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EBI DbfetchID AB052134; SV 1; linear; mRNA; STD; MAM; 3160 BP. XX AC AB052134; XX DT 09-DEC-2000 (Rel. 66, Created) DT 06-OCT-2006 (Rel. 89, Last updated, Version 3) XX DE Macaca fascicularis brain cDNA, clone:QccE-18183, similar to human histone DE deacetylase 1(HDAC11), mRNA, NM_024827.1. XX KW fis (full insert sequence); FLI_CDNA. XX OS Macaca fascicularis (crab-eating macaque) OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; OC Cercopithecidae; Cercopithecinae; Macaca. XX RN [1] RP 1-3160 RA Hashimoto K., Osada N., Hida M., Kusuda J., Sugano S.; RT ; RL Submitted (05-DEC-2000) to the EMBL/GenBank/DDBJ databases. RL Katsuyuki Hashimoto, National Institute of Infectious Diseases, Division of RL Genetic Resources; 23-1, Toyama 1-chome, Shinjuku-ku, Tokyo 162-8640, Japan RL (E-mail:khashi@nih.go.jp, URL:http://www.nih.go.jp/yoken/genebank/, RL Tel:81-3-5285-1111(ex.2120), Fax:81-3-5285-1181) XX RN [2] RA Osada N., Hida M., Kusuda J., Tanuma R., Iseki K., Hirai M., Terao K., RA Suzuki Y., Sugano S., Hashimoto K.; RT "Isolation of full-length cDNA clones from macaque brain cDNA libraries."; RL Unpublished. XX CC Lab host: TOP10 CC Vector: pME18S-FL3 (Acc.No. AB009864) CC R. Site1: DraIII (CACTGTGTG) CC R. Site2: DraIII (CACCATGTG) CC Description: 1st strand cDNA was primed with an oligo(dT) primer CC [ATGTGGCCTTTTTTTTTTTTTTTTT]; double-stranded cDNA was CC synthesized using specific 5' and 3' primers and amplified CC by PCR. The PCR product was digested with SfiI and size selection CC was performed to exclude fragments <1.5kb.The SfiI-digested CC PCR product was cloned into distinct DraIII sites of pME18S-FL3. CC XhoI sites just outside the DraIII sites can be used to CC isolate the cDNA insert. Libraries were constructed by CC Sugano et al.(University of Tokyo, Institute of Medical CC Science). Custom primer used for sequencing CC ( 5' end primer [CTTCTGCTCTAAAAGCTGCG]; CC 3' end primer [CGACCTGCAGCTCGAGCACA] ). XX FH Key Location/Qualifiers FH FT source 1..3160 FT /organism="Macaca fascicularis" FT /mol_type="mRNA" FT /sex="male" FT /dev_stage="adult" FT /clone_lib="macaque brain cDNA library QccE" FT /clone="QccE-18183" FT /tissue_type="cerebellum cortex" FT /db_xref="taxon:9541" FT CDS 503..1462 FT /codon_start=1 FT /transl_table=1 FT /product="hypothetical protein" FT /note="similar to human histone deacetylase 1(HDAC11), FT mRNA, NM_024827.1" FT /db_xref="GOA:Q9GKU5" FT /db_xref="InterPro:IPR000286" FT /db_xref="UniProtKB/Swiss-Prot:Q9GKU5" FT /protein_id="BAB18987.1" FT /translation="MGLEKLHPFDAGKWGKVINFLKEEKLLSDSMLVEAREASEEDLLV FT VHTRRYLNELKWSFAVATITEIPPVIFLPNFLVQRKVLRPLRTQTGGTIMAGKLAVERG FT WAINVGGGFHHCSSDRGGGFCAYADITLAIKFLFERVEGISRATIIDLDAHQGNGHERD FT FMDDKRVYIMDVYNRHIYPGDRFAKQAIRRKVELEWGTEDDEYLDKVERNIEKSLQEHL FT PDVVVYNAGTDILEGDRLGGLSISPAGIVKRDELVFRMVRGRHVPILMVTSGGYQKRTA FT RIIADSILNLFGLGLIGPESPSISAQNSDTPLLPPAVP" XX SQ Sequence 3160 BP; 608 A; 892 C; 1006 G; 654 T; 0 other; gggcggattt ctgtctctga gtgacgactg ccagggtgtg atgggaaggg tcagccgcgc 60 agcggggcca ggggacccct gacccccgcc ctggctccgg ttgggtcccg atttgcgttt 120 ctgagcgctt gctgtgctcg gctcctcagc tgcattctct gagtcctcac aacagccaca 180 cattttgcag ccgaagcctt gtgccacaca gctgtggggg cagttattac agtgcagagg 240 tgatgcgcgg aggccttgca gccaggcaca cctgagtgca cgcccgcttc tccacagacc 300 tgcactgtga ccttgggcac acctggtctg ctctctaagc ctctggtgcg attgtggtgc 360 ctacccccag ggctgctgtg aggaccggat tggaattggg ttcaggaaaa gacaaccagt 420 gctacacaca acccagctgt accagcatgt gccagagaca cgctggccaa tcgtgtactc 480 gccgcgctac aacatcacct tcatgggcct ggagaagctg catccctttg atgccggaaa 540 atggggcaaa gtgatcaatt tcctaaaaga agagaagctt ctgtctgaca gcatgctggt 600 ggaggcgcgg gaggcctcgg aggaggacct gctggtggtg cacacgaggc gctatcttaa 660 tgagctcaag tggtcctttg ctgttgctac catcacagaa atcccccctg ttatcttcct 720 ccccaacttc cttgtgcaga ggaaggtgct gaggcccctt cggacccaga caggaggaac 780 cataatggcg gggaagctgg ccgtggagcg aggctgggcc atcaacgtgg ggggtggctt 840 ccaccactgc tccagcgacc gtggcggggg cttctgtgcc tatgcggaca tcacactcgc 900 catcaagttt ctgtttgagc gtgtggaggg catctccagg gctaccatca ttgatcttga 960 tgcccatcag ggcaatgggc atgagcgaga cttcatggac gacaagcgtg tgtacatcat 1020 ggatgtctac aaccgccaca tctaccccgg ggaccgcttt gccaagcagg ccatcaggcg 1080 gaaggtggag ctggagtggg gcacagagga tgatgaatac ctggataagg tggagaggaa 1140 catcgagaag tccctccagg agcacctgcc cgacgtggtg gtgtacaatg caggcaccga 1200 catcctcgag ggggaccgac ttggggggct gtccatcagc ccagcgggca ttgtgaagcg 1260 ggatgagctt gtgttccgga tggtccgtgg ccgccacgtg cccatcctta tggtgacctc 1320 aggcgggtac cagaagcgca cagcccgcat cattgctgac tccatcctta atctgtttgg 1380 cctggggctc attgggcctg agtcacccag catctcagca cagaactcag acacaccgct 1440 gcttccccct gcagtgccct gaccctcgct gccctgcctg tcacttggcc ccgcctatcc 1500 gccccttagt gctttttgtt ttctaacctc atggggtggt ggaggcagcc ttcagtgagc 1560 atggaggggc agggccatcc ctggctgagg cctagagctg gcccttcctc cacttttccc 1620 tgctggaagc cagaggggct cgaggcctct atgggtgggg gcagaaggca gagcctgtgt 1680 cccagggaga cccacacgga gtcacgggtc catgggtcga gggaggcagg cagttcactg 1740 agaatcggag aggacaggct aggtcccagg cacagcaagg gccctgggct tggggtgttc 1800 tggttttgag aatggcagac ccaggtcaga gtgaggaagc ttccacctcc atcctgactg 1860 gcctccctcc ctccccttgg tcgtgggatt tgctgccctc tttgccccag agctgaagag 1920 ctacgggtgc tggtgtggat ggcccaggag gtgctgcagc tagacctcca ggtggacctg 1980 gttcccaggc agcaggtgga aaccctgggc ctggaggtga ggggcggtca ggaaggggta 2040 caggtgggtt ccctcatctg cagttctccc tcagtaaagc aaggtctaga cctgccttcc 2100 caggcccttc tttggggctg aaggtgggaa ggcctgcagc cccggatcac tgccttagca 2160 gtagtcttgc ctgttcagtg caaggggcag gttttagggg aggacttctt aggtcaggga 2220 caggcctcag ccctgttgtc tccagggggc tgctgaccca ggtggggagg gggcagaaga 2280 agggtgggag acagcaggcc aggctcacag gtggaaatca gagatgcagg ctggtggcca 2340 agccaggtcc tgtaggaatc caggcccagg gagggaccca ggtactggtg aggtggggca 2400 gggtttcccg gagagagggc agtgaggatc tctatccggc ctgggggttg tgagcatagg 2460 tagccggggc agggccctgg gtgggactgt ggcctccact ggcctcacca aagtgcctgg 2520 gccccgatcc ctgtccgtgc ccagggcccc caggtgggct gggtctctgg ccttttcctc 2580 agccctactc atggggacat tcaggggcct ccaggaagtg ggcgggggaa catccacccc 2640 tgctagccag cagctgtggc cctgaccaaa tcaggggctg gggagggaaa gtgggtccgc 2700 tgagatgtcc ctgctccatc agccccctac aggacttata ttcatcacag tgaggggggc 2760 tcccactgtc tcccgccctc cctcatgctc cctctgctgc agggaaaggg ttccaagatc 2820 acaaattgtc aacaacgctg gcctcctgga ccagaccccg aggctctaac aatgcactct 2880 gagatcccta cccttgctgt tggtctctgt cgctgactcg aggcacctaa catccgttca 2940 cacccaactc aggccagcga attctggggc tcagccacag acatgatttg tcactgttga 3000 gcttctgttc ctagagaacc ctagaggctt gattggccca gtctgctgtg tgtgctggag 3060 gtgaagaatc ccgaccttct gggggtggaa ggaaatgaaa atggaaagtt tcttgtggtg 3120 caaggcctga cgtgggtagc tgctcaataa atgctagtgt 3160 // |
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