 |
|
EBI DbfetchID AB049812; SV 1; linear; genomic DNA; STD; PRO; 4440 BP. XX AC AB049812; XX DT 14-MAY-2001 (Rel. 67, Created) DT 14-MAY-2001 (Rel. 67, Last updated, Version 1) XX DE Bacillus sp. KSM-1876 pul1876 gene for alkaline pullulanase, complete cds. XX KW . XX OS Bacillus sp. KSM-1876 OC Bacteria; Firmicutes; Bacillales; Bacillaceae; Bacillus. XX RN [1] RP 1-4440 RA Hatada Y.; RT ; RL Submitted (11-OCT-2000) to the EMBL/GenBank/DDBJ databases. RL Yuji Hatada, Kao Corporation, Tochigi Research Laboratories; 2606 Akabane, RL Ichikai, Haga, Tochigi 321-3497, Japan (E-mail:306943@kastanet.kao.co.jp, RL Tel:81-285-68-7400, Fax:81-285-68-7403) XX RN [2] RA Hatada Y., Ito S.; RT "alkaline pullulanase from the alkaliphilic bacterium"; RL Unpublished. XX FH Key Location/Qualifiers FH FT source 1..4440 FT /organism="Bacillus sp. KSM-1876" FT /strain="KSM-1876" FT /mol_type="genomic DNA" FT /db_xref="taxon:72413" FT CDS 430..3858 FT /codon_start=1 FT /transl_table=11 FT /gene="pul1876" FT /product="alkaline pullulanase" FT /db_xref="GOA:Q93UZ9" FT /db_xref="InterPro:IPR019948" FT /db_xref="UniProtKB/TrEMBL:Q93UZ9" FT /protein_id="BAB47586.1" FT /translation="MKGKLKKFLVLSMVFILTFSSFGMMGTTQLVEAETKSSIEESEIP FT ENTLRIHYQNSSGDYSNLGVWTWDDVESPTDNWPSGGIPFTEEQVTDYGAYVDVPLESG FT ARNVGFLILDIVTGGKDDAGHNGGDKRLELFSPNINEVWISEGSDEISFIEPVDLPEDT FT VRVHYKKADGNYENWSLWFWGDVQSPSETQGDFPSGATGFSEEQIGKYGAYIDIPLNEG FT AEEIGLVVVNKETNEQEADRSFTQLNQFNQIFIVEGDGAVYSNPYGAIATELLSAEQLS FT DEKIVLYFSRTDELTEEALLEQVGIRDIEEHTVDVNEVEIKTDNRTVEVKGDFNLDQSP FT YTVSLGEVSVPAKIGWRLIDEMYAYDGELGAQLHQDGTATIKLWSPKAEHVQVILYDKD FT NPDDIVTEVEMKLGDRGVWEVQLTKENTGLDSLRGYYYHYEIDHDGDKKIALDPYAKSL FT SAWNSDEQGPYAKAAIVDPSSIGPELEFAHIEGFEKKEDTIIYEVHVRDFTSDPHIADE FT LTAQFGTFASFVDKLDYIEDLGVTHIQLLPVMSYFFANEFKNDERMLDFSSTNTNYNWG FT YDPQSYFALTGMYSEDPTDPELRIKEFKKLIDEIHSRGMGVVLDVVYNHTARVGIFEDL FT VPNYYHFMDADGTPRTSFGGGRLGTTHEMSRRILVDSITYWVEEFKIDGFRFDMMGDHD FT AESIQIAYDKAKEINPNIVMIGEGWITYAGDEDDPNVQAADQHWMQYTESVGVFSDEFR FT NELKSGFGHEGEPRFLTGGARNIDLIFDNIKAQPHNFIADDPGDVVPYIEAHDNLTLHD FT VIAMAIQKDPDDHQEEIHQRIRLGNLMTLTSQGIAFLHAGQEYGRTKQFRAETSEPPYK FT STYMTDENGEPFRYPYFIHDSYDSTDIINRFDWERATNADAYPIQNLTREYTTGLIELR FT RSSDAFRLGTKDLVDEKVTQLNIPEIEETDLVVAYRIEATTGEAFYVFVNADDEERTLT FT LEEDLTVGEFVVDGKTAGVTAIAEPQGVELSAEQIKLDPLTAAIVQVGGIEAPEKPTPP FT KEEPKEPKEPKEPKEPKEPKDPKDPKDPKDPLDPPGTDDQNGETPKGNEEKKEEQKGDN FT LPNTATSNYQFIALGIALVLMATAIYWWKRKRQVA" XX SQ Sequence 4440 BP; 1493 A; 698 C; 1007 G; 1242 T; 0 other; tttttaataa aacaaattga ctgtacggct atcaagagat gaaccatatt tttaggtttc 60 aagctatcat aggtatgttg tacataagac agatgaccta tttaaatctc ttttagtaga 120 tgaccttgac taggcatagt gatcaaatta tactaaaatt atgaaaacga ttgcactaaa 180 tcatgcaaca acaccatgat tttccatttt tgtacaagtt tcatttgaag aaggtttaca 240 tgagggaata gaagcaagtc gtgtatattc ataaaactct gcaatctttc acacaaatga 300 aagcgtttca cggttgctat tctatcattc actcacatta tgaaagcgtt ctctaatttt 360 tcgtttttat aaagaggaat gactagattc atagtaataa attatttaca taaaaagggt 420 gtgagtaagt tgaagggaaa gctgaagaaa tttttagttc ttagcatggt ttttatcctt 480 actttttcaa gctttggaat gatgggaacg actcagttgg ttgaagcaga aacaaaaagt 540 tcaatcgaag aatcagaaat tccagagaat acgttaagaa ttcactatca aaacagtagt 600 ggagattatt caaatttagg tgtatggaca tgggatgatg tggaatctcc tacagacaat 660 tggccaagtg ggggaattcc ttttactgaa gaacaagtaa cagattatgg tgcatatgta 720 gatgttccgt tagaatcagg tgctagaaac gtaggattcc taattcttga tattgtgaca 780 ggtgggaaag atgatgcagg tcataacggt ggagataaac gattagagct tttttccccc 840 aatattaatg aagtgtggat ttcagaaggg tctgatgaga tctcattcat tgagcctgtt 900 gatttaccgg aagatacagt aagggttcat tataaaaaag ctgatggtaa ttatgagaat 960 tggagcttgt ggttttgggg agatgttcaa agcccatctg agactcaagg tgactttcca 1020 tcaggggcta caggtttctc tgaagagcaa atagggaagt atggagcata tatcgatatt 1080 cctttaaatg aaggagcaga agaaattggg ctggttgttg tgaataaaga gactaacgag 1140 caagaagctg atcgctcatt tacgcaatta aatcaattta atcaaatatt tatagttgaa 1200 ggtgatgggg ccgtgtattc aaacccgtat ggtgcgattg caacagaatt actttctgct 1260 gagcagttat ccgatgagaa aatcgtctta tatttttcta gaacggatga gttaacagag 1320 gaagctctgc ttgaacaagt gggcattcga gatatcgaag agcacacggt agatgttaat 1380 gaagtagaaa ttaaaacaga taatcgtacg gtagaagtga agggagattt taacctcgat 1440 caatctccat atacggtaag tcttggcgaa gtgagtgttc ctgctaagat tggctggagg 1500 ctgattgatg agatgtatgc ctatgatgga gagttagggg cacagttaca ccaagatgga 1560 acagctacga ttaagctgtg gtcaccaaaa gcagagcatg ttcaggtgat tttgtacgat 1620 aaagataacc cagatgatat cgtaacagag gttgaaatga agttaggtga tcgtggagtt 1680 tgggaagtgc aattgactaa agaaaacacg ggtctagata gtctgagagg ctactactat 1740 cattatgaaa ttgatcatga tggagataaa aaaattgctt tagatccata tgctaaatct 1800 ttatcggctt ggaatagtga tgaacaaggt ccatatgcga aagcagcgat tgtcgatccg 1860 agctcaattg gacctgagtt agaatttgct catattgaag gctttgagaa aaaagaagat 1920 acaattattt atgaagttca tgtgagagat ttcacttcag accctcatat tgccgatgag 1980 ttaaccgctc aatttggtac atttgcaagc tttgtagata agcttgatta tattgaagac 2040 ttgggtgtga cacatattca gcttttacca gttatgagtt atttctttgc gaacgagttt 2100 aagaatgatg aaagaatgct cgacttttct tcgacgaata caaattataa ttggggatat 2160 gacccgcaaa gttatttcgc tctaacgggc atgtattcag aagatccaac agaccctgaa 2220 ttacgcatta aagagttcaa gaaattaatt gatgaaattc acagtcgtgg aatgggtgtt 2280 gttttggatg ttgtgtataa tcacacagca agagttggta tctttgaaga tttagttcca 2340 aattattatc attttatgga tgctgatggt acgccaagaa caagctttgg tggtggacgc 2400 ctcggtacaa cacacgagat gtcacgtcga attctcgttg attccattac ctattgggta 2460 gaagagttta agattgatgg attccgtttt gatatgatgg gcgatcatga tgcagagtca 2520 attcaaattg cctatgataa agctaaagaa atcaatccga atattgtgat gattggtgag 2580 gggtggatta cgtacgctgg tgatgaagat gacccgaatg tacaagctgc tgatcagcat 2640 tggatgcagt atacagaaag tgtcggtgta ttttctgatg agttccgtaa tgagttaaag 2700 tctggttttg gccatgaagg tgagccgcgt ttcttaacgg gtggtgcaag aaatattgac 2760 ctgatttttg acaatattaa agctcaacca cataacttta tcgctgatga tccaggtgat 2820 gttgtgccgt atattgaagc acatgataat ttaactttac atgatgtcat tgcgatggcg 2880 attcaaaaag atccagatga ccatcaagaa gaaatccacc aacgcattcg tctaggaaac 2940 ttgatgacgt tgacttctca aggtattgct ttcttacatg caggtcaaga gtatggacgc 3000 acgaagcaat ttagagcgga aacatcagag ccaccgtata aatctaccta tatgactgat 3060 gaaaatggtg agcctttccg ttacccatac tttattcatg actcgtatga ttctacagat 3120 atcatcaatc gttttgattg ggaaagagca acgaatgctg atgcttatcc gattcaaaat 3180 ttaacgagag agtatacaac aggtttaatt gagttaagac gttctagcga tgctttccga 3240 ttaggaacaa aagatcttgt agacgaaaaa gtaacgcaat taaacattcc agaaattgaa 3300 gaaacagatt tagttgtggc ttatcgcatt gaggcgacaa caggtgaagc attttatgta 3360 tttgtgaatg cggatgatga agaaagaaca ttaacattag aagaggattt aaccgtgggt 3420 gaatttgtcg ttgatggaaa aacagctggt gtgacagcga ttgccgagcc acaaggggtg 3480 gagttgagtg ctgagcaaat aaagctggat ccattaactg cagcgattgt acaagtaggt 3540 ggtattgaag caccagaaaa accaacacct cctaaagaag aaccgaaaga accaaaagag 3600 ccaaaagagc caaaagagcc aaaagagcca aaagatccaa aagatccaaa agatccaaaa 3660 gatccactag acccgcctgg aaccgatgat caaaatggag aaacaccaaa aggaaacgaa 3720 gagaaaaaag aggagcaaaa aggtgataac ttgccaaata cggcgacatc taactatcaa 3780 ttcattgcct taggtatcgc acttgtatta atggcaaccg cgatttattg gtggaaaaga 3840 aaacggcaag tagcgtaaaa gaaaataata gatgtagccc tcacacatgg atgtgagggc 3900 ttttacttat gataataaag aggccctgcc gcctaagcgt aaaacgagag aaagcccgag 3960 caaacgtcga atctcggggt tgcggggaaa aaagagaaag aaagcccagc aaaaggaaag 4020 tcgcgcgtat ctatcaccag atggttgacc agctaacttc acggcacaat ctatcgtcca 4080 taagtaactt tcatataaaa ataattcata tcaatttaat aggctttaat tattgacaaa 4140 taggaaggat gaatctatag taaatcaata ggaattaaat cgtatgaact tgactgactc 4200 atcaatcagt tgacaaaaag atattagtcc ttaaagggtg tgaatagaat cgattcaaaa 4260 aaggtggatg cgactttacg aaaagagcaa cttatgaaag cagctttaca attatttgca 4320 acagatggtt accataaaac gaaggtttca gacattgtta aggtggttgg cgtagcacaa 4380 ggcactttct attggtactt ttctagcaaa gaagatattg ccttagccat tatcgctgtt 4440 // |
|