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EBI DbfetchID AB012238; SV 1; linear; genomic DNA; STD; PRO; 3854 BP. XX AC AB012238; XX DT 01-APR-1999 (Rel. 59, Created) DT 31-AUG-2006 (Rel. 89, Last updated, Version 4) XX DE Bacillus thermoamyloliquefaciens gene for alpha-glucosidase, complete cds. XX KW alpha-glucosidase. XX OS Bacillus thermoamyloliquefaciens OC Bacteria; Firmicutes; Bacillales; Bacillaceae; Bacillus. XX RN [1] RP 1-3854 RA Kashiwabara S.; RT ; RL Submitted (17-MAR-1998) to the EMBL/GenBank/DDBJ databases. RL Shin-ichi Kashiwabara, University of Tsukuba, Institute of Applied RL Biochemisty; Tsukuba, Ibaraki 305-8572, Japan RL (E-mail:kashiwa@sakura.cc.tsukuba..ac.jp, Tel:0298-53-6596, RL Fax:0298-53-6278) XX RN [2] RX DOI; 10.1271/bbb.64.1379 RX PUBMED; 10945254. RA Kashiwabara S., Azuma S., Tsuduki M., Suzuki Y.; RT "The primary structure of the subunit in Bacillus thermoamyloliquefaciens RT KP1071 molecular weight 540,000 homohexameric alpha-glucosidase II RT belonging to the glycosyl hydrolase family 31"; RL Biosci. Biotechnol. Biochem. 64(7):1379-1393(2000). XX FH Key Location/Qualifiers FH FT source 1..3854 FT /organism="Bacillus thermoamyloliquefaciens" FT /strain="KP1071" FT /mol_type="genomic DNA" FT /db_xref="taxon:1425" FT -35_signal 358..363 FT -10_signal 388..395 FT RBS 425..429 FT CDS 438..2801 FT /codon_start=1 FT /transl_table=11 FT /product="alpha-glucosidase" FT /EC_number="3.2.1.20" FT /note="catalytic residue(Asp):nucleotide position from1219 FT to1221, amino acid position at 407" FT /db_xref="GOA:Q9F234" FT /db_xref="InterPro:IPR000322" FT /db_xref="InterPro:IPR017853" FT /db_xref="UniProtKB/Swiss-Prot:Q9F234" FT /protein_id="BAA76396.1" FT /translation="MLEDTSFAIQPEQDDKTQETHRIDIGNMHTFSHTEHVFSFHCDTG FT IVKIRFYREDIVRIAFNPFGETSLSTSVAVVKEPEKVDASVHETEEEVTLTSAKQTVVL FT QKRPFRVRIYDNHGRLLVAEGKKGMAFTYQGEVCCFKMMDEADHFYGFGEKTGFLDKRG FT ETMTMWNTDVYAPHNPETDPLYQSHPYFMTVRNGSAHGIFFDNTYKTTFDFQTATDEYC FT FSAEGGAIDYYVFAGPTPKDVLEQYTDLTGRMPLPPKWALGYHQSRYSYETEQEVREIA FT QTFIEKDIPLDVIYLDIHYMNGYRVFTFDRNRFPNLKQLIADLKQKGIRVVPIVDPGVK FT EDPEYVIYQEGIRHDYFCKYIEGNVYFGEVWPGKSAFPDFTNKKVRKWWGEKHQFYTDL FT GIEGIWNDMNEPSVFNETKTMDVKVIHDNDGDPKTHRELHNVYGFMMGEATYKGMKKLL FT NGKRPFLLTRAGFSGIQRYAAVWTGDNRSFWEHLQMSLPMCMNLGLSGVAFCGPDVGGF FT AHNTNGELLTRWMQVGAFTPYFRNHCAIGFRRQEPWAFGEKYERIIKKYIRLRYQWLPH FT LYTLFAEAHETGAPVMRPLFFEYPDDENTYNLYDEFLVGANVLIAPIMTPSTTRRVAYF FT PKGNWVDYWTGEVLEGGQYHLISADLETLPIFIKQGSAIALGDVKRSTEMPDEHRTVHI FT YKANGGKATYVLYDDDGQTFSYEKGDYLRMYIEVEYGENSVHIVTKSEGTYQPSWKLSF FT AIHHATEQTKVTIDGNEQNAIFDPHQRILLIQSE" XX SQ Sequence 3854 BP; 1174 A; 800 C; 943 G; 937 T; 0 other; gtactgacaa ggaaatcatt gattatatta aaaaattagg ggaactgcgc caacagctac 60 catcgctacg gcgcggcgat tttacattgt tatatgaaaa aaacggtatg gctgtcttta 120 agcggacgta taaagacgaa acagcggtca ttgccattaa caataccgga aaaacacgaa 180 aggttcattt gacaaacaat caacttgccc cgggaaaaga actacgcgga ctgctggcag 240 gagatttagt ccgaagcgat cgcgacggat atgatctcat tttaaaacgc gagacagcgg 300 aaatttatgt gcttgcgaaa aagacggggc tcaatattcc atttattgca acgttaattg 360 ccatttatgt gctatttgga ttgtttttat attatgtaaa gaaaaggtca aaacagacaa 420 cctaggaggg aaaaaagatg ctcgaggata caagcttcgc cattcagccg gaacaagatg 480 acaaaacgca agaaacgcac cggatcgaca tcgggaatat gcatacattt tctcatacgg 540 aacacgtatt ttcattccat tgcgacacag gaatcgtcaa aattcgattt taccgtgaag 600 atattgtccg tatcgccttc aacccttttg gcgaaacaag tttgtccaca agtgtcgctg 660 tcgtaaaaga gccggaaaaa gtagatgcct ccgtacatga aaccgaggaa gaggtgacgt 720 taacatcggc aaaacagacg gttgttttgc aaaaacgacc gtttcgcgtc cgcatttacg 780 acaatcatgg acgactgctt gttgcggaag gaaaaaaggg gatggcgttt acctaccaag 840 gagaagtatg ctgttttaaa atgatggatg aagctgacca tttttacgga tttggtgaaa 900 aaacagggtt cctcgataag cgtggcgaaa caatgacgat gtggaatacg gatgtgtatg 960 cgccacacaa tccggaaacg gacccgttat accaatccca tccgtatttt atgacggtgc 1020 gaaatggaag tgcgcatgga attttctttg acaatacgta caaaacaaca tttgattttc 1080 aaacagcgac cgatgagtat tgtttttccg cagaaggagg ggccattgat tattacgttt 1140 ttgctggtcc aacgccaaag gacgtgttag aacaatatac ggatcttacc ggccgaatgc 1200 cgcttccgcc aaaatgggca ctcgggtatc atcaatcccg ctatagctat gaaaccgaac 1260 aagaagtaag agagatcgca caaacgttta tcgaaaaaga cattccgctt gatgtcattt 1320 atttagatat tcattacatg aatggctatc gcgtgtttac gtttgaccgt aaccgttttc 1380 cgaatctgaa gcagctcatt gccgatttaa aacaaaaagg aattcgtgtt gttccgattg 1440 tcgaccccgg agtgaaagaa gacccagaat acgtcattta ccaggaaggc attcgccatg 1500 attatttttg caaatatatc gaaggaaacg tttattttgg agaagtctgg ccgggcaaga 1560 gcgcgtttcc ggattttaca aataaaaagg tgcgaaaatg gtggggagaa aagcaccaat 1620 tttatacgga tttaggcatt gaaggcattt ggaacgacat gaacgaacca tccgttttta 1680 acgaaacgaa aacgatggac gtcaaagtga tacacgacaa cgacggcgat ccgaaaacgc 1740 atcgcgagct tcataacgtg tatggcttta tgatgggaga agcaacgtat aaaggaatga 1800 aaaaattatt aaacggaaag cgcccgttct tattaacacg cgctggcttt tccggcattc 1860 agcgctatgc cgcggtatgg acgggggata atcgcagctt ctgggaacac ttgcaaatgt 1920 cactgccgat gtgtatgaac ttaggattat ctggagttgc gttttgcggt ccggatgttg 1980 gcggttttgc ccataacacg aatggagagc tgttgacgcg atggatgcaa gtaggggcgt 2040 ttacgccata ttttcgcaat cattgcgcga ttgggttccg ccgtcaagaa ccgtgggcgt 2100 ttggggaaaa atatgagcgc atcataaaaa aatatattcg attgcgctat cagtggcttc 2160 cacatttata tacattgttt gcggaggcac atgaaacagg ggcacctgtc atgagaccat 2220 tgtttttcga gtacccggat gatgaaaaca cgtataatct gtatgacgaa tttctcgttg 2280 gtgccaatgt attaattgcg ccgatcatga cgccgagcac gacccgtcgc gtcgcttatt 2340 tcccgaaagg aaactgggtg gattattgga caggcgaagt gctagaaggc ggccaatacc 2400 atttaatttc tgcggattta gagacattgc caattttcat taaacaagga tcggctattg 2460 ccttaggtga cgttaagcgt tcgacagaaa tgccggacga acatcggact gttcatattt 2520 ataaggccaa tggtggcaaa gcgacgtatg tactgtatga tgatgacgga caaacgttct 2580 cttacgaaaa gggagattat ttacgcatgt atattgaggt cgaatatggc gaaaattccg 2640 tgcatatcgt aacgaaaagc gaaggaacgt atcaaccaag ttggaagctc tcttttgcaa 2700 ttcatcacgc gacggaacag acgaaagtca cgatcgatgg aaacgaacaa aatgcgatat 2760 ttgatcctca tcagcgtatc ctactcatcc aaagtgaatg agcatcgaat gggggagtaa 2820 atgatgaacg ttacgataaa agatgttgca aaacgagcaa acgtggctcc ctccacggtt 2880 tcccgcgtta ttgccgacag tccgcgaatt agtgagaaga cgaagcgaaa agtgcgacag 2940 gcaatgaaag aacttggcta tcatccgaat tttattgcgc gcagtttggc caatcaagca 3000 acacaggtga tcggcattgt catgccaagc gcagcgggac aggcgctgca aaacccgttc 3060 tttccagaag tcatccgcgg catcagcaaa gcagcacatg aaaaaaaata cgctttgcaa 3120 atgtccacag gcgaaaaaga aaaagaaatt tacgaagggg ttgtggacat gcttcaaggc 3180 cgccgcgtcg acggagtgat tttattatat tcgcgcatca atgatagact gatgaaatat 3240 ttgcaaaaac atcaattccc atttgtcgtc atcgggaagc cgcatcaaaa ggaagaacaa 3300 atcacccatg tcgataatga caattaccgg gcgggcaaag aagcgacgga gcatttgatc 3360 gcgcgcggcc atgaacgaat cgcttttgtc ggcggcaata agcaatattt agtgactgtc 3420 gaccgattgc atggttacga ggaggctttg agagaagcgg gccttcctta tcgcgaagac 3480 tatatcatgc acgaagaatt tttgcaggaa ggcggccaag aagcgatgaa agagctgctt 3540 tcgctcgctg agccgccgac agcgctcgtc gttgccgatg atttaatggc cttaggcgtg 3600 ctgaagacat tggatgaaat gggactgcgc gttcccgatg acatttcgat cgtcagcttc 3660 aacaacacgc tgctggcgga aatgtcgcgc ccgccgttaa cctcggtcga cattcatatc 3720 ttccaactcg gctacgaagc aacgaaaaac ttaatcgaaa aaatcgctaa cccaaatgaa 3780 ccagtcaagc gcattattat tccgcatcgc attgttgagc gtttttcgtg caacgatggc 3840 aggaaataaa gctt 3854 // |
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