spacer
spacer
EBIDatabasesDatabase BrowsingDbfetch

EBI Dbfetch

ID   AB055306; SV 1; linear; mRNA; STD; MAM; 1173 BP.
XX
AC   AB055306;
XX
DT   07-FEB-2001 (Rel. 66, Created)
DT   06-OCT-2006 (Rel. 89, Last updated, Version 3)
XX
DE   Macaca fascicularis brain cDNA, clone:QflA-13427, similar to human
DE   asparagine-linked glycosylation 9 homolog (ALG9), disrupted in bipolar
DE   disorder 1, mRNA, NM_024740.1.
XX
KW   fis (full insert sequence); FLI_CDNA.
XX
OS   Macaca fascicularis (crab-eating macaque)
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini;
OC   Cercopithecidae; Cercopithecinae; Macaca.
XX
RN   [1]
RP   1-1173
RA   Hashimoto K., Osada N., Hida M., Kusuda J., Sugano S.;
RT   ;
RL   Submitted (02-FEB-2001) to the EMBL/GenBank/DDBJ databases.
RL   Katsuyuki Hashimoto, National Institute of Infectious Diseases, Division of
RL   Genetic Resources; 23-1, Toyama 1-chome, Shinjuku-ku, Tokyo 162-8640, Japan
RL   (E-mail:khashi@nih.go.jp, URL:http://www.nih.go.jp/yoken/genebank/,
RL   Tel:81-3-5285-1111(ex.2120), Fax:81-3-5285-1181)
XX
RN   [2]
RA   Osada N., Hida M., Kusuda J., Tanuma R., Iseki K., Hirai M., Terao K.,
RA   Suzuki Y., Sugano S., Hashimoto K.;
RT   "Isolation of full-length cDNA clones from macaque brain cDNA libraries.";
RL   Unpublished.
XX
CC   Lab host:    TOP10
CC   Vector:      pME18S-FL3 (Acc.No. AB009864)
CC   R.  Site1:   DraIII (CACTGTGTG)
CC   R.  Site2:   DraIII (CACCATGTG)
CC   Description: 1st strand cDNA was primed with an oligo(dT) primer
CC   [ATGTGGCCTTTTTTTTTTTTTTTTT]; double-stranded cDNA was
CC   synthesized using specific 5' and 3' primers and amplified
CC   by PCR.  The PCR product was digested with SfiI and size selection
CC   was performed to exclude fragments <1.5kb.The SfiI-digested
CC   PCR product was cloned into distinct DraIII sites of pME18S-FL3.
CC   XhoI sites just outside the DraIII sites can be used to
CC   isolate the cDNA insert.  Libraries were constructed by
CC   Sugano et al.(University of Tokyo, Institute of Medical
CC   Science). Custom primer used for sequencing
CC   ( 5' end primer [CTTCTGCTCTAAAAGCTGCG];
CC   3' end primer [CGACCTGCAGCTCGAGCACA] ).
XX
FH   Key             Location/Qualifiers
FH
FT   source          1..1173
FT                   /organism="Macaca fascicularis"
FT                   /mol_type="mRNA"
FT                   /sex="male"
FT                   /dev_stage="adult"
FT                   /clone_lib="macaque brain cDNA library QflA"
FT                   /clone="QflA-13427"
FT                   /tissue_type="frontal lobe left"
FT                   /db_xref="taxon:9541"
FT   CDS             63..659
FT                   /codon_start=1
FT                   /transl_table=1
FT                   /product="hypothetical protein"
FT                   /note="similar to human asparagine-linked glycosylation 9
FT                   homolog (ALG9), disrupted in bipolar disorder 1, mRNA,
FT                   NM_024740.1"
FT                   /db_xref="GOA:Q9BGV0"
FT                   /db_xref="InterPro:IPR005599"
FT                   /db_xref="UniProtKB/TrEMBL:Q9BGV0"
FT                   /protein_id="BAB21931.1"
FT                   /translation="MASRGARQRLKGSGGSSGDTAPAADKLRELLGSREAGGAEHRTEL
FT                   SGNKAGQVWAPEGSTAFKCLLSARLCAALLSNISDCDETFNYWEPTHYLIYGEGFQTWE
FT                   YSPAYAIRSYAYLLLHAWPAAFHARILQTNKILVFYFLRCLLAFVSCICELYFYKAVCK
FT                   KFGLHVSRMMLAFLVLSTGMFCSSSVYPSPLICWS"
XX
SQ   Sequence 1173 BP; 302 A; 257 C; 303 G; 311 T; 0 other;
     gctatgtcgc gctcaccgac ttcatagggt tccaaattct gtcttttttc cccaagcttg        60
     ccatggctag tcgaggggct cggcagcgcc tgaagggcag cgggggcagc agtggggata       120
     cggcccccgc tgcggacaag ctgcgggagc tgctgggcag ccgagaggcg ggcggtgcgg       180
     agcacaggac cgagttatct gggaacaaag caggacaagt ctgggcacct gaaggatcta       240
     ctgctttcaa gtgtctgctt tcagcaaggt tatgtgctgc tctcctgagc aacatctctg       300
     actgtgatga aacattcaac tattgggagc caacacacta cctcatctat ggggaagggt       360
     ttcagacttg ggaatattcc ccagcatatg ccattcgctc ctatgcttac ctattgcttc       420
     atgcctggcc agctgcattt catgcaagaa ttctacaaac taataagatt cttgtgtttt       480
     actttttgcg atgtcttctg gctttcgtga gctgtatttg tgaactttac ttttacaagg       540
     ctgtgtgcaa gaagtttggg ttgcacgtga gtcgaatgat gctagccttc ttggttctca       600
     gcactggcat gttttgctca tcatcagttt acccatcgcc tttgatttgc tggtcatgaa       660
     acacaggtgg aagagtttct ttcattggtc gctggtggcc ctcatactct ttctggtgcc       720
     tgtggtggtc attgacagct actattatgg gaagttggtg attgcaccac tcaacattgt       780
     tttgtataat gtctttactc ctcatggacc tgatctttat gggacatgga tggaactgaa       840
     agccattgtc ttcagcaaac taagacagaa acagataacc aaacaccacg tttcacttat       900
     gaatgggagc tgaacgatga gaacacatgg acacacggag gggaacaaca catactgggg       960
     cctgtggagg gggacggagg gagggagagc gggagagagc ataggataat agctgatgca      1020
     tgctgggctt aatatctagg tgattggttg atctgtgcag cacaccacca tggctcaagt      1080
     ttacctatgt aacaaacctg cacgtcctgc atgtgtaccc cagaacttaa aataaaaaaa      1140
     gtttaaaaac aggaaaaaaa aaaaaaaaaa aaa                                   1173
//
spacer
spacer