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EBIDatabasesDatabase BrowsingDbfetch

EBI Dbfetch

ID   CAA98341; SV 1; linear; genomic DNA; STD; PRO; 885 BP.
XX
PA   BX842581.1
XX
DE   Mycobacterium tuberculosis H37Rv PROBABLE SIGNAL PEPTIDASE I LEPB (SPASE I)
DE   (LEADER PEPTIDASE I).
XX
OS   Mycobacterium tuberculosis H37Rv
OC   Bacteria; Actinobacteria; Actinobacteridae; Actinomycetales;
OC   Corynebacterineae; Mycobacteriaceae; Mycobacterium;
OC   Mycobacterium tuberculosis complex.
OX   NCBI_TaxID=83332;
XX
FH   Key             Location/Qualifiers
FH
FT   source          1..885
FT                   /organism="Mycobacterium tuberculosis H37Rv"
FT                   /strain="H37Rv"
FT                   /mol_type="genomic DNA"
FT   CDS             complement(BX842581.1:93933..94817)
FT                   /transl_table=11
FT                   /gene="lepB"
FT                   /locus_tag="Rv2903c"
FT                   /product="PROBABLE SIGNAL PEPTIDASE I LEPB (SPASE I)
FT                   (LEADER PEPTIDASE I)."
FT                   /function="CLEAVAGE OF N-TERMINAL LEADER SEQUENCES FROM
FT                   SECRETED PROTEIN PRECURSORS."
FT                   /EC_number="3.4.21.89"
FT                   /note="Rv2903c, (MTCY274.34c), len: 294 aa. Probable lepB,
FT                   signal peptidase I (EC 3.4.21.89) (TYPE II MEMBRANE
FT                   PROTEIN) (see Braunstein & Belisle 2000), equivalent to
FT                   O33021|LEP_MYCLE|ML1612|MLCB250.39 PROBABLE SIGNAL
FT                   PEPTIDASE I from Mycobacterium leprae (289 aa), FASTA
FT                   scores: opt: 1335, E(): 1.8e-77, (69.75% identity in 301 aa
FT                   overlap). Also similar to many e.g. O86869|SIPX SIGNAL
FT                   PEPTIDASE I from Streptomyces lividans (320 aa), FASTA
FT                   scores: opt: 474, E(): 1e-22, (43.55% identity in 248 aa
FT                   overlap); O69884|SIP1|SIPW PUTATIVE SIGNAL PEPTIDASE I from
FT                   Streptomyces coelicolor and Streptomyces lividans (259 aa),
FT                   FASTA scores: opt: 226, E(): 5e-07, (36.0% identity in 214
FT                   aa overlap); P42668|LEP_BACLI|SIP SIGNAL PEPTIDASE I from
FT                   Bacillus licheniformis (186 aa), FASTA scores: opt: 218,
FT                   E(): 1.3e-06, (34.5% identity in 194 aa overlap); etc.
FT                   Contains PS00501 Signal peptidases I serine active site,and
FT                   PS00761 Signal peptidases I signature 3. BELONGS TO
FT                   PEPTIDASE FAMILY S26; ALSO KNOWN AS TYPE I LEADER PEPTIDASE
FT                   FAMILY."
FT                   /db_xref="GOA:Q10789"
FT                   /db_xref="HSSP:1B12"
FT                   /db_xref="InterPro:IPR011056"
FT                   /db_xref="UniProtKB/Swiss-Prot:Q10789"
FT                   /experiment="experimental evidence, no additional details
FT                   recorded"
FT                   /func_characterised="identical sequence"
FT                   /protein_id="CAA98341.1"
FT                   /translation="MTETTDSPSERQPGPAEPELSSRDPDIAGQVFDAAPFDAAPDADS
FT                   EGDSKAAKTDEPRPAKRSTLREFAVLAVIAVVLYYVMLTFVARPYLIPSESMEPTLHGC
FT                   STCVGDRIMVDKLSYRFGSPQPGDVIVFRGPPSWNVGYKSIRSHNVAVRWVQNALSFIG
FT                   FVPPDENDLVKRVIAVGGQTVQCRSDTGLTVNGRPLKEPYLDPATMMADPSIYPCLGSE
FT                   FGPVTVPPGRVWVMGDNRTHSADSRAHCPLLCTDDPLPGTVPVANVIGKARLIVWPPSR
FT                   WGVVRSVNPQQGR"
XX
SQ   Sequence 885 BP; 146 A; 287 C; 289 G; 163 T; 0 other; 116658005 CRC32;
     gtgaccgaaa ccacggactc cccatcggag cgccagccgg gtccggcaga gccggagctc        60
     tcctcccggg acccggacat tgccggccag gtcttcgacg cagccccgtt cgacgcagcc       120
     ccggatgcgg actccgaagg cgactccaag gcggccaaaa cggacgagcc gcggcccgcg       180
     aagcgatcaa cgctgcggga gttcgcggtg ctggcggtga ttgctgtggt gctctactac       240
     gtcatgctga cgtttgtcgc gcgcccttat ctgattccgt cggaatcgat ggaacccacg       300
     ttgcacgggt gttcgacgtg cgtcggcgac cgcatcatgg tggacaaact cagctaccgc       360
     ttcggctcac cgcaacctgg cgacgtcatc gtcttcaggg gaccgccgtc gtggaacgtt       420
     ggttacaagt cgatccgttc gcacaacgtc gccgtgcgct gggtgcagaa cgcgttgtcg       480
     ttcatcggtt tcgtgcctcc cgacgagaac gacctggtca agcgtgtcat cgcggtcggc       540
     ggacagacgg ttcaatgccg gtccgacacc ggcctgacgg tcaacggcag gccactgaag       600
     gagccatacc tggatccggc caccatgatg gccgacccgt cgatataccc gtgcctgggc       660
     agcgagttcg ggccggtcac cgtcccgccc gggcgtgtct gggtgatggg cgacaaccgc       720
     acccattcgg cggattcccg cgctcactgc ccgttgctat gtactgacga tccgctaccg       780
     gggaccgtgc cggtggccaa cgtcatcggt aaggccaggt tgatcgtgtg gccgccgtcg       840
     cgttggggtg ttgtgcgttc ggtgaatccc cagcaaggtc ggtag                       885
//
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