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EBIDatabasesDatabase BrowsingDbfetch

EBI Dbfetch

ID   BAC06195; SV 1; linear; genomic DNA; STD; PRO; 1236 BP.
XX
PA   AB078775.1
XX
DE   Bacillus circulans 1,3-(1,3;1,4)-beta-D-glucan 3(4)-glucanohydrolase
XX
OS   Bacillus circulans
OC   Bacteria; Firmicutes; Bacillales; Bacillaceae; Bacillus.
OX   NCBI_TaxID=1397;
XX
FH   Key             Location/Qualifiers
FH
FT   source          1..1236
FT                   /organism="Bacillus circulans"
FT                   /mol_type="genomic DNA"
FT   CDS             AB078775.1:89..1324
FT                   /codon_start=1
FT                   /transl_table=11
FT                   /gene="bglM"
FT                   /product="1,3-(1,3;1,4)-beta-D-glucan
FT                   3(4)-glucanohydrolase"
FT                   /note="Enzyme BglM (42 kDa) degrades Aspergillus oryzae
FT                   cell walls actively. The central domain of enzyme BglM
FT                   shows a bacterial beta-1,3-glucan-hydrolase motif belonging
FT                   to glycosyl hydrolase family 16. The 141 C-terminal amino
FT                   acid sequence is composed of three reiterated amino acid
FT                   sequences and binds to pachyman, lichenan, and A. oryzae
FT                   cell walls. This domain shows a motif of
FT                   carbohydrate-binding module family 13. By proteolytic
FT                   removal of the C-terminal domain in the IAM1165 culture,
FT                   mature BglM was processed to several 27-kDa fragments
FT                   (BglLs) that hydrolyze a soluble beta-1,3-glucan."
FT                   /db_xref="GOA:Q8KKH3"
FT                   /db_xref="HSSP:1AJO"
FT                   /db_xref="InterPro:IPR013320"
FT                   /db_xref="UniProtKB/TrEMBL:Q8KKH3"
FT                   /protein_id="BAC06195.1"
FT                   /translation="MMLRKGICVVILFSLLVVLLPVNKTNAAPNWNLVWSDEFNGTSLN
FT                   RANWTPEIGTGSGGWGNNELQYYTDRAQNVQVTGGNLVITAQKESYGGMNYTSARIKTQ
FT                   DLKSFTYGKVEARIKLPSGQGLWPAFWMLGSNISSVGWPKSGEIDIMERVNNNPYVNGT
FT                   VHWDAGGHADFGRVSGNLDFSQFHVYSIEWDSKYIRWFVDGQQFNEFYIENGTGNTEEF
FT                   QRPFFILLNLAVGGNWPGSPNNSTPFPSQMLVDYVRVYQDTGASNVISDGIYTIASKAS
FT                   GKVMDVVDVSTARGAKIQQWTNYVANNQRFRVESTGDGYYKLTAVHSGKVLDVPSSSTS
FT                   TGVQLQQWDDNGSNAQRWKIVDVGGGYYKLVSKVSGLAVDVASASTADGAVVQQWTDNG
FT                   TDAQKWLFTKIN"
XX
SQ   Sequence 1236 BP; 338 A; 264 C; 352 G; 282 T; 0 other; 913578036 CRC32;
     atgatgctaa gaaaaggaat atgtgtagtt atactgttca gtcttctggt cgttctattg        60
     cctgtcaaca aaaccaatgc cgcaccgaac tggaatttgg tatggagtga cgagttcaac       120
     ggaacctcgc tcaatagagc caattggaca ccggaaatcg gtaccggcag cggcggatgg       180
     ggaaacaatg agctgcagta ttataccgac cgggcacaga atgtgcaggt tactggcggt       240
     aaccttgtca ttacagcgca gaaggaatct tatggcggga tgaattatac ttctgcccgg       300
     atcaaaaccc aagacttgaa gagctttact tacgggaaag tggaggcgcg gatcaagctg       360
     ccatcgggtc aaggcttgtg gccggcgttt tggatgctcg gaagcaacat cagttcggtt       420
     ggctggccga aaagcggaga aattgacatt atggaacggg taaacaataa cccttacgtg       480
     aatggtaccg tacactggga tgcaggtggg catgccgatt ttggacgagt gtccggcaat       540
     ttggatttct cccaattcca tgtctacagc atagaatggg actctaaata tatccgttgg       600
     ttcgtggacg gccagcagtt caatgagttc tatattgaga atggaaccgg taataccgag       660
     gaattccagc gcccgttctt tatattattg aatctggcgg tcggcggaaa ttggcctgga       720
     agtccgaaca attccacacc gttcccatcg cagatgctgg tggattatgt acgcgtctat       780
     caggacacgg gtgcatcaaa cgtgatcagt gatgggattt acacgatcgc ttccaaggcg       840
     agcggcaaag tgatggatgt tgtggatgtc tccacggcaa ggggagccaa gatacagcaa       900
     tggaccaact acgtcgccaa taatcaaaga ttcagagtag aaagcaccgg agacgggtac       960
     tacaagctca cagccgtaca cagcggaaaa gttctcgacg tgccgagttc atccacatcc      1020
     actggcgttc agctgcagca atgggatgat aatggatcga atgcgcagcg atggaagatc      1080
     gttgatgtgg gcggagggta ttataaactt gtgtccaagg tgagcggact ggcagtcgat      1140
     gtagccagtg cttccacagc agatggggct gttgtccagc agtggacgga caatgggacg      1200
     gatgcacaaa agtggttgtt caccaaaatt aactaa                                1236
//
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