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EBI DbfetchID AB006853; SV 1; linear; genomic DNA; STD; PRO; 8333 BP. XX AC AB006853; XX DT 27-JUN-1998 (Rel. 56, Created) DT 14-APR-2005 (Rel. 83, Last updated, Version 3) XX DE Bacillus sp. gene for gellan lyase precursor, complete cds. XX KW gellan lyase precursor. XX OS Bacillus sp. OC Bacteria; Firmicutes; Bacillales; Bacillaceae; Bacillus. XX RN [1] RP 1-8333 RA Hashimoto W., Sato N., Kimura S., Murata K.; RT ; RL Submitted (27-AUG-1997) to the EMBL/GenBank/DDBJ databases. RL Wataru Hashimoto, Kyoto University, Research Institute for Food Science; RL Gokasho, Uji, Kyoto 611, Japan (E-mail:hasimoto@food2.food.kyoto-u.ac.jp, RL Tel:0774-38-3768, Fax:0774-38-3767) XX RN [2] RX DOI; 10.1006/abbi.1998.0674 RX PUBMED; 9633595. RA Hashimoto W., Sato N., Kimura S., Murata K.; RT "Polysaccharide lyase: molecular cloning of gellan lyase gene and formation RT of the lyase from a huge precursor protein in Bacillus sp. GL1"; RL Arch. Biochem. Biophys. 354(1):31-39(1998). XX FH Key Location/Qualifiers FH FT source 1..8333 FT /organism="Bacillus sp." FT /strain="GL1" FT /mol_type="genomic DNA" FT /db_xref="taxon:1409" FT CDS 581..8008 FT /codon_start=1 FT /transl_table=11 FT /product="gellan lyase precursor" FT /db_xref="GOA:O82833" FT /db_xref="HSSP:1K85" FT /db_xref="InterPro:IPR011490" FT /db_xref="UniProtKB/TrEMBL:O82833" FT /protein_id="BAA29068.1" FT /translation="MRFSWKKLVSAALVMALLVGIVYPAASGRGAVASAASGTTVELVP FT TDDAFTSAVAKDANANGTWMQLKGSIGGQRYIYMKFDLTALAGVEADRIENAKVWLKKM FT GTNGTAMTVGLRAVDDTSWSESTLTWNNAPVYGSQVLSQQSVLSTPDVYYPFDLDEYLK FT TQLAAGKSKLAIAFVPISTLNENMEFYARESTANTPKLVVELKDEPPAPTGLMQLVQSF FT GGHNKGHLRVVEFDATPASTTGNGTVGITADGAAPAAAADFPIALRFGTDGTIKAANAA FT GFESKTPVNYTAGQKYHVKAMINLSLGTYDLWLTPPNAGQPVLLAADYAFAASAPALND FT IGGVHATADAQSTDVPAVANARLIADHFVSKAPFKDEQGQSLAIRLESDNSLANRSYAI FT KFDMNLTGNPLETDALISYADRSVTLNGFPDLAYIVRSNFGNFDVRNDNVYASSHPSTA FT QSNRTYQVEVRINPASGTGQPTRTYDVWIAPEGEQPVQLADQFKARNYANTGYALNNIG FT QAFVYSQADGLLSIDNHVVQDGQRLDEALARVNAASGEAAMTAALESNALGLPMERYRL FT MDAAKRAQVAQDVLAGRPAEGYAHALSVQAVFVSAVANRLDTENPTAPANVQVAISNTM FT QAHVSWTASSDDTGILYYKVFRDGALVGTVTNATSFVDNGLAPATEYTYVVKAYDLVLK FT EAVSQPATATSPGEQAQVRIPFSAEAIATAFGQPLLDYNLETHSGTLKWVMEWREEYEK FT SANALKLLTLLSASAPDYIGPDGVTTASAKALQHLRSVTAGGNEPGFAGNGLSGQGYMP FT LLSAIVMAKKKAPAIWNALTAAEKEKLDLMILAGLYGAKFAYDDENDNKTGIDATGNFD FT KEWNPNHRSGIAGAIMAMYYFEDAQWLNDQMRSFNYDDWLARLTAAGLTNVRTIYQNSG FT KTLTEREIRKDAAGDGFVYKGHPLSQPGKIMAEFVNYTFSHPVSPVGGFDSGIGKYRGY FT IVDGQDDLPNLGADSMGFEFDTLDANGKRSSLVYVFMGWKPNVDAITPVLLLDNIDSGL FT TSAETRDVVSRLSIGTTDMLYKNEHGYMTYAKGVNEGVKSLNGPILTINEEIWNRILNN FT PAAPMEAVNQASSAGQMRTALEASALGMILYGYGALSETGKNAVAQHVLDARPAAGYAN FT KAAAQNELYEGVRLQALLALSQAQTAEQMRSALESRALGLYKPKYETASQDKKQFVAQY FT LLDNKPADGFLTKTEVREQVESALEPQGNQLRNLPPLASGEKRINLADYDHWPQQHGDA FT EVALWADDKTGAFSLTIDDNFENEHDTWRSLAQQYGFKFSWFVITSLIKDPNKWRTLAA FT EGHEIGSHTVTHEDKGSTLDPAHLHSEYADSQALLNTIEGVRATTLAYPFGSGREDIAA FT EYYIAARGTVGLPNPADSINYMNTQSLSVRPGSLELTNQAANGNSVEAMVKTLVDPNHK FT VWSASYYRGWSNMLVHSLNESGKTPSDGVTRTSRDLTQYLLTLLDTYRDQIWVGRYGDI FT VRYSQQRDTAHIVVTRKDDRKITFNLTDRMDDTLFDYPLTVKVRVDDAWSDIGATQAGE FT PIPFVETIRDGKRYLLVKAVPDKGSVSIVPDAASPLNVVNGAVTSEQMLSAIAAPGLGL FT DLGEFNALGAGKKRMVGSRLLEVRPADGYADAAALQDALDAAVEEANNAPSLSENASLS FT DLKVNGVTIAGFAPETYAYDIMLPEGTTALPVVSFKVADTGKATAVLQNAPALPGTAKV FT TVTAEDNWTVATYTLRFQVRISALQRVNTAPDASAMRTAIENAALGLVLAAYNGLTSEQ FT KNSVAASVLTHRPATGYADVQAVQAELNAALPKINAPLLAHAIVDQLNPDTVSTANWTN FT LYGGTSGRKGGVYMKFNIASLAGLEADAIGDAKVQFFTTREGTVIGYAAPSSWEAPLTW FT NTQPLADLKNSNMAALAEIGRTAVQATGANYEMNITQYVKDAAAADKTELSLVLLGSNN FT TNITMQKIPTAFALSVTLATYGEPNPEPSPLAAVNEAGDAAAMQGAIAAVELDLNLTAY FT NGLTAAQRIDVAQALLDNRPAAGYAHALAVQVALDAAVAAAQPANQAPGGTLAASAEQL FT QPGQQLELTVGVSDASRFTGADILVHYDPQALTFATELYEGVRMLKAEAIASLQANYQV FT AAAMAEQPGTIKILLFTAGAGQPLSGTLPLFKLRASVKDDAQTGVSTAVSLSDFELTFE FT GEDSVWPDTTRAAVSLQIAAHPVEADKTALIAKIAHAQALLTGATVGANPGQYPQAAYD FT ALADAIGLAEEKRDLTGVSQAAVDEAVASLGTAEQQFLNAVIPGVPADLTALNAAIAKA FT QRLHDNGPYGEKIGQYPQSAKVPLKSALDAAKAVGGSGASSQESVNAAAASLNGAIQTF FT ERSLVTLVGGGATKVGIRDLSIVAKYYGVTSSDPNWGKVSAAAIDGGNEITIEVLAAVA FT RMILADWAAGQ" XX SQ Sequence 8333 BP; 1843 A; 2349 C; 2676 G; 1465 T; 0 other; gatctggtca acggcgagcc cgtattgcgc gacgtcgcgc tgaagtacga gacgggactc 60 ccgaacttcc tccgaagcat cggcggcggc tacgagatcg gcagacgcgg cagtctggtg 120 ggcgaaataa gcagcatgaa cgagatcgcc aaggccggct tcaatatgta tgccgagagc 180 gattggatca aaccgccgtt cccggtgctg accttcaccg cggaagagcg caaagcgatc 240 gacgatgcgc tggtcaacct gaatccttat atggacgaat acgagcaaaa agtatttatg 300 ggcgcacagg acattaacgc gacctgggat gcgtacctgg ccgagctgga taagctgaag 360 ctgcaaagcg cggtcgatgc gtacaacagc gcgtacgcgc gttacaaaac ggaatcgaaa 420 taagaagcag gcatcaagga catccgaata tatggcgaaa tacggatttc tccaatactc 480 ggatgtcctg ctttttcaag ggttctgtaa gcggttccaa ggaggattgg caaatgatcg 540 gattcgaaaa attatcatac tcaaaaggag gatttaagaa atgagatttt cctggaaaaa 600 gcttgtgagc gcggcgctcg tcatggcgct gctcgtcggt attgtctacc cggcggcatc 660 gggacgaggc gccgtggctt cggccgcgtc cggcacgact gtagaactcg ttccgacgga 720 cgatgcgttc acttccgccg ttgccaagga tgcgaatgcc aacgggacct ggatgcaatt 780 aaagggcagc attggcggac aaagatacat ttacatgaag ttcgatctga cggcgcttgc 840 cggcgtggag gccgaccgga tcgaaaacgc gaaagtatgg ctgaagaaga tggggacgaa 900 cggaaccgcg atgacggtcg gtttgagagc cgtcgacgat acgagctggt cggaatcgac 960 cttgacctgg aataatgcgc ctgtttacgg gagccaggta cttagtcagc agtccgtatt 1020 gagtacgccg gatgtttact atccattcga tctcgacgaa tatttgaaaa cgcagcttgc 1080 cgcaggcaag tccaagcttg cgatcgcttt tgtaccgata tccacgttaa atgaaaatat 1140 ggagttctac gccagagaaa gcaccgcaaa tacgccgaag ctagtcgttg aattgaagga 1200 cgaaccgcct gcgccgaccg ggctcatgca gcttgtccaa tcgttcggcg gccataacaa 1260 gggtcatctg cgcgtcgttg aattcgatgc gacgcctgct tcgactaccg gcaacggcac 1320 ggtaggcatt acggcggacg gagcggcgcc cgccgccgcg gcggacttcc cgatcgcgct 1380 tcgcttcggg acggatggca ctatcaaggc cgcgaacgca gccggcttcg agtcgaagac 1440 gccggtcaat tatacggcgg gacaaaaata ccatgtgaaa gcgatgatca atctgtcgct 1500 gggcacgtac gatctgtggc tgacgcctcc gaatgccggg cagcccgtct tgctggcggc 1560 cgactacgcg ttcgctgcga gtgcgcctgc cttgaacgat attggcggcg tccatgcaac 1620 ggccgatgcg caatcgaccg atgtcccggc cgttgcgaat gccagattaa tcgcggacca 1680 tttcgtatcc aaggctccgt tcaaggacga gcagggacaa agcctggcga ttcggctgga 1740 gtcggacaat agccttgcga atcgatccta tgccatcaaa ttcgatatga acctgacggg 1800 caatccgctg gagacggacg cgctgatcag ctatgcggac cgctcggtga cgcttaacgg 1860 attcccggat ctggcttata tcgtgcgttc caacttcggg aacttcgacg tccgcaacga 1920 caacgtgtat gcctcttccc atccttcgac cgcccaatcg aaccgaacct atcaggtgga 1980 ggtgcgaatc aacccggcga gcggtacggg ccagccgacc cggacttacg atgtatggat 2040 tgcgcccgaa ggagagcagc cggttcagct ggccgatcaa tttaaagcgc gcaactatgc 2100 gaatacaggc tatgcgctga acaatatcgg gcaggcgttc gtttacagcc aggccgacgg 2160 gctgctgtcg atcgacaacc atgtcgtgca ggacggacag cgtctggatg aagcgctcgc 2220 ccgcgtcaat gccgcaagcg gcgaggcggc gatgacggcg gcgttggaat ccaacgcgct 2280 ggggcttccg atggaacgtt atcggctcat ggatgcggcc aaacgtgcgc aggtggcgca 2340 ggacgtgttg gccggcaggc cggccgaagg ctacgcgcat gcgttgagcg tgcaggccgt 2400 attcgtatcc gccgtggcca accgtctgga tacggagaac ccgacggccc ccgcgaacgt 2460 gcaggtcgcg atctccaata cgatgcaggc gcacgtcagc tggacggcat cttcggacga 2520 tacgggcata ctgtactaca aagttttccg tgacggcgcc ttggtcggta cggttacgaa 2580 cgcgacgagc ttcgtcgaca acggacttgc gccggcaacc gaatatacgt atgtcgtaaa 2640 agcgtacgac ctggtgctga aagaagccgt atcgcagcct gcgaccgcga ccagtccggg 2700 cgagcaggcg caggttcgca ttccgttcag cgccgaagcg atcgcgacgg ctttcggaca 2760 gcctttattg gactacaatc tggaaacgca ttcaggcacg ttgaaatggg tcatggagtg 2820 gcgggaggaa tacgaaaaga gcgccaacgc gcttaagctg ttgacgctgc tgtccgcgtc 2880 cgctccggac tatatcggtc cggacggcgt tacgaccgct tcggccaagg cgctgcagca 2940 tctccggtcc gtgacggcgg gaggaaacga gcccggcttc gccggcaacg gcctgtcggg 3000 gcaaggctat atgccgctgc tgagcgcgat cgtgatggcc aaaaagaagg cccccgcgat 3060 ttggaacgcg ctgaccgccg ccgagaagga aaagctcgat ctgatgattc tggcgggtct 3120 ctatggcgcc aagttcgctt acgacgacga aaacgacaat aaaacgggca tcgacgccac 3180 cgggaacttc gacaaggaat ggaacccgaa ccatcggtcc ggcatcgcgg gcgccatcat 3240 ggcgatgtac tatttcgaag acgcccagtg gctgaacgac cagatgcgca gctttaacta 3300 tgacgactgg ctggcgagac tgaccgcggc cggattgacg aatgttcgca cgatctacca 3360 gaactcgggc aagacgctga ccgaacgcga aatccgcaag gatgcggcag gcgacggctt 3420 cgtttacaaa gggcatccgc taagccagcc gggcaaaatc atggccgaat tcgtgaatta 3480 tacgttttcg catcccgtct ctccggtcgg cggctttgac agcggcatcg ggaaataccg 3540 gggttacatc gtcgacgggc aggacgatct gccgaatctc ggcgcggaca gcatgggctt 3600 cgaattcgat acgctggacg cgaacggcaa gcgaagcagc ctagtttacg tattcatggg 3660 ctggaagccg aacgtggatg cgatcacgcc cgtattgctg ctcgataata tcgactcagg 3720 gttgacgagc gccgaaacgc gggatgtcgt aagcaggctt agcatcggaa cgacggatat 3780 gctgtacaag aacgagcatg gctatatgac ttacgccaag ggcgtgaatg aaggcgtgaa 3840 gtcgctgaac ggaccgattc tgacgatcaa cgaagagata tggaaccgga tcctgaataa 3900 ccctgcggcg ccgatggaag cggtcaatca agcttcgagc gctggacaga tgcgtacggc 3960 gctggaagcg agcgcgctcg ggatgattct gtacggctac ggcgccttgt ccgagacggg 4020 caaaaacgct gtcgcgcagc atgtgctgga cgcgcgcccg gccgccggct atgcgaataa 4080 agccgccgcg caaaatgaat tgtacgaagg cgtacgtctg caagccctgc tcgccttatc 4140 gcaggcgcag acggccgagc agatgcgttc ggcgctggaa tcgcgggcgc tcggccttta 4200 caagccgaag tatgagacgg ccagccagga caagaaacag ttcgtggccc aatatttgct 4260 ggacaacaag ccggcggacg gcttcctgac gaagaccgag gtgcgcgagc aggtcgaatc 4320 cgcgctggag ccgcagggca atcagcttcg gaatctgccc ccgctcgcat ccggcgagaa 4380 acggatcaac ctggcggatt acgatcattg gccgcagcag cacggcgacg cggaggtggc 4440 gttgtgggcc gacgacaaga cgggcgcctt ctcgctgacg atcgacgaca attttgaaaa 4500 cgaacacgat acttggcgca gcctggccca gcaatacggc tttaaattca gctggtttgt 4560 cattacgagc ttgatcaaag acccgaacaa atggcgtacg cttgccgcgg aagggcatga 4620 gatcggctcg cacaccgtta cgcacgagga caaaggcagc acgctggacc cggcgcacct 4680 gcacagcgaa tatgccgact cgcaagcgct gctgaacacg atcgagggcg ttcgcgcaac 4740 gacgctggcg tatcctttcg gcagcggcag agaagatatc gccgccgaat attatatcgc 4800 cgcccgggga acggtcgggc tgccgaatcc ggcggacagc attaattaca tgaacacgca 4860 gtcgctaagc gttcgtccgg gcagtcttga gctgaccaat caagcggcga acggcaacag 4920 cgtagaggcg atggtgaaga cgcttgtcga tcccaaccac aaagtgtgga gcgcctccta 4980 ttatcgcggc tggagcaata tgctcgtcca tagtctgaat gaaagcggca agacgccttc 5040 ggacggcgtg acgcggacga gccgcgatct gacgcaatac ctgctgacgc tgctcgatac 5100 gtatcgcgat cagatctggg tgggccgtta cggagacatc gtgcgctaca gtcaacaacg 5160 ggatacggcg cacatcgtcg tgacgcggaa ggacgatcgg aaaatcacct ttaatctgac 5220 cgaccgcatg gacgatacgt tgttcgatta cccgctcacg gtgaaggtac gcgtcgacga 5280 tgcctggagc gatatcggcg cgacgcaagc cggcgagccg attccgttcg tcgagacgat 5340 ccgggacggc aagcggtatt tgctcgtgaa ggccgttccc gacaagggca gcgtcagcat 5400 cgtgccggat gccgcttcgc cgctcaatgt cgtcaatgga gccgttacgt ccgaacaaat 5460 gctgtcggcc atcgccgctc ccggcctggg actcgatctt ggcgaattca acgcccttgg 5520 cgctgggaaa aagcggatgg ttggcagccg cctgctggag gtccgtccgg ccgacggtta 5580 tgctgatgcg gcggcgctgc aggatgcgct tgacgcggca gtggaggaag cgaacaatgc 5640 gccttcgtta agcgagaacg ccagcttaag cgatctgaag gtcaacggcg ttacgatcgc 5700 cgggtttgct ccggaaacgt atgcgtacga tatcatgctg ccggaaggaa cgacggcgct 5760 gccggtcgtc agcttcaagg tggcggatac cggcaaggcg acggccgtgc ttcagaatgc 5820 gccggcgctt cccggaacgg cgaaggtgac cgtgacggcg gaggacaatt ggacggtggc 5880 gacgtatacg ctccgcttcc aggttcgcat atcggcactg cagcgggtca atacggcccc 5940 ggacgcgtcg gccatgcgaa ccgccatcga gaatgccgcg ctcgggcttg tgctcgctgc 6000 gtacaatggc ttgacgagcg agcagaaaaa cagcgtagcg gcttcggtgc tgacccatcg 6060 tcccgcgacg gggtatgccg acgttcaggc ggtgcaggcg gagctgaacg ccgcactccc 6120 gaagatcaat gcgccgctcc tggcgcatgc catcgtggac cagttgaatc cggataccgt 6180 cagtaccgcc aactggacga atctgtacgg cggaacctcg ggccgcaagg gcggcgttta 6240 tatgaaattt aatattgcgt cgttggcggg cctggaggcc gacgcgatcg gagacgcgaa 6300 ggttcagttt tttacaacca gggaaggaac ggtgatcggt tacgccgccc cgagcagctg 6360 ggaagcgccg ctgacctgga atacgcagcc gctcgccgat ctgaaaaaca gcaatatggc 6420 ggcgctggcc gagatcggca gaactgcggt tcaggcgacg ggagccaatt acgaaatgaa 6480 catcacgcag tacgtgaagg atgccgcggc ggcagacaag acggagctgt ccctggtcct 6540 tctggggtcg aacaatacca atatcaccat gcagaagatt ccgacggcat tcgccttgtc 6600 cgtcacgctg gcgacatacg gcgaaccgaa tcccgagcct tctccgctcg cggccgtcaa 6660 cgaggccggc gatgcggcgg cgatgcaggg cgcgatcgcg gcagtcgagc ttgatctgaa 6720 cctgaccgcg tacaacggct tgacggcggc gcagcggatc gacgttgcgc aggcgctgct 6780 ggacaatcgg ccggcggcag gctacgcgca tgcgctcgcg gtccaggtcg cgctcgatgc 6840 cgccgtggcg gcggcgcagc cggcgaatca agctccgggc ggcacgctcg cggccagcgc 6900 agaacagctg cagccggggc agcaactgga gctgacggtc ggcgtaagcg acgcaagccg 6960 ctttaccggc gcggatatcc tcgtccatta cgatccgcag gcgctgacgt tcgctaccga 7020 actgtacgaa ggcgtccgga tgctgaaggc agaagcgatc gcgtcgctgc aggcgaatta 7080 ccaggtggcg gccgcgatgg ccgaacaacc gggaacgatc aagattctgc tgttcacggc 7140 cggcgcgggg cagccgctct caggaacctt gccgctgttc aagctgcgcg catcggtcaa 7200 ggacgacgcg cagaccggcg tatctaccgc cgtgtcgctg tccgacttcg agctgacctt 7260 cgaaggggaa gattcggtct ggccggatac gactagagcg gccgtctccc tgcagattgc 7320 ggcgcatccg gttgaagcgg acaaaacggc gttgatcgcc aagatcgcgc atgcgcaggc 7380 gcttctgacc ggtgcgacgg tcggcgcgaa tccgggacaa tatccgcagg cggcctatga 7440 cgcgctggcg gatgcgatcg ggctggcgga ggagaagcgc gatctgacgg gcgtgtccca 7500 agcggcggtc gacgaagccg tcgcctcgct ggggaccgcc gaacagcaat tcctgaacgc 7560 ggtcattccg ggcgtaccgg ccgacctgac ggcgttgaac gccgcgatcg cgaaggcgca 7620 gaggcttcac gacaacggtc cgtacggaga aaagatcggc cagtatccgc aatcggccaa 7680 ggttccgctc aagtcggcgc tcgatgcggc caaggcggtc ggcgggagcg gcgcttccag 7740 ccaggagagc gtcaatgcgg ccgccgcttc cctgaacggg gccatccaga cgttcgagcg 7800 ctcgctggtc acgctcgtcg ggggcggagc gaccaaagtc ggcattcgcg acctgtccat 7860 cgttgccaag tactacggcg taacgagttc ggacccgaac tggggcaagg tgtcggcggc 7920 cgcgatcgac ggaggaaatg aaattacgat cgaggtgctg gctgccgtcg ccagaatgat 7980 tctggccgat tgggcggcag gacaataaag gaagaacggg caggcatgca gatgaacagg 8040 ttaccggatg cggctcggcg agccgcgtcc ggtcttcgtg ccgcgaaggg tggaggcgta 8100 gggatgaggc tgaggatgag gatcaaattt acgatcgcca tgctggtaag ctgcttatgg 8160 atgaccggga tgcctgtcgc attcggcgcg gcggccaacg aatccgcggc gttcgagatg 8220 acgagcggcg gcgacggttc gctggcggtc gtcatctccg cccacgggac aagcgacgtc 8280 tatgcgtatg aattaaagct gacgttcgac gagcggcggc tcaagctgga gaa 8333 // |
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