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EBI DbfetchID AB015592; SV 1; linear; genomic DNA; STD; PRO; 2815 BP. XX AC AB015592; XX DT 09-JUL-1998 (Rel. 56, Created) DT 14-APR-2005 (Rel. 83, Last updated, Version 3) XX DE Bacillus subtilis gene for alpha-amylase precursor, complete cds. XX KW alpha-amylase precursor. XX OS Bacillus subtilis OC Bacteria; Firmicutes; Bacillales; Bacillaceae; Bacillus. XX RN [1] RP 1-2815 RA Ohdan K., Kuriki T.; RT ; RL Submitted (12-JUN-1998) to the EMBL/GenBank/DDBJ databases. RL Kohji Ohdan, Ezaki Glico co., Ltd., Biochmical Research Laboratory; Utajima RL 4-6-5, Nishiyodogawa-ku, Osaka, Osaka 555-8502, Japan RL (E-mail:odan-kouji@glico.co.jp, Tel:+81-6-477-8425(ex.2763), RL Fax:+81-6-477-8271) XX RN [2] RX PUBMED; 10508102. RA Ohdan K., Kuriki T., Kaneko H., Shimada J., Takada T., Fujimoto Z., RA Mizuno H., Okada S.; RT "Characteristics of two forms of alpha-amylases and structural RT implication"; RL Appl. Environ. Microbiol. 65(10):4652-4658(1999). XX FH Key Location/Qualifiers FH FT source 1..2815 FT /organism="Bacillus subtilis" FT /mol_type="genomic DNA" FT /db_xref="taxon:1423" FT RBS 535..540 FT CDS 549..2528 FT /codon_start=1 FT /transl_table=11 FT /product="alpha-amylase precursor" FT /EC_number="3.2.1.1" FT /db_xref="GOA:O82953" FT /db_xref="HSSP:1BAG" FT /db_xref="InterPro:IPR013781" FT /db_xref="UniProtKB/TrEMBL:O82953" FT /protein_id="BAA31528.1" FT /translation="MFEKRFKTSLLPLFAGFLLLFHLVLSGPAAANAETANKSNKVTAS FT SVKNGTILHAWNWSFNTLTQNMKDIRDAGYAAIQTSPINQVKEGNQGDKSMSNWYWLYQ FT PTSYQIGNRYLGTEQEFKDMCAAAEKYGVKVIVDAVVNHTTSDYGAISDEIKRIPNWTH FT GNTQIKNWSDRWDITQNALLGLYDWNTQNTEVQAYLKGFLERALNDGADGFRYDAAKHI FT ELPDDGNYGSQFWPNITNTSAEFQYGEILQDSASRDTAYANYMNVTASNYGHSIRSALK FT NRILSVSNISHYASDVSADKLVTWVESHDTYANDDEESTWMSDDDIRLGWAVIGSRSGS FT TPLFFSRPEGGGNGVRFPGKSQIGDRGSALFKDQAITAVNQFHNEMAGQPEELSNPNGN FT NQIFMNQRGSKGVVLANAGSSSVTINTSTKLPDGRYDNRAGAGSFQVANGKLTGTINAR FT SAAVLYPDDIGNAPHVFLENYQTEAVHSFNDQLTVTLRANAKTTKAVYQINNGQETAFK FT DGDRLTIGKEDPIGTTYNVKLTGTNGEGASRTQEYTFVKKDPSQTNIIGYQNPDHWGNV FT NAYIYKHDGGGAIELTGSWPGKAMTKNADGIYTLTLPANADTADAKVIFNNGSAQVPGQ FT NHPGFDYVQNGLYNNSGLNGYLPH" FT sig_peptide 549..683 FT /experiment="experimental evidence, no additional details FT recorded" FT terminator 2603..2652 XX SQ Sequence 2815 BP; 858 A; 570 C; 681 G; 706 T; 0 other; aagcttggcg gttttcttca ttataattgt ttattattgc atcagggcgg gagcgtcggg 60 aatgctcatg ccgagaatag acaccaaaga agaactgcaa aagcgggtga agcagcagcg 120 gatagattca attgcggtca cttttgcggt agcggcgatt acgatgtacg acagggggtt 180 tccccataca ttctttgctt ggctgaaaat gatctttctt tttatcgtct gcggaggcat 240 tctgtttctg cttcggtatg tcattgtgaa gctgtcttac agaagagcga tgaaagaaat 300 aaaaaagaaa tcatcttgac aggtggtttc tttttttgtt tgaaagcggg aagtcctttt 360 aattgatagg aatatagatt tgaattcact ctgtcacgtt gctttgatag agagagtgtg 420 ataagttgaa atgtaagcgt taacaaaatt atccagtctt cacaacagtt gaaaggagct 480 gaagaaagag taagaggaat ttttgactcc gcagtcagtc ttcaaaaatg aaataaggag 540 tgtcaaaaat gtttgaaaaa cgattcaaaa cctctttact gccgttattc gccggatttt 600 tattgctgtt tcatttggtt ttgtcaggcc cggcggctgc aaacgctgaa actgcaaaca 660 aatcgaataa ggtgaccgcg tcatcggtca aaaacgggac catccttcat gcatggaatt 720 ggtcgttcaa tacgttaaca caaaatatga aagatattcg tgatgcgggc tatgcagcca 780 ttcagacgtc gccgattaac caagtaaagg aagggaacca aggagataaa agcatgtcga 840 actggtactg gctctatcag ccgacatcgt accaaatcgg caaccgttac ttaggcactg 900 aacaagaatt taaggacatg tgtgcagccg cggaaaagta tggcgtaaaa gtcattgtcg 960 atgcggttgt caatcatacc accagcgatt atggcgcgat ttctgatgag attaagcgta 1020 ttccaaactg gacccatgga aacacacaaa ttaaaaattg gtcggaccga tgggacatca 1080 ctcaaaatgc attgcttggg ctgtatgatt ggaatactca gaatactgag gtgcaagcct 1140 acctgaaagg tttcttggaa agagcattga atgacggagc agacgggttc cgctatgatg 1200 ccgccaagca tatagagctt ccggatgatg ggaattacgg cagccaattt tggccgaata 1260 tcacaaatac atcggcggag ttccaatacg gagaaatcct gcaagacagc gcgtccagag 1320 atactgctta tgcgaattat atgaatgtga cggcttctaa ctatgggcat tccatcagat 1380 ccgctttaaa gaatcgtatt ctgagtgtgt cgaatatctc ccattatgca tctgatgtgt 1440 ctgcggacaa gttagtcaca tgggtggaat cacatgatac gtatgccaat gatgatgaag 1500 agtccacatg gatgagtgat gacgatattc gtttaggctg ggcagtgatt ggttcccgct 1560 caggaagcac gcctcttttc ttttccagac ctgagggcgg aggaaatggt gtaagatttc 1620 ccggaaaaag tcaaatagga gatcgcggga gcgccttatt taaagaccag gcgatcactg 1680 cggtcaatca atttcacaat gaaatggccg ggcagcctga ggaactctca aatccgaatg 1740 gcaacaatca aatatttatg aatcagcgcg gctcaaaagg cgttgtgctg gcaaatgcag 1800 gatcgtcttc tgtcaccatc aatacttcaa cgaaattacc tgacggcagg tatgataata 1860 gggccggcgc cggttcattt caagtagcga acggcaaact gacaggtacg atcaatgcca 1920 gatccgcggc tgttctttat cctgatgata ttggaaatgc gcctcatgtc tttcttgaga 1980 attaccaaac agaggcagtc cattctttca atgatcagct gacggtcacc ctgcgtgcaa 2040 atgcgaaaac aacaaaagcc gtttaccaaa tcaataatgg gcaggagaca gcatttaagg 2100 atggagaccg attaacgatc gggaaagaag atccaatcgg gacgacatac aacgtcaagt 2160 taaccggaac gaacggcgag ggtgcatcga gaacccaaga atacacgttt gtcaaaaaag 2220 acccgtccca aaccaacatc attggctatc aaaatccgga tcattggggc aatgtaaatg 2280 cttatatcta taaacatgat ggaggcgggg ccatagaatt aaccggatcg tggccgggga 2340 aagccatgac taagaatgca gatggaattt acacgctgac gctgcctgcg aatgcggata 2400 cggccgacgc caaagtgatt tttaacaatg gcagcgccca agtgcccgga cagaaccatc 2460 ccggctttga ttatgtgcag aatggtttgt ataacaactc tggtttgaat ggttatcttc 2520 cgcattaatg gatagggcca tcattacatc aaacccttgc ttcggttagg aaagggacag 2580 cgcgtcttca atcacgctga aaagatgaca acatcctaaa acggttccgt tttaggatgt 2640 tgtcaacaat ctggaaacca tcaccggtat tttttgtcca aaaatcaaac ttttctcttg 2700 cgaaagtttg tgaagtgttg cacaatataa atgtgaaata cttcacaaga taaaaacaga 2760 caccacgaaa cactggagga tgagcataca tgatgaacga acgagtgaac aagtt 2815 // |
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