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BIOMD0000000571 - Nishio2008 - Design of the phosphotransferase system for enhanced glucose uptake in E. coli.

 

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Reference Publication
Publication ID: 18197177
Nishio Y, Usuda Y, Matsui K, Kurata H.
Computer-aided rational design of the phosphotransferase system for enhanced glucose uptake in Escherichia coli.
Mol. Syst. Biol. 2008; 4: 160
Fermentation and Biotechnology Laboratories, Ajinomoto Co. Inc., Kawasaki, Japan. yousuke_nishio@ajinomoto.com  [more]
Model
Original Model: BIOMD0000000571.origin
Submitter: Kieran Smallbone
Submission ID: MODEL1501300000
Submission Date: 30 Jan 2015 10:13:42 UTC
Last Modification Date: 27 Mar 2015 16:08:06 UTC
Creation Date: 01 Jan 2015 12:00:00 UTC
Encoders:  Kieran Smallbone
   Audald Lloret i Villas
   Pierre Millard
set #1
bqmodel:isDerivedFrom BioModels Database Chassagnole2002_Carbon_Metabolism
set #2
bqbiol:isVersionOf Gene Ontology protein-N(PI)-phosphohistidine-sugar phosphotransferase activity
set #3
bqbiol:hasTaxon Taxonomy Escherichia coli (strain K12)
Notes
Nishio2008 - Design of the phosphotransferase system for enhanced glucose uptake in E. coli.

This model is described in the article:

Nishio Y, Usuda Y, Matsui K, Kurata H.
Mol. Syst. Biol. 2008; 4: 160

Abstract:

The phosphotransferase system (PTS) is the sugar transportation machinery that is widely distributed in prokaryotes and is critical for enhanced production of useful metabolites. To increase the glucose uptake rate, we propose a rational strategy for designing the molecular architecture of the Escherichia coli glucose PTS by using a computer-aided design (CAD) system and verified the simulated results with biological experiments. CAD supports construction of a biochemical map, mathematical modeling, simulation, and system analysis. Assuming that the PTS aims at controlling the glucose uptake rate, the PTS was decomposed into hierarchical modules, functional and flux modules, and the effect of changes in gene expression on the glucose uptake rate was simulated to make a rational strategy of how the gene regulatory network is engineered. Such design and analysis predicted that the mlc knockout mutant with ptsI gene overexpression would greatly increase the specific glucose uptake rate. By using biological experiments, we validated the prediction and the presented strategy, thereby enhancing the specific glucose uptake rate.

To the extent possible under law, all copyright and related or neighbouring rights to this encoded model have been dedicated to the public domain worldwide. Please refer to CC0 Public Domain Dedication for more information.

Model
Publication ID: 18197177 Submission Date: 30 Jan 2015 10:13:42 UTC Last Modification Date: 27 Mar 2015 16:08:06 UTC Creation Date: 01 Jan 2015 12:00:00 UTC
Mathematical expressions
Reactions
binding_CRP_cAMP binding_CRP_cAMP_CRPsite_cyaA binding_CRP_cAMP_CRPsiteI_crp binding_CRP_cAMP_CRPsiteII_crp
binding_CRP_cAMP_CRPsite_ptsGp1 binding_CRP_cAMP_CRPsite_ptsGp2 binding_CRP_cAMP_CRPsite_ptsHp0 binding_CRP_cAMP_CRPsite_ptsHp1
binding_CRP_cAMP_CRPsite_ptsIp0 binding_CRP_cAMP_CRPsite_ptsIp1 binding_CRP_cAMP_CRPsite_mlcp1 binding_CRP_cAMP_CRPsite_mlcp2
binding_CRP_cAMP_CRPsite_genome binding_Mlc_Mlcsite_ptsGp1 binding_Mlc_Mlcsite_ptsGp2 binding_Mlc_Mlcsite_ptsHp0
binding_Mlc_Mlcsite_ptsIp0 binding_Mlc_Mlcsite_mlcp1 binding_Mlc_Mlcsite_mlcp2 binding_IICB_Mlc
binding_IIA_P_CYA transcription_CRP_cAMP_CRPsite_cyaA_cyaA transcription_cyaA_basal transcription_CRP_cAMP_CRPsiteI_crp_CRP_cAMP_CRPsiteII_crp_crp
transcription_crp_basal transcription_CRP_cAMP_CRPsite_ptsGp1_Mlc_Mlcsite_ptsGp1_ptsGp1 transcription_CRP_cAMP_CRPsite_ptsGp2_Mlc_Mlcsite_ptsGp2_ptsGp2 transcription_CRP_cAMP_CRPsite_ptsHp0_Mlc_Mlcsite_ptsHp0_ptsHp0
transcription_CRP_cAMP_CRPsite_ptsHp1_ptsHp1 transcription_CRP_cAMP_CRPsite_ptsIp0_Mlc_Mlcsite_ptsIp0_ptsIp0 transcription_CRP_cAMP_CRPsite_ptsIp1_ptsIp1 transcription_crr
transcription_CRP_cAMP_CRPsite_mlcp1_Mlc_Mlcsite_mlcp1_mlcp1 transcription_CRP_cAMP_CRPsite_mlcp2_Mlc_Mlcsite_mlcp2_mlcp2 decomposition_mRNA_cyaA decomposition_mRNA_crp
decomposition_mRNA_ptsG decomposition_mRNA_ptsH decomposition_mRNA_ptsI decomposition_mRNA_crr
decomposition_mRNA_mlc translation_mRNA_cyaA translation_mRNA_crp translation_mRNA_ptsG
translation_mRNA_ptsH translation_mRNA_ptsI translation_mRNA_crr translation_mlc
decomposition_CYA decomposition_CRP decomposition_Mlc decomposition_cAMP
decomposition_CRP_cAMP decomposition_CRP_cAMP_CRPsite_cyaA decomposition_CRP_cAMP_CRPsiteI_crp decomposition_CRP_cAMP_CRPsiteII_crp
decomposition_CRP_cAMP_CRPsite_ptsGp1 decomposition_CRP_cAMP_CRPsite_ptsGp2 decomposition_CRP_cAMP_CRPsite_ptsHp0 decomposition_CRP_cAMP_CRPsite_ptsHp1
decomposition_CRP_cAMP_CRPsite_ptsIp0 decomposition_CRP_cAMP_CRPsite_ptsIp1 decomposition_CRP_cAMP_CRPsite_mlcp1 decomposition_CRP_cAMP_CRPsite_mlcp2
decomposition_CRP_cAMP_CRPsite_genome decomposition_Mlc_Mlcsite_ptsGp1 decomposition_Mlc_Mlcsite_ptsGp2 decomposition_Mlc_Mlcsite_ptsHp0
decomposition_Mlc_Mlcsite_ptsIp0 decomposition_Mlc_Mlcsite_mlcp1 decomposition_Mlc_Mlcsite_mlcp2 decomposition_IICB_Mlc
decomposition_EI_P decomposition_EI decomposition_HPr_P decomposition_HPr
decomposition_IIA_P decomposition_IIA decomposition_IICB_P decomposition_IICB
PTS2for PTS2rev PTS3for PTS3rev
PTS4for PTS4rev reaction_CYA_ATP reaction_IIA_P_CYA_ATP
reaction_EI_PEP reaction_EIP_Pyr reaction_IICB_P_Glucose reaction_IICB_Glc6P
Rules
Assignment Rule (variable: TCRPsite_cyaA) Assignment Rule (variable: TCRPsiteI_crp) Assignment Rule (variable: TCRPsiteII_crp) Assignment Rule (variable: TCRPsite_ptsGp1)
Assignment Rule (variable: TMlcsite_ptsGp1) Assignment Rule (variable: TCRPsite_ptsGp2) Assignment Rule (variable: TMlcsite_ptsGp2) Assignment Rule (variable: TCRPsite_ptsHp0)
Assignment Rule (variable: TMlcsite_ptsHp0) Assignment Rule (variable: TCRPsite_ptsHp1) Assignment Rule (variable: TCRPsite_ptsIp0) Assignment Rule (variable: TMlcsite_ptsIp0)
Assignment Rule (variable: TCRPsite_ptsIp1) Assignment Rule (variable: TCRPsite_mlcp1) Assignment Rule (variable: TMlcsite_mlcp1) Assignment Rule (variable: TCRPsite_mlcp2)
Assignment Rule (variable: TMlcsite_mlcp2)      
Events
       
Physical entities
Compartments Species
cyt CRP CRPsiteI_crp CRPsiteII_crp
CRPsite_cyaA CRPsite_genome CRPsite_ptsGp1
CRPsite_ptsGp2 CRPsite_ptsHp0 CRPsite_ptsHp1
CRPsite_ptsIp0 CRPsite_ptsIp1 CRPsite_mlcp1
CRPsite_mlcp2 Mlc Mlcsite_mlcp1
Mlcsite_mlcp2 Mlcsite_ptsGp1 Mlcsite_ptsGp2
Mlcsite_ptsHp0 Mlcsite_ptsIp0 CRP_cAMP
CRP_cAMP_CRPsiteI_crp CRP_cAMP_CRPsiteII_crp CRP_cAMP_CRPsite_cyaA
CRP_cAMP_CRPsite_genome CRP_cAMP_CRPsite_ptsGp1 CRP_cAMP_CRPsite_ptsGp2
CRP_cAMP_CRPsite_ptsHp0 CRP_cAMP_CRPsite_ptsHp1 CRP_cAMP_CRPsite_ptsIp0
CRP_cAMP_CRPsite_ptsIp1 CRP_cAMP_CRPsite_mlcp1 CRP_cAMP_CRPsite_mlcp2
Mlc_Mlcsite_ptsGp1 Mlc_Mlcsite_ptsGp2 Mlc_Mlcsite_ptsIp0
Mlc_Mlcsite_ptsHp0 Mlc_Mlcsite_mlcp1 Mlc_Mlcsite_mlcp2
IICB IICB_Mlc CYA
IIA_P IIA_P_CYA mRNA_cyaA
mRNA_crp mRNA_ptsG mRNA_ptsH
mRNA_ptsI mRNA_crr mRNA_mlc
IICB_P IIA HPr_P
HPr EI_P EI
cAMP cyaA cyaA_basal
crp crp_basal ptsGp1
ptsGp2 ptsHp0 ptsHp1
ptsIp0 ptsIp1 crr
mlcp1 mlcp2 Pyr
PEP Glc6P Glucose
ATP    
Global parameters
fast TCRPsite_cyaA TCRPsiteI_crp TCRPsiteII_crp
TCRPsite_ptsGp1 TMlcsite_ptsGp1 TCRPsite_ptsGp2 TMlcsite_ptsGp2
TCRPsite_ptsHp0 TMlcsite_ptsHp0 TCRPsite_ptsHp1 TCRPsite_ptsIp0
TMlcsite_ptsIp0 TCRPsite_ptsIp1 TCRPsite_mlcp1 TMlcsite_mlcp1
TCRPsite_mlcp2 TMlcsite_mlcp2    
Reactions (92)
 
 binding_CRP_cAMP [CRP] + [cAMP] ↔ [CRP_cAMP];   {CRP} , {cAMP} , {CRP_cAMP}
 
 binding_CRP_cAMP_CRPsite_cyaA [CRP_cAMP] + [CRPsite_cyaA] ↔ [CRP_cAMP_CRPsite_cyaA];   {CRP_cAMP} , {CRPsite_cyaA} , {CRP_cAMP_CRPsite_cyaA}
 
 binding_CRP_cAMP_CRPsiteI_crp [CRP_cAMP] + [CRPsiteI_crp] ↔ [CRP_cAMP_CRPsiteI_crp];   {CRP_cAMP} , {CRPsiteI_crp} , {CRP_cAMP_CRPsiteI_crp}
 
 binding_CRP_cAMP_CRPsiteII_crp [CRP_cAMP] + [CRPsiteII_crp] ↔ [CRP_cAMP_CRPsiteII_crp];   {CRP_cAMP} , {CRPsiteII_crp} , {CRP_cAMP_CRPsiteII_crp}
 
 binding_CRP_cAMP_CRPsite_ptsGp1 [CRP_cAMP] + [CRPsite_ptsGp1] ↔ [CRP_cAMP_CRPsite_ptsGp1];   {CRP_cAMP} , {CRPsite_ptsGp1} , {CRP_cAMP_CRPsite_ptsGp1}
 
 binding_CRP_cAMP_CRPsite_ptsGp2 [CRP_cAMP] + [CRPsite_ptsGp2] ↔ [CRP_cAMP_CRPsite_ptsGp2];   {CRP_cAMP} , {CRPsite_ptsGp2} , {CRP_cAMP_CRPsite_ptsGp2}
 
 binding_CRP_cAMP_CRPsite_ptsHp0 [CRP_cAMP] + [CRPsite_ptsHp0] ↔ [CRP_cAMP_CRPsite_ptsHp0];   {CRP_cAMP} , {CRPsite_ptsHp0} , {CRP_cAMP_CRPsite_ptsHp0}
 
 binding_CRP_cAMP_CRPsite_ptsHp1 [CRP_cAMP] + [CRPsite_ptsHp1] ↔ [CRP_cAMP_CRPsite_ptsHp1];   {CRP_cAMP} , {CRPsite_ptsHp1} , {CRP_cAMP_CRPsite_ptsHp1}
 
 binding_CRP_cAMP_CRPsite_ptsIp0 [CRP_cAMP] + [CRPsite_ptsIp0] ↔ [CRP_cAMP_CRPsite_ptsIp0];   {CRP_cAMP} , {CRPsite_ptsIp0} , {CRP_cAMP_CRPsite_ptsIp0}
 
 binding_CRP_cAMP_CRPsite_ptsIp1 [CRP_cAMP] + [CRPsite_ptsIp1] ↔ [CRP_cAMP_CRPsite_ptsIp1];   {CRP_cAMP} , {CRPsite_ptsIp1} , {CRP_cAMP_CRPsite_ptsIp1}
 
 binding_CRP_cAMP_CRPsite_mlcp1 [CRP_cAMP] + [CRPsite_mlcp1] ↔ [CRP_cAMP_CRPsite_mlcp1];   {CRP_cAMP} , {CRPsite_mlcp1} , {CRP_cAMP_CRPsite_mlcp1}
 
 binding_CRP_cAMP_CRPsite_mlcp2 [CRP_cAMP] + [CRPsite_mlcp2] ↔ [CRP_cAMP_CRPsite_mlcp2];   {CRP_cAMP} , {CRPsite_mlcp2} , {CRP_cAMP_CRPsite_mlcp2}
 
 binding_CRP_cAMP_CRPsite_genome [CRP_cAMP] + [CRPsite_genome] ↔ [CRP_cAMP_CRPsite_genome];   {CRP_cAMP} , {CRPsite_genome} , {CRP_cAMP_CRPsite_genome}
 
 binding_Mlc_Mlcsite_ptsGp1 [Mlc] + [Mlcsite_ptsGp1] ↔ [Mlc_Mlcsite_ptsGp1];   {Mlc} , {Mlcsite_ptsGp1} , {Mlc_Mlcsite_ptsGp1}
 
 binding_Mlc_Mlcsite_ptsGp2 [Mlc] + [Mlcsite_ptsGp2] ↔ [Mlc_Mlcsite_ptsGp2];   {Mlc} , {Mlcsite_ptsGp2} , {Mlc_Mlcsite_ptsGp2}
 
 binding_Mlc_Mlcsite_ptsHp0 [Mlc] + [Mlcsite_ptsHp0] ↔ [Mlc_Mlcsite_ptsHp0];   {Mlc} , {Mlcsite_ptsHp0} , {Mlc_Mlcsite_ptsHp0}
 
 binding_Mlc_Mlcsite_ptsIp0 [Mlc] + [Mlcsite_ptsIp0] ↔ [Mlc_Mlcsite_ptsIp0];   {Mlc} , {Mlcsite_ptsIp0} , {Mlc_Mlcsite_ptsIp0}
 
 binding_Mlc_Mlcsite_mlcp1 [Mlc] + [Mlcsite_mlcp1] ↔ [Mlc_Mlcsite_mlcp1];   {Mlc} , {Mlcsite_mlcp1} , {Mlc_Mlcsite_mlcp1}
 
 binding_Mlc_Mlcsite_mlcp2 [Mlc] + [Mlcsite_mlcp2] ↔ [Mlc_Mlcsite_mlcp2];   {Mlc} , {Mlcsite_mlcp2} , {Mlc_Mlcsite_mlcp2}
 
 binding_IICB_Mlc [IICB] + [Mlc] ↔ [IICB_Mlc];   {IICB} , {Mlc} , {IICB_Mlc}
 
 binding_IIA_P_CYA [CYA] + [IIA_P] ↔ [IIA_P_CYA];   {CYA} , {IIA_P} , {IIA_P_CYA}
 
 transcription_CRP_cAMP_CRPsite_cyaA_cyaA  ↔ [mRNA_cyaA];   {CRP_cAMP_CRPsite_cyaA} , {cyaA} , {CRP_cAMP_CRPsite_cyaA} , {cyaA}
 
 transcription_cyaA_basal  ↔ [mRNA_cyaA];   {cyaA_basal} , {cyaA_basal}
 
 transcription_CRP_cAMP_CRPsiteI_crp_CRP_cAMP_CRPsiteII_crp_crp  ↔ [mRNA_crp];   {CRP_cAMP_CRPsiteI_crp} , {CRP_cAMP_CRPsiteII_crp} , {crp} , {CRP_cAMP_CRPsiteII_crp} , {CRP_cAMP_CRPsiteI_crp} , {crp}
 
 transcription_crp_basal  ↔ [mRNA_crp];   {crp_basal} , {crp_basal}
 
 transcription_CRP_cAMP_CRPsite_ptsGp1_Mlc_Mlcsite_ptsGp1_ptsGp1  ↔ [mRNA_ptsG];   {CRP_cAMP_CRPsite_ptsGp1} , {Mlc_Mlcsite_ptsGp1} , {ptsGp1} , {CRP_cAMP_CRPsite_ptsGp1} , {Mlc_Mlcsite_ptsGp1} , {ptsGp1}
 
 transcription_CRP_cAMP_CRPsite_ptsGp2_Mlc_Mlcsite_ptsGp2_ptsGp2  ↔ [mRNA_ptsG];   {CRP_cAMP_CRPsite_ptsGp2} , {Mlc_Mlcsite_ptsGp2} , {ptsGp2} , {CRP_cAMP_CRPsite_ptsGp2} , {Mlc_Mlcsite_ptsGp2} , {ptsGp2}
 
 transcription_CRP_cAMP_CRPsite_ptsHp0_Mlc_Mlcsite_ptsHp0_ptsHp0  ↔ [mRNA_ptsH];   {CRP_cAMP_CRPsite_ptsHp0} , {Mlc_Mlcsite_ptsHp0} , {ptsHp0} , {CRP_cAMP_CRPsite_ptsHp0} , {Mlc_Mlcsite_ptsHp0} , {ptsHp0}
 
 transcription_CRP_cAMP_CRPsite_ptsHp1_ptsHp1  ↔ [mRNA_ptsH];   {CRP_cAMP_CRPsite_ptsHp1} , {ptsHp1} , {CRP_cAMP_CRPsite_ptsHp1} , {ptsHp1}
 
 transcription_CRP_cAMP_CRPsite_ptsIp0_Mlc_Mlcsite_ptsIp0_ptsIp0  ↔ [mRNA_ptsI];   {CRP_cAMP_CRPsite_ptsIp0} , {Mlc_Mlcsite_ptsIp0} , {ptsIp0} , {CRP_cAMP_CRPsite_ptsIp0} , {Mlc_Mlcsite_ptsIp0} , {ptsIp0}
 
 transcription_CRP_cAMP_CRPsite_ptsIp1_ptsIp1  ↔ [mRNA_ptsI];   {CRP_cAMP_CRPsite_ptsIp1} , {ptsIp1} , {CRP_cAMP_CRPsite_ptsIp1} , {ptsIp1}
 
 transcription_crr  ↔ [mRNA_crr];   {crr} , {crr}
 
 transcription_CRP_cAMP_CRPsite_mlcp1_Mlc_Mlcsite_mlcp1_mlcp1  ↔ [mRNA_mlc];   {CRP_cAMP_CRPsite_mlcp1} , {Mlc_Mlcsite_mlcp1} , {mlcp1} , {CRP_cAMP_CRPsite_mlcp1} , {Mlc_Mlcsite_mlcp1} , {mlcp1}
 
 transcription_CRP_cAMP_CRPsite_mlcp2_Mlc_Mlcsite_mlcp2_mlcp2  ↔ [mRNA_mlc];   {CRP_cAMP_CRPsite_mlcp2} , {Mlc_Mlcsite_mlcp2} , {mlcp2} , {CRP_cAMP_CRPsite_mlcp2} , {Mlc_Mlcsite_mlcp2} , {mlcp2}
 
 decomposition_mRNA_cyaA [mRNA_cyaA] ↔ ;   {mRNA_cyaA}
 
 decomposition_mRNA_crp [mRNA_crp] ↔ ;   {mRNA_crp}
 
 decomposition_mRNA_ptsG [mRNA_ptsG] ↔ ;   {mRNA_ptsG}
 
 decomposition_mRNA_ptsH [mRNA_ptsH] ↔ ;   {mRNA_ptsH}
 
 decomposition_mRNA_ptsI [mRNA_ptsI] ↔ ;   {mRNA_ptsI}
 
 decomposition_mRNA_crr [mRNA_crr] ↔ ;   {mRNA_crr}
 
 decomposition_mRNA_mlc [mRNA_mlc] ↔ ;   {mRNA_mlc}
 
 translation_mRNA_cyaA  ↔ [CYA];   {mRNA_cyaA} , {mRNA_cyaA}
 
 translation_mRNA_crp  ↔ [CRP];   {mRNA_crp} , {mRNA_crp}
 
 translation_mRNA_ptsG  ↔ [IICB];   {mRNA_ptsG} , {mRNA_ptsG}
 
 translation_mRNA_ptsH  ↔ [HPr];   {mRNA_ptsH} , {mRNA_ptsH}
 
 translation_mRNA_ptsI  ↔ [EI];   {mRNA_ptsI} , {mRNA_ptsI}
 
 translation_mRNA_crr  ↔ [IIA];   {mRNA_crr} , {mRNA_crr}
 
 translation_mlc  ↔ [Mlc];   {mRNA_mlc} , {mRNA_mlc}
 
 decomposition_CYA [CYA] ↔ ;   {CYA}
 
 decomposition_CRP [CRP] ↔ ;   {CRP}
 
 decomposition_Mlc [Mlc] ↔ ;   {Mlc}
 
 decomposition_cAMP [cAMP] ↔ ;   {cAMP}
 
 decomposition_CRP_cAMP [CRP_cAMP] ↔ ;   {CRP_cAMP}
 
 decomposition_CRP_cAMP_CRPsite_cyaA [CRP_cAMP_CRPsite_cyaA] ↔ [CRPsite_cyaA];   {CRP_cAMP_CRPsite_cyaA}
 
 decomposition_CRP_cAMP_CRPsiteI_crp [CRP_cAMP_CRPsiteI_crp] ↔ [CRPsiteI_crp];   {CRP_cAMP_CRPsiteI_crp}
 
 decomposition_CRP_cAMP_CRPsiteII_crp [CRP_cAMP_CRPsiteII_crp] ↔ [CRPsiteII_crp];   {CRP_cAMP_CRPsiteII_crp}
 
 decomposition_CRP_cAMP_CRPsite_ptsGp1 [CRP_cAMP_CRPsite_ptsGp1] ↔ [CRPsite_ptsGp1];   {CRP_cAMP_CRPsite_ptsGp1}
 
 decomposition_CRP_cAMP_CRPsite_ptsGp2 [CRP_cAMP_CRPsite_ptsGp2] ↔ [CRPsite_ptsGp2];   {CRP_cAMP_CRPsite_ptsGp2}
 
 decomposition_CRP_cAMP_CRPsite_ptsHp0 [CRP_cAMP_CRPsite_ptsHp0] ↔ [CRPsite_ptsHp0];   {CRP_cAMP_CRPsite_ptsHp0}
 
 decomposition_CRP_cAMP_CRPsite_ptsHp1 [CRP_cAMP_CRPsite_ptsHp1] ↔ [CRPsite_ptsHp1];   {CRP_cAMP_CRPsite_ptsHp1}
 
 decomposition_CRP_cAMP_CRPsite_ptsIp0 [CRP_cAMP_CRPsite_ptsIp0] ↔ [CRPsite_ptsIp0];   {CRP_cAMP_CRPsite_ptsIp0}
 
 decomposition_CRP_cAMP_CRPsite_ptsIp1 [CRP_cAMP_CRPsite_ptsIp1] ↔ [CRPsite_ptsIp1];   {CRP_cAMP_CRPsite_ptsIp1}
 
 decomposition_CRP_cAMP_CRPsite_mlcp1 [CRP_cAMP_CRPsite_mlcp1] ↔ [CRPsite_mlcp1];   {CRP_cAMP_CRPsite_mlcp1}
 
 decomposition_CRP_cAMP_CRPsite_mlcp2 [CRP_cAMP_CRPsite_mlcp2] ↔ [CRPsite_mlcp2];   {CRP_cAMP_CRPsite_mlcp2}
 
 decomposition_CRP_cAMP_CRPsite_genome [CRP_cAMP_CRPsite_genome] ↔ [CRPsite_genome];   {CRP_cAMP_CRPsite_genome}
 
 decomposition_Mlc_Mlcsite_ptsGp1 [Mlc_Mlcsite_ptsGp1] ↔ [Mlcsite_ptsGp1];   {Mlc_Mlcsite_ptsGp1}
 
 decomposition_Mlc_Mlcsite_ptsGp2 [Mlc_Mlcsite_ptsGp2] ↔ [Mlcsite_ptsGp2];   {Mlc_Mlcsite_ptsGp2}
 
 decomposition_Mlc_Mlcsite_ptsHp0 [Mlc_Mlcsite_ptsHp0] ↔ [Mlcsite_ptsHp0];   {Mlc_Mlcsite_ptsHp0}
 
 decomposition_Mlc_Mlcsite_ptsIp0 [Mlc_Mlcsite_ptsIp0] ↔ [Mlcsite_ptsIp0];   {Mlc_Mlcsite_ptsIp0}
 
 decomposition_Mlc_Mlcsite_mlcp1 [Mlc_Mlcsite_mlcp1] ↔ [Mlcsite_mlcp1];   {Mlc_Mlcsite_mlcp1}
 
 decomposition_Mlc_Mlcsite_mlcp2 [Mlc_Mlcsite_mlcp2] ↔ [Mlcsite_mlcp2];   {Mlc_Mlcsite_mlcp2}
 
 decomposition_IICB_Mlc [IICB_Mlc] ↔ ;   {IICB_Mlc}
 
 decomposition_EI_P [EI_P] ↔ ;   {EI_P}
 
 decomposition_EI [EI] ↔ ;   {EI}
 
 decomposition_HPr_P [HPr_P] ↔ ;   {HPr_P}
 
 decomposition_HPr [HPr] ↔ ;   {HPr}
 
 decomposition_IIA_P [IIA_P] ↔ ;   {IIA_P}
 
 decomposition_IIA [IIA] ↔ ;   {IIA}
 
 decomposition_IICB_P [IICB_P] ↔ ;   {IICB_P}
 
 decomposition_IICB [IICB] ↔ ;   {IICB}
 
 PTS2for [HPr] + [EI_P] ↔ [HPr_P] + [EI];   {HPr} , {EI_P}
 
 PTS2rev [HPr_P] + [EI] ↔ [HPr] + [EI_P];   {EI} , {HPr_P}
 
 PTS3for [IIA] + [HPr_P] ↔ [IIA_P] + [HPr];   {IIA} , {HPr_P}
 
 PTS3rev [IIA_P] + [HPr] ↔ [IIA] + [HPr_P];   {HPr} , {IIA_P}
 
 PTS4for [IICB] + [IIA_P] ↔ [IICB_P] + [IIA];   {IICB} , {IIA_P}
 
 PTS4rev [IICB_P] + [IIA] ↔ [IICB] + [IIA_P];   {IIA} , {IICB_P}
 
 reaction_CYA_ATP [ATP] ↔ [cAMP];   {CYA} , {CYA} , {ATP}
 
 reaction_IIA_P_CYA_ATP [ATP] ↔ [cAMP];   {IIA_P_CYA} , {IIA_P_CYA} , {ATP}
 
 reaction_EI_PEP [EI] + [PEP] ↔ [EI_P] + [Pyr];   {EI} , {PEP}
 
 reaction_EIP_Pyr [EI_P] + [Pyr] ↔ [EI] + [PEP];   {EI_P} , {Pyr}
 
 reaction_IICB_P_Glucose [IICB_P] + [Glucose] ↔ [IICB] + [Glc6P];   {IICB_P} , {Glucose}
 
 reaction_IICB_Glc6P [IICB] + [Glc6P] ↔ [IICB_P] + [Glucose];   {IICB} , {Glc6P}
 
Rules (17)
 
 Assignment Rule (name: TCRPsite_cyaA) TCRPsite_cyaA = CRPsite_cyaA+CRP_cAMP_CRPsite_cyaA
 
 Assignment Rule (name: TCRPsiteI_crp) TCRPsiteI_crp = CRPsiteI_crp+CRP_cAMP_CRPsiteI_crp
 
 Assignment Rule (name: TCRPsiteII_crp) TCRPsiteII_crp = CRPsiteII_crp+CRP_cAMP_CRPsiteII_crp
 
 Assignment Rule (name: TCRPsite_ptsGp1) TCRPsite_ptsGp1 = CRPsite_ptsGp1+CRP_cAMP_CRPsite_ptsGp1
 
 Assignment Rule (name: TMlcsite_ptsGp1) TMlcsite_ptsGp1 = Mlcsite_ptsGp1+Mlc_Mlcsite_ptsGp1
 
 Assignment Rule (name: TCRPsite_ptsGp2) TCRPsite_ptsGp2 = CRPsite_ptsGp2+CRP_cAMP_CRPsite_ptsGp2
 
 Assignment Rule (name: TMlcsite_ptsGp2) TMlcsite_ptsGp2 = Mlcsite_ptsGp2+Mlc_Mlcsite_ptsGp2
 
 Assignment Rule (name: TCRPsite_ptsHp0) TCRPsite_ptsHp0 = CRPsite_ptsHp0+CRP_cAMP_CRPsite_ptsHp0
 
 Assignment Rule (name: TMlcsite_ptsHp0) TMlcsite_ptsHp0 = Mlcsite_ptsHp0+Mlc_Mlcsite_ptsHp0
 
 Assignment Rule (name: TCRPsite_ptsHp1) TCRPsite_ptsHp1 = CRPsite_ptsHp1+CRP_cAMP_CRPsite_ptsHp1
 
 Assignment Rule (name: TCRPsite_ptsIp0) TCRPsite_ptsIp0 = CRPsite_ptsIp0+CRP_cAMP_CRPsite_ptsIp0
 
 Assignment Rule (name: TMlcsite_ptsIp0) TMlcsite_ptsIp0 = Mlcsite_ptsIp0+Mlc_Mlcsite_ptsIp0
 
 Assignment Rule (name: TCRPsite_ptsIp1) TCRPsite_ptsIp1 = CRPsite_ptsIp1+CRP_cAMP_CRPsite_ptsIp1
 
 Assignment Rule (name: TCRPsite_mlcp1) TCRPsite_mlcp1 = CRPsite_mlcp1+CRP_cAMP_CRPsite_mlcp1
 
 Assignment Rule (name: TMlcsite_mlcp1) TMlcsite_mlcp1 = Mlcsite_mlcp1+Mlc_Mlcsite_mlcp1
 
 Assignment Rule (name: TCRPsite_mlcp2) TCRPsite_mlcp2 = CRPsite_mlcp2+CRP_cAMP_CRPsite_mlcp2
 
 Assignment Rule (name: TMlcsite_mlcp2) TMlcsite_mlcp2 = Mlcsite_mlcp2+Mlc_Mlcsite_mlcp2
 
Events (1)
 
  
Glucose = 2E-9
 
  Spatial dimensions: 3.0  Compartment size: 1.0
 
 CRP
Compartment: cyt
Initial concentration: 5.4207E-6
 
 CRPsiteI_crp
Compartment: cyt
Initial concentration: 7.4368E-11
 
 CRPsiteII_crp
Compartment: cyt
Initial concentration: 1.9047E-10
 
 CRPsite_cyaA
Compartment: cyt
Initial concentration: 3.1103E-11
 
 CRPsite_genome
Compartment: cyt
Initial concentration: 3.6756E-9
 
 CRPsite_ptsGp1
Compartment: cyt
Initial concentration: 1.2021E-10
 
 CRPsite_ptsGp2
Compartment: cyt
Initial concentration: 1.2021E-10
 
 CRPsite_ptsHp0
Compartment: cyt
Initial concentration: 1.2021E-10
 
 CRPsite_ptsHp1
Compartment: cyt
Initial concentration: 1.2021E-10
 
 CRPsite_ptsIp0
Compartment: cyt
Initial concentration: 1.2021E-10
 
 CRPsite_ptsIp1
Compartment: cyt
Initial concentration: 1.2021E-10
 
 CRPsite_mlcp1
Compartment: cyt
Initial concentration: 1.2021E-10
 
 CRPsite_mlcp2
Compartment: cyt
Initial concentration: 1.2021E-10
 
 Mlc
Compartment: cyt
Initial concentration: 5.5172E-10
 
 Mlcsite_mlcp1
Compartment: cyt
Initial concentration: 2.4267E-10
 
 Mlcsite_mlcp2
Compartment: cyt
Initial concentration: 2.4282E-10
 
 Mlcsite_ptsGp1
Compartment: cyt
Initial concentration: 2.1885E-10
 
 Mlcsite_ptsGp2
Compartment: cyt
Initial concentration: 2.1885E-10
 
 Mlcsite_ptsHp0
Compartment: cyt
Initial concentration: 2.1885E-10
 
 Mlcsite_ptsIp0
Compartment: cyt
Initial concentration: 2.1885E-10
 
 CRP_cAMP
Compartment: cyt
Initial concentration: 1.0214E-7
 
 CRP_cAMP_CRPsiteI_crp
Compartment: cyt
Initial concentration: 1.6863E-10
 
 CRP_cAMP_CRPsiteII_crp
Compartment: cyt
Initial concentration: 5.2529E-11
 
 CRP_cAMP_CRPsite_cyaA
Compartment: cyt
Initial concentration: 2.119E-10
 
 CRP_cAMP_CRPsite_genome
Compartment: cyt
Initial concentration: 3.7544E-9
 
 CRP_cAMP_CRPsite_ptsGp1
Compartment: cyt
Initial concentration: 1.2279E-10
 
 CRP_cAMP_CRPsite_ptsGp2
Compartment: cyt
Initial concentration: 1.2279E-10
 
 CRP_cAMP_CRPsite_ptsHp0
Compartment: cyt
Initial concentration: 1.2279E-10
 
 CRP_cAMP_CRPsite_ptsHp1
Compartment: cyt
Initial concentration: 1.2279E-10
 
 CRP_cAMP_CRPsite_ptsIp0
Compartment: cyt
Initial concentration: 1.2279E-10
 
 CRP_cAMP_CRPsite_ptsIp1
Compartment: cyt
Initial concentration: 1.2279E-10
 
 CRP_cAMP_CRPsite_mlcp1
Compartment: cyt
Initial concentration: 1.2279E-10
 
 CRP_cAMP_CRPsite_mlcp2
Compartment: cyt
Initial concentration: 1.2279E-10
 
 Mlc_Mlcsite_ptsGp1
Compartment: cyt
Initial concentration: 2.4149E-11
 
 Mlc_Mlcsite_ptsGp2
Compartment: cyt
Initial concentration: 2.4149E-11
 
 Mlc_Mlcsite_ptsIp0
Compartment: cyt
Initial concentration: 2.4149E-11
 
 Mlc_Mlcsite_ptsHp0
Compartment: cyt
Initial concentration: 2.4149E-11
 
 Mlc_Mlcsite_mlcp1
Compartment: cyt
Initial concentration: 3.2535E-13
 
 Mlc_Mlcsite_mlcp2
Compartment: cyt
Initial concentration: 1.8086E-13
 
 IICB
Compartment: cyt
Initial concentration: 4.2844E-5
 
 IICB_Mlc
Compartment: cyt
Initial concentration: 1.6546E-7
 
 CYA
Compartment: cyt
Initial concentration: 1.4934E-6
 
 IIA_P
Compartment: cyt
Initial concentration: 7.0094E-6
 
 IIA_P_CYA
Compartment: cyt
Initial concentration: 7.3371E-9
 
 mRNA_cyaA
Compartment: cyt
Initial concentration: 1.3643E-8
 
 mRNA_crp
Compartment: cyt
Initial concentration: 5.0254E-8
 
 mRNA_ptsG
Compartment: cyt
Initial concentration: 4.5559E-7
 
 mRNA_ptsH
Compartment: cyt
Initial concentration: 1.1411E-7
 
 mRNA_ptsI
Compartment: cyt
Initial concentration: 1.0038E-8
 
 mRNA_crr
Compartment: cyt
Initial concentration: 9.3861E-7
 
 mRNA_mlc
Compartment: cyt
Initial concentration: 1.5101E-9
 
 IICB_P
Compartment: cyt
Initial concentration: 7.1055E-6
 
 IIA
Compartment: cyt
Initial concentration: 9.623E-5
 
 HPr_P
Compartment: cyt
Initial concentration: 7.5867E-7
 
 HPr
Compartment: cyt
Initial concentration: 1.1793E-5
 
 EI_P
Compartment: cyt
Initial concentration: 2.4319E-7
 
 EI
Compartment: cyt
Initial concentration: 8.6098E-7
 
 cAMP
Compartment: cyt
Initial concentration: 4.7107E-7
 
 cyaA
Compartment: cyt
Initial concentration: 2.43E-10
Constant
 
 cyaA_basal
Compartment: cyt
Initial concentration: 2.43E-10
Constant
 
 crp
Compartment: cyt
Initial concentration: 2.43E-10
Constant
 
 crp_basal
Compartment: cyt
Initial concentration: 2.43E-10
Constant
 
 ptsGp1
Compartment: cyt
Initial concentration: 2.43E-10
Constant
 
 ptsGp2
Compartment: cyt
Initial concentration: 2.43E-10
Constant
 
 ptsHp0
Compartment: cyt
Initial concentration: 2.43E-10
Constant
 
 ptsHp1
Compartment: cyt
Initial concentration: 2.43E-10
Constant
 
 ptsIp0
Compartment: cyt
Initial concentration: 2.43E-10
Constant
 
 ptsIp1
Compartment: cyt
Initial concentration: 2.43E-10
Constant
 
 crr
Compartment: cyt
Initial concentration: 2.43E-10
Constant
 
 mlcp1
Compartment: cyt
Initial concentration: 2.43E-10
Constant
 
 mlcp2
Compartment: cyt
Initial concentration: 2.43E-10
Constant
 
 Pyr
Compartment: cyt
Initial concentration: 0.00267
Constant
 
 PEP
Compartment: cyt
Initial concentration: 0.00267
Constant
 
 Glc6P
Compartment: cyt
Initial concentration: 0.00148
Constant
 
 Glucose
Compartment: cyt
Initial concentration: 0.2
 
 ATP
Compartment: cyt
Initial concentration: 0.0069942
Constant
 
Global Parameters (18)
 
   fast
Value: 1.0E9   (Units: per_min)
Constant
 
   TCRPsite_cyaA
Value: 2.43E-10   (Units: M)
 
   TCRPsiteI_crp
Value: 2.43E-10   (Units: M)
 
   TCRPsiteII_crp
Value: 2.43E-10   (Units: M)
 
   TCRPsite_ptsGp1
Value: 2.43E-10   (Units: M)
 
   TMlcsite_ptsGp1
Value: 2.43E-10   (Units: M)
 
   TCRPsite_ptsGp2
Value: 2.43E-10   (Units: M)
 
   TMlcsite_ptsGp2
Value: 2.43E-10   (Units: M)
 
   TCRPsite_ptsHp0
Value: 2.43E-10   (Units: M)
 
   TMlcsite_ptsHp0
Value: 2.43E-10   (Units: M)
 
   TCRPsite_ptsHp1
Value: 2.43E-10   (Units: M)
 
   TCRPsite_ptsIp0
Value: 2.43E-10   (Units: M)
 
   TMlcsite_ptsIp0
Value: 2.43E-10   (Units: M)
 
   TCRPsite_ptsIp1
Value: 2.43E-10   (Units: M)
 
   TCRPsite_mlcp1
Value: 2.43E-10   (Units: M)
 
   TMlcsite_mlcp1
Value: 2.43E-10   (Units: M)
 
   TCRPsite_mlcp2
Value: 2.43E-10   (Units: M)
 
   TMlcsite_mlcp2
Value: 2.43E-10   (Units: M)
 
binding_CRP_cAMP (2)
 
   Kb
Value: 40000.0   (Units: per_M)
Constant
 
   one_per_M
Value: 1.0   (Units: per_M)
Constant
 
binding_CRP_cAMP_CRPsite_cyaA (1)
 
   Kb
Value: 6.67E7   (Units: per_M)
Constant
 
binding_CRP_cAMP_CRPsiteI_crp (1)
 
   Kb
Value: 2.22E7   (Units: per_M)
Constant
 
binding_CRP_cAMP_CRPsiteII_crp (1)
 
   Kb
Value: 2700000.0   (Units: per_M)
Constant
 
binding_CRP_cAMP_CRPsite_ptsGp1 (1)
 
   Kb
Value: 1.0E7   (Units: per_M)
Constant
 
binding_CRP_cAMP_CRPsite_ptsGp2 (1)
 
   Kb
Value: 1.0E7   (Units: per_M)
Constant
 
binding_CRP_cAMP_CRPsite_ptsHp0 (1)
 
   Kb
Value: 1.0E7   (Units: per_M)
Constant
 
binding_CRP_cAMP_CRPsite_ptsHp1 (1)
 
   Kb
Value: 1.0E7   (Units: per_M)
Constant
 
binding_CRP_cAMP_CRPsite_ptsIp0 (1)
 
   Kb
Value: 1.0E7   (Units: per_M)
Constant
 
binding_CRP_cAMP_CRPsite_ptsIp1 (1)
 
   Kb
Value: 1.0E7   (Units: per_M)
Constant
 
binding_CRP_cAMP_CRPsite_mlcp1 (1)
 
   Kb
Value: 1.0E7   (Units: per_M)
Constant
 
binding_CRP_cAMP_CRPsite_mlcp2 (1)
 
   Kb
Value: 1.0E7   (Units: per_M)
Constant
 
binding_CRP_cAMP_CRPsite_genome (1)
 
   Kb
Value: 1.0E7   (Units: per_M)
Constant
 
binding_Mlc_Mlcsite_ptsGp1 (1)
 
   Kb
Value: 2.0E8   (Units: per_M)
Constant
 
binding_Mlc_Mlcsite_ptsGp2 (1)
 
   Kb
Value: 2.0E8   (Units: per_M)
Constant
 
binding_Mlc_Mlcsite_ptsHp0 (1)
 
   Kb
Value: 2.0E8   (Units: per_M)
Constant
 
binding_Mlc_Mlcsite_ptsIp0 (1)
 
   Kb
Value: 2.0E8   (Units: per_M)
Constant
 
binding_Mlc_Mlcsite_mlcp1 (1)
 
   Kb
Value: 2430000.0   (Units: per_M)
Constant
 
binding_Mlc_Mlcsite_mlcp2 (1)
 
   Kb
Value: 1350000.0   (Units: per_M)
Constant
 
binding_IICB_Mlc (1)
 
   Kb
Value: 7000000.0   (Units: per_M)
Constant
 
binding_IIA_P_CYA (1)
 
   Kb
Value: 1.0E8   (Units: per_M_squared)
Constant
 
transcription_CRP_cAMP_CRPsite_cyaA_cyaA (1)
 
   km
Value: 45.26   (Units: per_min)
Constant
 
transcription_cyaA_basal (1)
 
   km
Value: 1.281   (Units: per_min)
Constant
 
transcription_CRP_cAMP_CRPsiteI_crp_CRP_cAMP_CRPsiteII_crp_crp (2)
 
   km
Value: 20.0   (Units: per_min)
Constant
 
   RelativeactivityatTCRPsiteII_crp
Value: 5.0   (Units: dimensionless)
Constant
 
transcription_crp_basal (1)
 
   km
Value: 1.00886   (Units: per_min)
Constant
 
transcription_CRP_cAMP_CRPsite_ptsGp1_Mlc_Mlcsite_ptsGp1_ptsGp1 (1)
 
   km
Value: 892.0   (Units: per_min)
Constant
 
transcription_CRP_cAMP_CRPsite_ptsGp2_Mlc_Mlcsite_ptsGp2_ptsGp2 (1)
 
   km
Value: 2.0   (Units: per_min)
Constant
 
transcription_CRP_cAMP_CRPsite_ptsHp0_Mlc_Mlcsite_ptsHp0_ptsHp0 (1)
 
   km
Value: 71.8   (Units: per_min)
Constant
 
transcription_CRP_cAMP_CRPsite_ptsHp1_ptsHp1 (1)
 
   km
Value: 17.95   (Units: per_min)
Constant
 
transcription_CRP_cAMP_CRPsite_ptsIp0_Mlc_Mlcsite_ptsIp0_ptsIp0 (1)
 
   km
Value: 6.244   (Units: per_min)
Constant
 
transcription_CRP_cAMP_CRPsite_ptsIp1_ptsIp1 (1)
 
   km
Value: 0.892   (Units: per_min)
Constant
 
transcription_crr (1)
 
   km
Value: 334.5   (Units: per_min)
Constant
 
transcription_CRP_cAMP_CRPsite_mlcp1_Mlc_Mlcsite_mlcp1_mlcp1 (1)
 
   km
Value: 1.875   (Units: per_min)
Constant
 
transcription_CRP_cAMP_CRPsite_mlcp2_Mlc_Mlcsite_mlcp2_mlcp2 (1)
 
   km
Value: 1.875   (Units: per_min)
Constant
 
decomposition_mRNA_cyaA (1)
 
   kmd
Value: 0.126   (Units: per_min)
Constant
 
decomposition_mRNA_crp (1)
 
   kmd
Value: 0.139   (Units: per_min)
Constant
 
decomposition_mRNA_ptsG (1)
 
   kmd
Value: 0.217   (Units: per_min)
Constant
 
decomposition_mRNA_ptsH (1)
 
   kmd
Value: 0.0889   (Units: per_min)
Constant
 
decomposition_mRNA_ptsI (1)
 
   kmd
Value: 0.0797   (Units: per_min)
Constant
 
decomposition_mRNA_crr (1)
 
   kmd
Value: 0.0866   (Units: per_min)
Constant
 
decomposition_mRNA_mlc (1)
 
   kmd
Value: 0.3014   (Units: per_min)
Constant
 
translation_mRNA_cyaA (1)
 
   kp
Value: 11.0   (Units: per_min)
Constant
 
translation_mRNA_crp (1)
 
   kp
Value: 11.0   (Units: per_min)
Constant
 
translation_mRNA_ptsG (1)
 
   kp
Value: 11.0   (Units: per_min)
Constant
 
translation_mRNA_ptsH (1)
 
   kp
Value: 11.0   (Units: per_min)
Constant
 
translation_mRNA_ptsI (1)
 
   kp
Value: 11.0   (Units: per_min)
Constant
 
translation_mRNA_crr (1)
 
   kp
Value: 11.0   (Units: per_min)
Constant
 
translation_mlc (1)
 
   kp
Value: 11.0   (Units: per_min)
Constant
 
decomposition_CYA (1)
 
   kpd
Value: 0.1   (Units: per_min)
Constant
 
decomposition_CRP (1)
 
   kpd
Value: 0.1   (Units: per_min)
Constant
 
decomposition_Mlc (1)
 
   kpd
Value: 0.1   (Units: per_min)
Constant
 
decomposition_cAMP (1)
 
   kpd
Value: 400.0   (Units: per_min)
Constant
 
decomposition_CRP_cAMP (1)
 
   kpd
Value: 0.1   (Units: per_min)
Constant
 
decomposition_CRP_cAMP_CRPsite_cyaA (1)
 
   kpd
Value: 0.1   (Units: per_min)
Constant
 
decomposition_CRP_cAMP_CRPsiteI_crp (1)
 
   kpd
Value: 0.1   (Units: per_min)
Constant
 
decomposition_CRP_cAMP_CRPsiteII_crp (1)
 
   kpd
Value: 0.1   (Units: per_min)
Constant
 
decomposition_CRP_cAMP_CRPsite_ptsGp1 (1)
 
   kpd
Value: 0.1   (Units: per_min)
Constant
 
decomposition_CRP_cAMP_CRPsite_ptsGp2 (1)
 
   kpd
Value: 0.1   (Units: per_min)
Constant
 
decomposition_CRP_cAMP_CRPsite_ptsHp0 (1)
 
   kpd
Value: 0.1   (Units: per_min)
Constant
 
decomposition_CRP_cAMP_CRPsite_ptsHp1 (1)
 
   kpd
Value: 0.1   (Units: per_min)
Constant
 
decomposition_CRP_cAMP_CRPsite_ptsIp0 (1)
 
   kpd
Value: 0.1   (Units: per_min)
Constant
 
decomposition_CRP_cAMP_CRPsite_ptsIp1 (1)
 
   kpd
Value: 0.1   (Units: per_min)
Constant
 
decomposition_CRP_cAMP_CRPsite_mlcp1 (1)
 
   kpd
Value: 0.1   (Units: per_min)
Constant
 
decomposition_CRP_cAMP_CRPsite_mlcp2 (1)
 
   kpd
Value: 0.1   (Units: per_min)
Constant
 
decomposition_CRP_cAMP_CRPsite_genome (1)
 
   kpd
Value: 0.1   (Units: per_min)
Constant
 
decomposition_Mlc_Mlcsite_ptsGp1 (1)
 
   kpd
Value: 0.1   (Units: per_min)
Constant
 
decomposition_Mlc_Mlcsite_ptsGp2 (1)
 
   kpd
Value: 0.1   (Units: per_min)
Constant
 
decomposition_Mlc_Mlcsite_ptsHp0 (1)
 
   kpd
Value: 0.1   (Units: per_min)
Constant
 
decomposition_Mlc_Mlcsite_ptsIp0 (1)
 
   kpd
Value: 0.1   (Units: per_min)
Constant
 
decomposition_Mlc_Mlcsite_mlcp1 (1)
 
   kpd
Value: 0.1   (Units: per_min)
Constant
 
decomposition_Mlc_Mlcsite_mlcp2 (1)
 
   kpd
Value: 0.1   (Units: per_min)
Constant
 
decomposition_IICB_Mlc (1)
 
   kpd
Value: 0.1   (Units: per_min)
Constant
 
decomposition_EI_P (1)
 
   kpd
Value: 0.1   (Units: per_min)
Constant
 
decomposition_EI (1)
 
   kpd
Value: 0.1   (Units: per_min)
Constant
 
decomposition_HPr_P (1)
 
   kpd
Value: 0.1   (Units: per_min)
Constant
 
decomposition_HPr (1)
 
   kpd
Value: 0.1   (Units: per_min)
Constant
 
decomposition_IIA_P (1)
 
   kpd
Value: 0.1   (Units: per_min)
Constant
 
decomposition_IIA (1)
 
   kpd
Value: 0.1   (Units: per_min)
Constant
 
decomposition_IICB_P (1)
 
   kpd
Value: 0.1   (Units: per_min)
Constant
 
decomposition_IICB (1)
 
   kpd
Value: 0.1   (Units: per_min)
Constant
 
PTS2for (1)
 
   kx
Value: 1.2E10   (Units: per_M_per_min)
Constant
 
PTS2rev (1)
 
   kx
Value: 4.8E8   (Units: per_M_per_min)
Constant
 
PTS3for (1)
 
   kx
Value: 3.66E9   (Units: per_M_per_min)
Constant
 
PTS3rev (1)
 
   kx
Value: 2.82E9   (Units: per_M_per_min)
Constant
 
PTS4for (1)
 
   kx
Value: 6.6E8   (Units: per_M_per_min)
Constant
 
PTS4rev (1)
 
   kx
Value: 2.4E8   (Units: per_M_per_min)
Constant
 
reaction_CYA_ATP (2)
 
   Kmich
Value: 0.001   (Units: M)
Constant
 
   Q
Value: 100.0   (Units: per_min)
Constant
 
reaction_IIA_P_CYA_ATP (2)
 
   Kmich
Value: 0.001   (Units: M)
Constant
 
   Q
Value: 9000.0   (Units: per_min)
Constant
 
reaction_EI_PEP (2)
 
   Kmich
Value: 3.0E-4   (Units: M)
Constant
 
   Q
Value: 108000.0   (Units: per_min)
Constant
 
reaction_EIP_Pyr (2)
 
   Kmich
Value: 0.002   (Units: M)
Constant
 
   Q
Value: 480000.0   (Units: per_min)
Constant
 
reaction_IICB_P_Glucose (2)
 
   Kmich
Value: 2.0E-5   (Units: M)
Constant
 
   Q
Value: 4800.0   (Units: per_min)
Constant
 
reaction_IICB_Glc6P (2)
 
   Kmich
Value: 9.61   (Units: M)
Constant
 
   Q
Value: 389.0   (Units: per_min)
Constant
 
Representative curation result(s)
Representative curation result(s) of BIOMD0000000571

Curator's comment: (updated: 25 Mar 2015 16:27:06 GMT)

Figure 3A of the reference publication has been reproduced here: simulated time course for IIAGlc protein in response to glucose depletion in the PTS for 1000 minutes.

The simulation was done using Copasi v4.14 (Build 89) and the plot was generated using Gnuplot. The Copasi file of the model with simulation settings for the whole Figure 3 can be downloaded from the below link:

Additional file(s)
  • Nishio2008 - Design of the phosphotransferase system for enhanced glucose uptake in E. coli.:
    Copasi file of the model
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