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EBI DbfetchID AB004845; SV 1; linear; genomic DNA; STD; PRO; 6240 BP. XX AC AB004845; XX DT 21-AUG-1998 (Rel. 56, Created) DT 12-NOV-2007 (Rel. 93, Last updated, Version 3) XX DE Clostridium josui cipA, celD genes for scaffolding protein, exoglucanase, DE complete cds. XX KW . XX OS Clostridium josui OC Bacteria; Firmicutes; Clostridia; Clostridiales; Clostridiaceae; OC Clostridium. XX RN [1] RP 1-6240 RA Sakka K.; RT ; RL Submitted (16-JUN-1997) to the EMBL/GenBank/DDBJ databases. RL Kazuo Sakka, Mie University, Laboratory of Applied Microbiology; 1515 RL Kamihamacho, Tsu, Mie 514-8507, Japan (E-mail:sakka@bio.mie-u.ac.jp, RL Tel:81-592-31-9621, Fax:81-592-31-9634) XX RN [2] RX PUBMED; 9696784. RA Kakiuchi M., Isui A., Suzuki K., Fujino T., Fujino E., Kimura T., RA Karita S., Sakka K., Ohmiya K.; RT "Cloning and DNA sequencing of the genes encoding Clostridium josui RT scaffolding protein CipA and cellulase CelD and identification of their RT gene products as major components of the cellulosome"; RL J. Bacteriol. 180(16):4303-4308(1998). XX FH Key Location/Qualifiers FH FT source 1..6240 FT /organism="Clostridium josui" FT /mol_type="genomic DNA" FT /db_xref="taxon:1499" FT -35_signal 25..30 FT -10_signal 48..53 FT RBS 212..218 FT CDS 225..3713 FT /codon_start=1 FT /transl_table=11 FT /gene="cipA" FT /product="scaffolding protein" FT /db_xref="GOA:O82830" FT /db_xref="HSSP:1G43" FT /db_xref="InterPro:IPR018452" FT /db_xref="UniProtKB/TrEMBL:O82830" FT /protein_id="BAA32429.1" FT /translation="MRKKSLAFLLALTMLVTLLGAQLTAFAADTGVISVQFNNGSSPTS FT SSSIYARFKVTNTSGSPINLADLKLRYYFTQDENKQMTFWCDHAGYLSGNNYMDVTSKV FT SGTFNEVSPAVTNADHYLEVALSSDAGSLPAGGSIEIQTRFARNDWSNFDQSNDWSYTS FT AGSYMDWQKIAAFVGGTLVYGSTPNGDDNPTQDPKISPTSISAKQGQFSDAVIALTPNG FT NTFNGITELQSNQYVKGTNQVTILASYLNTLPANSTKTLTFDFGVGSKNPKLTINVGES FT GNTNGLKVSVGTAVGAPGDTVTVPVTFADVAKVNNVGTCNFYLGYDASLLDVVSVDAGP FT IVKNAAVNFSSSASNGTISFLFLDNTITDELITSDGVFANITFKIKSTATQGTTPITFK FT DGGAFGDGTMSKIASVIKTSGSVVISPDPTNALKVTVGTAEGNVGETVTVPVTFADVAK FT VNNVGTCNFYLAYDASLLDVVSVDAGPIVKNAAVNFSSSASNGTISFLFLDNTITDELI FT TSDGVFANITFKLKNVSTKTTTPITFKDGGAFGDGTMSKITTVIKTNGSVTIIPGDPEP FT TEDLNVAVGTAEGNVGETVTVPVTFANVAKVNNVGTCNFYLAYDASLLDVVSVDAGPIV FT KNAAVNFSSSASNGTISFLFLDNTITDELITSDGVFANITFKLKNVSTKTTTPISFKDG FT GAFGDGNMAKIATVVKTNGSVTIIPGDPEPTEDLNVAVGTAEGNVGDTVTVPVTFANVA FT KVNNVGTCNFYLTYDASLLDVVSVDAGPIVKNAAVNFSSSASNETISFLFLDNTITDEL FT ITSDGVFANITFKLKSVSTKTTTPISFKDGGAFGDGTMAKIATVVKTNGSVTIIPGDPE FT PTEDLNVAVGTAEGNVGDTVTVPVTFANVAKVNNIGTCNFYLTYDASLLDVVSVAAGPI FT VKNAAVNFSSSASNGTISFLFLDNTITDELITSDGVFANITFKLKSVSTKTTTPISFKD FT GGAFGDGTMAKIATVVKTNGSVTIDVGEPVVTGLGVKIASVTGKTGDTITVPVTLSNVA FT TVGNVGTCNFYITYDPTLLQAVSATAGDIVINAPVNFSSSINATNGTISILFLDNTITD FT QPIASDGVITNLTFKVLGSSSTTTPIAFKAGGAFGNGNMAKISDITFTNGSAKLN" FT RBS 3813..3822 FT CDS 3828..5987 FT /codon_start=1 FT /transl_table=11 FT /gene="celD" FT /product="exoglucanase" FT /db_xref="GOA:O82831" FT /db_xref="HSSP:1FAE" FT /db_xref="InterPro:IPR002105" FT /db_xref="UniProtKB/TrEMBL:O82831" FT /protein_id="BAA32430.1" FT /translation="MSKNFKRVGAVAVAAAMSLSIMTTTSINAAASPVNKVYQERFESM FT YNKIKDPSNGYFSEEGIPYHSVETLMVEAPDYGHVTTSEAMSYYMWLEAMYGRFTGDFS FT GFNKSWTTTEKYLIPTEKDQPNSSMSRYDANKPATYAPEFQDPSKYPSPLDTSQPVGRD FT PINSQLTSAYGTSMLYGMHWLLDVDNWYGFGVRADGTGKPSYINTFQRGEQESTWETIP FT QPCWDEHKFGGQYGFLDLFTKDTGTPAKQFKYTNAPDADARAVQATYWANEWAKEQGKS FT VSTEVGKASKMGDYLRYSFFDKYFRKIGQPTQAGTGYDSAHYLLSWYYAWGGGVDSTWS FT WIIGCSHNHFGYQNPFAAWVLSTDSSFKPKSSNGATDWAKSLDRQLEFYQWLQSSEGAI FT AGGATNSWNGRYESIPSGTSTFYGMGYVENPVYADPGSNTWFGMQVWSMQRVAELYYKT FT GDTRAKNLLDKWAKWVNSEIKFNADGTFQIPGTLDWEGQPDTWDPTQGYTGNPNLHVKV FT VNYNTDLGCASSLANTLTYYAAKSGDTTSKENAKKLLDAMWNNYSDSKGISTIEQRGDY FT HRFLDQEVYVPAGWTGKMPNGDVIKSGVKFIDIRSKYKQDPEWQTMVAALQAGQVPTQR FT LHRFWAQSEFAVANGVYAILFPEGSNLGDVNGDETVDAIDLAMLKKYLLNSSTSIVAGN FT ADMNGDGAIDAIDYALLKKALLANQ" XX SQ Sequence 6240 BP; 2137 A; 1080 C; 1283 G; 1740 T; 0 other; agattaagga ggtgaaaata tttattcagt tatatcggtg tgcctgtaaa aatgaaacat 60 tttaaaacat ttttagagta aggcagttta aaagcatctt ttatttaaaa gtacttttta 120 ttaataactg ccttaaactg ataaaagact gaatattggt gataaaaccc atagtcaaaa 180 ggccagatgg ttttcatgta aaataaaaaa taggaggttt acaaatgcgt aaaaagtctt 240 tagcattttt gctagcacta acaatgttgg tgacattatt aggagctcag cttacagctt 300 ttgcagctga tactggcgtc atatcagttc aatttaataa tggtagttca ccaacatcat 360 caagttcaat atacgctaga tttaaagtta caaacacaag tggttcacca atcaacttgg 420 cagatttgaa acttagatat tattttactc aggatgaaaa taagcaaatg acattctggt 480 gtgaccatgc aggttatctg agtggtaaca actatatgga tgttacttca aaggtatctg 540 gaacatttaa tgaagtaagc cctgcagtta caaatgcaga tcattatctt gaagttgcat 600 taagcagtga tgcaggaagt cttccagctg gaggatctat agaaattcaa accagatttg 660 caagaaacga ctggtctaat tttgatcaat caaatgactg gtcatatact tcagctggtt 720 catacatgga ttggcagaaa attgctgctt tcgtaggcgg aactcttgta tatggttcaa 780 cacctaatgg tgatgacaac ccaacacaag acccaaaaat cagtcctact tctatttctg 840 caaaacaggg acaattctca gacgctgtaa tagctcttac accaaatgga aatacattta 900 atggaattac tgagttgcag agtaaccaat atgtaaaggg aacaaaccaa gtaacaatat 960 tggctagcta tttgaataca ttgccagcaa atagcacaaa aactcttaca tttgatttcg 1020 gtgtaggttc aaagaatcct aaattgacta tcaatgtagg tgaaagtggt aatacaaatg 1080 gtcttaaggt ttcagtagga acagctgttg gtgctcctgg tgatacagta acagttcctg 1140 ttacatttgc tgatgtagca aaagtaaaca acgtaggaac atgtaacttc tatcttggat 1200 atgatgcaag tcttttggat gtagtatcag tagatgcagg tccaatagtt aagaatgcag 1260 cagtaaactt ctcaagcagt gcaagcaacg gaacaatcag cttcctgttc ttggacaaca 1320 caatcactga tgaattgatt acttcagatg gtgtgttcgc aaatatcaca tttaagatta 1380 agagtactgc tacacaaggt acaacaccaa taaccttcaa agatggagga gcttttggtg 1440 acggtactat gtcaaagata gcttcagtta ttaagacaag tggtagtgta gttataagtc 1500 cagatcctac aaatgctctt aaagtaacag taggaacagc agaaggtaat gttggagaaa 1560 cagtaacagt tcctgttaca tttgctgatg tagcaaaagt aaacaacgta ggaacatgta 1620 acttctatct tgcatatgat gcaagtcttt tggatgtagt atcagtagat gcaggtccaa 1680 tagttaagaa tgcagcagta aacttctcaa gcagtgcaag caacggaaca atcagcttcc 1740 tgttcttgga taacacaatc actgacgaat tgattacttc agatggtgtg tttgcaaata 1800 tcacattcaa attaaagaat gtatcaacta aaacaacaac accaataacc ttcaaagacg 1860 gaggagcatt tggtgacggt actatgtcaa agataactac agttatcaag acaaacggta 1920 gtgtaacaat tattcctggt gacccagaac ctacagaaga tcttaacgta gcagtaggaa 1980 cagcagaagg taatgttgga gaaacagtaa cagttcctgt aacatttgct aatgtagcaa 2040 aagtaaacaa cgtaggaaca tgtaacttct atcttgcata tgatgcaagt cttttggatg 2100 tagtatcagt agatgcaggt ccaatagtta agaatgcagc agtaaacttc tcaagcagtg 2160 caagcaatgg aacaatcagc ttcctgttct tagataacac aatcactgat gaattgatta 2220 cttcagatgg tgtgtttgca aatatcacat tcaaattaaa gaatgtatca actaaaacaa 2280 caacaccaat aagcttcaaa gatggaggag catttggtga cggtaatatg gcaaagatag 2340 ctacagttgt taaaacaaac ggtagtgtaa caattattcc tggtgatcca gaacctacag 2400 aagatcttaa cgtagcagta ggaacagcag aaggtaatgt tggagataca gtaacagttc 2460 ctgtaacatt tgctaacgta gcaaaagtaa acaacgtagg aacatgtaac ttctatctta 2520 catatgatgc aagtcttttg gatgtagtat cagtagatgc aggtccaata gttaagaatg 2580 cagcagtaaa cttctcaagc agtgcaagca acgaaacaat cagcttcttg ttcttagata 2640 acacaatcac tgacgaattg attacttcag atggtgtgtt tgcaaatatc acattcaaat 2700 taaagagtgt atcaactaaa acaacaacac caataagctt caaagacgga ggagcatttg 2760 gtgacggtac tatggcaaag atagctacag ttgttaagac aaatggtagt gtaacaatta 2820 ttcctggtga tccagaacct acagaagatc ttaatgtagc agtaggaaca gcagaaggta 2880 atgttggaga tacagtaaca gttcctgtaa catttgctaa cgtagcaaaa gtaaacaaca 2940 taggaacatg taacttctat cttacatatg atgcaagctt gttagatgta gtatcagtag 3000 ctgcaggtcc aatagttaag aatgcagcag taaacttctc aagcagtgca agcaacggaa 3060 caatcagctt cttgttctta gataacacaa tcactgacga attgattact tcagatggtg 3120 tgtttgcaaa tatcacattc aaattaaaga gtgtatcaac taaaacaaca acaccaataa 3180 gcttcaaaga cggaggagca tttggtgacg gtactatggc aaagatagct acagttgtta 3240 agacaaatgg tagtgttact atcgatgttg gtgagcctgt tgttacagga cttggagtaa 3300 agattgcttc agtaacagga aaaactggtg atactataac agtacctgta actctcagca 3360 atgttgctac agtaggtaat gtaggaacat gtaatttcta tattacatat gacccaacct 3420 tgttgcaggc tgtatcagca acagctggtg atatagtaat aaatgcacct gttaacttct 3480 caagcagtat caatgcaaca aatggtacta tcagtattct tttccttgat aacactataa 3540 ctgatcaacc tatcgcaagt gatggagtaa tcactaacct tactttcaaa gtattaggtt 3600 cttcaagcac aactactcct atcgctttca aagcaggtgg agcattcggt aatggtaaca 3660 tggcaaaaat cagtgatatc acattcacaa atggaagtgc aaaacttaat taattattga 3720 atttaaattt tccatacttt atggctgaaa ggccataaag tatggagctt ttaaaaactt 3780 atagcagttt tactgccata aaattcaaat aataaggaag gtgtaaaatg agtaagaatt 3840 ttaagagagt aggagcagtt gcagttgctg cagcaatgtc attatcaata atgacaacaa 3900 ctagcataaa tgcagcagca agcccggtaa acaaggtgta ccaggagcgt ttcgaatcca 3960 tgtacaacaa gattaaggat ccttcaaacg gatacttcag tgaagaggga attccttatc 4020 actcagtaga aacactcatg gttgaagctc cagactacgg acatgttaca acaagtgaag 4080 caatgtccta ttatatgtgg ttggaagcaa tgtatggaag attcacagga gatttttcag 4140 gttttaacaa gtcttggact actactgaaa aatatttgat ccctacagaa aaggatcagc 4200 ctaattcaag tatgagcaga tatgatgcta acaagcctgc tacatacgct ccagaattcc 4260 aggatccaag caagtatcct tcaccattgg atactagcca acctgttggt agagatccaa 4320 ttaattcaca attgacttca gcatatggaa ccagcatgct gtacggaatg cattggttac 4380 ttgatgttga caactggtat ggatttggag taagagctga tggaacagga aagccatcat 4440 atatcaatac tttccaaaga ggtgaacaag agtcaacttg ggaaaccatt cctcaaccat 4500 gttgggatga acataaattt ggtggacagt atggattctt ggatctcttt acaaaggata 4560 caggtactcc agcaaaacaa ttcaaataca caaatgcacc ggacgctgat gctcgtgcag 4620 ttcaagcaac ttactgggct aatgaatggg caaaagaaca aggtaagagc gtaagtacag 4680 aagtaggtaa ggcatcaaag atgggtgatt acctcagata ttcattcttt gataagtatt 4740 tcagaaagat tggacaacct actcaagctg gtacaggata tgattcagca cattatttgc 4800 tttcatggta ctatgcatgg ggtggtggag ttgactcaac ttggtcatgg ataatcggtt 4860 gcagtcataa ccattttgga taccagaatc catttgcagc ttgggtactt tcaacagatt 4920 caagcttcaa gcctaagtca tcaaatggtg caactgactg ggcaaagagc ttggacagac 4980 agcttgaatt ctatcagtgg ttgcagtcat cagaaggtgc tattgccggt ggtgcaacaa 5040 actcatggaa cggacgttat gaatcaattc cttcaggtac ttcaacattc tacggaatgg 5100 gttatgtaga aaaccctgta tatgctgacc caggtagtaa cacttggttt ggtatgcagg 5160 tatggtcaat gcagcgtgta gctgaattgt actacaagac tggtgatact agagctaaga 5220 acctcttaga caaatgggca aaatgggtta acagtgaaat taagttcaat gctgacggaa 5280 cattccagat tcctggtaca cttgattggg aaggacagcc agatacttgg gacccaacac 5340 aaggatacac tggaaatcca aacttgcatg ttaaagttgt aaactacaat actgaccttg 5400 gttgtgcttc ttcacttgca aacacattga cttactatgc tgctaaatca ggagatacaa 5460 cttctaaaga gaatgctaag aagcttttag atgctatgtg gaataattac agcgattcaa 5520 agggtatttc aactattgaa caacgtggtg attaccacag attccttgac caggaagttt 5580 atgtaccagc tggttggact ggaaaaatgc ctaacggtga tgtaattaaa tctggtgtta 5640 agttcataga tattcgttct aagtacaagc aggatcctga atggcagaca atggtagctg 5700 ctttacaggc tggacaggtt cctacacaga gattacaccg tttctgggct cagagtgaat 5760 ttgcagttgc aaatggtgtt tatgcaatac tcttcccaga aggatctaat ttgggagatg 5820 ttaacggcga cgaaactgta gacgctattg accttgctat gcttaagaaa tatctgttaa 5880 acagcagtac ttcaatagtt gctggaaatg cagatatgaa cggtgatgga gcaattgacg 5940 ctattgacta tgctctttta aagaaggcac ttcttgctaa tcaatagtat taattaatgc 6000 tataacgcat atgtaacctt aaagtccgga cggtatttga tgtgattaga ttattcattc 6060 ttgtgccgtc cgggcttatg agttacaaag aaaaataaga aaaggattaa ggtaagaaca 6120 tgattaaagg ttcaagctta aaaagaatta aatcacttgt tatgatggct atattcagtg 6180 tttcaataat cacaacggcc atcgtgtcaa gtgcagctga tcaaattcct ttcccatata 6240 // |
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