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EBI DbfetchID AB029554; SV 1; linear; genomic DNA; STD; PRO; 4677 BP. XX AC AB029554; XX DT 03-JUL-2000 (Rel. 64, Created) DT 05-OCT-2006 (Rel. 89, Last updated, Version 4) XX DE Thermoactinomyces vulgaris alpha-amylase, glucoamylase TGA gene region (ORF DE -1, TVAII, tga, ORF +2), partial and complete cds. XX KW . XX OS Thermoactinomyces vulgaris OC Bacteria; Firmicutes; Bacillales; Thermoactinomycetaceae; OC Thermoactinomyces. XX RN [1] RP 1-4677 RA Uotsu-Tomita R., Tonozuka T., Sakano Y., Sakai H.; RT ; RL Submitted (22-JUN-1999) to the EMBL/GenBank/DDBJ databases. RL Takashi Tonozuka, Tokyo University of Agriculture and Technology, RL Department of Applied Biological Science; 3-5-8 Saiwai-cho, Fuchu, Tokyo RL 183-8509, Japan (E-mail:tonozuka@cc.tuat.ac.jp, Tel:81-42-367-5702, RL Fax:81-42-367-5705) XX RN [2] RX DOI; 10.1007/s002530100609 RX PUBMED; 11549021. RA Uotsu-Tomita R., Tonozuka T., Sakai H., Sakano Y.; RT "Novel glucoamylase-type enzymes from Thermoactinomyces vulgaris and RT Methanococcus jannaschii whose genes are found in the flanking region of RT the alpha-amylase genes"; RL Appl. Microbiol. Biotechnol. 56(3-4):465-473(2001). XX FH Key Location/Qualifiers FH FT source 1..4677 FT /organism="Thermoactinomyces vulgaris" FT /strain="R-47" FT /mol_type="genomic DNA" FT /db_xref="taxon:2026" FT CDS <1..491 FT /codon_start=3 FT /transl_table=11 FT /note="E. coli MalG-homolog protein" FT /note="ORF -1" FT /db_xref="GOA:Q08769" FT /db_xref="InterPro:IPR000515" FT /db_xref="UniProtKB/TrEMBL:Q08769" FT /protein_id="BAA97039.1" FT /translation="FPSTMTMVAIYLLLNMLHLLDTHLGLLLFYAGTQIPFNTWLVKGY FT FDTIPHSLDEAARIDGAGHLTIFWKVILPLAKPILAVVFLFNFMGPMTDFLLPQIILTS FT PEHQTLAVGLFSFINDDFKKQYTLFAAGSVLIALPITVIFLSLQRCFISGLTAGASKG" FT CDS 512..2269 FT /codon_start=1 FT /transl_table=11 FT /gene="tvaII" FT /product="alpha-amylase" FT /note="ORF 0" FT /db_xref="GOA:Q7DI93" FT /db_xref="InterPro:IPR013781" FT /db_xref="UniProtKB/TrEMBL:Q7DI93" FT /protein_id="BAA97040.1" FT /translation="MLLEAIFHEAKGSYAYPISETQLRVRLRAKKGDVVRCEVLYADRY FT ASPEEELAHALAGKAGSDERFDYFEALLECSTKRVKYVFLLTGPQGEAVYFGETGFSAE FT RSKAGVFQYAYIHRSEVFTTPEWAKEAVIYQIFPERFANGDPSNDPPGTEQWAKDARPR FT HDSFYGGDLKGVIDRLPYLEELGVTALYFTPIFASPSHHKYDTADYLAIDPQFGDLPTF FT RRLVDEAHRRGIKIILDAVFNHAGDQFFAFRDVLQKGEQSRYKDWFFIEDFPVSKTSRT FT NYETFAVQVPAMPKLRTENPEVKEYLFDVARFWMEQGIDGWRLDVANEVDHAFWREFRR FT LVKSLNPDALIVGEIWHDASGWLMGDQFDSVMNYLFRESVIRFFATGEIHAERFDAELT FT RARMLYPEQAAQGLWNLLDSHDTERFLTSCGGNEAKFRLAVLFQMTYLGTPLIYYGDEI FT GMAGATDPDCRRPMIWEEKEQNRGLFEFYKELIRLRHRLASLTRGNVRSWHADKQANLY FT AFVRTVQDQHVGVVLNNRGEKQTVLLQVPESGGKTWLDCLTGEEVHGKQGQLKLTLRPY FT QGMILWNGR" FT RBS 2331..2336 FT CDS 2341..4296 FT /codon_start=1 FT /transl_table=11 FT /gene="tga" FT /product="glucoamylase TGA" FT /function="glucoamylase" FT /note="ORF +1" FT /db_xref="GOA:Q9KWR2" FT /db_xref="InterPro:IPR012341" FT /db_xref="UniProtKB/TrEMBL:Q9KWR2" FT /protein_id="BAA97041.1" FT /translation="MANKRYLIDAVIGNDKILAVLDRYGELHRLWWPQKDYAQHVNRLS FT AGVLIPGKSKGVWWFHQPAGKHEQTYVHDAPILRTTATCREWPLEVVCEDEVVSGRNVL FT MRHYVITNCGEEEIPLQFVSYTSFHMGEMKRYNTTLFVGKKEALVHYRREFAVAVGSVL FT PTTRFAAGSNKDHIDQGLFNCNDIAMHTDGGCLWELGLCPPGESKCMTLFFAFGKTVEK FT ALEELEEARTLGFERLRSQTLADWEAYFGQAKPVQTGCEEMDRLYRRSLAVFRLMTDGE FT YGSMIAGPEFDEDFVRCGGYAYCWGRDAAYIATAIDRAGYHDRVRAFYRWAMQTQSRDG FT SWQQRHYLDGRLAPQWGLQIDETGSILWGMWQHYLLTNDRAFLDEIWESVQKAAQFLIL FT FIDPETGLPLPSRDLWEEREGEHTYSASAVCGGLDAAAAVADKLGEKRLAQNWKEAAGA FT IRRAVEEKCWDPERQVFYRGLKLMVPPEEWAHAEKEGKRGMIHVDEKGISRPMLWQDPV FT IDISLVGISVPFNLIAPDDERMVKMAEAIETHLTSPIVGGIKRYENDPYVGGNPWILTT FT LWLAQLYIKQGRIKKALNHLKWVVDHRTDLDLLPEQVDRETGKAAWVVPLTWSHAMFVL FT TVLDLLEIGAIGPKKS" FT CDS 4517..>4677 FT /codon_start=1 FT /transl_table=11 FT /note="ORF +2" FT /note="similar to sugar metabolic operon repressor (E. coli FT MalI)" FT /db_xref="GOA:Q9KWR1" FT /db_xref="HSSP:1PRU" FT /db_xref="InterPro:IPR000843" FT /db_xref="UniProtKB/TrEMBL:Q9KWR1" FT /protein_id="BAA97042.1" FT /translation="MATIKDVAKLAAVSPSTVSRVIAGSNRISLETKQRVQKAMEELNY FT VPNAIARS" XX SQ Sequence 4677 BP; 1136 A; 990 C; 1370 G; 1181 T; 0 other; tgtttccttc gacgatgacg atggtagcca tttacctttt gcttaatatg ttgcatttgc 60 ttgatacgca tctggggctt ttgctttttt atgccggaac gcaaattcct tttaacactt 120 ggcttgtgaa agggtatttt gacacgattc cccacagttt ggatgaagcg gcgcggatcg 180 atggagcggg gcatctaacc atcttctgga aagtgatatt gccgctggca aaacccattt 240 tggcagtcgt tttcctcttt aatttcatgg gtcccatgac ggactttctg ctgccgcaaa 300 ttatattgac cagcccggaa caccaaacac tggcggtagg gctcttcagt tttatcaatg 360 atgatttcaa aaagcagtat accctgtttg cggcggggtc ggtgttgatt gcattgccaa 420 ttaccgttat tttcctgtct ctgcaacgct gctttatttc cgggctcacc gcgggtgcca 480 gcaagggata acgttgaagg gggaagagtc catgctgttg gaagcgattt ttcatgaagc 540 gaaaggaagc tatgcttacc cgatttcgga aacgcaactg cgggtgagat tgcgggcgaa 600 aaaaggggac gtggtgagat gcgaggtgct ctatgcggac cgctatgctt cgccggagga 660 agaactggca catgcacttg cgggcaaagc agggtccgat gagcgatttg attattttga 720 ggctttactg gaatgttcca ccaaacgggt gaaatacgtg tttttgttga caggcccaca 780 aggggaggct gtttattttg gcgagaccgg tttttccgcc gaacggtcta aagcgggggt 840 gttccaatat gcttacatcc accggagcga agtgtttacg acaccggaat gggcaaagga 900 ggcggtgatc taccagatct ttcccgaacg ttttgccaat ggggatccca gcaatgatcc 960 gccgggaacg gaacaatggg ccaaagacgc cagacctcgg cacgacagtt tttacggcgg 1020 agatttaaag ggggtgatcg accgtttgcc atatttggag gagttgggag tgacggcgct 1080 ttattttact cccatttttg cttcgccgtc ccatcacaaa tatgatacag ctgattattt 1140 ggcgattgat ccgcaatttg gggatttgcc cacctttcgg cgtttggtgg atgaagctca 1200 ccggcgcggg atcaaaatca ttttggatgc cgtgtttaac catgccggag atcagttttt 1260 tgcgtttcgg gatgttctgc aaaagggaga acagtcgcga tataaggatt ggtttttcat 1320 cgaggatttt cccgtttcca agacgtcaag aaccaattat gaaacctttg ccgtccaagt 1380 gccggccatg cccaaattgc ggacggaaaa tccggaagtg aaggaatatt tgtttgatgt 1440 cgcgcgattt tggatggagc aggggatcga cggatggaga ctggatgtgg ccaatgaggt 1500 ggatcacgcc ttttggcgcg agtttcgccg ccttgtcaaa tccctcaatc ccgacgcctt 1560 gattgtcgga gagatttggc atgacgcttc cggctggctg atgggagacc aatttgattc 1620 ggtgatgaat tatttgtttc gcgagagtgt gatccgtttt tttgccacgg gggagatcca 1680 tgccgaaaga tttgacgccg aacttacccg ggccaggatg ctttatccgg aacaggcggc 1740 acaagggtta tggaacctcc tcgactcaca cgatacggaa cgttttttga catcgtgtgg 1800 ggggaatgag gcgaaattcc gcctggctgt tttgtttcag atgacatacc tggggacgcc 1860 gctgatttat tacggtgatg agatcgggat ggccggagcg acggacccgg attgccggcg 1920 cccgatgata tgggaggaaa aagagcagaa ccgcggcttg tttgagtttt ataaggaatt 1980 gattcgcttg cgccatcggc tggcatcgct caccagggga aatgtccggt cttggcatgc 2040 cgacaaacaa gccaatcttt atgcttttgt gcgcacggtt caagaccaac atgtcggtgt 2100 ggttctcaat aatcgtggcg agaagcaaac cgttttgcta caggtgccgg aatcgggcgg 2160 aaaaacttgg ctggattgtt tgaccggaga agaagtgcat ggcaaacaag ggcaactgaa 2220 gctgacactt cgcccttacc agggaatgat tctctggaat ggccggtaga ttttcggtgc 2280 ccaaaccagt gattgggata tgattccagg ctagtttttg gataaaatta ggaggatcaa 2340 atggcaaaca aacgttattt gattgatgca gtgatcggaa atgacaagat cttggccgtt 2400 ttggaccggt atggagaatt gcaccgcttg tggtggccgc agaaagatta tgcgcaacat 2460 gtgaaccggc tatctgccgg ggttctgatc ccaggcaaat caaaaggggt ctggtggttt 2520 catcagcctg cggggaaaca tgagcaaaca tatgttcatg atgctcccat tctaagaacg 2580 actgccacgt gcagggagtg gcctctggaa gttgtgtgcg aagatgaggt ggtttccggc 2640 cggaatgtat tgatgcgcca ttatgtgatc accaattgcg gagaagagga aatccccctg 2700 caatttgtct cctatacctc ttttcacatg ggggagatga aacgttataa taccactttg 2760 tttgtgggga aaaaagaagc gttggttcat taccggcggg aatttgccgt ggctgtcgga 2820 agtgtgttgc ccacaacccg ctttgcggcc ggttcaaaca aagatcatat cgaccagggg 2880 ctttttaact gcaacgacat tgctatgcac accgatggcg ggtgtttgtg ggaattgggg 2940 ctttgcccgc cgggagaaag caagtgcatg actttatttt tcgcctttgg caaaacggta 3000 gaaaaagcgc tggaagagtt ggaagaggca cggacgttgg gatttgaacg tttgcgaagc 3060 cagaccctgg ccgactggga agcctatttt ggacaagcaa aaccggtaca aacaggttgt 3120 gaggaaatgg atcgccttta ccgccgttcc ctcgccgttt tccggttgat gacggatggg 3180 gaatatggaa gcatgatcgc cgggcctgag tttgacgaag attttgtccg gtgcggcgga 3240 tatgcgtatt gttgggggcg ggacgctgcg tatattgcta ccgcgattga ccgcgcaggg 3300 taccatgaca gggttcgcgc tttttaccgt tgggcgatgc aaacgcaaag cagggatggt 3360 tcatggcaac aacggcatta tcttgatggc cgcctggccc cgcaatgggg gttgcaaatc 3420 gatgaaaccg gttcaatcct gtggggaatg tggcaacatt atttgctcac aaatgatcgg 3480 gcttttcttg atgagatatg ggaaagtgta cagaaagccg cgcagtttct catccttttt 3540 atcgacccgg aaaccggttt gcccttgcca agccgtgatc tgtgggagga gagggaaggg 3600 gaacacactt attccgcgtc ggccgtttgc ggtggactcg atgcggcggc ggccgtggca 3660 gataagctgg gggaaaaacg gctggcgcaa aactggaaag aggcggcagg agccatacgc 3720 cgtgcggttg aagagaagtg ctgggatccg gagcggcagg tcttttatcg tggcttaaag 3780 ctcatggttc ccccggaaga atgggcccac gctgaaaagg aaggaaaacg ggggatgatt 3840 cacgttgacg aaaagggcat ttcccgtccg atgctatggc aggacccggt cattgatatc 3900 agtttggtgg gaatcagtgt tcctttcaat ttgattgcgc cagatgatga acgaatggta 3960 aaaatggcgg aagccattga aacccatctg accagtccga tcgtgggggg catcaagcgg 4020 tatgagaatg atccgtatgt tggaggcaac ccttggattc ttaccacctt gtggctggcg 4080 cagttatata tcaaacaggg cagaataaaa aaggctctca accatctgaa gtgggtggtg 4140 gatcatcgca ccgatttgga tctgttgccg gagcaggttg accgtgagac ggggaaagcg 4200 gcttgggtcg taccgcttac ttggtcccac gccatgtttg tgttgaccgt cttggatttg 4260 ttggagattg gggcaattgg gccgaaaaaa agttgaacct gggggatttc attggaaaag 4320 gcgtgcttga ttcaattcag gcacgcctaa aaaatgatgt ttccgttggg agcccggcaa 4380 cataaagaga aatgaacaag agcccaattc aacatacaaa cacaatctca tattttttgg 4440 cgaaaaatat agtataatca atgatgaaaa cgattgcacg acttggtgaa taaattttaa 4500 aaaggggttt tcgagaatgg cgaccatcaa agatgtggca aaattggcgg ctgtttcgcc 4560 atcgactgta tcgcgtgtca tcgcgggaag caaccggatc agcttggaaa ccaaacagcg 4620 tgtgcaaaag gcgatggaag aattgaacta tgttcccaat gcgattgcgc gaagctt 4677 // |
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