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EBI DbfetchID AB006823; SV 1; linear; genomic DNA; STD; PRO; 2812 BP. XX AC AB006823; XX DT 04-SEP-1997 (Rel. 52, Created) DT 14-APR-2005 (Rel. 83, Last updated, Version 3) XX DE Bacillus sp. DNA for alpha-amylase, complete cds. XX KW alpha-amylase. XX OS Bacillus sp. OC Bacteria; Firmicutes; Bacillales; Bacillaceae; Bacillus. XX RN [1] RP 1-2812 RA Arai M.; RT ; RL Submitted (26-AUG-1997) to the EMBL/GenBank/DDBJ databases. RL Motoo Arai, Osaka Prefecture University, Department of Applied Biological RL Chemistry; Gakuen-cho 1-1, Sakai, Osaka 599, Japan RL (E-mail:monger@main.biochem.osakafu-u.ac.jp, Tel:+81-722-52-1161, RL Fax:+81-722-52-0341) XX RN [2] RA Sumitani J., Nagae H., Kawaguchi T., Arai M.; RT "Bacillus animal type alpha-amylse. Cloning, nucleotide sequence of the RT gene, and comparison with other amylases"; RL Unpublished. XX RN [3] RX DOI; 10.1042/0264-6021:3500477 RX PUBMED; 10947962. RA Sumitani J., Tottori T., Kawaguchi T., Arai M.; RT "New type of starch-binding domain: the direct repeat motif in the RT C-terminal region of Bacillus sp. no. 195 alpha-amylase contributes to RT starch binding and raw starch degrading"; RL Biochem. J. 350:477-484(2000). XX FH Key Location/Qualifiers FH FT source 1..2812 FT /organism="Bacillus sp." FT /strain="No.195" FT /mol_type="genomic DNA" FT /db_xref="taxon:1409" FT RBS 167..181 FT CDS 182..2284 FT /codon_start=1 FT /transl_table=11 FT /gene="baa" FT /product="alpha-amylase" FT /db_xref="GOA:O24781" FT /db_xref="HSSP:1B0I" FT /db_xref="InterPro:IPR020003" FT /db_xref="UniProtKB/TrEMBL:O24781" FT /protein_id="BAA22082.1" FT /translation="MPALYQGVIADVRAKRKRLQVLAKMVLIALLGTLLSATAFAAPAS FT AAAPGPKDATAVMFSWTWNAIARECTENLGPAGYGYVQTSPPQEHIQGAAWWTHYQPVS FT YKIESRFGTRAEFKAMVDTCHAAGVKVIADAVINHMTGQSAGGTGWAGSTFQHYDYPGI FT YQSQDFHSCRRNIANYQDRWEVQECNLVNLADLNTSSSYVQGKIAAYLNDLVSLGVDGL FT RIDAVKHIAASDMQGILSKVNDRARLYIVQEVIRANEPIQPEEYTSNGDIHEFAFARKL FT KEAFNGGTINWLTTGNGIGPTWAGFLPNANAAVFVDNHDTERNGETLTYKDGANYDLAQ FT IFTLAWNYGSPSIHSGYSFSNNDAGPALAGNGEVIDPVCGQNGWTCKHAQTGIENMVGF FT RTQTYGTAVVNKWDNGSSAIAFGRGDKGYVAINRGSALTRTFQTSLPAGNYCNVIVGLP FT NSTGCSAGGVVTVDAAGTFTATVDQNSAFALHVGAKAGTQQPGPGAGDMKVYYSTSKGW FT SDYKIHYRVGTGAWTTAPGAGMTAACAGWVSYTVPAGSTGATAAFNNGSGTWDNNNTSN FT YALSGAVSTVNGGVVGHTDPCTESAPAPADTAVVFYSTNKGWSAYNIHYRVGTGAWTTA FT PGSAMTAACTGWMTASIPLGGASGITAAFNNGAGTWDNNAGADYSVGSGYRQVKDGVVS FT TGNPCA" FT sig_peptide 182..319 XX SQ Sequence 2812 BP; 536 A; 1013 C; 846 G; 417 T; 0 other; ggatcccaac cgactgtaag cgtttccaat tttttgcgca aaacgcttgc gccggaatat 60 gggagcaccc tatcttgatg ggagtcgtca tcgtgacgcg accgccgcag tgaagcggcg 120 gtgtcagcaa attcgggatt cccggcccgg atctgccgat tcacctagga gccatacctc 180 catgccagcc ctctaccagg gcgtcattgc cgacgtccga gcaaagagaa aacgcttgca 240 agttttggcc aagatggtcc tcatcgccct ccttggcacg ctgctttcgg ccaccgcttt 300 cgccgccccg gcgagcgccg cagcccccgg ccccaaggac gccaccgccg tcatgttctc 360 ctggacatgg aacgcgatcg cccgtgaatg caccgagaac ctcggccccg ccggctacgg 420 ctacgtgcag acctcgcctc cccaggaaca catccaaggc gccgcgtggt ggacccatta 480 ccagcccgtc agctacaaga tcgagtcccg cttcggcacc cgggcggagt tcaaggccat 540 ggtggacacc tgccacgccg caggcgtgaa ggtgatcgcg gacgccgtca tcaaccacat 600 gaccggccag agcgccggcg gcaccggctg ggccggttcc accttccagc actacgacta 660 cccgggcatc taccagtccc aggacttcca ctcctgccgc cgcaacatcg ccaactacca 720 ggaccgctgg gaggtacagg agtgcaacct cgtgaacctc gcggacctga acacttcctc 780 gtcctacgtc caaggaaaga ttgcggcata cctgaacgat ctcgtctcgc tcggcgtcga 840 cggcctccgc attgacgccg tcaagcacat cgcggcgagc gacatgcagg gcatcctgtc 900 caaggtgaac gaccgcgccc gcctctacat cgtccaggaa gtcatccgcg ccaacgagcc 960 catccagccc gaggaataca ccagcaacgg tgacatccac gagttcgcct tcgcccgtaa 1020 gctcaaggaa gccttcaacg gcggcaccat caactggctg accaccggca acggaatcgg 1080 ccccacctgg gccggcttcc tgccgaacgc caacgccgca gtgttcgtgg acaaccacga 1140 caccgagcgc aacggtgaaa ccctcaccta caaggacgga gccaactacg acctcgccca 1200 gatcttcacc ctcgcctgga actacggctc gccgtccatc cactcgggct attccttctc 1260 gaacaacgac gccggcccgg cactcgccgg aaacggcgaa gtgattgatc cggtatgcgg 1320 ccagaacggc tggacctgca agcacgccca gacgggcatc gagaacatgg tgggcttccg 1380 cacccagacg tacggcaccg ccgtcgtgaa caaatgggac aacggctcca gcgccatcgc 1440 gttcggccgg ggagacaagg gctacgtggc gataaaccgc ggcagcgccc tcacccgcac 1500 cttccagacc tccctgcccg cgggcaacta ctgcaacgtg atcgtcggcc tgcccaactc 1560 caccggctgc tcggccggcg gcgtggtgac ggttgacgcc gcgggcacct tcacggccac 1620 cgtggaccag aactccgcgt tcgcactgca cgtcggcgcg aaggccggaa cgcagcagcc 1680 cggaccgggc gcgggcgaca tgaaggtgta ctactcgacg tcgaagggct ggagcgacta 1740 caagatccac taccgcgtgg gtaccggcgc ctggaccacc gctcccggtg ccggcatgac 1800 ggccgcctgc gccggctggg tctcgtacac cgtcccggcc ggctccaccg gagccaccgc 1860 cgccttcaac aacggcagcg gcacctggga caacaacaac accagcaact acgcactcag 1920 cggcgcggtc agcacagtga acggcggcgt cgtggggcat acggacccct gcaccgaaag 1980 cgcgcccgcc ccggccgaca cagccgtggt gttctactcc accaacaagg gctggtccgc 2040 ctacaacatc cactaccgcg tgggtacggg cgcctggacc accgcgccgg gcagcgccat 2100 gacggccgcg tgcaccggct ggatgaccgc ctccatcccc ctgggcggag cctccggaat 2160 caccgctgcc ttcaacaatg gcgcgggcac ctgggataac aacgccggcg ccgattacag 2220 cgttggcagc ggttaccggc aggtgaagga cggcgtggtc agcacgggaa acccctgcgc 2280 ctgacccgcg aagacctcac gcacccggga cctccggggg cgtgagccgg gaaggaggcg 2340 gcgtcggatc atccggcgcc gccttctcct gcttaacgcg gcgttagagc tgatgatccc 2400 ataccgtcag ctggagacgc accgtgtcgc cgttccagtg agggtcgacg acggcggagt 2460 cgccctcgtg cgggctgctc accaaagccc tgctcaccac ggcgcggctc acctcgcgct 2520 cccggcggct gcctcggcca gctccggttc cgcggcagca aggccgcgtt ccggcgtcgc 2580 gagtccgagg ttctcccgca gggtgctgcc ggcgtattgg ctggggtaga cgccgcgttc 2640 ctgcagttct gggaccaggt ggttcacgat gtcgtccagg ccgctgggga tgatccacgg 2700 cgaaatgttg aagccatcca cggcgcctac gcgggcgtat tcggccaagg tgtccgccac 2760 ggaactgtag gagcccgtga aggtggaatc gatacgggcg gtccgggagc tc 2812 // |
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