EBI Dbfetch

ID   AB030481; SV 1; linear; genomic DNA; STD; PRO; 1544 BP.
XX
AC   AB030481;
XX
DT   16-AUG-1999 (Rel. 60, Created)
DT   16-AUG-1999 (Rel. 60, Last updated, Version 1)
XX
DE   Corynebacterium sp. ALY-1 alyPG gene for polyguluronate lyase, complete
DE   cds.
XX
KW   polyguluronate lyase.
XX
OS   Corynebacterium sp. ALY-1
OC   Bacteria; Actinobacteria; Actinobacteridae; Actinomycetales;
OC   Corynebacterineae; Corynebacteriaceae; Corynebacterium.
XX
RN   [1]
RP   1-1544
RA   Matsubara Y., Kawada R., Iwasaki K., Kimura Y., Oda T., Muramatsu T.;
RT   ;
RL   Submitted (28-JUL-1999) to the EMBL/GenBank/DDBJ databases.
RL   Yasuhito Matsubara, Food Research Institute, Kagawa Prefectural Government,
RL   Food; 587-1 Goto, Takamatsu, Kagawa 761-8031, Japan
RL   (E-mail:syokuhin@mb.kagawa-net.or.jp, Tel:+81-87-881-8177,
RL   Fax:+81-87-882-9481)
XX
RN   [2]
RP   1-1544
RA   Matsubara Y., Kawada R., Iwasaki K., Kimura Y., Oda T., Muramatsu T.;
RT   "Cloning and sequence analysis of a gene (alyPG) encoding polyguluronate
RT   lyase from Corynebacterium sp. ALY-1 strain";
RL   Unpublished.
XX
FH   Key             Location/Qualifiers
FH
FT   source          1..1544
FT                   /organism="Corynebacterium sp. ALY-1"
FT                   /strain="ALY-1"
FT                   /mol_type="genomic DNA"
FT                   /db_xref="taxon:101519"
FT   RBS             487..493
FT                   /note="putative"
FT   CDS             502..1272
FT                   /codon_start=1
FT                   /transl_table=11
FT                   /gene="alyPG"
FT                   /product="polyguluronate lyase"
FT                   /function="degrade polyguluronate in alginate molecule"
FT                   /EC_number="4.2.2.11"
FT                   /note="guluronate-specific alginate lyase"
FT                   /db_xref="GOA:Q9RB42"
FT                   /db_xref="InterPro:IPR013320"
FT                   /db_xref="PDB:1UAI"
FT                   /db_xref="UniProtKB/TrEMBL:Q9RB42"
FT                   /protein_id="BAA83339.1"
FT                   /translation="MTLTRKRGLTAALTATALLVGSMVVGGSGAAAAEPCDYPAQQLDL
FT                   TDWKVTLPIGSSGKPSEIEQPALDTFATAPWFQVNAKCTGVQFRAAVNGVTTSGSGYPR
FT                   SELREMTDGGEEKASWSATSGTHTMVFREAFNHLPEVKPHLVGAQIHDGDDDVTVFRLE
FT                   GTSLYITKGDDTHHKLVTSDYKLNTVFEGKFVVSGGKIKVYYNGVLQTTISHTSSGNYF
FT                   KAGAYTQANCSNSSPCSSSNYGQVSLYKLQVTHS"
FT   sig_peptide     502..597
FT                   /experiment="experimental evidence, no additional details
FT                   recorded"
FT   mat_peptide     598..1269
FT                   /product="polyguluronate lyase"
FT                   /note="AA33-62 of the N-terminal mature peptide was
FT                   experimentally determined."
FT   terminator      1288..1325
FT                   /note="putative"
XX
SQ   Sequence 1544 BP; 290 A; 574 C; 495 G; 185 T; 0 other;
     ctcgagggca ccgcccgctg acgtcccgac cggctcccgc accagcggac ggacggcgac        60
     cggcccgagg gtccgctgga tgcccccggc gttgactcgt cttgctggtg gctcgtcaca       120
     gtgagcaccg caaccgcgac gccgcctcac gcccgaaagt atcgctcact ccgcgacaca       180
     tgtcgccgca ggccacaggc gtgcaccggg cacgacgcgg gaacagcgag tcgtcaagaa       240
     tgccgtcacc cccttgactc gacggagcac agggagcaca gtctcacccg acgctggtgg       300
     atcgatgcac tgcaatgcgg cagtgcgccg accagaccat cagcacggga ggaggagcac       360
     accaccggcc tcacgaacag ctcgctgcca agcgggcgag cgcctcggta ccggcggcag       420
     cgacgacccc ttgcgggctc gtcgcgcggt cgacgacgcc gcggcgcgat ccgaagcgaa       480
     ccaccgaagg gaacgcacca cgtgactctc acccgaaagc gcggactcac cgccgcgctg       540
     accgccacag cgctgctcgt cggaagcatg gtcgtcggag gcagcggcgc tgctgccgcc       600
     gagccctgcg actacccggc ccagcagctc gacctgaccg actggaaggt cacactgccg       660
     atcggctcgt ccggaaagcc ctccgagatc gagcagccgg ccctcgacac gttcgccacc       720
     gctccgtggt tccaggtcaa cgccaagtgc acgggcgtgc agttccgggc cgccgtgaac       780
     ggcgtgacga cgtccggctc gggctacccc cggtcggagc tccgggagat gacggacggg       840
     ggcgaggaga aggcgtcctg gtccgcgacg tcgggcaccc acacgatggt cttccgggag       900
     gccttcaacc acctccccga ggtcaagccc cacctggtcg gtgcgcagat ccacgacggc       960
     gacgacgacg tcacggtctt ccgcctggag ggcaccagcc tctacatcac caagggcgac      1020
     gacacgcacc acaagctggt gacgagcgac tacaagctca acaccgtctt cgagggcaag      1080
     ttcgtcgtga gcgggggcaa gatcaaggtc tactacaacg gcgtcctgca gaccacgatc      1140
     agccacacca gctcgggcaa ctacttcaag gcgggcgcgt acacgcaggc caactgcagc      1200
     aactcctcgc cctgcagcag ctcgaactac ggccaggtgt ccctgtacaa gctgcaggtg      1260
     acacactcct gaccgatccg tcgtgacgcc gggcgcgagg caggtcgacc tgcctcgcgc      1320
     ccggctcggt gcgcgacgag cgccgacgcc gggcgtggca ccagggaccg gacggggacg      1380
     ccgcggcccg ccgcgcgcac ccgccacccg cgcgagatcg cggcgaacgg gtgccgatgc      1440
     ctcctgctca cggcgcaggg ggagaacgac atccgcccca cccatcgatt ccctgagaga      1500
     acctcatgac ttcaccacga gcgcaggcgt ccgcagcacc cggg                       1544
//