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EBIDatabasesDatabase BrowsingDbfetch

EBI Dbfetch

ID   CAB07078; SV 1; linear; genomic DNA; STD; PRO; 525 BP.
XX
PA   BX842582.1
XX
DE   Mycobacterium tuberculosis H37Rv PROBABLE PHOSPHORIBOSYLAMINOIMIDAZOLE
DE   CARBOXYLASE CATALYTIC SUBUNIT PURE (AIR CARBOXYLASE) (AIRC)
XX
OS   Mycobacterium tuberculosis H37Rv
OC   Bacteria; Actinobacteria; Actinobacteridae; Actinomycetales;
OC   Corynebacterineae; Mycobacteriaceae; Mycobacterium;
OC   Mycobacterium tuberculosis complex.
OX   NCBI_TaxID=83332;
XX
FH   Key             Location/Qualifiers
FH
FT   source          1..525
FT                   /organism="Mycobacterium tuberculosis H37Rv"
FT                   /strain="H37Rv"
FT                   /mol_type="genomic DNA"
FT   CDS             complement(BX842582.1:190451..190975)
FT                   /transl_table=11
FT                   /gene="purE"
FT                   /locus_tag="Rv3275c"
FT                   /product="PROBABLE PHOSPHORIBOSYLAMINOIMIDAZOLE CARBOXYLASE
FT                   CATALYTIC SUBUNIT PURE (AIR CARBOXYLASE) (AIRC)"
FT                   /function="INVOLVED IN PURINE BIOSYNTHESIS (SIXTH STEP).
FT                   THIS SUBUNIT CAN ALONE TRANSFORM AIR TO CAIR, BUT IN
FT                   ASSOCIATION WITH PURK, WHICH POSSESSES AN ATPASE ACTIVITY,
FT                   AN ENZYME COMPLEX IS PRODUCED WHICH IS CAPABLE OF
FT                   CONVERTING AIR TO CAIR EFFICIENTLY UNDER PHYSIOLOGICAL
FT                   CONDITION [CATALYTIC ACTIVITY:
FT                   1-(5-PHOSPHORIBOSYL)-5-AMINO-4-IMIDAZOLE-CARBOXYLATE =
FT                   1-(5-PHOSPHORIBOSYL)-5-AMINOIMIDAZOLE + CO(2)]."
FT                   /EC_number="4.1.1.21"
FT                   /note="Rv3275c, (MTCY71.15c, PUR6), len: 174 aa. Probable
FT                   purE, phosphoribosylaminoimidazole carboxylase catalytic
FT                   subunit (EC 4.1.1.21), equivalent to
FT                   P46702|PUR6_MYCLE|PURE|ML0736|B1308_F3_98 from
FT                   Mycobacterium leprae (171 aa), FASTA scores: opt: 878, E():
FT                   1.5e-43, (81.55% identity in 168 aa overlap). Also similar
FT                   to others e.g. Q9AXD0|AIRC from Nicotiana tabacum (Common
FT                   tobacco) (623 aa), FASTA scores: opt: 712, E(): 1.4e-33,
FT                   (69.35% identity in 160 aa overlap) (similarity in
FT                   C-terminal part for this one); Q44679|PUR6_CORAM from
FT                   Corynebacterium ammoniagenes (Brevibacterium ammoniagenes)
FT                   (177 aa), FASTA scores: opt: 651, E(): 1.5e-30, (68.25%
FT                   identity in 148 aa overlap); Q55498|PUR6_SYNY3|PURE|SLL0901
FT                   from Synechocystis sp. strain PCC 6803 (176 aa), FASTA
FT                   scores: opt: 639, E(): 7.1e-30, (60.5% identity in 167 aa
FT                   overlap); etc."
FT                   /db_xref="GOA:P96880"
FT                   /db_xref="HSSP:1O4V"
FT                   /db_xref="InterPro:IPR000031"
FT                   /db_xref="UniProtKB/Swiss-Prot:P96880"
FT                   /experiment="experimental evidence, no additional details
FT                   recorded"
FT                   /func_characterised="identical sequence"
FT                   /protein_id="CAB07078.1"
FT                   /translation="MTPAGERPRVGVIMGSDSDWPVMADAAAALAEFDIPAEVRVVSAH
FT                   RTPEAMFSYARGAAERGLEVIIAGAGGAAHLPGMVAAATPLPVIGVPVPLGRLDGLDSL
FT                   LSIVQMPAGVPVATVSIGGAGNAGLLAVRMLGAANPQLRARIVAFQDRLADVVAAKDAE
FT                   LQRLAGKLTRD"
XX
SQ   Sequence 525 BP; 66 A; 166 C; 207 G; 86 T; 0 other; 189416200 CRC32;
     atgactcctg ccggcgagcg gccccgggtc ggggtgatca tgggcagcga cagcgactgg        60
     ccggtgatgg ccgacgctgc ggcggcgctg gccgagttcg acattccggc cgaggttcgg       120
     gttgtttcgg cccatcgcac ccctgaggcg atgttcagtt atgcgcgcgg cgcggccgag       180
     cgcggtctcg aggtgatcat cgccggagcg ggcggggccg cgcacttgcc cggtatggtc       240
     gccgccgcga cgccgctgcc ggtgatcggg gtgccggtac cgctgggcag gctggacggc       300
     cttgactccc tgctgtcgat cgtgcaaatg ccggccgggg ttccggtggc cacggtgtcc       360
     atcgggggcg ccggtaatgc cggactgctg gcggtgcgga tgctgggagc cgccaacccc       420
     cagctgaggg cccggatcgt cgcattccag gaccggctgg ccgacgtcgt ggcggccaag       480
     gatgcggaac tgcagcggct tgcgggtaag ttaacccgcg actag                       525
//
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