Group Accession SAMEG110753

Submission Title:GSE39179: TAL1 complex in human T-cell acute lymphoblastic leukemia (RPMI-8402)
Submission Identifier:GEN-SRP014150
Submission Description:Summary: The oncogenic transcription factor TAL1/SCL is aberrantly overexpressed in over 40% of cases of T-cell acute lymphoblastic leukemia (T-ALL), emphasizing the importance of the TAL1-regulated transcriptional program in the molecular pathogenesis of T-ALL. Here we identify regions occupied by TAL1 and its regulatory partners HEB, E2A, and GATA3 in a T-ALL cell line (RPMI-8402). Overall Design: Human T-ALL cells were cross-linked with formaldehyde for 20 min. DNA was enriched by chromatin immunoprecipitation (ChIP) and analyzed by Solexa sequencing. A sample of whole cell extract (WCE) was sequenced and used as the background to determine enrichment. ChIP was performed using an antibody against total TAL1 (Santa Cruz SC-12984), TCF12/HEB (Santa Cruz SC-357),TCF3/E2A (Santa Cruz SC-349X), LMO1 (Santa Cruz SC-10494), LMO2 (R&D Systems AF2726), GATA3 (Santa Cruz SC-22206) and RUNX1 (Santa Cruz SC-8563). This submission represents the ChIP-seq portion of this dataset.
Submission Release Date:2013-10-07
Submission Update Date:2014-03-02
Submission Reference Layer:false
Databases:ENA SRA Link

URI: ;

ID: SRP014150 ;

Name: GEO; Address: ; Role: Submitter; Email: ; URI:
Group Name : Other Group
Samples in group:10
Common attributes:
cell typestrainOrganism

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