWLAYPHYKPPEKCPSIILHDRLYYLN
FT                   GIECAASAMEMSAIAAHNAALLAYHRWNGHTDMIDQDGLYEKLKTEL"
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SQ   Sequence 1892 BP; 541 A; 395 C; 439 G; 517 T; 0 other;
     agagcttgtg gaggccatgg ggcgcgccgt cgcggagcta gtctcctcgc tgctggggct        60
     gtggctgctg ctgtgcagct gcggatgccc cgagggcgcc gagctgcgtg ccccgccaga       120
     taaaatcgcg gttattggag ccggaattgg tggcacttca gcagcctatt acctgcggca       180
     gaaatttggg aaagatgtga agatagacct gtttgaaaga gaagaggtcg ggggccgtct       240
     ggctaccatg atggtgcagg ggcaagaata tgaggcagga ggttctgtca tccatccttt       300
     aaatctgcac atgaagcgtt ttgtcaaaga cttgggtctc tctactgttc aggcctctgg       360
     tggcctactg gggatatata atggagaggc tctggtattt gaggagagca actggttcat       420
     aattaacgtg attaaattag tttggcgcta tggatttcag tccctccgta tgcacatgtg       480
     ggtagaggac gtgttagaca agttcatgag gatctaccgc taccaatctc atgactatgc       540
     cttcagtagt gttgaaaaat tacttcatgc tctaggagga gatgacttcc ttggaatgct       600
     taatcgaaca cttcttgaaa ccttgcaaaa ggccggcttc tctgagaaat tcctcaatga       660
     aatgattgct cctgttatga gggtcgatta tggccaaagc acggacatca atgcctttgt       720
     gggggcagtg tcactgtcct gttctgattc tggcctttgg gcagtagaag gtggcaataa       780
     acttgtttgc tcagggctcc tgcaggcgtc caaaagcaat cttatatctg gctcagtaat       840
     gtacatagag gagaaaacaa agaccaagca cacaggaaat ccaacaaaga tgtatgaagt       900
     ggtctaccaa attggaactg agactcattc agacttctat gacatcgtct tggtggccac       960
     tccattgaat cgaaaaatgt ctaatattac ttttctcaac tttgaccctc caattgagga      1020
     attccatcaa tattatcaac atatagtgac aactttagtt aagggggaat tgaatacatc      1080
     tatctttagc tctagaccca tagataaatt tggccttagt acagttttaa ccactgataa      1140
     ttcagatttg ttcattaaca gtattgggat cgtgtcctct gtgagagaaa aggaagatcc      1200
     tgagccatca acagatggaa catatgtttg gaagatcttt tcccaagaaa ctctaactaa      1260
     agcacaaatt ttaaagctct ttctgtccta tgattatgct gtgaagaagc catggcttgc      1320
     atatcctcac tataagcccc cagagaaatg cccctctatc attctccatg atcgacttta      1380
     ttacctcaat ggcatagagt gtgcagcaag tgccatggag atgagtgcca ttgcagctca      1440
     caatgctgca cttcttgcct atcaccgctg gaatgggcac acagacatga ttgaccagga      1500
     tggcttatat gagaaactga aaactgagct atgaagtgac acactctttt ttcccctcct      1560
     agttccaaat gactatcagt ggcaaaaaaa gaacaaaatc tgagcagaga tgattttgaa      1620
     ccagatagtt tgccattatc attgtttact aaaagtaatc cctgctggtc ataggaaaac      1680
     acacggttct aattaagtgt gaaggtatag ctattgcact tatgccatct caaagtattc      1740
     ttttactatc cattaggagt ttttcttaaa cttgtttgat aataagaatc acctggagtt      1800
     aggaggtggc ggttgcagtg agctgatcgc gccactgcac accagcatga gcaacaagag      1860
     caaaactcca tctcaaaaaa aaaaaaaaaa aa                                    1892
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