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EBIDatabasesDatabase BrowsingDbfetch

EBI Dbfetch

ID   CAD94970; SV 1; linear; genomic DNA; STD; PRO; 480 BP.
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PA   BX248343.1
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DE   Mycobacterium bovis AF2122/97 PROBABLE DIHYDROFOLATE REDUCTASE DFRA
DE   (TETRAHYDROFOLATE DEHYDROGENASE)
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OS   Mycobacterium bovis AF2122/97
OC   Bacteria; Actinobacteria; Actinobacteridae; Actinomycetales;
OC   Corynebacterineae; Mycobacteriaceae; Mycobacterium;
OC   Mycobacterium tuberculosis complex.
OX   NCBI_TaxID=233413;
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FH   Key             Location/Qualifiers
FH
FT   source          1..480
FT                   /organism="Mycobacterium bovis AF2122/97"
FT                   /strain="AF2122/97"
FT                   /mol_type="genomic DNA"
FT   CDS             complement(BX248343.1:212204..212683)
FT                   /transl_table=11
FT                   /gene="dfrA"
FT                   /locus_tag="Mb2785c"
FT                   /product="PROBABLE DIHYDROFOLATE REDUCTASE DFRA
FT                   (TETRAHYDROFOLATE DEHYDROGENASE)"
FT                   /EC_number="1.5.1.3"
FT                   /note="Mb2785c, dfrA, len: 159 aa. Equivalent to Rv2763c,
FT                   len: 159 aa, from Mycobacterium tuberculosis strain H37Rv,
FT                   (100.0% identity in 159 aa overlap). Probable dfrA
FT                   (alternate gene name: folA), dihydrofolate reductase (EC
FT                   1.5.1.3), equivalent to O30463|FOLA DIHYDROFOLATE REDUCTASE
FT                   from Mycobacterium avium (see citation below) (181 aa),
FT                   FASTA scores: opt: 802, E(): 4.5e-48, (70.2% identity in
FT                   161 aa overlap); and Q9CBW1|FOLA|ML1518 DIHYDROFOLATE
FT                   REDUCTASE from Mycobacterium leprae (165 aa), FASTA scores:
FT                   opt: 782, E(): 1e-46, (70.55% identity in 163 aa overlap).
FT                   Also highly similar to many e.g.
FT                   Q9K168|DYR_NEIMB|FOLA|NMB0308 from Neisseria meningitidis
FT                   (serogroup B) (162 aa), FASTA scores: opt: 469, E():
FT                   3.8e-25, (46.65% identity in 163 aa overlap);
FT                   P12833|DYR3_SALTY|DHFRIII from Salmonella typhimurium (162
FT                   aa), FASTA scores: opt: 367, E(): 4e-18, (45.4% identity in
FT                   141 aa overlap); Q59408|DYRC_ECOLI|DHFRXIII from
FT                   Escherichia coli strain RA33.2 (165 aa), FASTA scores: opt:
FT                   313, E(): 2.2e-14, (41.9% identity in 136 aa overlap); etc.
FT                   Contains PS00075 Dihydrofolate reductase signature. BELONGS
FT                   TO THE DIHYDROFOLATE REDUCTASE FAMILY."
FT                   /db_xref="GOA:P0A547"
FT                   /db_xref="InterPro:IPR017925"
FT                   /db_xref="UniProtKB/Swiss-Prot:P0A547"
FT                   /func_characterised="identical sequence"
FT                   /protein_id="CAD94970.1"
FT                   /translation="MVGLIWAQATSGVIGRGGDIPWRLPEDQAHFREITMGHTIVMGRR
FT                   TWDSLPAKVRPLPGRRNVVLSRQADFMASGAEVVGSLEEALTSPETWVIGGGQVYALAL
FT                   PYATRCEVTEVDIGLPREAGDALAPVLDETWRGETGEWRFSRSGLRYRLYSYHRS"
XX
SQ   Sequence 480 BP; 78 A; 141 C; 181 G; 80 T; 0 other; 3172031864 CRC32;
     atggtggggc tgatctgggc tcaagcgaca tcgggtgtca tcggccgcgg cggcgacatc        60
     ccctggcgct tgcccgagga ccaggcgcat ttccgggaga tcaccatggg gcacacgatc       120
     gtgatgggcc ggcgcacatg ggattcgctg ccggctaaag tccggccgct gcccggccgg       180
     cgaaatgtcg tactgagccg ccaagctgac tttatggcca gcggggctga ggttgtcggt       240
     tcactcgagg aggcgctgac cagcccggag acgtgggtga tcggaggcgg acaagtctat       300
     gcgctggcgc tgccgtacgc gaccagatgt gaggttaccg aggtcgacat cggcctgccg       360
     cgcgaagccg gtgacgcgct ggcccccgtg ctggacgaga catggcgggg cgagacgggg       420
     gagtggcgct tcagccggtc cgggttgcgg taccggttgt acagctacca ccgctcatga       480
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