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EBIDatabasesDatabase BrowsingDbfetch

EBI Dbfetch

ID   CAB03733; SV 1; linear; genomic DNA; STD; PRO; 765 BP.
XX
PA   BX842579.1
XX
DE   Mycobacterium tuberculosis H37Rv PROBABLE 3'-PHOSPHOADENOSINE
DE   5'-PHOSPHOSULFATE REDUCTASE CYSH (PAPS REDUCTASE, THIOREDOXIN DEP.) (PADOPS
DE   REDUCTASE) (3'-PHOSPHOADENYLYLSULFATE REDUCTASE) (PAPS SULFOTRANSFERASE)
XX
OS   Mycobacterium tuberculosis H37Rv
OC   Bacteria; Actinobacteria; Actinobacteridae; Actinomycetales;
OC   Corynebacterineae; Mycobacteriaceae; Mycobacterium;
OC   Mycobacterium tuberculosis complex.
OX   NCBI_TaxID=83332;
XX
FH   Key             Location/Qualifiers
FH
FT   source          1..765
FT                   /organism="Mycobacterium tuberculosis H37Rv"
FT                   /strain="H37Rv"
FT                   /mol_type="genomic DNA"
FT   CDS             BX842579.1:261528..262292
FT                   /transl_table=11
FT                   /gene="cysH"
FT                   /locus_tag="Rv2392"
FT                   /product="PROBABLE 3'-PHOSPHOADENOSINE 5'-PHOSPHOSULFATE
FT                   REDUCTASE CYSH (PAPS REDUCTASE, THIOREDOXIN DEP.) (PADOPS
FT                   REDUCTASE) (3'-PHOSPHOADENYLYLSULFATE REDUCTASE) (PAPS
FT                   SULFOTRANSFERASE)"
FT                   /function="INVOLVED IN THE SULFATE ACTIVATION PATHWAY (AT
FT                   THE THIRD STEP) IN THE REDUCTIVE BRANCH OF THE CYSTEINE
FT                   BIOSYNTHETIC PATHWAY. REDUCES ACTIVATED SULFATE INTO
FT                   SULFITE [CATALYTIC ACTIVITY: 5-PHOSPHOADENOSINE
FT                   3-PHOSPHOSULFATE + REDUCED THIOREDOXIN = PHOSPHOADENOSINE
FT                   PHOSPHATE + OXIDIZED THIOREDOXIN + SULFITE]."
FT                   /EC_number="1.8.4.8"
FT                   /note="Rv2392, (MTCY253.29c), len: 254 aa. Probable cysH,
FT                   3'-phosphoadenosine 5'-phosphosulfate reductase (EC
FT                   1.8.99.4) (see citation below), similar to many e.g.
FT                   P94498|O34620|CYH1_BACSU|CYSH from Bacillus subtilis (233
FT                   aa), FASTA scores: opt: 618, E(): 8.1e-32, (46.5% identity
FT                   in 202 aa overlap); Q9KCT3|CYSH|BH1486 from Bacillus
FT                   halodurans (231 aa), FASTA scores: opt: 560, E(): 3.6e-28,
FT                   (41.3% identity in 230 aa overlap); P56860|CYSH_DEIRA from
FT                   Deinococcus radiodurans (255 aa), FASTA scores: opt: 489,
FT                   E(): 1.1e-23, (44.7% identity in 190 aa overlap); etc.
FT                   BELONGS TO THE PAPS REDUCTASE FAMILY and CYSH SUBFAMILY.
FT                   Note that operon cysA-cysW-cysT-subI, probably involved in
FT                   sulfate transport, is near this putative ORF."
FT                   /db_xref="GOA:P65668"
FT                   /db_xref="InterPro:IPR014729"
FT                   /db_xref="UniProtKB/Swiss-Prot:P65668"
FT                   /func_characterised="identical sequence"
FT                   /protein_id="CAB03733.1"
FT                   /translation="MSGETTRLTEPQLRELAARGAAELDGATATDMLRWTDETFGDIGG
FT                   AGGGVSGHRGWTTCNYVVASNMADAVLVDLAAKVRPGVPVIFLDTGYHFVETIGTRDAI
FT                   ESVYDVRVLNVTPEHTVAEQDELLGKDLFARNPHECCRLRKVVPLGKTLRGYSAWVTGL
FT                   RRVDAPTRANAPLVSFDETFKLVKVNPLAAWTDQDVQEYIADNDVLVNPLVREGYPSIG
FT                   CAPCTAKPAEGADPRSGRWQGLAKTECGLHAS"
XX
SQ   Sequence 765 BP; 149 A; 239 C; 252 G; 125 T; 0 other; 1027560072 CRC32;
     atgagcggcg agacaaccag gctgaccgaa ccgcaactac gtgagctggc cgcgcgcgga        60
     gctgccgaac tcgacggcgc caccgccacc gacatgttgc gctggaccga cgaaaccttc       120
     ggcgacatcg gcggcgccgg cggcggcgtg agcggacatc gcgggtggac aacgtgcaac       180
     tacgtagttg cttccaacat ggctgatgcg gtgctggtgg atctggccgc caaggtgcga       240
     ccgggcgtac cggtcatctt tcttgatacc ggctaccact tcgtcgaaac aatcggcacc       300
     agagatgcga tcgagtccgt ctatgacgtc cgggtgctca atgtcactcc ggagcacaca       360
     gtggccgagc aggacgaact gctgggcaag gacttgttcg cccgcaaccc ccatgaatgc       420
     tgccggttgc gcaaggtcgt tcccctgggc aagacgctgc gtggctactc cgcgtgggtg       480
     accgggctac ggcgggtcga tgcaccgacc cgggccaatg ccccgctggt cagcttcgat       540
     gagacgttca aactagtgaa ggtcaacccg ctggcggcgt ggaccgacca agatgtgcag       600
     gaatacattg ccgacaacga cgtgctggtt aatccgcttg tgcgggaagg ctatccgtcg       660
     atcggttgcg ctccgtgcac agccaaaccc gccgaaggcg ccgacccgcg cagcggacgc       720
     tggcaggggc tggccaagac cgaatgcggg ttgcacgcct cgtga                       765
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