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EBIDatabasesDatabase BrowsingDbfetch

EBI Dbfetch

ID   CAA97751; SV 1; linear; genomic DNA; STD; PRO; 999 BP.
XX
PA   BX842576.1
XX
DE   Mycobacterium tuberculosis H37Rv PROBABLE SULFATE ADENYLYLTRANSFERASE
DE   SUBUNIT 2 CYSD
XX
OS   Mycobacterium tuberculosis H37Rv
OC   Bacteria; Actinobacteria; Actinobacteridae; Actinomycetales;
OC   Corynebacterineae; Mycobacteriaceae; Mycobacterium;
OC   Mycobacterium tuberculosis complex.
OX   NCBI_TaxID=83332;
XX
FH   Key             Location/Qualifiers
FH
FT   source          1..999
FT                   /organism="Mycobacterium tuberculosis H37Rv"
FT                   /strain="H37Rv"
FT                   /mol_type="genomic DNA"
FT   CDS             BX842576.1:54866..55864
FT                   /transl_table=11
FT                   /gene="cysD"
FT                   /locus_tag="Rv1285"
FT                   /product="PROBABLE SULFATE ADENYLYLTRANSFERASE SUBUNIT 2
FT                   CYSD"
FT                   /function="INVOLVED IN SULFATE ACTIVATION PATHWAY. FIRST
FT                   STEP IN THE SULFATE ACTIVATION PATHWAY. THIS REACTION
FT                   OCCURS EARLY IN THE REDUCTIVE BRANCH OF THE CYSTEINE
FT                   BIOSYNTHETIC PATHWAY [CATALYTIC ACTIVITY :ATP + SULFATE =
FT                   DIPHOSPHATE + ADENYLYLSULFATE]"
FT                   /EC_number="2.7.7.4"
FT                   /note="Rv1285, (MTCY373.04), len: 332 aa. Probable cysD,
FT                   sulfate adenylyltransferase subunit 2 (EC 2.7.7.4) (see
FT                   Wooff et al., 2002), homology suggests start site at aa 24
FT                   or 28, similar to e.g. CYSD_ECOLI|P21156 sulfate adenylate
FT                   transferase subunit 2 from Escherichia coli (302 aa), FASTA
FT                   score: opt: 973, E():0, (52.5% identity in 303 aa overlap).
FT                   Also similar to Mycobacterium tuberculosis Rv2392,
FT                   3'-phosphoadenylylsulfate reductase. BELONGS TO THE PAPS
FT                   REDUCTASE FAMILY. CYSD SUBFAMILY. Thought to be
FT                   differentially expressed within host cells (see Triccas et
FT                   al., 1999)."
FT                   /db_xref="GOA:P65670"
FT                   /db_xref="InterPro:IPR011784"
FT                   /db_xref="UniProtKB/Swiss-Prot:P65670"
FT                   /experiment="experimental evidence, no additional details
FT                   recorded"
FT                   /func_characterised="similar sequence"
FT                   /protein_id="CAA97751.1"
FT                   /translation="MAITINMVNPTGFIRYEDVEQEAMTSDVTVGPAPGQYQLSHLRLL
FT                   EAEAIHVIREVAAEFERPVLLFSGGKDSIVMLHLALKAFRPGRLPFPVMHVDTGHNFDE
FT                   VIATRDELVAAAGVRLVVASVQDDIDAGRVVETIPSRNPIQTVTLLRAIRENQFDAAFG
FT                   GARRDEEKARAKERVFSFRDEFGQWDPKAQRPELWNLYNGRHHKGEHIRVFPLSNWTEF
FT                   DIWSYIGAEQVRLPSIYFAHRRKVFQRDGMLLAVHRHMQPRADEPVFEATVRFRTVGDV
FT                   TCTGCVESSASTVAEVIAETAVARLTERGATRADDRISEAGMEDRKRQGYF"
XX
SQ   Sequence 999 BP; 189 A; 303 C; 341 G; 166 T; 0 other; 1868390430 CRC32;
     atggcaataa ccataaatat ggtcaatcct accggattta tcaggtatga ggacgtggaa        60
     caggaagcca tgaccagcga tgtgacggtg ggccccgcac ccggccagta ccaactgagc       120
     catctgcgct tgctggaggc cgaagccatc cacgtcatcc gggaggtggc cgccgagttc       180
     gagcggccag tgctgttgtt ctcggggggc aaggactcca tcgtcatgct gcacctggcg       240
     ctgaaggcgt ttcggcccgg gcgactgccg ttcccggtca tgcacgtcga caccggtcac       300
     aacttcgacg aagttatcgc tacccgagac gagttggtcg ccgcggccgg ggtgcggctg       360
     gtggtggcgt cggtgcagga cgatatcgat gccggtcggg tcgtcgagac catcccgtcg       420
     cgaaatccga tacagaccgt gacgctgctg cgggccatcc gggagaacca attcgacgcg       480
     gcattcgggg gagcccggcg cgacgaggag aaggcccgcg ccaaggagcg ggtgttcagc       540
     ttccgcgacg agttcggcca gtgggacccg aaggctcagc ggccggaact gtggaacctc       600
     tacaacggac ggcaccacaa gggcgagcac atccgggtct tcccgctgtc caactggacc       660
     gaattcgaca tctggtccta catcggcgcc gagcaggtca ggctgccgtc catctatttc       720
     gcccaccggc gcaaggtgtt tcagcgcgac ggcatgttgc tggccgtgca ccggcacatg       780
     caaccgcgag ccgacgagcc ggtgttcgag gccacggtgc gattccgcac cgtcggggat       840
     gttacctgca ccgggtgcgt cgagtcgtcg gcatcgacgg tcgcggaagt catcgccgaa       900
     actgcggtgg cccgcttgac ggagcgcggg gcgaccaggg ctgacgaccg gatctcggag       960
     gctggaatgg aagaccgcaa gcggcaggga tacttctga                              999
//
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