Aubry1995 - Nine-compartment model of fluid-phase endocytosis kinetics in Dictyostelium discoideum
Model Identifier
BIOMD0000000987
Short description
Fluid-phase endoeytosis (pinocytosis) kinetics were studied in Dictyostelium discoideum amoebae from the axenic strain Ax-2 that exhibits high rates of fluid-phase endoeytosis when cultured in liquid nutrient media. Fluorescein-labelled dextran (FITC-dextran) was used as a marker in continuous uptake- and in pulse-chase exocytosis experiments. In the latter case, efflux of the marker was monitored on cells loaded for short periods of time and resuspended in marker-free medium. A multicompartmental model was developed which describes satisfactorily fluid-phase endocytosis kinetics. In particular, it accounts correctly for the extended latency period before exocytosis in pulse-chase experiments and it suggests the existence of some sorts of maturation stages in the pathway.
Format
SBML
(L2V4)
Related Publication
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Modelling of fluid-phase endocytosis kinetics in the amoebae of the cellular slime mouldDictyostelium discoideum. A multicompartmental approach
- Laurence Aubry, Gérard Klein, Jean-Louis Martiel, Michel Satre
- Acta Biotheoretica , 12/ 1995 , Volume 43 , Issue 4 , pages: 319--333 , DOI: 10.1007/BF00713556
- Laboratoire de Biologie Cellulaire, URA 1130 CNRS, CEA, Département de Biologie Moléculaire et Structurale, Centre d'Etudes Nucléaires, 17, rue des Martyrs, 38054, Grenoble Cedex 9, France
- Fluid-phase endocytosis (pinocytosis) kinetics were studied inDictyostelium discoideum amoebae from the axenic strain Ax-2 that exhibits high rates of fluid-phase endocytosis when cultured in liquid nutrient media. Fluorescein-labelled dextran (FITC-dextran) was used as a marker in continuous uptake- and in pulse-chase exocytosis experiments. In the latter case, efflux of the marker was monitored on cells loaded for short periods of time and resuspended in marker-free medium. A multicompartmental model was developed which describes satisfactorily fluid-phase endocytosis kinetics. In particular, it accounts correctly for the extended latency period before exocytosis in pulse-chase experiments and it suggests the existence of some sorts of maturation stages in the pathway.
Contributors
Kausthubh Ramachandran
Metadata information
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Curation status
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Modelling approach(es)
Tags
| Name | Description | Size | Actions |
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Model files |
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| Aubry1995.xml | SBML L2V4 file of nine-compartmental model of fluid-phase endocytosis kinetics in Dictystelium discoideum | 53.11 KB | Preview | Download |
Additional files |
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| Aubry1995.sedml | SED-ML file of nine-compartmental model of fluid-phase endocytosis kinetics in Dictystelium discoideum | 1.82 KB | Preview | Download |
| Aubry1995.cps | COPASI 4.29 (Build 228) file of nine-compartmental model of fluid-phase endocytosis kinetics in Dictystelium discoideum | 81.95 KB | Preview | Download |
| Aubry1995.omex | COMBINE archive of nine-compartmental model of fluid-phase endocytosis kinetics in Dictystelium discoideum | 11.92 KB | Preview | Download |
- Model originally submitted by : Kausthubh Ramachandran
- Submitted: Feb 10, 2021 9:54:23 AM
- Last Modified: Feb 10, 2021 9:54:23 AM
Revisions
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Version: 2
- Submitted on: Feb 10, 2021 9:54:23 AM
- Submitted by: Kausthubh Ramachandran
- With comment: Automatically added model identifier BIOMD0000000987
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Curator's comment:
(added: 10 Feb 2021, 09:52:17, updated: 10 Feb 2021, 09:52:17)
(added: 10 Feb 2021, 09:52:17, updated: 10 Feb 2021, 09:52:17)
Fig 5 A from the manuscript is reproduced here with parameters as given in the corresponding figure legend. The y-axis units are units of marker fluid per cell.