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EBI DbfetchID AB004098; SV 1; linear; genomic DNA; STD; PRO; 2854 BP. XX AC AB004098; XX DT 20-FEB-1998 (Rel. 54, Created) DT 14-APR-2005 (Rel. 83, Last updated, Version 4) XX DE Bacillus sp. KSM-522 gene for endo-1,4-beta-glucanase, complete cds. XX KW endo-1,4-beta-glucanase. XX OS Bacillus sp. OC Bacteria; Firmicutes; Bacillales; Bacillaceae; Bacillus. XX RN [1] RP 1-2854 RA Hitomi J.; RT ; RL Submitted (21-MAY-1997) to the EMBL/GenBank/DDBJ databases. RL Jun Hitomi, Kao Corporation, Biological Science Laboratories; Akabane 2606, RL Ichikaimachi, Tochigi 321-34, Japan (E-mail:382728@kastanet.kao.co.jp, RL Tel:0285-68-7400, Fax:0285-68-7403) XX RN [2] RX PUBMED; 9438981. RA Hitomi J., Hatada Y., Kawaminami S., Kawai S., Ito S.; RT "Amino acid sequence and stereoselective hydrolytic reaction of an RT endo-1,4-beta-glucanase from a Bacillus strain"; RL Biosci. Biotechnol. Biochem. 61(12):2004-2009(1997). XX FH Key Location/Qualifiers FH FT source 1..2854 FT /organism="Bacillus sp." FT /strain="KSM-522" FT /mol_type="genomic DNA" FT /clone="pBEG38" FT /note="neutrophilic Bacillus" FT /db_xref="taxon:1409" FT CDS 467..2377 FT /transl_table=11 FT /product="endo-1,4-beta-glucanase" FT /EC_number="3.2.1.4" FT /note="belonging to the cellulase family E2" FT /db_xref="GOA:P28622" FT /db_xref="InterPro:IPR001701" FT /db_xref="InterPro:IPR001956" FT /db_xref="InterPro:IPR008928" FT /db_xref="InterPro:IPR008965" FT /db_xref="InterPro:IPR012341" FT /db_xref="InterPro:IPR018221" FT /db_xref="UniProtKB/Swiss-Prot:P28622" FT /protein_id="BAA24918.1" FT /translation="MTRRWSFLVQCFTFKKKEGVRSRYMSDYNYVEVLQKSILFYEAQR FT SGKLPESNRLNWRGDSGLEDGKDVGHDLTGGWYDAGDHVKFGLPMAYSAAVLAWTVYEY FT REAYEEAELLDDMLDQIKWATDYFLKAHTGPNEFWAQVGDGNADHGWWGPAEVMPMNRP FT AFKIDEHCPGTEVAAQTAAALAAGSIIFKETDAPYAAKLLTHAKQLYAFADQYRGEYTD FT CVTNAQPFYNSWSGYIDELIWGGIWLYLATNDQTYLNKALKAVEEWPKDWDYTFTMSWD FT NTFFLSQILLARITKEKRFIESTERNLDYWSTGFVQNGKVERITYTPGGLAWLDQWGSL FT RYTANAAFLAFVYADWVSDQEKKNRYQTFAIRQTHYMLGDNPQNRSYVVGFGKNPPMHP FT HHRTAHGSWSNQLTTPSSHRHTLYGPLVGGPNRQDQYTDDISDYVSNEVATDYNAAFTG FT NGAAVWSGQSKLPNFPPKEKVEDEFFVEAAVMSNDTTSTQIKAILYNRSGWPARSSQSL FT SFRYYVNLSEIFAKGFTDKDIQVTAVYNEGASLSPLTVYDASSHIYFTEIDFTGVAIFP FT GGESLHKKEIQFRLSAPNGANIWDASNDYSFQGLTSNMQKTARIPVFDQGDLVFGTLPN FT K" XX SQ Sequence 2854 BP; 863 A; 537 C; 659 G; 795 T; 0 other; atttcaaaag aagatttaca aaaacgagga aaagtgtcta agaccatcct tgagtatttg 60 gatcgtcaag gctgtttaga agcacttcct gatcaaaacc agctttcttt gttctaatgt 120 gaaaaatgaa gatgcgccct taaagggcgt tcttttttgt atatacaata atattattat 180 gttaactaaa tattttaact ggaattattt tcttgtttgt tcatatatct tgatttaacg 240 tcgtgttttg agagaagaga ttgtatgtca ttctttctac ccaaatagag caaaaaagag 300 aatttagccg tctgaaatgt tctcatacga catatgatca ttttgggtac tgtcacatga 360 agaatcagga gaaaaccctc ggtgagcgtc aacgtgatgg ctgtttacta tgcacatttt 420 tgaaacacgc ttgattcttt tcaacactgt acaaaacacg aacggaatga caaggaggtg 480 gtcatttctt gttcaatgtt tcacgtttaa gaaaaaagaa ggggtgagaa gtaggtacat 540 gtcagattac aactatgtag aggtgcttca aaaatccatt ttgttttacg aagcccagcg 600 ttccggaaag cttcctgaaa gcaatcgtct taactggcga ggggattctg gactagagga 660 tgggaaagat gttggtcatg atttaacagg cggctggtac gatgctggtg atcatgtgaa 720 gttcggactt ccgatggctt actcggcagc cgtgcttgca tggacagtct atgagtaccg 780 agaagcttac gaagaggcag agctgcttga tgatatgtta gatcaaatca agtgggcaac 840 cgattatttt ttgaaagccc atacaggtcc aaatgaattt tgggcacaag taggcgatgg 900 gaacgcagat catggctggt ggggtccagc agaagtgatg ccgatgaacc ggccggcatt 960 taaaattgat gaacattgtc caggaacaga agtagccgcc caaaccgcag ccgctttagc 1020 agcaggttca attattttta aagaaactga tgcgccttat gcagcaaagc ttctcaccca 1080 tgcaaaacag ctttatgcat ttgctgacca atatcgcggt gagtatacag attgtgtcac 1140 caatgcgcag ccattttata actcttggag cggctatatt gatgagctca tttggggtgg 1200 aatatggctg tacttggcga caaatgacca gacctattta aacaaagcac taaaagcagt 1260 agaagaatgg ccgaaggatt gggattatac gtttacgatg tcgtgggaca ataccttttt 1320 cttgtcgcaa attttacttg cgagaatcac aaaggagaaa aggtttatcg aatcgacgga 1380 gcgtaatctc gattactggt cgacgggttt tgttcaaaat ggaaaagtag aaagaatcac 1440 ttatacgcct ggcggactag cgtggttaga tcaatggggt tcacttcgtt atacagcaaa 1500 tgctgcattt ttagcgtttg tgtacgcaga ttgggtctct gatcaagaaa aaaagaatcg 1560 ttaccaaacg tttgcgatca ggcaaacaca ctatatgtta ggggataatc cgcaaaatag 1620 aagctatgtc gttgggtttg gcaaaaatcc gccgatgcat ccacaccatc gaactgcaca 1680 tggctcatgg tctaatcagc tgacaactcc ttcttctcat cggcacacgc tttatggacc 1740 gcttgttggg ggtcctaatc gacaggatca gtataccgat gacatctctg actatgtatc 1800 aaacgaggta gcaacagact ataatgccgc ctttactgga aatggtgcag ctgtttggtc 1860 agggcagtca aagctgccga attttccgcc taaagaaaaa gtggaagatg agttttttgt 1920 agaggcagct gtcatgagta acgatacaac atctactcag atcaaagcca ttttatacaa 1980 tcgatcaggc tggccggcca gaagtagtca atcactttct tttagatatt atgtcaatct 2040 gagcgagata tttgcgaagg gattcactga taaagatatt caagtgacag ctgtctacaa 2100 tgaaggcgct tccttatctc cgttaacggt gtatgacgca tcatcccata tctattttac 2160 agaaatcgat tttactggcg tagctatttt tccaggaggc gaatcgcttc ataagaagga 2220 aatacagttc cggttatctg cgccaaatgg tgcgaatata tgggatgcct caaatgatta 2280 ttcctttcaa ggattaacat ctaatatgca aaaaacagcg agaattcctg tttttgatca 2340 aggtgattta gtatttggta cgcttccgaa taaataaata taaagggtga ggacatgaca 2400 aataaagaac ggtttttaac actttatcat caaatgaaaa acgagacgaa tggatatttt 2460 tcaaaagaag gcattccgta tcattccgtt gagacactca tttgtgaggc acctgattac 2520 gggcatatga caacttcaga agcttacagc tattggttat ggcttgagca ttgtatggtc 2580 actacacagg cgattggggc aactagaagc gggttgggat tatatggaga agtttatcat 2640 tccagtaaac gaagatggac atgacgacag gcgcatatga gttcttacaa tccatccagc 2700 caaggcacat acgcttcaga aaaaccatat ccagatcaat atccgagcca gtgagggtgc 2760 tcgcctgctg gtcaagatcc aatgacaaga actgaggcac ttacggacga ttaaacgttt 2820 taacatgact ggctctggtg tggcactggt tgat 2854 // |
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