spacer
spacer
EBIDatabasesDatabase BrowsingDbfetch

EBI Dbfetch

ID   CAD94917; SV 1; linear; genomic DNA; STD; PRO; 1407 BP.
XX
PA   BX248343.1
XX
DE   Mycobacterium bovis AF2122/97 PROBABLE SOLUBLE PYRIDINE NUCLEOTIDE
DE   TRANSHYDROGENASE STHA (STH) (NAD(P)(+) TRANSHYDROGENASE [B-SPECIFIC])
DE   (NICOTINAMIDE NUCLEOTIDE TRANSHYDROGENASE)
XX
OS   Mycobacterium bovis AF2122/97
OC   Bacteria; Actinobacteria; Actinobacteridae; Actinomycetales;
OC   Corynebacterineae; Mycobacteriaceae; Mycobacterium;
OC   Mycobacterium tuberculosis complex.
OX   NCBI_TaxID=233413;
XX
FH   Key             Location/Qualifiers
FH
FT   source          1..1407
FT                   /organism="Mycobacterium bovis AF2122/97"
FT                   /strain="AF2122/97"
FT                   /mol_type="genomic DNA"
FT   CDS             BX248343.1:164577..165983
FT                   /transl_table=11
FT                   /gene="sthA"
FT                   /locus_tag="Mb2732"
FT                   /product="PROBABLE SOLUBLE PYRIDINE NUCLEOTIDE
FT                   TRANSHYDROGENASE STHA (STH) (NAD(P)(+) TRANSHYDROGENASE
FT                   [B-SPECIFIC]) (NICOTINAMIDE NUCLEOTIDE TRANSHYDROGENASE)"
FT                   /EC_number="1.6.1.1"
FT                   /note="Mb2732, sthA, len: 468 aa. Equivalent to Rv2713,
FT                   len: 468 aa, from Mycobacterium tuberculosis strain H37Rv,
FT                   (100.0% identity in 468 aa overlap). Probable sthA, soluble
FT                   pyridine nucleotide transhydrogenase (EC 1.6.1.1), highly
FT                   similar to others e.g. Q983E2|MLR8366 from Rhizobium loti
FT                   (Mesorhizobium loti) (481 aa), FASTA scores: opt: 1447,
FT                   E(): 4.1e-78, (49.55% identity in 460 aa overlap);
FT                   P27306|STHA_ECOLI|STH|UDHA|B3962 from Escherichia coli
FT                   strain K12 (465 aa), FASTA scores: opt: 1267, E(): 1.7e-67,
FT                   (43.05% identity in 462 aa overlap); O05139|STHA_PSEFL|STH
FT                   from Pseudomonas fluorescens (463 aa), FASTA scores: opt:
FT                   1257, E(): 6.6e-67, (43.8% identity in 461 aa overlap);
FT                   etc. Also highly similar to CAC46308|SMC00300 PUTATIVE
FT                   OXIDOREDUCTASE PROTEIN from Rhizobium meliloti
FT                   (Sinorhizobium meliloti) (467 aa), FASTA scores: opt: 1466,
FT                   E(): 3e-79, (49.55% identity in 462 aa overlap). Shows some
FT                   similarity to MTCY359.04, E(): 3.1e-08; MTCY210.05, E():
FT                   3.4e-08. Contains ATP/GTP-binding site motif A (P-loop;
FT                   PS00017). BELONGS TO THE PYRIDINE NUCLEOTIDE-DISULFIDE
FT                   OXIDOREDUCTASES CLASS-I. COFACTOR: FAD (BY SIMILARITY)."
FT                   /db_xref="GOA:P66007"
FT                   /db_xref="HSSP:1ONF"
FT                   /db_xref="InterPro:IPR004099"
FT                   /db_xref="UniProtKB/Swiss-Prot:P66007"
FT                   /func_characterised="identical sequence"
FT                   /protein_id="CAD94917.1"
FT                   /translation="MREYDIVVIGSGPGGQKAAIASAKLGKSVAIVERGRMLGGVCVNT
FT                   GTIPSKTLREAVLYLTGMNQRELYGASYRVKDRITPADLLARTQHVIGKEVDVVRNQLM
FT                   RNRVDLIVGHGRFIDPHTILVEDQARREKTTVTGDYIIIATGTRPARPSGVEFDEERVL
FT                   DSDGILDLKSLPSSMVVVGAGVIGIEYASMFAALGTKVTVVEKRDNMLDFCDPEVVEAL
FT                   KFHLRDLAVTFRFGEEVTAVDVGSAGTVTTLASGKQIPAETVMYSAGRQGQTDHLDLHN
FT                   AGLEVQGRGRIFVDDRFQTKVDHIYAVGDVIGFPALAATSMEQGRLAAYHAFGEPTDGI
FT                   TELQPIGIYSIPEVSYVGATEVELTKSSIPYEVGVARYRELARGQIAGDSYGMLKLLVS
FT                   TEDLKLLGVHIFGTSATEMVHIGQAVMGCGGSVEYLVDAVFNYPTFSEAYKNAALDVMN
FT                   KMRALNQFRR"
XX
SQ   Sequence 1407 BP; 283 A; 456 C; 438 G; 230 T; 0 other; 2508354357 CRC32;
     atgcgtgaat acgacatcgt ggtgatcggg tcaggcccgg gcggacagaa agccgccatc        60
     gcctcggcga agctgggcaa gtccgtggcc atcgtcgaac gcggccgaat gctcggcggc       120
     gtctgcgtca acacaggcac gatcccatcc aaaacgttgc gtgaggctgt gctctacctc       180
     accggcatga accaacgcga gctgtacggc gcaagctacc gcgtgaagga ccggatcacc       240
     ccggccgacc tgttggcgcg gacccagcac gtgatcggca aggaagtcga cgtggtgcgc       300
     aaccagctga tgcgtaaccg cgtcgatctg atcgtgggcc atggccggtt catcgacccg       360
     cacaccatcc tcgtggagga ccaggcccgc agggaaaaga ccaccgtcac cggcgactac       420
     atcatcatcg ccactggcac caggccggca cggccatccg gagtcgaatt tgacgaagaa       480
     cgggtgctcg actccgacgg gatcctcgat ctcaaatcgc tgccatcctc gatggtcgtg       540
     gtcggtgccg gcgtgatcgg catcgaatac gcctccatgt tcgctgcgtt gggcaccaaa       600
     gtcaccgtcg tggagaagcg ggacaacatg ctggacttct gcgaccccga ggtcgtcgag       660
     gcgctgaaat tccacctgcg cgacctggcg gtgacattcc ggttcggcga ggaagtgacc       720
     gcggtcgatg tcggctctgc gggcaccgtg accaccctgg ccagcggcaa acagattcca       780
     gccgagaccg taatgtactc ggcgggacgt cagggacaaa ccgaccacct cgacctgcac       840
     aacgccggac tcgaggtgca gggccgcggg cggatcttcg tagacgaccg tttccagacc       900
     aaggtagacc acatctacgc cgtcggcgac gtcattggct tccccgcctt ggccgcgacg       960
     tcgatggagc aggggcggct ggccgcctac cacgccttcg gcgaaccaac cgacggaatc      1020
     accgaacttc agccgatcgg tatttattcg attcccgagg tgtcctacgt cggcgccacc      1080
     gaggtggaac tgaccaagag ctccatccca tacgaggtgg gagtggcccg ctaccgggag      1140
     ctggcccgcg gccaaatcgc cggcgactcc tacggcatgc tcaagctgct ggtttccacc      1200
     gaggatctca agctgctcgg cgtgcatatc ttcggcacca gcgccaccga gatggtgcac      1260
     atcgggcagg ccgtgatggg atgcgggggc agcgtcgagt acctggtcga cgcggtgttc      1320
     aactacccga ccttctcgga ggcctacaag aacgccgcac tggacgtgat gaacaagatg      1380
     cgcgcactca accagttccg ccgctga                                          1407
//
spacer
spacer