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EBIDatabasesDatabase BrowsingDbfetch

EBI Dbfetch

ID   CAL94762; SV 1; linear; genomic DNA; STD; PRO; 840 BP.
XX
PA   AM406670.1
XX
DE   Azoarcus sp. BH72 2-dehydro-3-deoxyphosphooctonate aldolase
XX
OS   Azoarcus sp. BH72
OC   Bacteria; Proteobacteria; Betaproteobacteria; Rhodocyclales;
OC   Rhodocyclaceae; Azoarcus.
OX   NCBI_TaxID=62928;
XX
FH   Key             Location/Qualifiers
FH
FT   source          1..840
FT                   /organism="Azoarcus sp. BH72"
FT                   /strain="BH72"
FT                   /mol_type="genomic DNA"
FT   CDS             complement(AM406670.1:2346959..2347798)
FT                   /transl_table=11
FT                   /gene="kdsA"
FT                   /locus_tag="azo2145"
FT                   /product="2-dehydro-3-deoxyphosphooctonate aldolase"
FT                   /function="3-deoxy-D-manno-octulosonic acid (KDO)
FT                   8-phosphate synthass (LPS) O-antigen"
FT                   /EC_number="2.5.1.55"
FT                   /note="2-dehydro-3-deoxyphosphooctonate aldolase (EC
FT                   2.5.1.55) (Phospho-2- dehydro-3-deoxyoctonate aldolase)
FT                   (3-deoxy-D-manno-octulosonic acid 8- phosphate synthetase)
FT                   (KDO-8-phosphate synthetase) (KDO 8-P synthase) (KDOPS).
FT                   Homology to kdsA of Pseudomonas aeruginosa of 45%
FT                   (gi|6647573|sp|Q9ZFK4|KDSA_PSEAE(SwissProt (ExPASy))).
FT                   Tigrfam: 3-deoxy-8-phosphooctulonate synthase. This HMM
FT                   describes 3-deoxy-8-phosphooctulonate synthase. Alternate
FT                   names include 2-dehydro-3-deoxyphosphooctonate aldolase,
FT                   3-deoxy-d-manno-octulosonic acid 8-phosphate and KDO-8
FT                   phosphate synthetase. It catalyzes the aldol condensation
FT                   of phosphoenolpyruvate with D-arabinose
FT                   5-phosphate:,phosphoenolpyruvate + D-arabinose 5-phosphate
FT                   + H2O = 2-dehydro-3-deoxy-D-octonate 8-phosphate +
FT                   phosphate, In Gram-negative bacteria, this is the first
FT                   step in the biosynthesis of 3-deoxy-D-manno-octulosonate,
FT                   part of the oligosaccharide core of lipopolysaccharide.
FT                   Pfam: DAHP synthetase I family. No signal peptide. No
FT                   TMHs.,"
FT                   /note="High confidence in function and specificity"
FT                   /db_xref="GOA:A1K7F7"
FT                   /db_xref="InterPro:IPR006269"
FT                   /db_xref="UniProtKB/Swiss-Prot:A1K7F7"
FT                   /func_characterised="identical sequence"
FT                   /protein_id="CAL94762.1"
FT                   /translation="MNLCGFEVGLDKPVFLIAGPCVIESREMAFETAGALKDICAEVGI
FT                   PFIYKSSYDKANRSSGKSYRGMGMEKGLDILADVKKQLGVPVLTDVHSIEEIPAVAAVV
FT                   DVLQTPAFLCRQTDFIHAVASCGRPVNIKKGQFLAPGDMKNVVDKAREANGGADNIMVC
FT                   ERGASFGYNNLVSDMRSLAIMRETGCPVVFDATHSVQLPGGQGTASGGQREFVPVLARA
FT                   AVAVGVAGLFMETHPNPAQALSDGPNAWPLPRMKALLSTLKAIDELVKSRGLLETEA"
XX
SQ   Sequence 840 BP; 148 A; 265 C; 275 G; 152 T; 0 other; 732382088 CRC32;
     atgaatctgt gtggattcga ggtcggcctc gacaagccgg ttttcctgat tgccggcccg        60
     tgcgtgatcg agtcgcgcga gatggcgttc gaaaccgccg gcgcgctcaa ggacatctgc       120
     gctgaagtcg gcattccctt catctacaag tcgtcctacg acaaggccaa ccgcagctcg       180
     ggcaagtcct atcgcggcat gggcatggag aagggactgg acatcctcgc ggacgtcaag       240
     aagcagctcg gcgtgccggt gctgaccgac gtgcattcga tcgaggagat cccggccgtc       300
     gcggcggtgg tcgatgtgct gcagaccccc gcctttctgt gtcgccaaac cgacttcatc       360
     cacgcggtgg cgtcctgtgg ccgcccggtg aatatcaaga aaggccagtt tctcgcgccc       420
     ggcgacatga agaacgtggt cgacaaggcc cgggaagcca acggcggcgc tgacaacatc       480
     atggtgtgcg agcgcggcgc gtccttcggc tacaacaacc tggtgtccga catgcgctcc       540
     ctcgcgatca tgcgcgagac cggctgcccg gtggtgttcg atgccaccca ctcggtgcaa       600
     cttccgggcg ggcagggcac ggcatcgggt ggtcagcgcg aattcgtacc cgtcctcgcc       660
     agggcggctg tcgcggtcgg cgtggcgggt cttttcatgg aaactcaccc gaatcccgcg       720
     caggcgcttt ccgatggccc caatgcctgg cccttgccgc gcatgaaggc gttgctcagc       780
     acgctgaagg cgatcgacga actggtcaag tcgcgcgggc tgctggaaac cgaggcctga       840
//
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