EBI Dbfetch
ID GQ258349; SV 1; circular; other DNA; STD; SYN; 8361 BP.
XX
AC GQ258349;
XX
DT 28-JUL-2009 (Rel. 101, Created)
DT 28-JUL-2009 (Rel. 101, Last updated, Version 1)
XX
DE Cloning vector pRama-34, complete sequence.
XX
KW .
XX
OS Cloning vector pRama-34
OC other sequences; artificial sequences; vectors.
XX
RN [1]
RP 1-8361
RA Ganguly S., Liu J., Pillai V.B., Mittler R.S., Amara R.R.;
RT "Adjuvantive effects of anti-4-1BB agonist Ab and 4-1BBL DNA for a HIV-1
RT Gag DNA vaccine: Different effects on cellular and humoral immunity";
RL Unpublished.
XX
RN [2]
RP 1-8361
RA Ganguly S., Amara R.R.;
RT ;
RL Submitted (08-JUN-2009) to the EMBL/GenBank/DDBJ databases.
RL Microbiology and Immunology, Emory University, 954 Gatewood Road NE,
RL Atlanta, GA 30329, USA
XX
FH Key Location/Qualifiers
FH
FT source 1..8361
FT /organism="Cloning vector pRama-34"
FT /mol_type="other DNA"
FT /db_xref="taxon:658872"
FT promoter 1..690
FT /note="CMV immediate early promoter"
FT intron 691..1638
FT /number=A
FT /note="CMV intron A"
FT CDS 1650..3185
FT /codon_start=1
FT /transl_table=11
FT /product="clade B consensus optimized HIV-1 Gag"
FT /protein_id="ACT68286.1"
FT /translation="MGARASVLSGGELDRWEKIRLRPGGKKKYKLKHIVWASRELERFA
FT VNPGLLETSEGCRQILGQLQPSLQTGSEELRSLYNTVATLYCVHQRIEIKDTKEALDKI
FT EEEQNKSKKKAQQAAADTGHSSQVSQNYPIVQNIQGQMVHQAISPRTLNAWVKVVEEKA
FT FSPEVIPMFSALSEGATPQDLNTMLNTVGGHQAAMQMLKETINEEAAEWDRVHPVHAGP
FT IAPGQMREPRGSDIAGTTSTLQEQIGWMTNNPPIPVGEIYKRWIILGLNKIVRMYSPTS
FT ILDIRQGPKEPFRDYVDRFYKTLRAEQASQEVKNWMTETLLVQNANPDCKTILKALGPA
FT ATLEEMMTACQGVGGPGHKARVLAEAMSQVTNTATIMMQRGNFRNQRKMVKSFNSGKEG
FT HTARNCRAPRKKGSWKSGKEGHQMKDCTERQANFLGKIWPSYKGRPGNFLQSRPEPTAP
FT PFLQSRPEPTAPPEESFRSGVETTTPPQKQEPIDKELYPLTSLRSLFGNDPSS"
FT polyA_signal complement(3222..3444)
FT /note="bovine growth hormone polyadenylation signal"
FT terminator complement(3445..3479)
FT /note="Lambda To terminator"
FT promoter 3534..4223
FT /note="CMV immediate early promoter"
FT intron 4224..5171
FT /number=A
FT /note="CMV intron A"
FT CDS 5292..6221
FT /codon_start=1
FT /transl_table=11
FT /product="4-1BBL"
FT /note="co-stimulatory molecule, CD137 ligand"
FT /protein_id="ACT68287.1"
FT /translation="MDQHTLDVEDTADARHPAGTSCPSDAALLRDTGLLADAALLSDTV
FT RPTNAALPTDAAYPAVNVRDREAAWPPALNFCSRHPKLYGLVALVLLLLIAACVPIFTR
FT TEPRPALTITTSPNLGTRENNADQVTPVSHIGCPNTTQQGSPVFAKLLAKNQASLCNTT
FT LNWHSQDGAGSSYLSQGLRYEEDKKELVVDSPGLYYVFLELKLSPTFTNTGHKVQGWVS
FT LVLQAKPQVDDFDNLALTVELFPCSMENKLVDRSWSQLLLLKAGHRLSVGLRAYLHGAQ
FT DAYRDWELSYPNTTSFGLFLVKPDNPWE"
FT polyA_signal 6225..6447
FT /note="bovine growth hormone polyadenylation signal"
FT terminator 6448..6482
FT /note="lambda To terminator"
FT CDS complement(6503..7297)
FT /codon_start=1
FT /transl_table=11
FT /product="kanamycin resistance protein"
FT /protein_id="ACT68288.1"
FT /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
FT VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
FT SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
FT GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
FT LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
XX
SQ Sequence 8361 BP; 1869 A; 2412 C; 2194 G; 1884 T; 2 other;
cgacaatatt ggctattggc cattgcatac gttgtatcta tatcataata tgtacattta 60
tattggctca tgtccaatat gaccgccatg ttgacattga ttattgacta gttattaata 120
gtaatcaatt acgggttcat tagttcatag cccatatatg gagttccgcg ttacataact 180
tacggtaaat ggcccgcctg gctgaccgcc caacgacccc cgcccattga cgtcaataat 240
gacgtatgtt cccatagtaa cgccaatagg gactttccat tgacgtcaat gggtggagta 300
tttacggtaa actgcccact tggcagtaca tcaagtgtat catatgccaa gtccgccccc 360
tattgacgtc aatgacggta aatggcccgc ctggcattat gcccagtaca tgaccttacg 420
ggactttcct acttggcagt acatctacgg tattagtcat cggctattac catggtgatg 480
cggttttggc agtacaccaa tgggcgtgga tagcggtttg actcacgggg atttccaagt 540
ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg ggactttcca 600
aaatgtcgta ataaccccgc cccgttgacg caaatgggcg gtaggcgtgt acggtgggag 660
gtctatataa gcagagctcg tttagtgaac cgtcagatcg cctggagacg ccatccacgc 720
tgttttgacc tccatagaag acaccgggac cgatccagcc tccgcggccg ggaacggtgc 780
attggaacgc ggattccccg tgccaagagt gacgtaagta ccgcctatag actctatagg 840
cacacccctt tggctcttat gcatgctata ctgtttttgg cttggggcct atacaccccc 900
gcttccttat gctataggtg atggtatagc ttagcctata ggtgtgggtt attgaccatt 960
attgaccact cccctattgg tgacgatact ttccattact aatccataac atggctcttt 1020
gccacaacta tctctattgg ctatatgcca atactctgtc cttcagagac tgacacggac 1080
tctgtatttt tacaggatgg ggtcccattt attatttaca aattcacata tacaacaacg 1140
ccgtcccccg tgcccgcagt ttttattaaa catagcgtgg gatctccacg cgaatctcgg 1200
gtaccgtgtt ccggacatgg gytcttctcc ggtagcggcg gagcttccac atccgagccc 1260
tggtcccatg cctccagcgg ctcatggtcg ctcggcagct ccttgctcct aacagtggag 1320
gccagactta ggcacagcac aatgcccacc accaccagtg tgccgcacaa ggccgtggcg 1380
gtagggtatg tgtctgaaaa tgagctcgga gattgggctc gcaccgctga cgcagatgga 1440
agacttaagg cagcggcaga agaagatgca ggcagctgag ttgttgtatt ctgataagag 1500
tcagaggtaa ctcccgttgc ggtgctgtta acggtggagg gcagtgtagt ctgagcagta 1560
ctcgttgctg ccgcgcgcgc caccagacat aatagctgac agactaacag actgttcctt 1620
tccatgggtc ttttctgcag tcaccatcga tgggcgcccg cgcctccgtg ctgtccggcg 1680
gcgagctgga ccgctgggag aagatccgcc tgcgccccgg cggcaagaag aagtacaagc 1740
tgaagcacat cgtgtgggcc tcccgcgagc tggagcgctt cgccgtgaac cccggcctgc 1800
tggagacctc cgagggctgc cgccagatcc tgggccagct gcagccctcc ctgcagaccg 1860
gctccgagga gctgcgctcc ctgtacaaca ccgtggccac cctgtactgc gtgcaccagc 1920
gcatcgagat caaggacacc aaggaggccc tggacaagat cgaggaggag cagaacaagt 1980
ccaagaagaa ggcccagcag gccgccgccg acaccggcca ctcctcccag gtgtcccaga 2040
actaccccat cgtgcagaac atccagggcc agatggtgca ccaggccatc tccccccgca 2100
ccctgaacgc ctgggtgaag gtggtggagg agaaggcctt ctcccccgag gtgatcccca 2160
tgttctccgc cctgtccgag ggcgccaccc cccaggacct gaacaccatg ctgaacaccg 2220
tgggcggcca ccaggccgcc atgcagatgc tgaaggagac catcaacgag gaggccgccg 2280
agtgggaccg cgtgcacccc gtgcacgccg gccccatcgc ccccggccag atgcgcgagc 2340
cccgcggctc cgacatcgcc ggcaccacct ccaccctgca ggagcagatc ggctggatga 2400
ccaacaaccc ccccatcccc gtgggcgaga tctacaagcg ctggatcatc ctgggcctga 2460
acaagatcgt gcgcatgtac tcccccacct ccatcctgga catccgccaa ggccccaagg 2520
agcccttccg cgactacgtg gaccgcttct acaagaccct gcgcgccgag caggcctccc 2580
aggaggtgaa gaactggatg accgagaccc tgctggtgca gaacgccaac cccgactgca 2640
agaccatcct gaaggccctg ggccccgccg ccaccctgga ggagatgatg accgcctgcc 2700
agggcgtggg cggccccggc cacaaggccc gcgtgctggc cgaggccatg tcccaggtga 2760
ccaacaccgc caccatcatg atgcagcgcg gcaacttccg caaccagcgc aagatggtga 2820
agtccttcaa ctccggcaag gagggccaca ccgcccgcaa ctgccgcgcc ccccgcaaga 2880
agggctcctg gaagtccggc aaggagggcc accagatgaa ggactgcacc gagagacagg 2940
ctaatttttt agggaagatc tggccttcct acaagggaag gccagggaat tttcttcaga 3000
gcagaccaga gccaacagcc ccaccatttc ttcagagcag accagagcca acagccccac 3060
cagaagagag cttcaggtct ggggtagaga caacaactcc ccctcagaag caggagccga 3120
tagacaagga actgtatcct ttaacttccc tcagatcact ctttggcaac gacccctcgt 3180
cttaagctag ccccgggtga taaacggacc gcgcaatccc taggctgtgc cttctagttg 3240
ccagccatct gttgtttgcc cctcccccgt gccttccttg accctggaag gtgccactcc 3300
cactgtcctt tcctaataaa atgaggaaat tgcatcgcat tgtctgagta ggtgtcattc 3360
tattctgggg ggtggggtgg ggcaggacag caagggggag gattgggaag acaatagcag 3420
gcatgctggg gatgcggtgg gctctatata aaaaacgccc ggcggcaacc gagcgttctg 3480
agcggccgct ttacggttcc tggccttttg ctggcctttt gctcacatgt tgtcgacaat 3540
attggctatt ggccattgca tacgttgtat ctatatcata atatgtacat ttatattggc 3600
tcatgtccaa tatgaccgcc atgttgacat tgattattga ctagttatta atagtaatca 3660
attacgggtt cattagttca tagcccatat atggagttcc gcgttacata acttacggta 3720
aatggcccgc ctggctgacc gcccaacgac ccccgcccat tgacgtcaat aatgacgtat 3780
gttcccatag taacgccaat agggactttc cattgacgtc aatgggtgga gtatttacgg 3840
taaactgccc acttggcagt acatcaagtg tatcatatgc caagtccgcc ccctattgac 3900
gtcaatgacg gtaaatggcc cgcctggcat tatgcccagt acatgacctt acgggacttt 3960
cctacttggc agtacatcta cggtattagt catcggctat taccatggtg atgcggtttt 4020
ggcagtacac caatgggcgt ggatagcggt ttgactcacg gggatttcca agtctccacc 4080
ccattgacgt caatgggagt ttgttttggc accaaaatca acgggacttt ccaaaatgtc 4140
gtaataaccc cgccccgttg acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata 4200
taagcagagc tcgtttagtg aaccgtcaga tcgcctggag acgccatcca cgctgttttg 4260
acctccatag aagacaccgg gaccgatcca gcctccgcgg ccgggaacgg tgcattggaa 4320
cgcggattcc ccgtgccaag agtgacgtaa gtaccgccta tagactctat aggcacaccc 4380
ctttggctct tatgcatgct atactgtttt tggcttgggg cctatacacc cccgcttcct 4440
tatgctatag gtgatggtat agcttagcct ataggtgtgg gttattgacc attattgacc 4500
actcccctat tggtgacgat actttccatt actaatccat aacatggctc tttgccacaa 4560
ctatctctat tggctatatg ccaatactct gtccttcaga gactgacacg gactctgtat 4620
ttttacagga tggggtccca tttattattt acaaattcac atatacaaca acgccgtccc 4680
ccgtgcccgc agtttttatt aaacatagcg tgggatctcc acgcgaatct cgggtaccgt 4740
gttccggaca tgggytcttc tccggtagcg gcggagcttc cacatccgag ccctggtccc 4800
atgcctccag cggctcatgg tcgctcggca gctccttgct cctaacagtg gaggccagac 4860
ttaggcacag cacaatgccc accaccacca gtgtgccgca caaggccgtg gcggtagggt 4920
atgtgtctga aaatgagctc ggagattggg ctcgcaccgc tgacgcagat ggaagactta 4980
aggcagcggc agaagaagat gcaggcagct gagttgttgt attctgataa gagtcagagg 5040
taactcccgt tgcggtgctg ttaacggtgg agggcagtgt agtctgagca gtactcgttg 5100
ctgccgcgcg cgccaccaga cataatagct gacagactaa cagactgttc ctttccatgg 5160
gtcttttctg cagtcaccat cgatgcttgc aatcatggat gcaatgaaga gagggctctg 5220
ctgtgtgctg ctgctgtgtg gagaattctt cgtttctgct gctgtgtgga gaattcttcg 5280
tttcggctag catggaccag cacacacttg atgtggagga taccgcggat gccagacatc 5340
cagcaggtac ttcgtgcccc tcggatgcgg cgctcctcag agataccggg ctcctcgcgg 5400
acgctgcgct cctctcagat actgtgcgcc ccacaaatgc cgcgctcccc acggatgctg 5460
cctaccctgc ggttaatgtt cgggatcgcg aggccgcgtg gccgcctgca ctgaacttct 5520
gttcccgcca cccaaagctc tatggcctag tcgctttggt tttgctgctt ctgatcgccg 5580
cctgtgttcc tatcttcacc cgcaccgagc ctcggccagc gctcacaatc accacctcgc 5640
ccaacctggg tacccgagag aataatgcag accaggtcac ccctgtttcc cacattggct 5700
gccccaacac tacacaacag ggctctcctg tgttcgccaa gctactggct aaaaaccaag 5760
catcgttgtg caatacaact ctgaactggc acagccaaga tggagctggg agctcatacc 5820
tatctcaagg tctgaggtac gaagaagaca aaaaggagtt ggtggtagac agtcccgggc 5880
tctactacgt atttttggaa ctgaagctca gtccaacatt cacaaacaca ggccacaagg 5940
tgcagggctg ggtctctctt gttttgcaag caaagcctca ggtagatgac tttgacaact 6000
tggccctgac agtggaactg ttcccttgct ccatggagaa caagttagtg gaccgttcct 6060
ggagtcaact gttgctcctg aaggctggcc accgcctcag tgtgggtctg agggcttatc 6120
tgcatggagc ccaggatgca tacagagact gggagctgtc ttatcccaac accaccagct 6180
ttggactctt tcttgtgaaa cccgacaacc catgggaatg acctaggctg tgccttctag 6240
ttgccagcca tctgttgttt gcccctcccc cgtgccttcc ttgaccctgg aaggtgccac 6300
tcccactgtc ctttcctaat aaaatgagga aattgcatcg cattgtctga gtaggtgtca 6360
ttctattctg gggggtgggg tggggcagga cagcaagggg gaggattggg aagacaatag 6420
caggcatgct ggggatgcgg tgggctctat ataaaaaacg cccggcggca accgagcgtt 6480
ctgaacgcta gagtcgatcg attcagaaga actcgtcaag aaggcgatag aaggcgatgc 6540
gctgcgaatc gggagcggcg ataccgtaaa gcacgaggaa gcggtcagcc cattcgccgc 6600
caagctcttc agcaatatca cgggtagcca acgctatgtc ctgatagcgg tctgccacac 6660
ccagccggcc acagtcgatg aatccagaaa agcggccatt ttccaccatg atattcggca 6720
agcaggcatc gccatgggtc acgacgagat cctcgccgtc gggcatgctc gccttgagcc 6780
tggcgaacag ttcggctggc gcgagcccct gatgctcttc gtccagatca tcctgatcga 6840
caagaccggc ttccatccga gtacgtgctc gctcgatgcg atgtttcgct tggtggtcga 6900
atgggcaggt agccggatca agcgtatgca gccgccgcat tgcatcagcc atgatggata 6960
ctttctcggc aggagcaagg tgagatgaca ggagatcctg ccccggcact tcgcccaata 7020
gcagccagtc ccttcccgct tcagtgacaa cgtcgagcac agctgcgcaa ggaacgcccg 7080
tcgtggccag ccacgatagc cgcgctgcct cgtcttgcag ttcattcagg gcaccggaca 7140
ggtcggtctt gacaaaaaga accgggcgcc cctgcgctga cagccggaac acggcggcat 7200
cagagcagcc gattgtctgt tgtgcccagt catagccgaa tagcctctcc acccaagcgg 7260
ccggagaacc tgcgtgcaat ccatcttgtt caatcatgcg aaacgatcct catcctgtct 7320
cttgatcaga tcttgatccc ctgcgccatc agatccttgg cggcaagaaa gccatccagt 7380
ttactttgca gggcttccca accttaccag agggcgcccc agctggcaat tccggttcgc 7440
ttgctgtcca taaaaccgcc cagtctagct atcgccatgt aagcccactg caagctacct 7500
gctttctctt tgcgcttgcg ttttcccttg tccagatagc ccagtagctg acattcatcc 7560
ggggtcagca ccgtttctgc ggactggctt tctacgtgaa aaggatctag gtgaagatcc 7620
tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag 7680
accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct 7740
gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac 7800
caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgttcttc 7860
tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg 7920
ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt 7980
tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt 8040
gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc 8100
tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca 8160
gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata 8220
gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg 8280
ggcggagcct atggaaaaac gccagcaacg cggccctttt acggttcctg gccttttgct 8340
ggccttttgc tcacatgttg t 8361
//
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