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EBIDatabasesDatabase BrowsingDbfetch

EBI Dbfetch

ID   CAL16248; SV 1; linear; genomic DNA; STD; PRO; 798 BP.
XX
PA   AM286690.1
XX
DE   Alcanivorax borkumensis SK2 tRNA (Guanine-N1)-methyltransferase
XX
OS   Alcanivorax borkumensis SK2
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Oceanospirillales;
OC   Alcanivoracaceae; Alcanivorax.
OX   NCBI_TaxID=393595;
XX
FH   Key             Location/Qualifiers
FH
FT   source          1..798
FT                   /organism="Alcanivorax borkumensis SK2"
FT                   /strain="SK2"
FT                   /mol_type="genomic DNA"
FT   CDS             AM286690.1:893297..894094
FT                   /transl_table=11
FT                   /gene="trmD"
FT                   /locus_tag="ABO_0800"
FT                   /product="tRNA (Guanine-N1)-methyltransferase"
FT                   /function="is one of several nucleases operating together
FT                   with the tRNA-modifying enzymes before the formation of the
FT                   mature tRNA. It catalyses the
FT                   reaction:S-adenosyl-L-methionine + tRNA ->
FT                   S-adenosyl-L-homocysteine + tRNA
FT                   containingN1-methylguanine, methylating guanosine(G) to
FT                   N1-methylguanine (1-methylguanosine (m1G)) at position 37
FT                   of tRNAs that read CUN (leucine), CCN(proline), and CGG
FT                   (arginine) codons. The presence of m1G improves the
FT                   cellular growth rate and the polypeptide steptime and also
FT                   prevents the tRNA from shifting the reading frame"
FT                   /EC_number="2.1.1.31"
FT                   /note="identified by sequence similarity; putative
FT                   InterPro: tRNA (guanine-N1-)-methyltransferase"
FT                   /note="High confidence in function and specificity"
FT                   /db_xref="GOA:Q0VRF0"
FT                   /db_xref="InterPro:IPR002649"
FT                   /db_xref="UniProtKB/Swiss-Prot:Q0VRF0"
FT                   /func_characterised="identical sequence"
FT                   /protein_id="CAL16248.1"
FT                   /translation="MMTTKPTFAVTLVTLFPEMAQAITGFGVTRRAVDNGQLQVETVNP
FT                   RDFTEDRHRTVDDRPFGGGPGMVMKVEPLAKALQAARVANPAAKVIYLSPQGQPLTQAK
FT                   ATALAEQPGLILLAGRYEGVDERLLDAEVDEQISIGDYVLSGGELPALVLIDAVSRLIP
FT                   GVLGHQDSAEQDSFSGEFENLLDCPHYTRPEVYGEQAVPPVLLSGNHELIRRWRLKQAL
FT                   GRTWQQRPDLLEARRARGLSKEEQQLLDEYIAEQPSHTAKTTD"
XX
SQ   Sequence 798 BP; 170 A; 221 C; 252 G; 155 T; 0 other; 1312817685 CRC32;
     atgatgacga cgaagcctac gtttgccgtc actctggtaa cgttgtttcc ggagatggca        60
     caagcgatta ctggctttgg cgttactcgg cgggctgtcg ataatggtca gctgcaggtg       120
     gaaacggtga accctcggga cttcaccgaa gaccgccacc gtaccgtgga tgatcgccct       180
     tttgggggcg gccctggcat ggtgatgaaa gtggagccgc tggcgaaagc cttacaggct       240
     gcacgcgtcg ccaacccggc agccaaagtg atttacctgt cccctcaggg gcagccgtta       300
     acccaggcca aggccacggc gttagcggaa cagcctggcc taatattgct ggccgggcgc       360
     tatgaaggcg tagacgagcg tttgctcgat gccgaggtgg atgagcaaat ttccatcggc       420
     gactacgtac tcagcggtgg tgaattgcca gcgctggtgc tgatcgatgc ggttagccga       480
     ttgatacccg gagtgttggg tcaccaagac tccgccgagc aggactcttt ttcgggtgaa       540
     ttcgaaaact tgctcgattg cccgcactac acccgcccgg aggtgtatgg cgagcaggca       600
     gtgccaccgg tactgctcag cggcaatcat gaactgattc gccgctggcg ccttaagcag       660
     gcgttgggac ggacctggca acagcgtcca gacctgctgg aggcccgccg ggcgcggggc       720
     ttgagcaaag aagagcaaca gctgctggac gaatacatcg ccgagcagcc gtcgcataca       780
     gcaaaaacta ctgattag                                                     798
//
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