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EBI DbfetchID CAA80815; SV 1; linear; genomic DNA; STD; PRO; 7104 BP. XX PA Z23277.1 XX DE Clostridium difficile toxin B XX OS Clostridium difficile OC Bacteria; Firmicutes; Clostridia; Clostridiales; Clostridiaceae; OC Clostridium. OX NCBI_TaxID=1496; XX FH Key Location/Qualifiers FH FT source 1..7104 FT /organism="Clostridium difficile" FT /isolate="isolate 1470" FT /mol_type="genomic DNA" FT /clone_lib="genomic DNA in pUC18" FT /clone="pTBF1, pTBF3, pTBF4, pTBF5, pTBF6" FT CDS Z23277.1:61..7164 FT /transl_table=11 FT /product="toxin B" FT /db_xref="HSSP:1GVM" FT /db_xref="InterPro:IPR018337" FT /db_xref="UniProtKB/TrEMBL:Q46034" FT /protein_id="CAA80815.1" FT /translation="MSLVNRKQLEKMANVRFRVQEDEYVAILDALEEYHNMSENTVVEK FT YLKLKDINSLTDTYIDTYKKSGRNKALKKFKEYLVIEILELKNSNLTPVEKNLHFIWIG FT GQINDTAINYINQWKDVNSDYNVNVFYDSNAFLINTLKKTIIESASNDTLESFRENLND FT PEFNHTAFFRKRMQIIYDKQQNFINYYKAQKEENPDLIIDDIVKTYLSNEYSKDIDELN FT AYIEESLNKVTENSGNDVRNFEEFKTGEVFNLYEQESVERWNLAGASDILRVAILKNIG FT GVYLDVDMLPGIHPDLFKDINKPDSVKTAVDWEEMQLEAIMKHKEYIPEYTSKHFDTLD FT EEVQSSFESVLASKSDKSEIFLPLGDIEVSPLEVKIAFAKGSIINQALISAKDSYCSDL FT LIKQIQNRYKILNDTLGPIISQGNDFNTTMNNFGESLGAIANEENISFIAKIGSYLRVG FT FYPEANTTITLSGPTIYAGAYKDLLTFKEMSIDTSILSSELRNFEFPKVNISQATEQEK FT NSLWQFNEERAKIQFEEYKKNYFEGALGEDDNLDFSQNTVTDKEYLLEKISSSTKSSEG FT GYVHYIVQLQGDKISYEAACNLFAKNPYDSILFQRNIEDSEVAYYYNPTDSEIQEIDKY FT RIPDRISDRPKIKLTFIGHGKAEFNTDIFAGLDVDSLSSEIETAIGLAKEDISPKSIEI FT NLLGCNMFSYSVNVEETYPGKLLLRVKDKVSELMPSMSQDSIIVSANQYEVRINSEGRR FT ELLDHSGEWINKEESIIKDISSKEYISFNPKENKIIVKSKNLPELSTLLQEIRNNSNSS FT DIELEEKVMLAECEINVISNIETQVVEERIEEAKSLTSDSINYIKNEFKLIESISEALC FT DLKQQNELEDSHFISFEDISETDEGFSIRFINKETGESIFVETEKTIFSEYANHITEEI FT SKIKGTIFDTVNGKLVKKVNLDTTHEVNTLNAAFFIQSLIEYNSSKESLSNLSVAMKVQ FT VYAQLFSTGLNTITDAAKVVELVSTALDETIDLLPTLSEGLPIIATIIDGVSLGAAIKE FT LSETSDPLLRQEIEAKIGIMAVNLTTATTAIITSSLGIASGFSILLVPLAGISAGIPSL FT VNNELVLRDKATKVVDYFKHVSLVETEGVFTLLDDKVMMQQDDLVISEIDFNNNSIVLG FT KCEIWRMEGGSGHTVTDDIDHFFSAPSITYREPHLSIYDVLEVQKEELDLSKDLMVLPN FT APNRVFAWETGWTPGLRSLENDGTKLLDRIRDNYEGEFYWRYFAFIADALITTLKPRYE FT DTNIRINLDSNTRSFIVPIITTEYIREKLSYSFYGSGGTYALPLSQYNMGINIELSESD FT VWIIDVDNVVRDVTIESDKIKKGDLIEGILSTLSIEENKIILNSHEINFSGEVNGSNGF FT VSLTFSILEGINAIIEVDLLSKSYKLLISGELKILMLNSNHIQQKIDYIGFNSELQKNI FT PYSFVDSEGKENGFINGSTKEGLFVSELPDVVLISKVYMDDSKPSFGYYSNNLKDVKVI FT TKDNVNILTGYYLKDDIKISLSLTLQDEKTIKLNSVHLDESGVAEILKFMNRKGSTNTS FT DSLMSFLESMNIKSIFVNFLQSNIKFILDANFIISGTTSIGQFEFICDENNNIQPYFIK FT FNTLETNYTLYVGNRQNMIVEPNYDLDDSGDISSTVINFSQKYLYGIDSCVNKVVISPN FT IYTDEINITPVYETNNTYPEVIVLDANYINEKINVNINDLSIRYVWSNDGNDFILMSTS FT EENKVSQVKIRFVNVFKDKTLANKLSFNFSDKQDVPVSEIILSFTPSYYEDGLIGYDLG FT LVSLYNEKFYINNFGMMVSGLIYINDSLYYFKPPVNNLITGFVTVGDDKYYFNPINGGA FT ASIGETIIDDKNYYFNQSGVLQTGVFSTEDGFKYFAPANTLDENLEGEAIDFTGKLIID FT ENIYYFEDNYRGAVEWKELDGEMHYFSPETGKAFKGLNQIGDDKYYFNSDGVMQKGFVS FT INDNKHYFDDSGVMKVGYTEIDGKHFYFAENGEMQIGVFNTEDGFKYFAHHNEDLGNEE FT GEEISYSGILNFNNKIYYFDDSFTAVVGWKDLEDGSKYYFDEDTAEAYIGLSLINDGQY FT YFNDDGIMQVGFVTINDKVFYFSDSGIIESGVQNIDDNYFYIDDNGIVQIGVFDTSDGY FT KYFAPANTVNDNIYGQAVEYSGLVRVGEDVYYFGETYTIETGWIYDMENESDKYYFVPE FT TKKACKGINLIDDIKYYFDEKGIMRTGLISFENNNYYFNENGEIQFGYINIEDKMFYFG FT EDGVMQIGVFNTPDGFKYFAHQNTLDENFEGESINYTGWLGLDEKRYYFTDEYIAATGS FT VIIDGEEYYFDPDTAQLVISE" XX SQ Sequence 7104 BP; 2800 A; 729 C; 1226 G; 2349 T; 0 other; 4113999350 CRC32; atgagtttag ttaatagaaa acagttagaa aaaatggcaa atgtaagatt tcgtgttcag 60 gaagatgaat atgtagcaat attagatgca ttagaagaat atcataatat gtcagaaaat 120 actgtagttg aaaagtatct aaaattaaaa gatataaaca gtttaacaga tacttatata 180 gatacatata aaaaatctgg tcgaaataaa gccttaaaaa aatttaaaga gtacttagtt 240 atagagatat tagaattaaa aaatagcaat ttaactccag tcgagaaaaa tttacatttt 300 atatggattg gagggcaaat aaatgatact gctattaatt atataaatca atggaaagat 360 gtaaatagtg actataatgt taatgttttt tatgatagta atgcattttt gataaacaca 420 ttgaaaaaaa ctataataga atcagcatca aatgataccc ttgaatcatt tagagaaaat 480 ttaaatgatc ctgaatttaa ccacactgca tttttcagaa aacgtatgca aataatctat 540 gataaacagc aaaatttcat aaattactat aaagctcaaa aagaagaaaa tcctgacctt 600 ataattgatg atattgtaaa gacatatctt tcaaacgagt attcaaagga tatagatgaa 660 cttaatgctt atattgaaga gtcattaaac aaagtcacag aaaatagtgg aaatgatgtt 720 agaaactttg aagaatttaa aactggagaa gtattcaatt tatatgaaca agagtcagta 780 gaaagatgga atcttgctgg tgcatctgat atattaagag tcgctatatt gaaaaatatt 840 ggtggagtct atctagatgt tgatatgtta ccaggaatac acccagattt atttaaagat 900 ataaataagc ctgattcagt aaagacagct gtagattggg aagagatgca gttagaagcc 960 ataatgaaac ataaagaata tataccagaa tatacttcaa aacattttga tacattggat 1020 gaagaagttc aaagtagctt tgaatctgtt ttagcttcta agtctgataa gtcagaaata 1080 tttttaccac taggagatat agaggtatca cctttagaag taaaaattgc atttgccaaa 1140 ggttctatta taaatcaagc tctaatttct gcaaaagatt catattgtag tgacttacta 1200 ataaaacaaa tccaaaacag atataagata ctgaatgata ctttaggtcc aattattagt 1260 caaggtaatg attttaatac tacaatgaac aattttggtg aaagtttggg agctatagct 1320 aatgaagaga atataagttt tatagcaaaa atcggaagtt atttaagggt tggattttat 1380 cctgaagcta atactacaat tactttaagt ggtcctacaa tatatgcagg agcttataaa 1440 gatttattaa catttaaaga gatgagcata gatacttcta tattgtcatc tgagttaaga 1500 aattttgaat ttcctaaggt taatatatct caagcaacag aacaagagaa aaatagttta 1560 tggcaattta atgaagaaag agctaaaatt caatttgaag aatacaagaa aaattatttt 1620 gaaggtgcac ttggagaaga tgataatctt gatttttctc aaaatacagt aactgacaaa 1680 gaatatcttt tagaaaagat ctcttcatca acgaagagtt cagaaggagg atatgttcat 1740 tatattgttc aattacaagg agataaaatt agctatgaag cagcatgtaa cttatttgca 1800 aaaaatcctt atgacagtat actatttcaa agaaatatag aagattcaga agtagcatat 1860 tactataatc ctacagatag tgaaatacaa gaaattgata agtatagaat tcctgataga 1920 atctctgata gacctaagat taaattaaca ttcattggtc atggcaaagc tgaatttaat 1980 actgatatat ttgcaggtct tgatgtagat tcattatctt cagaaataga aacagcaata 2040 ggtttagcca aagaggatat ttctcctaaa tctatagaaa taaacttact gggatgtaac 2100 atgtttagct attctgtaaa tgtagaagag acttatcctg ggaaattatt acttagagtt 2160 aaagataaag tatcagaatt aatgccatct atgagtcaag actctattat agtaagtgca 2220 aatcaatatg aagttagaat aaatagtgaa ggaagaagag aattattaga ccattctggt 2280 gaatggataa acaaagaaga aagtattata aaggatattt catcaaaaga atatatatca 2340 tttaatccta aagagaataa aattatagta aaatctaaaa atttacctga attatctaca 2400 ttattacaag aaattagaaa taattctaat tcaagtgata ttgaactaga agaaaaagta 2460 atgttagcag aatgtgagat aaatgttatt tcaaatatag agacacaagt ggtagaagaa 2520 agaattgaag aagctaaaag cttaacttct gactctatta attatataaa gaatgaattt 2580 aaactaatag aatctatttc tgaggcacta tgtgacttaa aacaacagaa tgaattagaa 2640 gattctcatt ttatatcttt tgaggacata tcagagactg atgaggggtt tagtataaga 2700 tttattaata aagaaactgg agaatctata tttgtagaaa ctgaaaaaac aatattctct 2760 gaatatgcta atcatataac tgaagagatt tctaagataa aaggtactat atttgatact 2820 gtaaatggta agttagtaaa aaaagtaaat ttagatacta cacacgaagt aaatacttta 2880 aatgctgcat tttttataca atcattaata gaatataata gttctaaaga atctcttagt 2940 aatttaagtg tagcaatgaa agttcaagtt tacgctcaat tatttagtac tggtttaaat 3000 actattacag atgcagccaa agttgttgaa ttagtatcaa ctgcattaga tgaaactata 3060 gacttacttc ctacattatc tgaaggatta cctataattg caactattat agatggtgta 3120 agtttaggtg cagcaatcaa agagctaagt gaaacgagtg acccattatt aagacaagaa 3180 atagaagcta agataggtat aatggcagta aatttaacaa cagctacaac tgcaatcatt 3240 acttcatctt tggggatagc tagtggattt agtatacttt tagttccttt agcaggaatt 3300 tcagcaggta taccaagctt agtaaacaat gaacttgtac ttcgagataa ggcaacaaag 3360 gttgtagatt attttaaaca tgtttcatta gttgaaactg aaggagtatt tactttatta 3420 gatgataaag taatgatgca acaagatgat ttagtgatat cagaaataga ttttaataat 3480 aattcaatag ttttaggtaa atgtgaaatc tggagaatgg aaggtggttc aggtcatact 3540 gtaactgatg atatagatca cttcttttca gcaccatcaa taacatatag agagccacac 3600 ttatctatat atgacgtatt ggaagtacaa aaagaagaac ttgatttgtc aaaagattta 3660 atggtattac ctaatgctcc aaatagagta tttgcttggg aaacaggatg gacaccaggt 3720 ttaagaagct tagaaaatga tggcacaaaa ctgttagacc gtataagaga taactatgaa 3780 ggtgagtttt attggagata ttttgctttt atagctgatg ctttaataac aacattaaaa 3840 ccaagatatg aagatactaa tataagaata aatttagata gtaatactag aagttttata 3900 gttccaataa taactacaga atatataaga gaaaaattat catattcttt ctatggttca 3960 ggaggaactt atgcattgcc tctttctcaa tataatatgg gtataaatat agaattaagt 4020 gaaagtgatg tttggattat agatgttgat aatgttgtga gagatgtaac tatagaatct 4080 gataaaatta aaaaaggtga tttaatagaa ggtattttat ctacactaag tattgaagag 4140 aataaaatta tcttaaatag ccatgagatt aatttttctg gtgaggtaaa tggaagtaat 4200 ggatttgttt ctttaacatt ttcaatttta gaaggaataa atgcaattat agaagttgat 4260 ttattatcta aatcatataa attacttatt tctggcgaat taaaaatatt gatgttaaat 4320 tcaaatcata ttcaacagaa aatagattat ataggattca atagcgaatt acagaaaaat 4380 ataccatata gctttgtaga tagtgaagga aaagagaatg gttttattaa tggttcaaca 4440 aaagaaggtt tatttgtatc agaattacct gatgtagttc ttataagtaa ggtttatatg 4500 gatgatagta agccttcatt tggatattat agtaataatt tgaaagatgt caaagttata 4560 actaaagata atgttaatat attaacaggt tattatctta aggatgatat aaaaatctct 4620 ctttctttga ctctacaaga tgaaaaaact ataaagttaa atagtgtgca tttagatgaa 4680 agtggagtag ctgagatttt gaagttcatg aatagaaaag gtagtacaaa tacttcagat 4740 tctttaatga gctttttaga aagtatgaat ataaaaagta ttttcgttaa tttcttacaa 4800 tctaatatta agtttatatt agatgctaat tttataataa gtggtactac ttctattggc 4860 caatttgagt ttatttgtga tgaaaataat aatatacaac catatttcat taagtttaat 4920 acactagaaa ctaattatac tttatatgta ggaaatagac aaaatatgat agtggaacca 4980 aattatgatt tagatgattc tggagatata tcttcaactg ttatcaattt ctctcaaaag 5040 tatctttatg gaatagacag ttgtgttaat aaagttgtaa tttcaccaaa tatttataca 5100 gatgaaataa atataacgcc tgtatatgaa acaaataata cttatccaga agttattgta 5160 ttagatgcaa attatataaa cgaaaaaata aatgttaata tcaatgatct atctatacga 5220 tatgtatgga gtaatgatgg taatgatttt attcttatgt caactagtga agaaaataag 5280 gtgtcacaag ttaaaataag attcgttaat gtttttaaag ataagacttt ggcaaataag 5340 ctatctttta actttagtga taaacaagat gtacctgtaa gtgaaataat cttatcgttt 5400 acaccttcat attatgagga tggattgatt ggctatgatt tgggtctagt ttctttatat 5460 aatgagaaat tttatattaa taactttgga atgatggtat ctggattaat atatattaat 5520 gattcattat attattttaa accaccagta aataatttga taactggatt tgtgactgta 5580 ggcgatgata aatactactt taatccaatt aatggtggag ctgcttcaat tggagagaca 5640 ataattgatg acaaaaatta ttatttcaac caaagtggag tgttacaaac aggtgtattt 5700 agtacagaag atggatttaa atattttgcc ccagctaata cacttgatga aaacctagaa 5760 ggagaagcaa ttgattttac tggaaaatta attattgacg aaaatattta ttattttgaa 5820 gataattata gaggagctgt agaatggaaa gaattagatg gtgaaatgca ctattttagc 5880 ccagaaacag gtaaagcttt taaaggtcta aatcaaatag gtgatgataa atactatttc 5940 aattctgatg gagttatgca aaaaggattt gttagtataa atgataataa acactatttt 6000 gatgattctg gtgttatgaa agtaggttac actgaaatag atggcaagca tttctacttt 6060 gctgaaaacg gagaaatgca aataggagta tttaatacag aagatggatt taaatatttt 6120 gctcatcata atgaagattt aggaaatgaa gaaggtgaag aaatctcata ttctggtata 6180 ttaaatttca ataataaaat ttactatttt gatgattcat ttacagctgt agttggatgg 6240 aaagatttag aggatggttc aaagtattat tttgatgaag atacagcaga agcatatata 6300 ggtttgtcat taataaatga tggtcaatat tattttaatg atgatggaat tatgcaagtt 6360 ggatttgtca ctataaatga taaagtcttc tacttctctg actctggaat tatagaatct 6420 ggagtacaaa acatagatga caattatttc tatatagatg ataatggtat agttcaaatt 6480 ggtgtatttg atacttcaga tggatataaa tattttgcac ctgctaatac tgtaaatgat 6540 aatatttacg gacaagcagt tgaatatagt ggtttagtta gagttggtga agatgtatat 6600 tattttggag aaacatatac aattgagact ggatggatat atgatatgga aaatgaaagt 6660 gataaatatt atttcgttcc agaaactaaa aaagcatgca aaggtattaa tttaattgat 6720 gatataaaat attattttga tgagaagggc ataatgagaa cgggtcttat atcatttgaa 6780 aataataatt attactttaa tgagaatggt gaaatccaat ttggttatat aaatatagaa 6840 gataagatgt tctattttgg tgaagatggt gtcatgcaga ttggagtatt taatacacca 6900 gatggattta aatactttgc acatcaaaat actttggatg agaattttga gggagaatca 6960 ataaactata ctggttggtt aggtttagat gaaaagagat attattttac agatgaatat 7020 attgcagcaa ctggttcagt tattattgat ggtgaggagt attattttga tcctgataca 7080 gctcaattag tgattagtga atag 7104 // |
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