EBI Dbfetch

ID   CAA90423; SV 1; linear; mRNA; STD; FUN; 1320 BP.
XX
PA   Z50095.1
XX
DE   Agaricus bisporus CEL4b mannanase
XX
OS   Agaricus bisporus
OC   Eukaryota; Fungi; Dikarya; Basidiomycota; Agaricomycotina; Agaricomycetes;
OC   Agaricomycetidae; Agaricales; Agaricaceae; Agaricus.
OX   NCBI_TaxID=5341;
XX
FH   Key             Location/Qualifiers
FH
FT   source          1..1320
FT                   /organism="Agaricus bisporus"
FT                   /strain="D649"
FT                   /mol_type="mRNA"
FT                   /dev_stage="young mycelia"
FT                   /clone_lib="lambda ZAP"
FT                   /clone="pEYc1200"
FT                   /tissue_type="mycelium"
FT   CDS             Z50095.1:6..1325
FT                   /gene="cel4"
FT                   /standard_name="beta-mannanase"
FT                   /product="CEL4b mannanase"
FT                   /function="endo-fashion hydrolysis of mannan and
FT                   heteromannan (by sequence comparison; no experimental
FT                   evidence)"
FT                   /EC_number="3.2.1.68"
FT                   /note="cellulose binding domain: 63..170"
FT                   /note="Expression of the gene induced (Northern analysis)
FT                   by crystalline cellulose. Repressed (Northern) by glucose,
FT                   fructose, lactose."
FT                   /note="Glycerol, mannitol, sorbitol, and maltose are
FT                   neutral carbon sources (Northern)"
FT                   /note="Catalytic domain (261..1325) belongs to family 5 of
FT                   glycosyl hydrolases (cellulase family A)"
FT                   /db_xref="GOA:Q92401"
FT                   /db_xref="HSSP:1QNR"
FT                   /db_xref="InterPro:IPR001547"
FT                   /db_xref="UniProtKB/TrEMBL:Q92401"
FT                   /protein_id="CAA90423.1"
FT                   /translation="MKPAIRFIILAISISLATADVPVWGQCGGRDWTGETACASGSSCV
FT                   VQNEWYSQCLPGSTTPTNPPPATTTSQTTAPPTTSHPVSTGFVKASGTRFTLNGQKYTV
FT                   VGGNSYWVGLTGLSTSAMNQAFSDIANAGGTTVRTWGFNEVTSPNGNYYQSWSGARPTI
FT                   NTGASGLLNFDNVIAAAKANGIRLIVALTNNWADYGGMDVYVNQMVGNGQPHDLFYTNT
FT                   AIKDAFKSYGRAFVSRYANEPTVMAWELANEPRCKGSTGTTSGTCTTTTVTNWAKEMSA
FT                   FIKTIDSNHLVAIGDEGFYNQPGAPTYPYQGSEGVDFEANLAISSVDFATFHSYPEPWG
FT                   QGADAKAWGTQWITDHAASMKRVNKPVILEEFGVTTNQPDTYAEWFNEIESSGLTGDLI
FT                   WQAGSHLSTGDTPNDGYAVYPDGPVYPLVKSHASAMKNRA"
XX
SQ   Sequence 1320 BP; 340 A; 337 C; 320 G; 323 T; 0 other; 445062959 CRC32;
     atgaagcctg cgattagatt cattattttg gctatttcta tctctctagc gaccgcagat        60
     gttccagtct ggggtcagtg cggtggcagg gattggacgg gagagacagc ttgtgcaagt       120
     ggatcatctt gtgtcgtgca gaacgaatgg tactcgcagt gcctccctgg atcaacaact       180
     ccaacaaacc ctccccctgc cactaccacc tcccaaacta ccgctcctcc taccacttca       240
     cacccagtgt cgaccggatt tgtcaaagct tctggtacca gattcactct aaatggccaa       300
     aaatacaccg ttgttggagg caattcctac tgggtcggtt taactgggct gagcaccagt       360
     gcaatgaatc aggctttctc cgacatcgct aacgctggag gaacaaccgt ccgaacatgg       420
     ggattcaatg aagtaacctc gccaaacggc aattattatc aatcctggtc tggtgccaga       480
     cctactataa acaccggagc gtctggtcta cttaatttcg acaatgtgat tgctgcggcc       540
     aaggcaaatg gaattcgtct gatagttgct ctgacgaata attgggcgga ctatggtggc       600
     atggacgtct atgtcaacca gatggtgggc aatggccaac cacacgatct tttctacaca       660
     aacaccgcaa tcaaggacgc gttcaaatct tatggccggg ccttcgtgag ccgctatgcc       720
     aacgaaccta ccgtaatggc ttgggagctt gcaaatgagc cgaggtgcaa aggtagcacc       780
     ggcacgactt ctggaacctg tacaactacg accgtcacga attgggccaa ggagatgtcg       840
     gcgtttatta aaaccattga tagcaaccat ctcgttgcta ttggtgatga gggtttctat       900
     aatcaacccg gtgctccaac ttatccatac caaggaagcg aaggtgttga tttcgaagct       960
     aatctcgcta tctcctctgt cgactttgcg accttccatt cctatccgga accgtgggga      1020
     cagggcgccg atgcaaaagc ctggggcact caatggatta cagatcatgc tgccagtatg      1080
     aagagggtca acaaacccgt tatccttgaa gaattcggtg taactactaa tcaacctgat      1140
     acttacgctg agtggttcaa cgagatagaa tcatcagggt tgactggtga tctcatttgg      1200
     caagccggtt ctcacttgtc tacgggagat acacccaatg acggttatgc tgtctacccc      1260
     gatggtccag tatacccgtt agtgaagtcg catgcaagtg caatgaaaaa ccgggcttga      1320
//