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EBIDatabasesDatabase BrowsingDbfetch

EBI Dbfetch

ID   AB027839; SV 1; linear; genomic DNA; STD; FUN; 465 BP.
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AC   AB027839;
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DT   30-NOV-1999 (Rel. 61, Created)
DT   14-APR-2005 (Rel. 83, Last updated, Version 5)
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DE   Schizosaccharomyces pombe gene for Hypothetical protein, partial cds,
DE   clone:SA03.
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KW   .
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OS   Schizosaccharomyces pombe (fission yeast)
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Taphrinomycotina;
OC   Schizosaccharomycetes; Schizosaccharomycetales; Schizosaccharomycetaceae;
OC   Schizosaccharomyces.
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RN   [1]
RP   1-465
RA   Ding D., Tomita Y., Hiraoka Y.;
RT   ;
RL   Submitted (24-MAY-1999) to the EMBL/GenBank/DDBJ databases.
RL   Da-Qiao Ding, Communications Research Laboratory, Kansai Advanced Research
RL   Center; 588-2, Iwaoka, Iwaoka-cho, Nishiku, Kobe, Hyogo 651-2401, Japan
RL   (E-mail:ding@crl.go.jp, Tel:+81-78-969-2240, Fax:+81-78-969-2249)
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RN   [2]
RX   DOI; 10.1046/j.1365-2443.2000.00317.x
RX   PUBMED; 10759889.
RA   Ding D.Q., Tomita Y., Yamamoto A., Chikashige Y., Haraguchi T., Hiraoka Y.;
RT   "Large-scale screening of intracellular protein localization in living
RT   fission yeast cells by the use of a GFP-fusion genomic DNA library";
RL   Genes Cells 5(3):169-190(2000).
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CC   An S. pombe gene library in which genomic DNA fragments are fused
CC   to the 5'-end of the GFP-S65T gene was constructed. S. pombe
CC   strain was transformed with the library DNA amplified in E.coli.
CC   Plasmids of those transformants that showed interesting GFP
CC   localization were isolated, and their nucleotide sequences were
CC   determined. Detailed information and images can be seen in our web
CC   site (http://www-karc.crl.go.jp/bio/CellMagic/). Note: Only major
CC   localization is described for each clone in most cases. Because it
CC   is a random fusion of DNA fragment with the GFP gene, the
CC   subcellular localization of the fusion protein may differ from
CC   that of the full-length gene product.
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FH   Key             Location/Qualifiers
FH
FT   source          1..465
FT                   /organism="Schizosaccharomyces pombe"
FT                   /strain="968 h90"
FT                   /mol_type="genomic DNA"
FT                   /clone="SA03"
FT                   /note="subcellular localization of GFP fusion; Nucleus and
FT                   cytoplasmic"
FT                   /db_xref="taxon:4896"
FT   CDS             <1..>465
FT                   /codon_start=1
FT                   /product="Hypothetical protein"
FT                   /note="Similar to PUR3_YEAST"
FT                   /db_xref="GENEDB:SPCC569.08c"
FT                   /db_xref="GOA:Q9UUK7"
FT                   /db_xref="InterPro:IPR001555"
FT                   /db_xref="InterPro:IPR002376"
FT                   /db_xref="InterPro:IPR004607"
FT                   /db_xref="UniProtKB/Swiss-Prot:Q9UUK7"
FT                   /protein_id="BAA87143.1"
FT                   /translation="LPPATSYKMVASLVVLISGSGSNLQAIIDATLNGVLKGEAAVTHV
FT                   LSNRKNAYGLERAAKAGIPTSLHTLLPYKKEYGPEIGRKKYDAELAEKIIKLQPSLVVC
FT                   AGWMHILSPEVLIPLETNKIGIINLHPALPGAFNGIHAIERAFEAAQQGKI"
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SQ   Sequence 465 BP; 139 A; 97 C; 105 G; 124 T; 0 other;
     ctaccaccgg ccacttcgta caagatggta gcttcacttg tcgtgttaat ttcgggatca        60
     ggttctaacc tccaagcaat aattgatgct acgctgaatg gagttttaaa gggagaagca       120
     gctgtaacgc acgttttgtc aaaccgcaaa aatgcttatg gcttagaaag agccgcgaaa       180
     gctgggatac caacgtcttt gcatacattg ttaccctaca aaaaggaata cggacctgag       240
     ataggaagaa aaaagtatga tgctgagttg gcggaaaaga taattaagct tcaaccatcc       300
     ttggttgtct gtgctggatg gatgcacatt ttgtccccag aagtgttgat tcctttagaa       360
     acaaataaaa tcggaattat taatcttcat ccagctcttc ctggcgcttt taacggtatt       420
     catgctatcg aacgcgcgtt cgaagctgcc cagcaaggaa agatc                       465
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