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EBIDatabasesDatabase BrowsingDbfetch

EBI Dbfetch

ID   CAL94592; SV 1; linear; genomic DNA; STD; PRO; 954 BP.
XX
PA   AM406670.1
XX
DE   Azoarcus sp. BH72 2,3-dihydroxyphenylpropionate 1,2-dioxygenase
XX
OS   Azoarcus sp. BH72
OC   Bacteria; Proteobacteria; Betaproteobacteria; Rhodocyclales;
OC   Rhodocyclaceae; Azoarcus.
OX   NCBI_TaxID=62928;
XX
FH   Key             Location/Qualifiers
FH
FT   source          1..954
FT                   /organism="Azoarcus sp. BH72"
FT                   /strain="BH72"
FT                   /mol_type="genomic DNA"
FT   CDS             complement(AM406670.1:2171448..2172401)
FT                   /transl_table=11
FT                   /gene="mhpB"
FT                   /locus_tag="azo1975"
FT                   /product="2,3-dihydroxyphenylpropionate 1,2-dioxygenase"
FT                   /EC_number="1.13.11.-"
FT                   /note="2,3-dihydroxyphenylpropionate 1,2-dioxygenase
FT                   similar in function (51% identity) to TrEMBL:
FT                   P54711,PF02900, IPR004183 The LigAB enzyme (a
FT                   protocatechuate 4,5-dioxygenase EC: 1.13.11.8), of
FT                   Sphingomonas paucimobilis oxidizes protocatechuate (or
FT                   3,4-dihydroxybenzoic acid,PCA). The enzyme belongs to the
FT                   class III extradiol-type catecholic dioxygenase family,
FT                   which catalyzes the ring-opening reaction of
FT                   protocatechuate and related compounds . Other members of
FT                   this family include 3,4-dihydroxyphenylacetate
FT                   2,3-dioxygenase (EC: 1.13.11.15) and
FT                   2,3-dihydroxyphenylpropionate 1,2-dioxygenase. FUNCTION:
FT                   Extradiol cleavage of 2,3-dihydroxyphenylpropionic acid.
FT                   COFACTOR: Ferrous ion. PATHWAY: 3-hydroxyphenylpropionate
FT                   degradation. SUBUNIT: Homotetramer. No Signal Peptide or
FT                   TMH being reported as present."
FT                   /note="High confidence in function and specificity"
FT                   /db_xref="GOA:A1K6Y7"
FT                   /db_xref="InterPro:IPR004183"
FT                   /db_xref="UniProtKB/Swiss-Prot:A1K6Y7"
FT                   /func_characterised="identical sequence"
FT                   /protein_id="CAL94592.1"
FT                   /translation="MSAMLQCMSHTPLKGYFDPAADVVEEVAALAAAVREEVAHFDPEV
FT                   VYLFAPDHYNGFFLDLMPQFCLGIEATSVGDYQTPKGPLRVPRALAEACAGAVIARGID
FT                   LAFSYRMQVDHGFAQTLVEVLGGLARYPVVPIMINAVAPPLASFQRARLLGAAVGEFAR
FT                   GQGGRALFIASGGMSHNPPIPQIATATDPEVIERIVAGRNPTPEARAARQQRTVAAAQA
FT                   FAAGDSPLRPLNPDWDRALMKRLCSRDWAAIDAYRNDEVSAAAGASAHELKSWVAATAA
FT                   MESATAGQWDARERYYRAIPEWLAGFGAMSGLGRHP"
XX
SQ   Sequence 954 BP; 130 A; 340 C; 342 G; 142 T; 0 other; 3851285306 CRC32;
     atgagcgcga tgctgcaatg catgtcgcat accccgctga agggctattt cgacccggcg        60
     gcggacgtgg tggaggaggt ggccgcgctg gccgccgcgg tgcgcgagga ggtggcccac       120
     ttcgatcccg aggtggtcta tctcttcgcc ccggaccact acaacggctt tttcctcgac       180
     ctgatgccgc agttctgcct cggcatcgag gccacatcgg tgggggacta ccagactccg       240
     aaagggccgt tgcgggtgcc gcgcgcgctg gccgaggcct gcgccggcgc ggtgatcgcg       300
     cgcggcatcg acctcgcatt ctcctaccgc atgcaggtgg accacggctt tgcgcagacg       360
     ctggtggaag tgctgggcgg gctcgcccgc tacccggtgg tgccgatcat gatcaacgcg       420
     gtggcaccgc cgctggcgag tttccagcgc gctcgcctgc tgggcgcggc ggtgggtgag       480
     tttgcccgcg ggcagggcgg gcgcgcgctc ttcatcgctt ccggcggcat gtcgcacaac       540
     ccgcccatcc cgcagatcgc caccgccacc gaccccgagg tgatcgagcg catcgtcgcc       600
     ggacgcaacc ccacgccgga ggcgcgcgcg gcccgccagc agcgcacggt tgccgcggcg       660
     caggcgttcg ccgcgggcga cagtcccttg cggccgctca atccggactg ggaccgcgcg       720
     ctgatgaagc ggctgtgcag ccgcgactgg gcggcgatcg acgcctaccg caacgacgaa       780
     gtcagcgccg cggccggtgc ttccgcccac gagctgaaga gctgggtggc cgccacggcg       840
     gcgatggaat ccgcgaccgc cggccagtgg gatgcgcgcg agcgctacta ccgcgccatc       900
     cccgaatggc tggcgggctt cggcgccatg tcgggccttg gccgccaccc ctga             954
//
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