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EBIDatabasesDatabase BrowsingDbfetch

EBI Dbfetch

ID   AB003087; SV 1; linear; genomic DNA; STD; PRO; 492 BP.
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AC   AB003087;
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DT   06-MAY-1997 (Rel. 51, Created)
DT   14-APR-2005 (Rel. 83, Last updated, Version 3)
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DE   Bacillus stearothermophilus DNA for Pyrophosphatase, partial cds.
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KW   Pyrophosphatase.
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OS   Geobacillus stearothermophilus
OC   Bacteria; Firmicutes; Bacillales; Bacillaceae; Geobacillus.
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RN   [1]
RP   1-492
RA   Samejima T.;
RT   ;
RL   Submitted (11-APR-1997) to the EMBL/GenBank/DDBJ databases.
RL   Tatsuya Samejima, Aoyama Gakuin University, Department of Chemistry,
RL   College of Science and Engineering; Chitosedai 6-16-1, Setagaya-ku, Tokyo
RL   157, Japan (E-mail:samejima@fatima.chem.aoyama.ac.jp, Tel:03-5384-1111,
RL   Fax:03-5384-6200)
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RN   [2]
RA   Satoh T., Ishii K., Koyama M., Sakurai N., Kaji H., Hachimori A., Irie M.,
RA   Samejima T.;
RT   "Primary structure,overexpression,characterization and crystallization o f
RT   inorganic pyrophosphatase from Bacillus stearothermophilus.";
RL   Unpublished.
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DR   StrainInfo; 46428; ATCC 12016.
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FH   Key             Location/Qualifiers
FH
FT   source          1..492
FT                   /organism="Geobacillus stearothermophilus"
FT                   /strain="ATCC 12016"
FT                   /mol_type="genomic DNA"
FT                   /db_xref="taxon:1422"
FT   CDS             <1..>492
FT                   /codon_start=1
FT                   /transl_table=11
FT                   /gene="pMK2PPA"
FT                   /product="Pyrophosphatase"
FT                   /note="Bst.PPase"
FT                   /note="Region amplified by PCR is base 21-472
FT                   (corresponding to amino acid Val 7 to Ala 158"
FT                   /note="The expression vector contains start codon ATG and
FT                   stop codon TGA and TAA"
FT                   /db_xref="GOA:O05724"
FT                   /db_xref="InterPro:IPR008162"
FT                   /db_xref="UniProtKB/Swiss-Prot:O05724"
FT                   /protein_id="BAA19837.1"
FT                   /translation="AFENKIVEAFIEIPTGSQNKYEFDKERGIFKLDRVLYSPMFYPAE
FT                   YGYLQNTLALDGDPLDILVITTNPTFPGCVIDTRVIGYLNMVDSGEEDAKLIGVPVEDP
FT                   RFDEVRSIEDLPQHKLKEIAHFFERYKDLQGKRTEIGTWEGPEAAAKLIDECIARYNEQ
FT                   K"
XX
SQ   Sequence 492 BP; 143 A; 118 C; 125 G; 106 T; 0 other;
     gcctttgaga ataagattgt cgaagcgttt atcgaaattc caactgggag ccaaaacaaa        60
     tacgaattcg acaaagaacg gggcattttc aagctcgacc gcgtcttata ttcaccgatg       120
     ttttatccgg ctgaatacgg ctatttgcaa aatacgttgg cgctggatgg cgacccgctc       180
     gacattttgg tcatcacgac aaacccgacg ttcccgggct gtgtcattga tacgcgtgtc       240
     atcggctatt tgaacatggt cgacagcggc gaagaagatg cgaaattgat cggcgttccg       300
     gtcgaagatc cgcgctttga cgaagttaga tcgattgaag atctcccgca gcacaaactg       360
     aaagaaatcg cccacttctt tgaacgctac aaagacttgc aagggaaacg gacggaaatc       420
     ggcacatggg aggggccgga agcagccgcc aaattgatcg acgaatgcat cgcccgctat       480
     aacgaacaaa aa                                                           492
//
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