EBI Dbfetch

ID   CAB02059; SV 2; linear; genomic DNA; STD; PRO; 1647 BP.
XX
PA   BX842579.1
XX
DE   Mycobacterium tuberculosis H37Rv PROBABLE [NAD] DEPENDENT MALATE
DE   OXIDOREDUCTASE MEZ (MALIC ENZYME) (NAD-MALIC ENZYME) (MALATE DEHYDROGENASE
DE   (OXALOACETATE DECARBOXYLATING)) (PYRUVIC-MALIC CARBOXYLASE) (NAD-ME)
XX
OS   Mycobacterium tuberculosis H37Rv
OC   Bacteria; Actinobacteria; Actinobacteridae; Actinomycetales;
OC   Corynebacterineae; Mycobacteriaceae; Mycobacterium;
OC   Mycobacterium tuberculosis complex.
OX   NCBI_TaxID=83332;
XX
FH   Key             Location/Qualifiers
FH
FT   source          1..1647
FT                   /organism="Mycobacterium tuberculosis H37Rv"
FT                   /strain="H37Rv"
FT                   /mol_type="genomic DNA"
FT   CDS             BX842579.1:180269..181915
FT                   /transl_table=11
FT                   /gene="mez"
FT                   /locus_tag="Rv2332"
FT                   /product="PROBABLE [NAD] DEPENDENT MALATE OXIDOREDUCTASE
FT                   MEZ (MALIC ENZYME) (NAD-MALIC ENZYME) (MALATE DEHYDROGENASE
FT                   (OXALOACETATE DECARBOXYLATING)) (PYRUVIC-MALIC CARBOXYLASE)
FT                   (NAD-ME)"
FT                   /function="CATALIZES THE OXIDATIVE DECARBOXYLATION OF
FT                   MALATE INTO PYRUVATE, IMPORTANT FOR A WIDE RANGE OF
FT                   METABOLIC PATHWAYS [CATALYTIC ACTIVITY: (S)-MALATE + NAD(+)
FT                   = PYRUVATE + CO(2) + NADH]."
FT                   /EC_number="1.1.1.38"
FT                   /note="Rv2332, (MTCY3G12.02c, MTCY98.01, MT2394), len: 548
FT                   aa. Probable mez, malate oxidoreductase [NAD] dependent (EC
FT                   1.1.1.38) (malic enzyme), highly similar to others e.g.
FT                   O34389|MALS PUTATIVE MALOLACTIC ENZYME [INCLUDES: MALIC
FT                   ENZYME (EC 1.1.1.-); L-LACTATE DEHYDROGENASE (EC 1.1.1.27)]
FT                   from Bacillus subtilis (566 aa), FASTA scores: opt: 1927,
FT                   E(): 5.5e-111, (52.9% identity in 539 aa overlap);
FT                   P45868|MAO2_BACSU|YWKA PROBABLE NAD-DEPENDENT MALIC ENZYME
FT                   from Bacillus subtilis (582 aa), FASTA scores: opt: 1849,
FT                   E(): 3.6e-106, (50.45% identity in 543 aa overlap);
FT                   Q48796|MLES_OENOE MALOLACTIC ENZYME from Oenococcus oeni
FT                   (541 aa), FASTA scores: opt: 1540, E(): 3.6e-87, (44.2%
FT                   identity in 536 aa overlap); etc. BELONGS TO THE MALIC
FT                   ENZYMES FAMILY. N-terminus shortened since first submission
FT                   (previously 652 aa)."
FT                   /db_xref="GOA:P71880"
FT                   /db_xref="HSSP:1LLQ"
FT                   /db_xref="InterPro:IPR016040"
FT                   /db_xref="UniProtKB/Swiss-Prot:P71880"
FT                   /func_characterised="identical sequence"
FT                   /protein_id="CAB02059.2"
FT                   /translation="MSDARVPRIPAALSAPSLNRGVGFTHAQRRRLGLTGRLPSAVLTL
FT                   DQQAERVWHQLQSLATELGRNLLLEQLHYRHEVLYFKVLADHLPELMPVVYTPTVGEAI
FT                   QRFSDEYRGQRGLFLSIDEPDEIEEAFNTLGLGPEDVDLIVCTDAEAILGIGDWGVGGI
FT                   QIAVGKLALYTAGGGVDPRRCLAVSLDVGTDNEQLLADPFYLGNRHARRRGREYDEFVS
FT                   RYIETAQRLFPRAILHFEDFGPANARKILDTYGTDYCVFNDDMQGTGAVVLAAVYSGLK
FT                   VTGIPLRDQTIVVFGAGTAGMGIADQIRDAMVADGATLEQAVSQIWPIDRPGLLFDDMD
FT                   DLRDFQVPYAKNRHQLGVAVGDRVGLSDAIKIASPTILLGCSTVYGAFTKEVVEAMTAS
FT                   CKHPMIFPLSNPTSRMEAIPADVLAWSNGRALLATGSPVAPVEFDETTYVIGQANNVLA
FT                   FPGIGLGVIVAGARLITRRMLHAAAKAIAHQANPTNPGDSLLPDVQNLRAISTTVAEAV
FT                   YRAAVQDGVASRTHDDVRQAIVDTMWLPAYD"
XX
SQ   Sequence 1647 BP; 309 A; 521 C; 524 G; 293 T; 0 other; 3212264753 CRC32;
     atgagcgacg cccgcgtgcc acggatcccg gccgcgttgt ccgcaccaag tctcaaccgt        60
     ggagtcggct tcacccacgc gcagcggcgg cggctggggc tgaccggccg gcttccgtcg       120
     gccgtgctca cgctcgacca acaggccgaa cgcgtatggc atcagttgca gagcttggcc       180
     accgagctgg gccgcaacct gcttctcgaa cagctgcact accgccacga ggtgctgtac       240
     ttcaaggtgc tggccgacca tttgcccgaa ctgatgccgg tggtgtacac gcccaccgtt       300
     ggcgaggcaa tccaacgctt ctccgacgaa taccgcgggc aacgcggact gtttctgagc       360
     atcgacgaac ccgacgaaat cgaggaagcc ttcaacacgt tggggctggg gcccgaggac       420
     gtcgacctga tcgtgtgcac cgatgccgag gcgatcctgg gtatcggtga ctggggtgtg       480
     ggtggcatcc agatcgctgt gggcaaattg gccctctaca ccgccggcgg cggcgtcgat       540
     ccgcgccgct gcctcgcggt gtctctggat gtcggcaccg acaatgagca gctgctggcc       600
     gatccgttct atctgggcaa tcgccacgcc cggcggcgcg gtcgggaata cgacgagttc       660
     gtcagtcgct atatcgaaac ggctcaacgg ttatttccgc gtgccattct gcatttcgag       720
     gacttcgggc cggcgaacgc gcggaagatc ctagacacat acggcacgga ttactgcgtg       780
     ttcaacgatg acatgcaagg aaccggcgcg gtggtcttgg ccgccgtata cagcggtctg       840
     aaggttaccg gtatcccgct gcgcgatcag acaatagtcg tcttcggcgc aggcaccgca       900
     gggatgggga tcgccgatca gatccgggac gcgatggtgg cagacggtgc cacgctcgag       960
     caggcggtgt cccagatctg gccgatcgac aggccgggcc tgttgttcga cgacatggat      1020
     gacctgcgcg acttccaagt gccgtacgcg aaaaaccgcc accagctcgg tgtggccgtc      1080
     ggggatcggg tcgggctgag cgacgcgatc aagatcgcat cgcccactat cctgctcggc      1140
     tgctcaacgg tctacggagc gttcaccaaa gaggtggtcg aggcgatgac ggcgtcctgc      1200
     aaacacccga tgatctttcc gctgtccaac ccgacgtcgc gcatggaagc catccccgcc      1260
     gacgtgctgg cgtggtcgaa tggcagggcg ctgcttgcca ccggcagccc agtcgcccca      1320
     gtggaattcg acgaaaccac ctacgtcatc ggtcaggcca acaacgtgtt ggcgtttccc      1380
     ggcatcggac tgggcgtcat tgtcgctggt gcccggttga taaccaggcg catgctgcat      1440
     gcagcagcga aggccattgc gcaccaggcc aatccgacaa atcccggaga ctcgctgttg      1500
     ccggatgtcc aaaatctgcg ggccatctcg acaacggtcg ccgaagctgt ctatcgggcc      1560
     gccgtccaag acggggtggc ttccaggacg cacgacgacg tcaggcaggc catagtcgac      1620
     accatgtggc tcccggcata tgactaa                                          1647
//