Millard2020 - Kinetic model of Glucose and Acetate metabolisms in E. coli

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Calibrated kinetic model of glucose and acetate metabolisms of Escherichia coli, as detailed in Millard et al., 2020 (DOI: 10.1101/2020.08.18.255356).
Related Publication
  • Control and regulation of acetate overflow in Escherichia coli
  • Millard, Enjalbert, Uttenweiler-Joseph, Portais, LĂ©tisse
  • BioRxiv preprint , 8/ 2020 , DOI: 10.1101/2020.08.18.255356
  • TBI, UniversitĂ© de Toulouse, CNRS, INRAE, INSA, Toulouse, France
  • Overflow metabolism refers to the production of seemingly wasteful by-products by cells during growth on glucose even when oxygen is abundant. Two theories have been proposed to explain acetate overflow in Escherichia coli - global control of the central metabolism and local control of the acetate pathway - but neither accounts for all observations. Here, we develop a kinetic model of E. coli metabolism that quantitatively accounts for observed behaviors and successfully predicts the response of E. coli to new perturbations. We reconcile these theories and clarify the origin, control and regulation of the acetate flux. We also find that, in turns, acetate regulates glucose metabolism by coordinating the expression of glycolytic and TCA genes. Acetate should not be considered a wasteful end-product since it is also a co-substrate and a global regulator of glucose metabolism in E. coli. This has broad implications for our understanding of overflow metabolism.

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Mathematical Modelling Ontology MAMO_0000040

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Millard2020_Ecoli_glc_ace_kinetic_model.xml SBML L3V1 representation of the calibrated kinetic model of glucose and acetate metabolisms of E. coli - Millard 2020 61.72 KB Preview | Download

  • Model originally submitted by : Millard
  • Submitted: 19-Aug-2020 09:03:16
  • Last Modified: 19-Aug-2020 09:03:16
  • Version: 4 public model Download this version
    • Submitted on: 19-Aug-2020 09:03:16
    • Submitted by: Millard
    • With comment: Edited model metadata online.
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