spacer
spacer
EBIDatabasesDatabase BrowsingDbfetch

EBI Dbfetch

ID   AB027831; SV 1; linear; genomic DNA; STD; FUN; 191 BP.
XX
AC   AB027831;
XX
DT   30-NOV-1999 (Rel. 61, Created)
DT   21-JUN-2001 (Rel. 68, Last updated, Version 4)
XX
DE   Schizosaccharomyces pombe gene for Hypothetical protein, partial cds,
DE   clone:V125.
XX
KW   .
XX
OS   Schizosaccharomyces pombe (fission yeast)
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Taphrinomycotina;
OC   Schizosaccharomycetes; Schizosaccharomycetales; Schizosaccharomycetaceae;
OC   Schizosaccharomyces.
XX
RN   [1]
RP   1-191
RA   Ding D., Tomita Y., Hiraoka Y.;
RT   ;
RL   Submitted (24-MAY-1999) to the EMBL/GenBank/DDBJ databases.
RL   Da-Qiao Ding, Communications Research Laboratory, Kansai Advanced Research
RL   Center; 588-2, Iwaoka, Iwaoka-cho, Nishiku, Kobe, Hyogo 651-2401, Japan
RL   (E-mail:ding@crl.go.jp, Tel:+81-78-969-2240, Fax:+81-78-969-2249)
XX
RN   [2]
RX   DOI; 10.1046/j.1365-2443.2000.00317.x
RX   PUBMED; 10759889.
RA   Ding D.Q., Tomita Y., Yamamoto A., Chikashige Y., Haraguchi T., Hiraoka Y.;
RT   "Large-scale screening of intracellular protein localization in living
RT   fission yeast cells by the use of a GFP-fusion genomic DNA library";
RL   Genes Cells 5(3):169-190(2000).
XX
CC   An S. pombe gene library in which genomic DNA fragments are fused
CC   to the 5'-end of the GFP-S65T gene was constructed. S. pombe
CC   strain was transformed with the library DNA amplified in E.coli.
CC   Plasmids of those transformants that showed interesting GFP
CC   localization were isolated, and their nucleotide sequences were
CC   determined. Detailed information and images can be seen in our web
CC   site (http://www-karc.crl.go.jp/bio/CellMagic/). Note: Only major
CC   localization is described for each clone in most cases. Because it
CC   is a random fusion of DNA fragment with the GFP gene, the
CC   subcellular localization of the fusion protein may differ from
CC   that of the full-length gene product.
XX
FH   Key             Location/Qualifiers
FH
FT   source          1..191
FT                   /organism="Schizosaccharomyces pombe"
FT                   /strain="968 h90"
FT                   /mol_type="genomic DNA"
FT                   /clone="V125"
FT                   /note="subcellular localization of GFP fusion; Nucleus"
FT                   /db_xref="taxon:4896"
FT   CDS             <1..>191
FT                   /codon_start=1
FT                   /product="Hypothetical protein"
FT                   /db_xref="GENEDB:SPAC25B8.16"
FT                   /db_xref="GOA:Q9UTA4"
FT                   /db_xref="InterPro:IPR009723"
FT                   /db_xref="InterPro:IPR012590"
FT                   /db_xref="UniProtKB/Swiss-Prot:Q9UTA4"
FT                   /protein_id="BAA87135.1"
FT                   /translation="FYEMKRSTGGTQPKGLNVKRSKLADARFIEVESPALSNGAVDLKK
FT                   FIESRSFEITALQDAMKRS"
XX
SQ   Sequence 191 BP; 69 A; 32 C; 41 G; 49 T; 0 other;
     ttttatgaaa tgaagcgttc aactggagga acgcaaccaa agggactaaa cgtcaaaagg        60
     tcaaagctcg ctgatgctag attcatagag gttgaatcac ctgccctttc aaatggtgct       120
     gtagacttaa aaaaattcat tgaatcaagg agttttgaaa taacggcttt acaagatgct       180
     atgaaaagat c                                                            191
//
spacer
spacer