spacer
spacer
EBIDatabasesDatabase BrowsingDbfetch

EBI Dbfetch

ID   CAB07079; SV 1; linear; genomic DNA; STD; PRO; 1290 BP.
XX
PA   BX842582.1
XX
DE   Mycobacterium tuberculosis H37Rv PROBABLE PHOSPHORIBOSYLAMINOIMIDAZOLE
DE   CARBOXYLASE ATPASE SUBUNIT PURK (AIR CARBOXYLASE) (AIRC)
XX
OS   Mycobacterium tuberculosis H37Rv
OC   Bacteria; Actinobacteria; Actinobacteridae; Actinomycetales;
OC   Corynebacterineae; Mycobacteriaceae; Mycobacterium;
OC   Mycobacterium tuberculosis complex.
OX   NCBI_TaxID=83332;
XX
FH   Key             Location/Qualifiers
FH
FT   source          1..1290
FT                   /organism="Mycobacterium tuberculosis H37Rv"
FT                   /strain="H37Rv"
FT                   /mol_type="genomic DNA"
FT   CDS             complement(BX842582.1:190972..192261)
FT                   /transl_table=11
FT                   /gene="purK"
FT                   /locus_tag="Rv3276c"
FT                   /product="PROBABLE PHOSPHORIBOSYLAMINOIMIDAZOLE CARBOXYLASE
FT                   ATPASE SUBUNIT PURK (AIR CARBOXYLASE) (AIRC)"
FT                   /function="INVOLVED IN PURINE BIOSYNTHESIS (SIXTH STEP).
FT                   POSSESSES AN ATPASE ACTIVITY THAT IS DEPENDENT ON THE
FT                   PRESENCE OF AIR (AMINOIMIDAZOLE RIBONUCLEOTIDE). THE
FT                   ASSOCIATION OF PURK AND PURE PRODUCES AN ENZYME COMPLEX
FT                   CAPABLE OF CONVERTING AIR TO CAIR EFFICIENTLY UNDER
FT                   PHYSIOLOGICAL CONDITION [CATALYTIC ACTIVITY:
FT                   1-(5-PHOSPHORIBOSYL)-5-AMINO-4-IMIDAZOLE-CARBOXYLATE =
FT                   1-(5-PHOSPHORIBOSYL)-5-AMINOIMIDAZOLE + CO(2)]."
FT                   /EC_number="4.1.1.21"
FT                   /note="Rv3276c, (MTCY71.16c), len: 429 aa. Probable purK,
FT                   phosphoribosylaminoimidazole carboxylase ATPase subunit (EC
FT                   4.1.1.21), equivalent to
FT                   P46701|PURK_MYCLE|ML0735|B1308_F1_32
FT                   PHOSPHORIBOSYLAMINOIMIDAZOLE CARBOXYLASE ATPASE SUBUNIT
FT                   from Mycobacterium leprae (439 aa), FASTA scores: opt:
FT                   2168, E(): 2.3e-123, (76.15% identity in 444 aa overlap).
FT                   Also similar to others e.g. Q44678|PURK_CORAM from
FT                   Corynebacterium ammoniagenes (Brevibacterium ammoniagenes)
FT                   (413 aa), FASTA scores: opt: 1179, E(): 9.1e-64, (48.35%
FT                   identity in 389 aa overlap); Q9KZ85|PURK from Streptomyces
FT                   coelicolor (368 aa), FASTA scores: opt: 1150, E(): 4.7e-62,
FT                   (55.35% identity in 345 aa overlap); Q54975|PURK_SYNP7 from
FT                   Synechococcus sp. strain PCC 7942 (Anacystis nidulans R2)
FT                   (395 aa), FASTA scores: opt: 772, E(): 3e-39, (38.1%
FT                   identity in 383 aa overlap); etc. BELONGS TO THE PURK /
FT                   PURT FAMILY."
FT                   /db_xref="GOA:P65898"
FT                   /db_xref="HSSP:1B6R"
FT                   /db_xref="InterPro:IPR013817"
FT                   /db_xref="UniProtKB/Swiss-Prot:P65898"
FT                   /func_characterised="identical sequence"
FT                   /protein_id="CAB07079.1"
FT                   /translation="MMAVASSRTPAVTSFIAPLVAMVGGGQLARMTHQAAIALGQNLRV
FT                   LVTSADDPAAQVTPNVVIGSHTDLAALRRVAAGADVLTFDHEHVPNELLEKLVADGVNV
FT                   APSPQALVHAQDKLVMRQRLAAAGVAVPRYAGIKDPDEIDVFAARVDAPIVVKAVRGGY
FT                   DGRGVRMARDVADARDFARECLADGVAVLVEERVDLRRELSALVARSPFGQGAAWPVVQ
FT                   TVQRDGTCVLVIAPAPALPDDLATAAQRLALQLADELGVVGVLAVELFETTDGALLVNE
FT                   LAMRPHNSGHWTIDGARTSQFEQHLRAVLDYPLGDSDAVVPVTVMANVLGAAQPPAMSV
FT                   DERLHHLFARMPDARVHLYGKAERPGRKVGHINFLGSDVAQLCERAELAAHWLSHGRWT
FT                   DGWDPHRASDDAVGVPPACGGRSDEEERRL"
XX
SQ   Sequence 1290 BP; 191 A; 419 C; 465 G; 215 T; 0 other; 3358342185 CRC32;
     atgatggccg tggcgagctc acgcaccccg gcggtcacct cgttcattgc ccccctcgtt        60
     gcgatggtcg gtggcggtca gctggcccgg atgacccatc aggctgccat cgcgctgggg       120
     cagaacctcc gtgtattggt cacctcggca gatgaccctg ccgcgcaggt cacccccaac       180
     gtggtgatcg gctcgcacac cgatcttgcc gccttgcgcc gcgttgcggc cggcgccgac       240
     gtgctgacct ttgaccacga gcacgtcccg aacgagctgt tggagaagct agtcgccgat       300
     ggcgtcaatg tcgcaccgtc gccgcaggcg ctggtgcacg cccaggacaa actcgtcatg       360
     cggcagcgtc tggctgcagc aggcgtcgcc gtgccccgct acgcgggcat caaagacccc       420
     gacgaaatcg acgtcttcgc cgcacgtgtc gacgccccga tcgtggtcaa ggcagtgcgc       480
     gggggttacg acggccgggg cgttcggatg gcacgtgatg tggcggatgc ccgtgatttc       540
     gctcgcgaat gcctggctga cggcgtggcg gtgctggtcg aggaacgggt ggacctgcgc       600
     cgcgagctgt cggcactggt ggcgcgttcg ccgttcggcc agggtgcggc gtggccggtg       660
     gtccagacgg tgcaacgtga cggcacgtgt gtgctggtga tcgcgccggc gccggcgctg       720
     cccgacgacc tggccaccgc ggcgcaacgg ttggcgttgc agctggccga cgaactcggt       780
     gtggtcggcg tgctcgccgt cgagctgttc gagacgaccg acggggcgtt gctggtcaac       840
     gagctcgcga tgcggccaca taattccggg cactggacca tcgacggggc gcgcaccagc       900
     cagttcgagc agcatctgcg cgcggtcttg gactacccgc tcggcgacag cgacgccgtg       960
     gtgcccgtga cggtgatggc caatgtgctc ggcgccgcgc agccgccggc catgagcgtc      1020
     gacgagcggc tgcaccatct gtttgcgcga atgcccgacg cccgggttca cctctacggc      1080
     aaggccgagc gacccggtcg caaggtgggg catatcaact tccttggctc cgatgtcgcc      1140
     cagttgtgtg aacgcgctga gctggcggca cactggttgt cccatgggcg gtggactgac      1200
     ggatgggacc cacaccgcgc cagcgacgat gcagtggggg taccacccgc gtgcggggga      1260
     cgaagcgatg aggaggagcg gcgcctatga                                       1290
//
spacer
spacer