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EBIDatabasesDatabase BrowsingDbfetch

EBI Dbfetch

ID   CAA16251; SV 1; linear; genomic DNA; STD; PRO; 1278 BP.
XX
PA   BX842572.1
XX
DE   Mycobacterium tuberculosis H37Rv PROBABLE SERINE HYDROXYMETHYLTRANSFERASE
DE   GLYA2 (SERINE METHYLASE 2) (SHMT 2)
XX
OS   Mycobacterium tuberculosis H37Rv
OC   Bacteria; Actinobacteria; Actinobacteridae; Actinomycetales;
OC   Corynebacterineae; Mycobacteriaceae; Mycobacterium;
OC   Mycobacterium tuberculosis complex.
OX   NCBI_TaxID=83332;
XX
FH   Key             Location/Qualifiers
FH
FT   source          1..1278
FT                   /organism="Mycobacterium tuberculosis H37Rv"
FT                   /strain="H37Rv"
FT                   /mol_type="genomic DNA"
FT   CDS             complement(BX842572.1:77619..78896)
FT                   /transl_table=11
FT                   /gene="glyA2"
FT                   /locus_tag="Rv0070c"
FT                   /product="PROBABLE SERINE HYDROXYMETHYLTRANSFERASE GLYA2
FT                   (SERINE METHYLASE 2) (SHMT 2)"
FT                   /function="KEY ENZYME IN THE BIOSYNTHESIS OF PURINES,
FT                   LIPIDS, OTHER COMPONENTS. INTERCONVERSION OF SERINE AND
FT                   GLYCINE [CATALYTIC ACTIVITY: 5,10-methylenetetrahydrofolate
FT                   + glycine + H2O = tetrahydrofolate + L-serine]."
FT                   /EC_number="2.1.2.1"
FT                   /note="Rv0070c, (MTV030.13c), len: 425 aa. Probable glyA2,
FT                   serine hydroxymethyltransferase (EC 2.1.2.1), equivalent to
FT                   NP_302318.1|NC_002677 serine hydroxymethyltransferase from
FT                   Mycobacterium leprae (426 aa). Also highly similar to many
FT                   e.g. O86565|GLYA_STRCO SERINE HYDROXYMETHYLTRANSFERASE from
FT                   Streptomyces coelicolor (420 aa);
FT                   AAK60516.1|AF327063_1|AF327063 serine
FT                   hydroxymethyltransferase from Corynebacterium glutamicum
FT                   (434 aa); GLYA_ECOLI|P00477 serine hydroxymethyltransferase
FT                   from Escherichia coli (417 aa), FASTA scores: opt: 1462,
FT                   E(): 0, (54.3% identity in 416 aa overlap); etc. Also
FT                   highly similar to MTV017_46 from Mycobacterium
FT                   tuberculosis. Contains PS00096 Serine
FT                   hydroxymethyltransferase pyridoxal-phosphate attachment
FT                   site. BELONGS TO THE SHMT FAMILY. COFACTOR: PYRIDOXAL
FT                   PHOSPHATE."
FT                   /db_xref="GOA:O53615"
FT                   /db_xref="HSSP:1DFO"
FT                   /db_xref="InterPro:IPR019798"
FT                   /db_xref="UniProtKB/Swiss-Prot:O53615"
FT                   /func_characterised="identical sequence"
FT                   /protein_id="CAA16251.1"
FT                   /translation="MNTLNDSLTAFDPDIAALIDGELRRQESGLEMIASENYAPLAVMQ
FT                   AQGSVLTNKYAEGYPGRRYYGGCEFVDGVEQLAIDRVKALFGAEYANVQPHSGATANAA
FT                   TMHALLNPGDTILGLSLAHGGHLTHGMRINFSGKLYHATAYEVSKEDYLVDMDAVAEAA
FT                   RTHRPKMIIAGWSAYPRQLDFARFRAIADEVDAVLMVDMAHFAGLVAAGVHPSPVPHAH
FT                   VVTSTTHKTLGGPRGGIILCNDPAIAKKINSAVFPGQQGGPLEHVIAAKATAFKMAAQP
FT                   EFAQRQQRCLDGARILAGRLTQPDVAERGIAVLTGGTDVHLVLVDLRDAELDGQQAEDR
FT                   LAAVDITVNRNAVPFDPRPPMITSGLRIGTPALAARGFSHNDFRAVADLIAAALTATND
FT                   DQLGPLRAQVQRLAARYPLYPELHRT"
XX
SQ   Sequence 1278 BP; 235 A; 477 C; 368 G; 198 T; 0 other; 2625291959 CRC32;
     atgaacaccc tcaacgactc cctgaccgcc ttcgacccgg acatcgccgc cctgatcgac        60
     ggcgagctgc gccgtcaaga atccggcttg gagatgatcg cttcggagaa ctatgcaccg       120
     ctggccgtga tgcaggccca aggttcggtc ttgaccaaca agtacgccga aggctacccg       180
     ggccggcgct actacggtgg ctgtgaattc gtcgacggtg tcgagcagtt ggctatcgac       240
     cgcgtcaaag cgctctttgg cgccgaatac gccaacgtgc aaccacattc gggggccacc       300
     gccaacgccg ccaccatgca tgcgctgcta aaccccggcg acaccatcct ggggttgtcg       360
     ctggctcatg gcggtcacct gacccacggg atgcggatca acttttccgg caagctctac       420
     cacgccaccg cctacgaggt gtccaaagag gactacctgg tcgacatgga tgccgtcgcc       480
     gaggcagcgc gcacacaccg gcccaaaatg atcatcgccg gctggtcggc gtacccacgc       540
     cagctggatt tcgcccgctt ccgcgccatc gccgacgaag tcgacgccgt gctcatggtg       600
     gatatggcgc atttcgccgg cctggtcgcc gctggcgtgc accccagccc ggtgccgcac       660
     gcccacgtcg tcacctccac cactcacaag acgctcggcg ggccccgcgg cggcatcatc       720
     ttgtgcaatg acccggccat cgccaagaag atcaattccg cggtcttccc tgggcagcag       780
     ggcgggccgc tcgagcatgt catcgcagcc aaggccaccg cattcaagat ggcagcacaa       840
     cctgaattcg cgcagcgcca acaacgttgc ctcgacggcg cgcgcatcct tgccggccgg       900
     ttgacccagc ccgacgtcgc cgaacgtggc atcgcggtgc taaccggcgg caccgatgtg       960
     cacctcgtcc tagtcgacct gcgcgacgcc gaactcgacg gccagcaagc cgaagaccgg      1020
     ttggccgccg tggacatcac cgtcaaccgc aacgcggtac ccttcgaccc tcgtcccccg      1080
     atgatcacct cgggcctgcg aatcggcacc ccggcgctgg ccgcacgcgg cttctcccac      1140
     aacgacttcc gcgccgtggc agacctcatc gcggcggcac tgacggccac caacgacgac      1200
     cagctgggtc cgctgcgcgc ccaggtccag cggctggccg cacgctatcc gctctacccg      1260
     gaactgcacc ggacatga                                                    1278
//
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