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EBIDatabasesDatabase BrowsingDbfetch

EBI Dbfetch

ID   CAA17195; SV 1; linear; genomic DNA; STD; PRO; 1167 BP.
XX
PA   BX842575.1
XX
DE   Mycobacterium tuberculosis H37Rv PROBABLE CYSTATHIONINE GAMMA-SYNTHASE METB
DE   (CGS) (O-SUCCINYLHOMOSERINE [THIOL]-LYASE)
XX
OS   Mycobacterium tuberculosis H37Rv
OC   Bacteria; Actinobacteria; Actinobacteridae; Actinomycetales;
OC   Corynebacterineae; Mycobacteriaceae; Mycobacterium;
OC   Mycobacterium tuberculosis complex.
OX   NCBI_TaxID=83332;
XX
FH   Key             Location/Qualifiers
FH
FT   source          1..1167
FT                   /organism="Mycobacterium tuberculosis H37Rv"
FT                   /strain="H37Rv"
FT                   /mol_type="genomic DNA"
FT   CDS             BX842575.1:170280..171446
FT                   /transl_table=11
FT                   /gene="metB"
FT                   /locus_tag="Rv1079"
FT                   /product="PROBABLE CYSTATHIONINE GAMMA-SYNTHASE METB (CGS)
FT                   (O-SUCCINYLHOMOSERINE [THIOL]-LYASE)"
FT                   /function="INVOLVED IN METHIONINE BIOSYNTHESIS: CONVERTS
FT                   O-SUCCINYL-L-HOMOSERINE TO CYSTATHIONINE [CATALYTIC
FT                   ACTIVITY : O-SUCCINYL-L-HOMOSERINE + L-CYSTEINE =
FT                   CYSTATHIONINE + SUCCINATE (CAN ALSO USE HYDROGEN SULFIDE
FT                   AND METHANETHIOL AS SUBSTRATES)]."
FT                   /EC_number="2.5.1.48"
FT                   /note="Rv1079, (MTV017.32), len: 388 aa. Probable metB,
FT                   cystathionine gamma-synthase (EC 4.2.99.9) (see citation
FT                   below). P46807|METB_MYCLE CYSTATHIONINE GAMMA-SYNTHASE from
FT                   Mycobacterium leprae (EC 4.2.1.22) (388 aa), FASTA scores:
FT                   opt: 2220, E(): 0, (87.3% identity in 387 aa overlap). Also
FT                   similar to other Mycobacterium tuberculosis enzymes
FT                   involved in methionine synthesis e.g. Rv0391 and Rv3340.
FT                   Contains PS00868 Cys/Met metabolism enzymes
FT                   pyridoxal-phosphate attachment site. TBparse score is
FT                   0.909. BELONGS TO THE TRANS-SULFURATION ENZYMES FAMILY."
FT                   /db_xref="GOA:P66875"
FT                   /db_xref="HSSP:1GC0"
FT                   /db_xref="InterPro:IPR015422"
FT                   /db_xref="UniProtKB/Swiss-Prot:P66875"
FT                   /func_characterised="identical sequence"
FT                   /protein_id="CAA17195.1"
FT                   /translation="MSEDRTGHQGISGPATRAIHAGYRPDPATGAVNVPIYASSTFAQD
FT                   GVGGLRGGFEYARTGNPTRAALEASLAAVEEGAFARAFSSGMAATDCALRAMLRPGDHV
FT                   VIPDDAYGGTFRLIDKVFTRWDVQYTPVRLADLDAVGAAITPRTRLIWVETPTNPLLSI
FT                   ADITAIAELGTDRSAKVLVDNTFASPALQQPLRLGADVVLHSTTKYIGGHSDVVGGALV
FT                   TNDEELDEEFAFLQNGAGAVPGPFDAYLTMRGLKTLVLRMQRHSENACAVAEFLADHPS
FT                   VSSVLYPGLPSHPGHEIAARQMRGFGGMVSVRMRAGRRAAQDLCAKTRVFILAESLGGV
FT                   ESLIEHPSAMTHASTAGSQLEVPDDLVRLSVGIEDIADLLGDLEQALG"
XX
SQ   Sequence 1167 BP; 195 A; 360 C; 396 G; 216 T; 0 other; 217202732 CRC32;
     atgagcgaag accgcacggg acaccaggga atcagcggac cggccacccg cgccatccac        60
     gctggctacc gcccggatcc ggcgaccggg gcggtgaacg tgccgatcta cgccagcagc       120
     accttcgccc aagacggcgt cggcggtctg cgtggcggtt tcgaatacgc acgcaccggc       180
     aaccccaccc gggccgcatt ggaggcctcg ctggcggcag tcgaggaggg tgctttcgcg       240
     cgggcattca gttccgggat ggccgcgacc gactgcgccc tgcgggcgat gttacggccc       300
     ggagaccacg tcgtcattcc cgatgacgcc tacggcggca cattccggtt gatagacaag       360
     gtgttcaccc ggtgggatgt ccagtacacg ccggtgcggc ttgccgatct ggatgcggtg       420
     ggtgccgcga ttactccgcg cacccggctg atttgggtgg agacgcccac caatccgcta       480
     ctgtcgatcg ccgatatcac ggccattgcc gagctgggca cagacagatc ggcaaaagta       540
     ttggtggaca atacctttgc ctcacccgcg ttgcagcagc cgttgcggct gggcgccgat       600
     gtggtgttgc actcgactac caagtacatc ggcggccatt ccgacgtggt gggaggtgcg       660
     ctggtcacca acgacgaaga gctggacgag gagttcgctt tcttgcagaa cggcgccggc       720
     gcggtgcccg gaccattcga cgcctacctg accatgcgcg gcctgaagac cttggtgctg       780
     cggatgcagc ggcacagtga aaatgcctgt gcggtagcgg aattcctcgc tgatcatccg       840
     tcggtgagtt ctgtgttgta tccgggtttg cccagtcatc ccgggcatga gattgccgcg       900
     cgacagatgc gcggcttcgg cggcatggtt tcggtgcgga tgcgggccgg tcggcgtgcg       960
     gcgcaggacc tgtgtgccaa gacccgcgtc ttcatcctgg ccgagtcgct gggtggggtg      1020
     gagtcgctga tcgaacatcc cagcgccatg acccatgcgt cgacggccgg ttcgcaattg      1080
     gaggtgcccg acgatctggt gcggctttcg gtcggtatcg aagacattgc cgacctgctc      1140
     ggcgatctcg aacaggccct gggttaa                                          1167
//
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