EBI Dbfetch

ID   AAC97119; SV 1; linear; genomic DNA; STD; PRO; 1077 BP.
XX
PA   AF027867.1
XX
DE   Streptomyces achromogenes SacI restriction endonuclease
XX
OS   Streptomyces achromogenes
OC   Bacteria; Actinobacteria; Actinobacteridae; Actinomycetales;
OC   Streptomycineae; Streptomycetaceae; Streptomyces.
OX   NCBI_TaxID=67255;
XX
FH   Key             Location/Qualifiers
FH
FT   source          1..1077
FT                   /organism="Streptomyces achromogenes"
FT                   /mol_type="genomic DNA"
FT   CDS             AF027867.1:1233..2309
FT                   /codon_start=1
FT                   /transl_table=11
FT                   /gene="sacIR"
FT                   /product="SacI restriction endonuclease"
FT                   /function="cleave DNA sequence 5'GAGCTC3' between the 5th
FT                   and 6th bases to generate a 4-base 3' overhang"
FT                   /note="SacI restriction enzyme"
FT                   /db_xref="GOA:O31074"
FT                   /db_xref="InterPro:IPR019066"
FT                   /db_xref="UniProtKB/Swiss-Prot:O31074"
FT                   /func_characterised="identical sequence"
FT                   /protein_id="AAC97119.1"
FT                   /translation="MGITIKKSTAEQVLRKAYEAAASDDVFLEDWIFLATSLREVDAPR
FT                   TYTAALVTALLARACDDRVDPRSIKEKYDDRAFSLRTLCHGVVVPMSVELGFDLGATGR
FT                   EPINNQPFFRYDQYSEIVRVQTKARPYLDRVSSALARVDEEDYSTEESFRALVAVLAVC
FT                   ISVANKKQRVAVGSAIVEASLIAETQSFVVSGHDVPRKLQACVAAGLDMVYSEVVSRRI
FT                   NDPSRDFPGDVQVILDGDPLLTVEVRGKSVSWEGLEQFVSSATYAGFRRVALMVDAASH
FT                   VSLMSADDLTSALERKYECIVKVNESVSSFLRDVFVWSPRDVHSILSAFPEAMYRRMIE
FT                   IEVREPELDRWAEIFPET"
XX
SQ   Sequence 1077 BP; 216 A; 271 C; 337 G; 253 T; 0 other; 3598890992 CRC32;
     atgggaataa caattaaaaa gagcacggcg gagcaggttt tgaggaaggc gtacgaggcg        60
     gccgcttcgg atgatgtatt cctcgaagat tggatatttc tggctacttc gctacgcgag       120
     gtagatgctc caaggactta cacggctgcg cttgtgacag cactgctcgc gcgtgcatgt       180
     gacgacaggg ttgacccacg ctctatcaaa gagaagtacg acgatcgcgc cttctctctg       240
     cgtacccttt gccacggtgt agtggttccg atgtccgtag aacttggatt tgatctgggg       300
     gctacgggcc gcgagccgat caacaatcag ccattcttcc gttacgacca atacagcgag       360
     atcgtcaggg tccagacgaa ggcgcgcccc tatctggacc gagtaagcag tgcccttgct       420
     agggtcgatg aagaagacta ttccactgaa gagtcgttcc gagcgcttgt cgctgtcttg       480
     gccgtgtgta tctctgtggc gaacaaaaag cagcgcgttg ccgtcgggag tgccatcgtc       540
     gaggcgagcc taatcgctga gactcagagc ttcgttgtga gtggccacga cgttccacga       600
     aagctgcagg cttgtgttgc ggctggtctt gacatggtct acagcgaggt cgtctcgcgg       660
     aggatcaatg acccttctcg cgactttcct ggtgacgtcc aagtgatttt ggacggcgat       720
     ccattgctta ccgttgaagt gcgtggaaag agcgtgtcgt gggaggggct tgagcaattc       780
     gtcagcagtg caacgtatgc cggcttccga cgagttgcgc tcatggtcga tgcggcgtcg       840
     cacgtgtcgc ttatgtcggc cgatgatctg accagcgcgc ttgagcggaa gtacgagtgc       900
     atcgtcaagg tgaacgagag cgtgtcgagc ttcctgcgtg acgtattcgt ctggtcgcct       960
     cgcgacgtcc actcgattct ttcggccttt cctgaggcaa tgtatcgccg catgatagag      1020
     atcgaggtgc gggagccgga gttggatcgg tgggctgaga tcttccctga aacgtga         1077
//