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EBIDatabasesDatabase BrowsingDbfetch

EBI Dbfetch

ID   AB028011; SV 1; linear; genomic DNA; STD; FUN; 604 BP.
XX
AC   AB028011;
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DT   30-NOV-1999 (Rel. 61, Created)
DT   21-JUN-2001 (Rel. 68, Last updated, Version 5)
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DE   Schizosaccharomyces pombe gene for Hypothetical protein, partial cds,
DE   clone:TC78.
XX
KW   .
XX
OS   Schizosaccharomyces pombe (fission yeast)
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Taphrinomycotina;
OC   Schizosaccharomycetes; Schizosaccharomycetales; Schizosaccharomycetaceae;
OC   Schizosaccharomyces.
XX
RN   [1]
RP   1-604
RA   Ding D., Tomita Y., Hiraoka Y.;
RT   ;
RL   Submitted (24-MAY-1999) to the EMBL/GenBank/DDBJ databases.
RL   Da-Qiao Ding, Communications Research Laboratory, Kansai Advanced Research
RL   Center; 588-2, Iwaoka, Iwaoka-cho, Nishiku, Kobe, Hyogo 651-2401, Japan
RL   (E-mail:ding@crl.go.jp, Tel:+81-78-969-2240, Fax:+81-78-969-2249)
XX
RN   [2]
RX   DOI; 10.1046/j.1365-2443.2000.00317.x
RX   PUBMED; 10759889.
RA   Ding D.Q., Tomita Y., Yamamoto A., Chikashige Y., Haraguchi T., Hiraoka Y.;
RT   "Large-scale screening of intracellular protein localization in living
RT   fission yeast cells by the use of a GFP-fusion genomic DNA library";
RL   Genes Cells 5(3):169-190(2000).
XX
CC   An S. pombe gene library in which genomic DNA fragments are fused
CC   to the 5'-end of the GFP-S65T gene was constructed. S. pombe
CC   strain was transformed with the library DNA amplified in E.coli.
CC   Plasmids of those transformants that showed interesting GFP
CC   localization were isolated, and their nucleotide sequences were
CC   determined. Detailed information and images can be seen in our web
CC   site (http://www-karc.crl.go.jp/bio/CellMagic/). Note: Only major
CC   localization is described for each clone in most cases. Because it
CC   is a random fusion of DNA fragment with the GFP gene, the
CC   subcellular localization of the fusion protein may differ from
CC   that of the full-length gene product.
XX
FH   Key             Location/Qualifiers
FH
FT   source          1..604
FT                   /organism="Schizosaccharomyces pombe"
FT                   /strain="968 h90"
FT                   /mol_type="genomic DNA"
FT                   /clone="TC78"
FT                   /note="subcellular localization of GFP fusion; Membrane and
FT                   cytoplasmic dots"
FT                   /db_xref="taxon:4896"
FT   CDS             <1..>604
FT                   /codon_start=1
FT                   /gene="SPBC1734.04"
FT                   /product="Hypothetical protein"
FT                   /db_xref="GENEDB:SPBC1734.04"
FT                   /db_xref="GOA:O74745"
FT                   /db_xref="InterPro:IPR005109"
FT                   /db_xref="UniProtKB/Swiss-Prot:O74745"
FT                   /protein_id="BAA87315.1"
FT                   /translation="HSWVYWRDVDVETAPNTILEDLMRHDKDIIVPNVYRPLPDWLGNE
FT                   QPYDLNSWAESETALQLADTLGEDDVIVEGYAEYATWRPHLAYLRDPNGDPSVEMPLDG
FT                   IGGVSIMSKAKVHLEGCEFPAFSFEKHAETEGFGKMAKRMGFSVYGLPHYVIWHIYEPS
FT                   DDDKRIMAEMERERKEREAAELEEAKKYQTAGFTQPDD"
XX
SQ   Sequence 604 BP; 168 A; 111 C; 152 G; 173 T; 0 other;
     cattcttggg tttactggag agatgttgac gtggagactg ctccaaacac tattttggag        60
     gatcttatgc gccatgataa agacattatt gttcctaacg tttatagacc cttaccagat       120
     tggttgggca atgagcaacc atatgattta aattcatggg ctgaatctga gactgctctt       180
     cagcttgcag atactttggg cgaagacgac gttattgtag aaggatacgc agagtatgct       240
     acctggcgcc ctcatttggc ttaccttcgt gatcctaatg gtgacccatc tgttgaaatg       300
     ccactggatg gtattggtgg tgtatctatt atgtctaaag ctaaagtaca cctagaaggt       360
     tgcgagtttc ccgcattttc atttgaaaag catgctgaaa ccgaaggatt tggaaaaatg       420
     gctaagcgga tggggttttc cgtttacgga cttcctcact atgttatttg gcatatttat       480
     gaacctagcg atgatgataa gcgtattatg gctgaaatgg aacgcgaaag gaaagaaagg       540
     gaggccgcag agcttgaaga ggctaaaaag tatcagactg ctggattcac gcaaccagat       600
     gatc                                                                    604
//
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