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EBIDatabasesDatabase BrowsingDbfetch

EBI Dbfetch

ID   CAB09074; SV 1; linear; genomic DNA; STD; PRO; 1584 BP.
XX
PA   BX842577.1
XX
DE   Mycobacterium tuberculosis H37Rv Probable L-aspartate oxidase nadB
XX
OS   Mycobacterium tuberculosis H37Rv
OC   Bacteria; Actinobacteria; Actinobacteridae; Actinomycetales;
OC   Corynebacterineae; Mycobacteriaceae; Mycobacterium;
OC   Mycobacterium tuberculosis complex.
OX   NCBI_TaxID=83332;
XX
FH   Key             Location/Qualifiers
FH
FT   source          1..1584
FT                   /organism="Mycobacterium tuberculosis H37Rv"
FT                   /strain="H37Rv"
FT                   /mol_type="genomic DNA"
FT   CDS             BX842577.1:64548..66131
FT                   /transl_table=11
FT                   /gene="nadB"
FT                   /locus_tag="Rv1595"
FT                   /product="Probable L-aspartate oxidase nadB"
FT                   /function="QUINOLINATE BIOSYNTHESIS. CATALYZES THE
FT                   OXIDATION OF L-ASPARTATE TO IMINOASPARTATE WHICH IS
FT                   CONDENSED WITH DIHYDROXYACETONE PHOSPHATE TO QUINOLINATE
FT                   UNDER THE ACTION OF QUINOLINATE SYNTHASE A [CATALYTIC
FT                   ACTIVITY : L-ASPARTATE + H(2)O + O(2) = OXALOACETATE +
FT                   NH(3) + H(2)O(2)]"
FT                   /EC_number="1.4.3.16"
FT                   /note="Rv1595, (MTCY336.09c), len: 527 aa. Probable nadB,
FT                   L-aspartate oxidase (EC 1.4.3.16). Similar to many e.g.
FT                   Q49617 L-ASPARTATE OXIDASE (QUINOLINATE SYNTHETASE) from
FT                   Mycobacterium leprae (424 aa), FASTA results: opt: 2152,
FT                   E(): 0, (82.0% identity in 400 aa overlap). Also shows some
FT                   similarity to Rv1552 frdA from Mycobacterium tuberculosis
FT                   (583 aa), FASTA results: E(): 1e-10, (35.3% identity in 566
FT                   aa overlap). HETERODIMER. THE QUINOLINATE SYNTHETASE
FT                   COMPLEX CONSISTS OF THE TWO ENZYMES QUINOLINATE SYNTHETASE
FT                   A AND B. TBparse score is 0.896."
FT                   /db_xref="GOA:P65499"
FT                   /db_xref="HSSP:1CHU"
FT                   /db_xref="InterPro:IPR005288"
FT                   /db_xref="UniProtKB/Swiss-Prot:P65499"
FT                   /experiment="experimental evidence, no additional details
FT                   recorded"
FT                   /func_characterised="identical sequence"
FT                   /protein_id="CAB09074.1"
FT                   /translation="MAGPAWRDAADVVVIGTGVAGLAAALAADRAGRSVVVLSKAAQTH
FT                   VTATHYAQGGIAVVLPDNDDSVDAHVADTLAAGAGLCDPDAVYSIVADGYRAVTDLVGA
FT                   GARLDESVPGRWALTREGGHSRRRIVHAGGDATGAEVQRALQDAAGMLDIRTGHVALRV
FT                   LHDGTAVTGLLVVRPDGCGIISAPSVILATGGLGHLYSATTNPAGSTGDGIALGLWAGV
FT                   AVSDLEFIQFHPTMLFAGRAGGRRPLITEAIRGEGAILVDRQGNSITAGVHPMGDLAPR
FT                   DVVAAAIDARLKATGDPCVYLDARGIEGFASRFPTVTASCRAAGIDPVRQPIPVVPGAH
FT                   YSCGGIVTDVYGQTELLGLYAAGEVARTGLHGANRLASNSLLEGLVVGGRAGKAAAAHA
FT                   AAAGRSRATSSATWPEPISYTALDRGDLQRAMSRDASMYRAAAGLHRLCDSLSGAQVRD
FT                   VACRRDFEDVALTLVAQSVTAAALARTESRGCHHRAEYPCTVPEQARSIVVRGADDANA
FT                   VCVQALVAVC"
XX
SQ   Sequence 1584 BP; 223 A; 531 C; 583 G; 247 T; 0 other; 369346376 CRC32;
     atggccggtc ccgcttggcg ggatgcggcc gatgttgtcg tgatcggcac gggcgttgcc        60
     gggctggcgg cggcattggc cgccgatcgc gccgggcgca gcgtcgtggt gctcagcaag       120
     gctgcccaga cgcacgtgac cgcgacacac tacgcgcaag gcggtatcgc ggtggtgctg       180
     ccggacaacg acgactcggt cgacgctcac gtcgcggaca ccttggccgc aggcgcgggc       240
     ctatgcgatc ccgatgcggt gtactcgatc gtcgccgacg gctaccgagc ggttaccgat       300
     ttggtcggag ctggggcacg gttggatgaa tcggtcccgg gccgttgggc gttgacgcgc       360
     gaaggcgggc actcgcggcg acgcatcgtg cacgcgggtg gcgacgcgac cggcgccgag       420
     gttcagcggg cgctccagga tgccgccggg atgctcgata tccgcaccgg ccacgtggcg       480
     ttgcgagtgc tgcacgacgg taccgcggtg accgggctat tagtggtcag accggacgga       540
     tgcggcatta tcagcgctcc gtcggtgatc ctggccaccg gcgggctcgg gcacctgtac       600
     agcgcgacca ccaatccggc gggctccacc ggcgacggca tcgccctggg attgtgggcg       660
     ggcgtcgcgg tcagcgatct cgagttcatc cagttccacc ccacgatgct ttttgccgga       720
     cgcgccgggg gtcggcggcc gctgatcacc gaggccatcc gcggcgaggg tgcgatcttg       780
     gtggacaggc aaggcaattc gataacggca ggcgtgcatc cgatgggtga tttggcgccg       840
     cgcgacgtcg tcgccgccgc catcgacgcg cggctgaagg ccaccggcga tccgtgcgtc       900
     tacctcgacg cccgcggcat cgagggcttc gcgtcccggt tcccgacagt cacggcatcc       960
     tgccgggctg ccggcattga ccccgtccgg caaccgatcc cggttgttcc cggtgcgcac      1020
     tacagctgcg gcggcatagt gaccgatgtg tacggccaga ccgagctgct cgggttgtac      1080
     gccgctggcg aggtggcccg caccgggttg cacggcgcca accgcctggc ctccaacagc      1140
     ttgctagagg gtttggtggt gggcggccgc gccggaaagg ccgccgccgc ccacgccgcg      1200
     gcggccgggc gttcgcgtgc gacctcgtca gcgacctggc ccgaaccgat cagctacacc      1260
     gcactggacc gcggcgacct gcaacgggcg atgagccggg acgcgtcgat gtaccgcgcc      1320
     gccgccgggc tgcaccggct gtgcgacagc ctatccggag cacaggttcg cgacgtggct      1380
     tgtcgccgcg atttcgagga cgtggcgctc acgctggtcg cgcagagcgt gaccgccgcc      1440
     gccttggccc gcaccgaaag ccgtggctgc catcatcgcg cggagtaccc gtgcaccgtg      1500
     ccggagcagg cacgcagcat cgtggtccgg ggagccgacg acgcaaatgc ggtgtgtgtc      1560
     caggcgctag tggcggtgtg ctga                                             1584
//
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