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EBI DbfetchID AB024740; SV 1; linear; genomic DNA; STD; PRO; 3780 BP. XX AC AB024740; XX DT 16-DEC-1999 (Rel. 62, Created) DT 14-APR-2005 (Rel. 83, Last updated, Version 3) XX DE Pyrococcus kodakaraensis Pk-chiA gene for chitinase, complete cds. XX KW chitinase. XX OS Thermococcus kodakarensis KOD1 OC Archaea; Euryarchaeota; Thermococci; Thermococcales; Thermococcaceae; OC Thermococcus. XX RN [1] RP 1-3780 RA Fujiwara S., Tanaka T., Imanaka T.; RT ; RL Submitted (10-MAR-1999) to the EMBL/GenBank/DDBJ databases. RL Shinsuke Fujiwara, Osaka University, Department of Biotecnology; 2-1 RL Yamadaoka, Suita, Osaka 565-0871, Japan RL (E-mail:s.fujiwara@cell.bio.eng.osaka-u.ac.jp, Tel:81-6-877-5111(ex.3445), RL Fax:81-6-879-7441) XX RN [2] RX PUBMED; 10583986. RA Tanaka T., Fujiwara S., Nishikori S., Fukui T., Takagi M., Imanaka T.; RT "A unique chitinase with dual active sites and triple substrate binding RT sites from the hyperthermophilic archaeon Pyrococcus kodakaraensis KOD1"; RL Appl. Environ. Microbiol. 65(12):5338-5344(1999). XX RN [3] RX DOI; 10.1074/jbc.M105919200 RX PUBMED; 11468293. RA Tanaka T., Fukui T., Imanaka T.; RT "Different cleavage specificities of the dual catalytic domains in RT chitinase from the hyperthermophilic archaeon Thermococcus kodakaraensis RT KOD1"; RL J. Biol. Chem. 276(38):35629-35635(2001). XX FH Key Location/Qualifiers FH FT source 1..3780 FT /organism="Thermococcus kodakarensis KOD1" FT /mol_type="genomic DNA" FT /db_xref="taxon:69014" FT RBS 67..72 FT CDS 79..3726 FT /codon_start=1 FT /transl_table=11 FT /gene="Pk-chiA" FT /product="chitinase" FT /db_xref="GOA:Q9UWR7" FT /db_xref="HSSP:1ITX" FT /db_xref="InterPro:IPR012291" FT /db_xref="UniProtKB/TrEMBL:Q9UWR7" FT /protein_id="BAA88380.1" FT /translation="MKKIWTSIVLTAVLLLSLVQVGTFPWASAAESVSLSGSAIAWDVV FT NLTWSPYSSAKAYEVYRSTDPSNLFSPNNLLVVVNWSSYPKYEPGKTYNQGDVVEYNGK FT IWRVKYWTQSVPGSDDSWELVGDVVPTTSYLDQYHLKANTTYYYGVVPVLADGSRGSPS FT NVLAITTPLEPYRVIVYYISWGRYARKFYVSDIPWEKVTHVNYAFLDLKEDGTVAFYDT FT YADPLNLEAMKEYKRKYPAVKVLISVGGWTLSKYFSVVAADPAKRQRFAETAIEILRKY FT NLDGIDIDWEYPGGGGMAGNYESPDDGKNFVLLLKDLREALDKAAKEDHKDYLLTAATP FT ADPVKAGRIDWVEASKYLDSINIMTYDYHGAWETITGHLAPLYCDPNAPYTDENVKYHF FT CVNYTVQWYIQHVPDKTKITVGLPFYSRSFANVPPENNGLYQPFSGTPAGTWGPAYETY FT GVMDYWDVAEKNQSSEYEYHWDPIAQVAWLYSPSKRIFITFDDPRAIGIKVDYMLKNGL FT GGVMIWEITADRKPGTNDHPLLDTVLQHLGEKPPAWIPDTYYIGSNIPSNITVPEPTPL FT PPSNETTPEDNQTNPNPSQGNETNPNPSPGNETTPSDNQTTPSTGDFVKPGSLSVKVTD FT WGNTEYDVTLNLGGTYDWVVKVKLKDGSSVSSFWSANKAEEGGYVVFTPVSWNRGPTAT FT FGFIATGSESVEAIYLYVDGQLWDAWPSNTQQPEENQTVPSPSPGNETTPTPSPGNETA FT PSENQTTPSTGDLVKPDAFSVKIQDWGSTEYDVTLNLGGTYDWVVKVKLKDGSAVSSVW FT SANKAEEGGYVVFTPVSWNKGPTATFGFIATGSEPVEAMYLYVNDQLWDVWPETASAPG FT NESTPSDNQTNPNPSPGNETNPNPSPGNETGPYVPAGPGLPEHFFAPYIDMSLGIHKPL FT VEYYNLTGTPYFTLAFVLYSSVYNGPAWAGSIPLDAFVDEVKGLREAGGDVIIAFGGAV FT GPYLCQQAKTPEQLAQWYIQVIDTYNATYLDFDIESGVDADKLADALLIVQRERPNVRF FT SFTLPSDPGIGLAGGYGIIETMAKKGVIVDRVNPMTMDYYWTPANADNAISVAEHVFNQ FT LKQIYPDKSDDEIWGMIGLTPMIGTNDDKSVFSLQDAEKLVDWAIQHKIRSLAFWSVDR FT DHPGPTGEVSPIHRGTSDPDWAFSHAFLRFMKAFQPVASTAQVAVAVPV" XX SQ Sequence 3780 BP; 976 A; 1127 C; 1003 G; 674 T; 0 other; aaaacattta taagcatgga agttcatgta aaatatgcaa catctcccaa aacattcaaa 60 gctcaagggg tgttgtgtgt gaagaagatt tggacttcaa tagttttgac cgcagtgctc 120 ctgctgtccc tggtccaggt gggcactttc ccctgggctt cagcggcgga gagcgtaagc 180 ctgagcggga gcgctatagc atgggacgtt gtgaacctga cttggagccc gtacagcagc 240 gccaaggcgt acgaggtgta caggagcacg gatccctcca acctgttctc accgaacaac 300 ctgctggtgg tcgttaactg gagcagctat ccgaaatatg agccagggaa gacatacaac 360 cagggcgatg tcgttgagta caacgggaag atatggcgcg ttaagtactg gacccagagc 420 gttccaggaa gcgacgactc ctgggagctc gtaggggatg ttgtcccaac cacaagctac 480 ctcgaccagt atcacctgaa ggcaaacaca acctactact acggggtcgt cccggttctc 540 gccgatggaa gcaggggaag tccctcgaac gtcctcgcca taacgactcc cctcgaacct 600 tacagggtca tagtctacta catctcctgg ggaaggtacg cgaggaagtt ctacgtgagc 660 gacatcccct gggagaaggt tacccacgtc aactacgcct tcctcgacct caaggaggat 720 ggaaccgttg ccttctacga tacctacgct gatcctctca acctcgaggc catgaaggag 780 tacaaaagga agtatccagc cgtcaaggtt ctcatctcag tcggcggatg gactctcagc 840 aagtacttct cggtggtcgc cgcagacccg gccaagagac agcgcttcgc cgagactgcc 900 atagagatcc tcagaaagta caacctcgac ggaattgata tcgactggga gtacccgggc 960 ggcggaggta tggcgggcaa ctacgagagt cccgacgacg gtaagaactt cgttcttctc 1020 ctcaaggatt tgagggaggc cctcgacaag gccgcaaaag aggaccacaa ggactacctc 1080 ctaacggccg caacgccggc cgatccggtt aaggctggca ggatagattg ggtggaggcg 1140 agcaagtacc tcgattcaat caacatcatg acctacgact accacggcgc ctgggagacc 1200 ataaccggcc accttgcccc gctctactgc gatccaaacg cgccatacac cgatgagaac 1260 gtcaagtacc acttctgcgt caactacacc gtccagtggt acatccagca cgttcccgat 1320 aagaccaaga taaccgtcgg cctgcccttc tacagcagga gctttgccaa cgtcccgccc 1380 gagaacaacg gcctctacca gcccttcagc ggcaccccag ctggaacctg gggaccggcc 1440 tacgagacct acggagttat ggactactgg gacgttgccg agaagaacca gagcagcgag 1500 tacgagtacc actgggatcc gatagcccag gtggcctggc tctactcccc gagcaagagg 1560 atattcataa ccttcgacga tcccagggcg atcgggataa aggttgacta catgctgaag 1620 aacggcctcg gcggagtgat gatatgggag atcacagctg acaggaagcc cgggaccaac 1680 gaccacccgc ttctcgatac tgttctccag cacctcggcg agaagccgcc ggcgtggatt 1740 ccggacacct actacatcgg ctcgaacatc ccgagcaaca taaccgttcc agagccgacg 1800 ccactgccgc cgagcaacga gacaacccct gaggacaatc agaccaatcc aaacccatca 1860 cagggaaatg aaaccaatcc aaacccgtca cctggaaacg agaccacacc ctcggacaac 1920 cagacgactc catccaccgg ggattttgtc aagccgggtt ctttgagcgt caaggtaacc 1980 gactggggca acactgaata cgatgtcacc ctcaacctcg gtggaaccta tgactgggtc 2040 gtcaaggtca aactcaagga cggttcaagc gtatcgagct tctggagcgc gaacaaggcc 2100 gaggaaggcg gttacgtcgt cttcacgccg gtgagctgga acagggggcc gacggcaacg 2160 ttcggattca tcgccactgg aagcgagtcc gttgaagcga tttacctcta cgtagacggc 2220 cagctctggg atgcctggcc ctcaaacacc caacaaccgg aggaaaacca gacagtccca 2280 agcccgtcac ctggcaacga gacgactccc acaccctccc ccggaaacga gaccgcaccc 2340 tcagagaacc agacaactcc gtccacggga gatctagtca agccggatgc attcagcgtt 2400 aaaatccagg actggggaag cacggagtac gatgtaaccc tgaacctcgg tggaacttac 2460 gactgggtcg ttaaagtcaa gctcaaggac ggatcagccg tctcaagcgt ctggagcgct 2520 aacaaggccg aggaaggtgg ttacgtagtc ttcactcccg taagctggaa caagggaccg 2580 acggcaacct tcggcttcat cgccactggc agcgagcccg ttgaggccat gtacctctac 2640 gtaaacgatc agctctggga cgtctggcca gaaaccgcat cagccccggg caacgagagt 2700 actccatccg acaaccagac caatccgaat ccatcaccgg gcaacgaaac caatccaaat 2760 cccagcccag gaaacgagac gggcccgtac gttccagcag gcccaggtct tcccgagcac 2820 ttcttcgccc catacatcga catgagcctc ggcatacaca agccgctcgt tgagtactac 2880 aacctcacgg gaacaccgta cttcacactc gccttcgtcc tctacagttc agtctacaac 2940 gggccggcct gggctggaag cattcccttg gatgccttcg ttgatgaggt aaaagggctc 3000 agggaagctg ggggagacgt tattatagcc ttcgggggcg cagttggccc ctacctctgc 3060 cagcaggcaa aaactccaga acagctcgcc cagtggtaca ttcaggtcat agacacctac 3120 aacgccacat acctcgattt tgacatcgaa tctggtgtag acgccgacaa acttgcggat 3180 gctctcctga tcgtccagag ggagaggccg aacgtcaggt tcagcttcac actcccgagc 3240 gacccgggaa taggtctcgc gggcggatac ggaataatag agaccatggc aaagaagggg 3300 gtcattgtcg acagggtcaa tccgatgacg atggactact actggacgcc agcaaacgcc 3360 gacaacgcca taagcgtggc cgaacacgtc tttaaccagc tcaagcagat ctatcctgac 3420 aagagcgacg acgaaatatg gggcatgatc ggcctgactc cgatgatagg aaccaacgac 3480 gacaagagcg tcttctcgct ccaggatgcc gaaaagctcg tcgactgggc aattcagcac 3540 aagatacgct cccttgcctt ctggagcgtt gacagagatc acccagggcc gacaggagag 3600 gtctcaccaa tccacagggg aaccagcgac cccgattggg ccttcagcca cgccttcctg 3660 aggttcatga aggcattcca gccggttgcc agcacggctc aggtggcagt tgcagttcca 3720 gtttgacccc tctcttctcc tcttttgtcc ataatgaaat tggagaaaca gctaaggaag 3780 // |
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