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EBI DbfetchID AB028321; SV 2; linear; genomic DNA; STD; PRO; 6097 BP. XX AC AB028321; XX DT 13-MAR-2000 (Rel. 63, Created) DT 09-JUN-2005 (Rel. 84, Last updated, Version 5) XX DE Ruminococcus albus cel5D, cel9A genes for beta-1,4-endoglucanase VII, DE cellulase VI, complete cds. XX KW beta-1,4-endoglucanase VII. XX OS Ruminococcus albus OC Bacteria; Firmicutes; Clostridia; Clostridiales; Ruminococcaceae; OC Ruminococcus. XX RN [1] RP 1-6097 RA Karita S.; RT ; RL Submitted (03-JUN-1999) to the EMBL/GenBank/DDBJ databases. RL Shuichi Karita, Mie University, Faculty of Bioresouces, Department of RL Sustainable Resource Science; 1577 Kurimamachiya, Tsu, Mie, 514-8507, Japan RL (E-mail:karita@bio.mie-u.ac.jp, Tel:81-59-231-9619, Fax:81-59-231-9604) XX RN [2] RX DOI; 10.1271/bbb.64.254 RX PUBMED; 10737178. RA Ohara H., Karita S., Kimura T., Sakka K., Ohmiya K.; RT "Characterization of the cellulolytic complex (cellulosome) from RT Ruminococcus albus"; RL Biosci. Biotechnol. Biochem. 64(2):254-260(2000). XX RN [3] RX DOI; 10.1271/bbb.68.1557 RX PUBMED; 15277761. RA Taguchi H., Hagiwara D., Genma T., Karita S., Kimura T., Sakka K., RA Ohmiya K.; RT "Cloning of the Ruminococcus albus cel5D and cel9A genes encoding dockerin RT module-containing endoglucanases and expression of cel5D in Escherichia RT coli"; RL Biosci. Biotechnol. Biochem. 68(7):1557-1564(2004). XX FH Key Location/Qualifiers FH FT source 1..6097 FT /organism="Ruminococcus albus" FT /strain="F-40" FT /mol_type="genomic DNA" FT /db_xref="taxon:1264" FT CDS 649..2910 FT /codon_start=1 FT /transl_table=11 FT /gene="cel5D" FT /product="beta-1,4-endoglucanase VII" FT /note="egVII" FT /note="glycoside hydrolase family 5" FT /db_xref="GOA:Q9LCC4" FT /db_xref="HSSP:1DAV" FT /db_xref="InterPro:IPR013781" FT /db_xref="UniProtKB/TrEMBL:Q9LCC4" FT /protein_id="BAA92430.1" FT /translation="MKTKSKSALTLSMVCAMALSCMPFVEPTRAEAAFDKDAKQTVADM FT GLGWNLGNSLDSYSGTTIGGNRGSTSSETAWGNPATTKAMIDMVKESGVKTVRVPVTWY FT EHMDPYTYKIDDVWMNRVEEVVNYVLEDDMYCILNVHHDTGEKGWLKANSKDLQKKEAM FT FRSIWEQVSENFEDYGDKLVFEGFNEILDESSNQWWNPSSEACPISNELNQIFVDVVRG FT SGGNNAKRNLICNTYCAGANNEITSQFVLPKDTVSNRLIVEAHVYQPFEFTHESYPEIT FT TWTSSPLDMVLNNLNSTFAQKGIPVIIGEFGCANKNNMDEITSWAKYLVEKCTGYGMGC FT IWWDNGSQYKIYNRRTLKVSQPELLNAMLEAAGSDVPASAQKFVRGDINGDGKLNDTDL FT EMLRNYLLKKTVKISDKADINMDGKVTMADFVQMNKAILNPDPASDPRNLCANEDNWAS FT WTDTSNGGQGEMFFMDNGVSMQVDKGGKNEWDAQFFYDAITLEQGAKYQISFDYVSDSP FT QTTTFIVNQGHDDYLPYYLDSLNWTTNVQHYKATFDFTSKTDNMCRVTYNLGGSGVNVP FT YKATITNLSLIKLSGGGSSSSSSSSTQSGTSASTGTNLAADASKFSSWANTEGGASASF FT KSLSNGLQISVKNSGTEEWYVQGIYPGIKFEKGATYEISFDHQADKAVKFGVKLQQNYE FT PYGQYSNEPVYASTQKQHYSAEFTMTDPTDDNTACVFNCGALNTNATITITDLVIKKIS FT " FT CDS complement(3145..5955) FT /codon_start=1 FT /transl_table=11 FT /gene="cel9A" FT /product="cellulase VI" FT /note="egVI" FT /db_xref="GOA:Q93IE6" FT /db_xref="HSSP:1IA6" FT /db_xref="InterPro:IPR018247" FT /db_xref="UniProtKB/TrEMBL:Q93IE6" FT /protein_id="BAB64431.1" FT /translation="MGKGKFLKRSLAGLSAAAMMFTGNTAATVTAAVIDKENPNNYHHY FT AEALQLALCFYDANKCGDEVADDGYYSWRANCHVKDGYIPLQPMSPMPEIGKGKTDDQL FT QEDQGLGAGDDGKTKPNLGDTDPSLYAGVNMSQNFIDKNKKYLDPDGDGTLDLTGGWHD FT AGDHVKFGLPGSYSASTVGWGYYEFRDSYKELGLDKHVEDELHWINDYFMKVTFMDDDD FT NVIAYCYQVGEGNNDHNYWCPPELQVEDTMVASSSCAVKRPAYFATEEMPASDQCAGAA FT ASLAINYLNFKDTEPEYAKKSLKYAKALYDFAVKTHVEEWKPGEVPSCSSLGYDGGFYT FT SSYDYDELAWAAVWLYYCTEDYDYINDIIAVDETTTNAKGAHPYTGYMKRIITDTGNCW FT QNIWVHCWDTVWGGVFAKLAPVTNTARDWYIFRYNLEFWSGCAESVDSSDWGYEPVHGH FT KYFGMDDYLWNTKMTADQIADLPISETSGDFIAKSPKGWAVVSGYGSARYNTAAGLCAC FT VYAKTTNDQTFLPWARAQMEYILGKNPMGYAYEVGYGNNFASHPHHRSSHCSATQSMDD FT PITQVHTLWGALVGGPDLKDNHVDETKDYIYNEVTDDYNAGFCGDLAGLYHYYGRKGGI FT DAKENSIIKNFDMSSNAKGFDQVDENGNPLPVGYFVSGGKAQEKKDGIQLKVVLHNRTV FT DPPRFECNVKARYFFNIKELLDAGYGIDYITARIDYDQVAGYSNNKSHAELSDPVKYDD FT KGTYYVDITWKDCKFYGSRVYQFALTTKMDPEKFVYPEWDSSNDYSYSDLVSFEDDNSA FT AAITDKIALYADGKLIWGSEPDGTTPETFVNDEPTENPSAKILYGDVNCDGKVNMADVT FT AIYQYGNNKRKYPLTAQGMRNADCVDAGKGLTNDDAKAIKAVVISALKVSDLPISSTAL FT KKVSVK" XX SQ Sequence 6097 BP; 1631 A; 1356 C; 1431 G; 1679 T; 0 other; gaattcatca gctattgcct tgatgcacca gctcagaaac tcagcgtgag gtgttgttat 60 tcctttggca taggggatat ttcttttcat aaattcagcc tcgtcatcag cctcaaactg 120 aaaatgcacc cagtcatttg caaattcatg cttgggaaca catcggtaat actgcggcat 180 accctgagga taaaggaaga atgaatcctt tgggacgata atatctttgt tttccagagt 240 gaaaacagca tctgttttca gtatcagcaa aagaaagtca ccatacccgt tcggccgttc 300 aataaaaaaa tctccatcgt gtctgtgatt gaaacctaca ttgtttatta tcattctgtt 360 cacctgccgg cttgactgaa tttgatgatt tttcagaaaa gatcatctaa gttatattat 420 atatcattga aataagtgat gtcaatagta tataatattt ttagtgagaa gttttaaata 480 aatacatatt atgcacaagc tgtctgtcct gtcagctgcc cctgattgga acagacggcg 540 ctcacagtaa aggcaggctg tgagaacata ggaagaggat cgtttttcgt ctgaaggggt 600 attcagacgg aagaacaatg aaccacattt tttagaggtg aatgtataat gaagactaaa 660 agcaaaagcg cacttacact ttcaatggtg tgtgcgatgg cactctcgtg tatgcctttc 720 gtggagccta cacgtgctga agccgcattc gataaggatg ctaagcagac tgttgctgat 780 atgggtctgg gctggaacct cggcaactct ctggacagct attcaggtac gaccataggc 840 ggaaacagag gcagcacatc ctccgaaaca gcttggggaa accctgcgac caccaaagcc 900 atgatagata tggtaaagga gtcgggtgtc aagaccgtac gtgtgcctgt gacctggtat 960 gagcatatgg atccctacac ctacaagatc gacgatgtat ggatgaacag ggtcgaggaa 1020 gtagttaact atgtactcga agatgatatg tactgcatcc tcaatgttca ccatgataca 1080 ggtgaaaaag gctggctgaa agccaacagc aaagatcttc agaagaaaga agctatgttc 1140 aggtctatct gggaacaggt cagcgagaac ttcgaggatt acggcgacaa gcttgttttc 1200 gagggcttca atgaaatact tgatgaaagc tcaaatcagt ggtggaaccc aagctcggaa 1260 gcttgcccta tctccaatga gctcaatcag atatttgttg atgttgtcag aggctcaggc 1320 ggaaacaacg caaagagaaa ccttatatgc aacacctatt gtgcaggtgc aaacaatgag 1380 atcacaagtc agttcgtact cccaaaggat actgttagca acaggctgat cgttgaagct 1440 catgtttatc agccttttga gtttactcac gagagctatc ccgagataac tacatggaca 1500 agctcaccgc tggatatggt actcaataat ctcaacagca cttttgcaca gaagggtatc 1560 cctgttatta taggtgaatt cggctgtgcc aataagaata atatggatga gatcacatcc 1620 tgggcaaagt atcttgtaga aaaatgtaca ggctatggta tgggttgtat ctggtgggat 1680 aacggatcac agtataagat ctataaccgc cgtacactga aagtatctca gcccgaactt 1740 ctcaatgcta tgcttgaagc tgcaggttct gatgttcctg cttctgctca gaagtttgtc 1800 agaggcgata tcaacggtga cggaaagctc aatgatacag atctggaaat gctccgtaat 1860 tatcttctta aaaagaccgt aaagatcagt gacaaagctg atatcaacat ggacggaaaa 1920 gtgactatgg ctgatttcgt tcagatgaat aaggctatac tgaatcctga tcctgcatct 1980 gacccgagaa atctctgtgc aaatgaagac aactgggcaa gctggacgga tacttcaaac 2040 ggcggtcagg gcgagatgtt ctttatggac aatggcgttt ccatgcaggt agataaaggc 2100 ggcaagaatg agtgggacgc acagttcttc tatgatgcca taacacttga acagggtgca 2160 aagtatcaga tatcattcga ttatgtaagt gattctccgc agaccactac tttcatagtt 2220 aatcagggtc acgatgatta tctgccttac tatttagaca gccttaactg gacaacaaat 2280 gttcagcatt ataaagcgac cttcgacttt acttccaaga ctgacaatat gtgccgcgtt 2340 acctacaatc tcggcggcag cggtgtgaat gttccttaca aggctactat caccaatctt 2400 tcccttatca agcttagcgg cggaggatct tcgtcctcat catcttcttc gactcagtcc 2460 ggaacttccg cttcaacagg tacaaatctt gctgctgatg cttcaaagtt cagtagctgg 2520 gcaaacactg agggtggtgc atctgcatcg ttcaagagcc ttagcaacgg tcttcagatc 2580 tcggtaaaga attcgggtac ggaggaatgg tacgttcagg gaatataccc cggcatcaag 2640 tttgaaaaag gtgcgactta tgaaatatca ttcgatcatc aggctgataa agcagtaaag 2700 ttcggcgtta agcttcagca aaattatgag ccatacggtc agtattccaa cgagccggta 2760 tatgcttcca cacagaagca gcactattct gcagaattca caatgacaga cccgactgat 2820 gataacactg cctgtgtatt caactgcggt gctttgaata ccaacgctac gatcacgatc 2880 actgatctgg tcatcaaaaa gatctcctga ttcatcccgc atatatagat aagtacaatt 2940 atcggacaaa ccaccccctg tcgggcgaca gagggtggtt tgtttatatg ctttactata 3000 aaaatacatg gcgatacatt tgatacacca acacaaaatg ctatataaca tcatacctga 3060 cagattttcg ttatgtggtg ttatgccaaa acaaaaccac gccgtaactc ttgcgagtgc 3120 ggcgtggtac gcttttaact gttattactt aacagaaact tttttaagtg cagtagatga 3180 tatagggaga tcggatacct tgagagctga tattactaca gccttgatgg ctttagcatc 3240 atcattggta agacctttgc ctgcatcaac acagtctgca tttctcatac cctgagcagt 3300 gagaggatac ttcctcttgt tgttgccgta ctggtaaata gctgttacat cagccatatt 3360 taccttgccg tcgcagttaa catcaccgta caagatcttt gcggaaggat tttcggtggg 3420 ttcatcattt acaaaagttt cgggcgtagt tccgtcaggc tcagaacccc agatgagctt 3480 gccgtccgcg taaagtgcta tcttatcggt tatagctgca gcagagttgt catcttcaaa 3540 gcttaccagg tcggagtagc tgtagtcgtt tgaagaatcc cattcaggat atacaaactt 3600 ttcgggatcc atctttgttg tcagcgcgaa ctggtataca cggctgccgt agaacttgca 3660 gtccttccag gtgatgtcaa cgtagtaagt tcccttgtcg tcatacttga cgggatcgga 3720 aagttctgcg tgtgacttat tgttggaata gcctgctacc tggtcatagt cgatacgtgc 3780 agtgatatag tcgataccgt agcctgcatc caggagttcc ttgatattga agaagtatct 3840 tgcctttaca ttgcactcaa atctgggagg atcaacagtt ctgttgtgaa gaacgacttt 3900 gagctggata ccatccttct tttcctgtgc cttaccgcct gatacgaagt atccaacagg 3960 cagaggattg ccgttttcat cgacctgatc gaagcccttt gcatttgagg acatatcaaa 4020 gttcttgatg atgctgttct ccttagcatc tatgccgcct ttgcggccgt agtagtggta 4080 aagacctgca aggtcaccgc agaagcctgc attgtagtcg tcagttacct cgttgtagat 4140 gtagtccttg gtctcatcaa catggttgtc tttcagatcg ggaccgccta caagtgcacc 4200 ccagagggtg tgtacctgtg tgataggatc atccatgctc tgtgtagcgg agcagtgaga 4260 tgaacggtgg tgaggatggc ttgcgaagtt gttgccgtag cctacctcat aagcgtaacc 4320 catggggttc ttgccgagga tgtattccat ctgagctctt gcccagggga ggaatgtctg 4380 atcgtttgta gtcttggcat atacacaagc gcacagacct gcagctgtgt tgtaacgtgc 4440 agaaccgtat ccagatacta ctgcccagcc cttgggtgac ttagcaataa agtcgccgct 4500 tgtttcgctg ataggaaggt ctgctatctg atctgcggtc atctttgtgt tccagagata 4560 gtcgtccata ccgaagtact tatgaccgtg aacaggctca tagccccagt cggaagaatc 4620 aacactttca gcacagcctg accagaattc aaggttatag cggaagatgt accagtcacg 4680 tgctgtgttg gtaacaggag caagctttgc gaatacaccg ccccatactg tatcccagca 4740 gtgaacccag atgttctgcc agcagttgcc ggtatctgtg atgatacgct tcatatatcc 4800 ggtgtaagga tgagcgccct ttgcgtttgt agtggtttca tcaactgcga ttatatcgtt 4860 gatgtaatcg tagtcctcgg tgcagtagta aagccataca gcagcccatg ccagttcatc 4920 gtagtcatag ctggatgtat agaaaccgcc gtcatagcca aggcttgaac agctgggaac 4980 ttcgccgggc ttccattcct caacgtgggt ctttacagcg aaatcgtaca gtgcctttgc 5040 gtactttaat gactttttag cgtactcggg ctcagtgtct ttgaagttga ggtagttgat 5100 agcaagagat gcagcagcac cggcgcactg atcggatgca ggcatttctt cagtagcgaa 5160 gtaagcagga cgcttaacag cacaggatga agaagcaacc atagtatcct ctacctggag 5220 ctcgggtggg caccagtagt tatggtcatt attaccttcg cctacctgat agcaataagc 5280 aattacatta tcatcgtcgt ccatgaaagt aactttcatg aaataatcgt tgatccagtg 5340 gagctcatcc tcaacgtgct tgtcaagtcc cagttccttg tatgaatcac ggaattcata 5400 gtagccccag cccactgttg aagcggagta gcttcccgga aggccgaact tgacgtggtc 5460 acctgcatcg tgccagccgc ctgtaaggtc gagagtgccg tctccgtcag ggtcgaggta 5520 tttcttgttc ttgtcgatga aattctggga catattgacg cctgcataca gagaggggtc 5580 ggtatcaccg aggtttggct tggtctttcc gtcatcgcct gcgccaagtc cctgatcctc 5640 ctgcagctga tcgtcagttt tgcctttacc tatctccggc atggggctca tgggctggag 5700 cggtatataa ccgtccttta catgacaatt tgcacgccag gagtaatagc cgtcatcggc 5760 tacctcgtcg ccgcacttgt tagcatcgta gaaacaaagt gcaagctgca gagcttctgc 5820 gtagtggtgg tagttgttgg gattctcttt gtcgatcaca gcggcggtaa ctgtagcagc 5880 agtgtttcct gtgaacatca ttgcagcagc ggataagccg gcaagtgatc ttttaagaaa 5940 ctttcccttt cccattttaa tcttcctttc tgtcagtgtt tttcagcaga gctttctttt 6000 taagtctgtc agtacgatac tatagatatc gtttcttttc tataataagg tcaacagcat 6060 caaaaagaga tcccatgcta tcggatctga tcttatg 6097 // |
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