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EBIDatabasesDatabase BrowsingDbfetch

EBI Dbfetch

ID   CAA17206; SV 1; linear; genomic DNA; STD; PRO; 456 BP.
XX
PA   BX842575.1
XX
DE   Mycobacterium tuberculosis H37Rv PROBABLE CELLULASE CELA2B
DE   (ENDO-1,4-BETA-GLUCANASE) (ENDOGLUCANASE) (CARBOXYMETHYL CELLULASE)
XX
OS   Mycobacterium tuberculosis H37Rv
OC   Bacteria; Actinobacteria; Actinobacteridae; Actinomycetales;
OC   Corynebacterineae; Mycobacteriaceae; Mycobacterium;
OC   Mycobacterium tuberculosis complex.
OX   NCBI_TaxID=83332;
XX
FH   Key             Location/Qualifiers
FH
FT   source          1..456
FT                   /organism="Mycobacterium tuberculosis H37Rv"
FT                   /strain="H37Rv"
FT                   /mol_type="genomic DNA"
FT   CDS             BX842575.1:181812..182267
FT                   /transl_table=11
FT                   /gene="celA2b"
FT                   /locus_tag="Rv1090"
FT                   /product="PROBABLE CELLULASE CELA2B
FT                   (ENDO-1,4-BETA-GLUCANASE) (ENDOGLUCANASE) (CARBOXYMETHYL
FT                   CELLULASE)"
FT                   /function="THE BIOLOGICAL CONVERSION OF CELLULOSE TO
FT                   GLUCOSE GENERALLY REQUIRES THREE TYPES OF HYDROLYTIC
FT                   ENZYMES: (1) ENDOGLUCANASES WHICH CUT INTERNAL
FT                   BETA-1,4-GLUCOSIDIC BONDS; (2) EXOCELLOBIOHYDROLASES THAT
FT                   CUT THE DISSACCHARIDE CELLOBIOSE FROM THE NONREDUCING END
FT                   OF THE CELLULOSE POLYMER CHAIN; (3) BETA-1,4-GLUCOSIDASES
FT                   WHICH HYDROLYZE THE CELLOBIOSE AND OTHER SHORT
FT                   CELLO-OLIGOSACCHARIDES TO GLUCOSE [CATALYTIC
FT                   ACTIVITY:Endohydrolysis of 1,4-beta-D-glucosidic linkages
FT                   in cellulose]."
FT                   /EC_number="3.2.1.4"
FT                   /note="Rv1090, (MTV017.43), len: 151 aa. Probable celA2b,
FT                   second part of cellulase (endoglucanase) (EC 3.2.1.4),
FT                   similar to C-terminus of others e.g. O08468 cellulase CEL2
FT                   from Streptomyces halstedi (377 aa), FASTA scores: opt:
FT                   554, E(): 1.2e-30, (52.0% identity in 152 aa overlap); etc.
FT                   TBparse score is 0.876. Gene appears to have been
FT                   inactivated by frameshift mutations but no errors could be
FT                   found that would account for this."
FT                   /db_xref="GOA:O53438"
FT                   /db_xref="HSSP:1OA4"
FT                   /db_xref="InterPro:IPR013319"
FT                   /db_xref="UniProtKB/TrEMBL:O53438"
FT                   /protein_id="CAA17206.1"
FT                   /translation="MGTNLPTEVGQILSAPTSIDYNYPTTGVWDASYDICLDSTPKTTG
FT                   VNQQEIMIWFNHQGSIQPVGSPVGNTTIEGKNFVVWDGSNGMNNAMAYVATEPIEVWSF
FT                   DVMSFVDHTATMEPITDSWYLTSIRAGLEPWSDGVGLGVDSFSAKVN"
XX
SQ   Sequence 456 BP; 96 A; 140 C; 140 G; 80 T; 0 other; 1140139737 CRC32;
     gtgggcacca acctgcctac tgaggtgggc cagattttgt cggcgccgac cagcatcgac        60
     tacaactacc cgacgaccgg ggtatgggac gcctcctacg acatctgcct ggattccaca       120
     cccaagacga ccggggtcaa ccagcaggag atcatgatct ggttcaacca ccagggctcc       180
     attcagccgg tcggctcccc ggtgggcaac accaccatcg agggcaagaa cttcgtggtg       240
     tgggatggca gcaacggcat gaacaacgcg atggcctatg tcgcgaccga gccgatcgag       300
     gtctggagct tcgacgtgat gagtttcgtc gaccacaccg ccaccatgga gccgatcacc       360
     gactcgtggt acctcacgag catccgggcc ggcttggagc cctggagcga cggtgtgggt       420
     ctgggggtcg attcgttctc ggcgaaagtc aactaa                                 456
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