EBI Dbfetch

ID   AB016777; SV 1; linear; genomic DNA; STD; PRO; 1788 BP.
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AC   AB016777; D16315;
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DT   12-AUG-1998 (Rel. 56, Created)
DT   09-JUN-2005 (Rel. 84, Last updated, Version 4)
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DE   Ruminococcus albus gene for endo-1,4-beta-glucanase, complete cds.
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KW   endo-1,4-beta-glucanase.
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OS   Ruminococcus albus
OC   Bacteria; Firmicutes; Clostridia; Clostridiales; Ruminococcaceae;
OC   Ruminococcus.
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RN   [1]
RP   1-1788
RA   Karita S.;
RT   ;
RL   Submitted (10-AUG-1998) to the EMBL/GenBank/DDBJ databases.
RL   Shuichi Karita, Mie University, Faculty of Bioresouces, Department of
RL   Sustainable Resource Science; 1577 Kurimamachiya, Tsu, Mie, 514-8507, Japan
RL   (E-mail:karita@bio.mie-u.ac.jp, Tel:81-59-231-9619, Fax:81-59-231-9604)
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RN   [2]
RA   Karita S., Kimura T., Sakka K., Ohmiya K.;
RT   "Purification of the Ruminococcus albus endoglucanase IV using a
RT   cellulose-binding domain as an affinity tag";
RL   J. Ferment. Bioeng. 84:354-357(1997).
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RN   [3]
RA   Karita S., Sakka K., Ohmiya K.;
RT   "Cellulose-binding domains confer an enhanced activity against insoluble
RT   cellulose to Ruminococcus albus endoglucanase IV";
RL   J. Ferment. Bioeng. 81:553-556(1996).
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RN   [4]
RA   Karita S., Morioka K., Kajino T., Sakka K., Shimada K., Ohmiya K.;
RT   "Cloning and sequencing of a novel endo-1,4-beta-glucanase gene from
RT   Ruminococcus albus";
RL   J. Ferment. Bioeng. 76:439-444(1993).
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FH   Key             Location/Qualifiers
FH
FT   source          1..1788
FT                   /organism="Ruminococcus albus"
FT                   /strain="F-40"
FT                   /mol_type="genomic DNA"
FT                   /db_xref="taxon:1264"
FT   CDS             305..1243
FT                   /codon_start=1
FT                   /transl_table=11
FT                   /product="endo-1,4-beta-glucanase"
FT                   /note="Swiss-Prot Acc: Q07940"
FT                   /note="a member of glycosyl hydrolase family 5"
FT                   /db_xref="GOA:Q07940"
FT                   /db_xref="InterPro:IPR001547"
FT                   /db_xref="InterPro:IPR013781"
FT                   /db_xref="InterPro:IPR017853"
FT                   /db_xref="InterPro:IPR018087"
FT                   /db_xref="UniProtKB/Swiss-Prot:Q07940"
FT                   /protein_id="BAA32286.1"
FT                   /translation="MLDKLKVINGKLTAGEKPVRLFGLSTHGIAWYPEYICEESFNALK
FT                   KDWRTNCIRIAMYTDEFRGYCKDGNKQHLKELIEKGVVIAEKLDMYVIVDWHVLCDQDP
FT                   MKYIDEAEEFFSDMSKRFANKTNVIYEICNEPNCSGTWDKITEYADRIIPIIRSNSPDA
FT                   LIVTGTSTWSQDIHCALEKPLKWDNVMYSLHFYAATHKGTLRSRLERCIEAGLPVFINE
FT                   FNLCEASGKGDIDIDEANAWYEVIDRLGLSCISWCLSNSGDTCGVFTQNCTKLSGWTDE
FT                   DIKTSGKIIKGWFEAFADEENTNEQCFRIDK"
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SQ   Sequence 1788 BP; 589 A; 329 C; 408 G; 462 T; 0 other;
     ctgcagtata ttatgaactc ccccatcatt gaacgaaatg atatatcatt agtttctgaa        60
     agctgttcca gaacataatt caggtattcc ttgcttgaag acattacctc atccctcccg       120
     ttttttatct gaatgtcaat taatatctgt tatacgatta taccataaaa gaaaataata       180
     agcaactgtc catggtcata ttaaatttat attttttgta gaaaaaatcc atattattac       240
     tttataatta aaatggaaat atataggata atgtagcatt aggcactcaa aaggagctga       300
     tactatgttg gataaactta aagtaataaa tggaaaactg acagccggtg agaaaccagt       360
     aagacttttc gggttatcaa ctcacggtat agcctggtat ccggaatata tttgtgaaga       420
     atcattcaat gccctaaaaa aagactggcg tacaaactgt ataaggatag caatgtacac       480
     agacgaattc agagggtact gcaaggacgg caacaaacag catctgaaag agctgatcga       540
     gaaaggcgtt gttattgcag aaaaactgga tatgtatgta atagtagact ggcacgttct       600
     gtgtgatcag gatccgatga aatatattga tgaagctgaa gagtttttca gtgatatgtc       660
     aaagaggttc gccaataaaa caaacgttat atacgagata tgtaacgaac ctaactgcag       720
     cggcacatgg gataaaataa cagaatatgc agacaggata atcccgataa tccgcagtaa       780
     ctcccccgat gcacttattg tcacaggaac atcaacgtgg tcgcaggaca tacactgtgc       840
     gcttgaaaag ccgctgaaat gggacaatgt tatgtactct ctgcatttct atgctgctac       900
     acacaagggt acactgcgca gcagactgga acgatgtatt gaagccgggc ttccggtttt       960
     tatcaatgaa ttcaatctgt gtgaagctag cggtaagggc gatatcgata tagatgaagc      1020
     aaatgcatgg tatgaggtaa tagacagact cgggctgagc tgcataagct ggtgcctttc      1080
     aaacagtgga gatacctgcg gcgtttttac ccaaaattgt acaaagcttt caggctggac      1140
     ggatgaagat ataaaaacat cgggcaaaat aataaaaggc tggtttgagg catttgcaga      1200
     tgaggagaat acaaatgaac aatgttttcg aattgataaa tgagcgtaaa agcgtcagga      1260
     cctttgacgg cagtgaaata acaccttcag tgcgagaaga tatactcagt tacgcacata      1320
     gtataaaaac cccgtacgaa caggatatag agatacgtat tctggataat ggtgtacatg      1380
     gtctgtcaag ccctgttatt gttggtactg acacttatat cggcggaaag atgaagcgtg      1440
     ctgatcatgc agaggaagct ttcggttact attttgaaca gcttgtgctc tacatccaat      1500
     cactggggct gggtactaca tggataggcg gcactatgga cagaaaagca tttgaggatg      1560
     ctatggagat tgcagacgga gaggtaatgc cctgcgttac tcctataggt acccctgcgg      1620
     ataaacgctc actcagagag ataatgatgc gcaaaggcat caaagctgac agtcgtaaaa      1680
     attttgagga gctctttttt gacggaagtg ccggcacacc actcagtgaa ggcagatatc      1740
     ccgaactcac cgagatactg gaagcagtaa ggcttgcacc ctcagctg                   1788
//