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BIOMD0000000457 - Firczuk2013 - Eukaryotic mRNA translation machinery

 

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Reference Publication
Publication ID: 23340841
Firczuk H, Kannambath S, Pahle J, Claydon A, Beynon R, Duncan J, Westerhoff H, Mendes P, McCarthy JE.
An in vivo control map for the eukaryotic mRNA translation machinery.
Mol. Syst. Biol. 2013; 9: 635
School of Life Sciences, University of Warwick, Coventry, UK.  [more]
Model
Original Model: BIOMD0000000457.xml.origin
Submitter: Pedro Mendes
Submission ID: MODEL1205190000
Submission Date: 19 May 2012 15:58:43 UTC
Last Modification Date: 21 Jun 2013 11:28:41 UTC
Creation Date: 30 Apr 2011 21:53:18 UTC
Encoders:  Pedro Mendes
   Shichina Kannambath
   Juergen Pahle
set #1
bqbiol:isPartOf Taxonomy Saccharomyces cerevisiae
bqbiol:isVersionOf Gene Ontology translation
Notes
Firczuk2013 - Eukaryotic mRNA translation machinery

This is a model of Saccharomyces cerevisiae mRNA translation which includes the initiation, elongation and termination phases. The model is for 20 condon mRNAs. The building of a multi-factor complex in initiation and also the different processes in elongation and termination are modelled in detail. The model takes into account that ribosomes cover more than one codon of mRNA so that the movement of ribosomes are effectively blocked by other ribosomes several codons downstream. It is assumed that 15 codons are occupied by each ribosome. This blocking effect is considered in reaction R18 in initiation and also reaction R26, the reaction where translocation of ribosomes takes place in elongation. The kinetic functions of these two reactions are based on MacDonald et al. 1968 and Heinrich & Rapaport 1980. All other kinetic functions follow mass-action kinetics. The concentrations of transfer RNA species (Met-tRNA, aa-tRNA and tRNA in the model) are kept constant, while the other species' concentrations can change in the course of the simulation. The model describes the translation of a short mRNA with 20 codons. Therefore, all reactions in the elongation cycle (R22, R23, R25, R26, R28 and R29) and the corresponding species are replicated accordingly to model the species with ribosomes bound at different positions. In summary, the model contains 165 different species and 141 reactions.

The value of the 56 rate constant parameters were estimated by fitting the model against a series of experimental data consisting of modulation of the various translation factors (Figures 2, 3 and S3). Overall the parameter estimation was carried out over 212 different data points (steady states).

This model is described in the article:

Helena Firczuk, Shichina Kannambath, Jürgen Pahle, Amy Claydon, Robert Beynon, John Duncan, Hans Westerhoff, Pedro Mendes and John EG McCarthy
Molecular Systems Biology. 9:635

Abstract:

Rate control analysis defines the in vivo control map governing yeast protein synthesis and generates an extensively parameterized digital model of the translation pathway. Among other non-intuitive outcomes, translation demonstrates a high degree of functional modularity and comprises a non-stoichiometric combination of proteins manifesting functional convergence on a shared maximal translation rate. In exponentially growing cells, polypeptide elongation (eEF1A, eEF2, and eEF3) exerts the strongest control. The two other strong control points are recruitment of mRNA and tRNAi to the 40S ribosomal subunit (eIF4F and eIF2) and termination (eRF1; Dbp5). In contrast, factors that are found to promote mRNA scanning efficiency on a longer than-average 5′untranslated region (eIF1, eIF1A, Ded1, eIF2B, eIF3, and eIF5) exceed the levels required for maximal control. This is expected to allow the cell to minimize scanning transition times, particularly for longer 5′UTRs. The analysis reveals these and other collective adaptations of control shared across the factors, as well as features that reflect functional modularity and system robustness. Remarkably, gene duplication is implicated in the fine control of cellular protein synthesis.

To the extent possible under law, all copyright and related or neighbouring rights to this encoded model have been dedicated to the public domain worldwide. Please refer to CC0 Public Domain Dedication for more information.

Model
Publication ID: 23340841 Submission Date: 19 May 2012 15:58:43 UTC Last Modification Date: 21 Jun 2013 11:28:41 UTC Creation Date: 30 Apr 2011 21:53:18 UTC
Mathematical expressions
Reactions
reaction1 reaction2 reaction3 reaction4
reaction5 reaction6 reaction7 reaction8
reaction9 reaction10 reaction11 reaction12
reaction13 reaction14 reaction15 reaction16
reaction17 reaction18 reaction19 reaction20
reaction21 reaction24 reaction27 reaction22_1
reaction23_1 reaction25_1 reaction26_1 reaction22_2
reaction23_2 reaction25_2 reaction26_2 reaction28_2
reaction29_2 reaction22_3 reaction23_3 reaction25_3
reaction26_3 reaction28_3 reaction29_3 reaction22_4
reaction23_4 reaction25_4 reaction26_4 reaction28_4
reaction29_4 reaction22_5 reaction23_5 reaction25_5
reaction26_5 reaction28_5 reaction29_5 reaction22_6
reaction23_6 reaction25_6 reaction26_6 reaction28_6
reaction29_6 reaction22_7 reaction23_7 reaction25_7
reaction26_7 reaction28_7 reaction29_7 reaction22_8
reaction23_8 reaction25_8 reaction26_8 reaction28_8
reaction29_8 reaction22_9 reaction23_9 reaction25_9
reaction26_9 reaction28_9 reaction29_9 reaction22_10
reaction23_10 reaction25_10 reaction26_10 reaction28_10
reaction29_10 reaction22_11 reaction23_11 reaction25_11
reaction26_11 reaction28_11 reaction29_11 reaction22_12
reaction23_12 reaction25_12 reaction26_12 reaction28_12
reaction29_12 reaction22_13 reaction23_13 reaction25_13
reaction26_13 reaction28_13 reaction29_13 reaction22_14
reaction23_14 reaction25_14 reaction26_14 reaction28_14
reaction29_14 reaction22_15 reaction23_15 reaction25_15
reaction26_15 reaction28_15 reaction29_15 reaction22_16
reaction23_16 reaction25_16 reaction26_16 reaction28_16
reaction29_16 reaction22_17 reaction23_17 reaction25_17
reaction26_17 reaction28_17 reaction29_17 reaction22_18
reaction23_18 reaction25_18 reaction26_18 reaction28_18
reaction29_18 reaction22_19 reaction23_19 reaction25_19
reaction26_19 reaction28_19 reaction29_19 reaction28_20
reaction29_20 reaction30 reaction31 reaction32
reaction33      
Rules
Assignment Rule (variable: mRNA_bound) Assignment Rule (variable: mRNA_free)    
Physical entities
Compartments Species
cytoplasm eIF2_GDP eIF2B eIF2_GDP_eIF2B
eIF2_GTP Met-tRNA eIF2_GTP_Met-tRNA
eIF3 eIF5 eIF3_eIF5
eIF3_eIF5_eIF2_GTP_Met-tRNA eIF1 eIF1_eIF3_eIF5_eIF2_GTP_Met-tRNA
40S eIF1A 40S_eIF1A
43S eIF4E eIF4G
eIF4E_eIF4G mRNA_cap Pab1
mRNA_Pab1 eIF4E_eIF4G_mRNA_Pab1 eIF4A
eIF4B eIF4A_eIF4B eIF4E_eIF4G_mRNA_Pab1_eIF4A_eIF4B
48S Ded1 48S_Ded1
eIF5B_GDP eIF5B_GTP 48S_Ded1_eIF5B_GTP
60S eEF1A_GDP eEF1B
eEF1A_GDP_eEF1B eEF1A_GTP aa-tRNA
aa-tRNA_eEF1A_GTP eEF2_GDP eEF2_GTP
eEF3_GDP eEF3_GTP tRNA
80S_1 80S_aa-tRNA_eEF1A_GTP_1 80S_aa-tRNA_1
80S_aa-tRNA_eEF2_GTP_1 80S_2 80S_aa-tRNA_eEF1A_GTP_2
80S_aa-tRNA_2 80S_aa-tRNA_eEF2_GTP_2 80S_tRNA_2
80S_tRNA_eEF3_GTP_2 80S_3 80S_aa-tRNA_eEF1A_GTP_3
80S_aa-tRNA_3 80S_aa-tRNA_eEF2_GTP_3 80S_tRNA_3
80S_tRNA_eEF3_GTP_3 80S_4 80S_aa-tRNA_eEF1A_GTP_4
80S_aa-tRNA_4 80S_aa-tRNA_eEF2_GTP_4 80S_tRNA_4
80S_tRNA_eEF3_GTP_4 80S_5 80S_aa-tRNA_eEF1A_GTP_5
80S_aa-tRNA_5 80S_aa-tRNA_eEF2_GTP_5 80S_tRNA_5
80S_tRNA_eEF3_GTP_5 80S_6 80S_aa-tRNA_eEF1A_GTP_6
80S_aa-tRNA_6 80S_aa-tRNA_eEF2_GTP_6 80S_tRNA_6
80S_tRNA_eEF3_GTP_6 80S_7 80S_aa-tRNA_eEF1A_GTP_7
80S_aa-tRNA_7 80S_aa-tRNA_eEF2_GTP_7 80S_tRNA_7
80S_tRNA_eEF3_GTP_7 80S_8 80S_aa-tRNA_eEF1A_GTP_8
80S_aa-tRNA_8 80S_aa-tRNA_eEF2_GTP_8 80S_tRNA_8
80S_tRNA_eEF3_GTP_8 80S_9 80S_aa-tRNA_eEF1A_GTP_9
80S_aa-tRNA_9 80S_aa-tRNA_eEF2_GTP_9 80S_tRNA_9
80S_tRNA_eEF3_GTP_9 80S_10 80S_aa-tRNA_eEF1A_GTP_10
80S_aa-tRNA_10 80S_aa-tRNA_eEF2_GTP_10 80S_tRNA_10
80S_tRNA_eEF3_GTP_10 80S_11 80S_aa-tRNA_eEF1A_GTP_11
80S_aa-tRNA_11 80S_aa-tRNA_eEF2_GTP_11 80S_tRNA_11
80S_tRNA_eEF3_GTP_11 80S_12 80S_aa-tRNA_eEF1A_GTP_12
80S_aa-tRNA_12 80S_aa-tRNA_eEF2_GTP_12 80S_tRNA_12
80S_tRNA_eEF3_GTP_12 80S_13 80S_aa-tRNA_eEF1A_GTP_13
80S_aa-tRNA_13 80S_aa-tRNA_eEF2_GTP_13 80S_tRNA_13
80S_tRNA_eEF3_GTP_13 80S_14 80S_aa-tRNA_eEF1A_GTP_14
80S_aa-tRNA_14 80S_aa-tRNA_eEF2_GTP_14 80S_tRNA_14
80S_tRNA_eEF3_GTP_14 80S_15 80S_aa-tRNA_eEF1A_GTP_15
80S_aa-tRNA_15 80S_aa-tRNA_eEF2_GTP_15 80S_tRNA_15
80S_tRNA_eEF3_GTP_15 80S_16 80S_aa-tRNA_eEF1A_GTP_16
80S_aa-tRNA_16 80S_aa-tRNA_eEF2_GTP_16 80S_tRNA_16
80S_tRNA_eEF3_GTP_16 80S_17 80S_aa-tRNA_eEF1A_GTP_17
80S_aa-tRNA_17 80S_aa-tRNA_eEF2_GTP_17 80S_tRNA_17
80S_tRNA_eEF3_GTP_17 80S_18 80S_aa-tRNA_eEF1A_GTP_18
80S_aa-tRNA_18 80S_aa-tRNA_eEF2_GTP_18 80S_tRNA_18
80S_tRNA_eEF3_GTP_18 80S_19 80S_aa-tRNA_eEF1A_GTP_19
80S_aa-tRNA_19 80S_aa-tRNA_eEF2_GTP_19 80S_tRNA_19
80S_tRNA_eEF3_GTP_19 80S_20 80S_tRNA_20
80S_tRNA_eEF3_GTP_20 eRF3_GDP eRF3_GTP
eRF1 eRF1_eRF3_GTP 80S_eRF1_eRF3_GTP
eIF4A_eIF4E_eIF4G_mRNA_Pab1    
Global parameters
mRNA_tot k22f k22b k23f
k25f k25b k26f k28f
k29f mRNA_bound mRNA_free mRNA_tot_Molar
Reactions (141)
 
 reaction1 [eIF2_GDP] + [eIF2B] ↔ [eIF2_GDP_eIF2B];   {eIF2_GDP} , {eIF2B} , {eIF2_GDP_eIF2B}
 
 reaction2 [eIF2_GDP_eIF2B] ↔ [eIF2_GTP] + [eIF2B];   {eIF2_GDP_eIF2B} , {eIF2_GTP} , {eIF2B}
 
 reaction3 [eIF2_GTP] + [Met-tRNA] ↔ [eIF2_GTP_Met-tRNA];   {eIF2_GTP} , {Met-tRNA} , {eIF2_GTP_Met-tRNA}
 
 reaction4 [eIF3] + [eIF5] ↔ [eIF3_eIF5];   {eIF3} , {eIF5} , {eIF3_eIF5}
 
 reaction5 [eIF2_GTP_Met-tRNA] + [eIF3_eIF5] ↔ [eIF3_eIF5_eIF2_GTP_Met-tRNA];   {eIF2_GTP_Met-tRNA} , {eIF3_eIF5} , {eIF3_eIF5_eIF2_GTP_Met-tRNA}
 
 reaction6 [eIF1] + [eIF3_eIF5_eIF2_GTP_Met-tRNA] ↔ [eIF1_eIF3_eIF5_eIF2_GTP_Met-tRNA];   {eIF1} , {eIF3_eIF5_eIF2_GTP_Met-tRNA} , {eIF1_eIF3_eIF5_eIF2_GTP_Met-tRNA}
 
 reaction7 [40S] + [eIF1A] → [40S_eIF1A];   {40S} , {eIF1A}
 
 reaction8 [eIF1_eIF3_eIF5_eIF2_GTP_Met-tRNA] + [40S_eIF1A] → [43S];   {eIF1_eIF3_eIF5_eIF2_GTP_Met-tRNA} , {40S_eIF1A}
 
 reaction9 [eIF4E] + [eIF4G] ↔ [eIF4E_eIF4G];   {eIF4E} , {eIF4G} , {eIF4E_eIF4G}
 
 reaction10 [mRNA_cap] + [Pab1] ↔ [mRNA_Pab1];   {mRNA_cap} , {Pab1} , {mRNA_Pab1}
 
 reaction11 [eIF4E_eIF4G] + [mRNA_Pab1] ↔ [eIF4E_eIF4G_mRNA_Pab1];   {eIF4E_eIF4G} , {mRNA_Pab1} , {eIF4E_eIF4G_mRNA_Pab1}
 
 reaction12 [eIF4A] + [eIF4E_eIF4G_mRNA_Pab1] ↔ [eIF4A_eIF4E_eIF4G_mRNA_Pab1];   {eIF4A} , {eIF4E_eIF4G_mRNA_Pab1} , {eIF4A_eIF4E_eIF4G_mRNA_Pab1}
 
 reaction13 [eIF4B] + [eIF4A_eIF4E_eIF4G_mRNA_Pab1] ↔ [eIF4E_eIF4G_mRNA_Pab1_eIF4A_eIF4B];   {eIF4B} , {eIF4A_eIF4E_eIF4G_mRNA_Pab1} , {eIF4E_eIF4G_mRNA_Pab1_eIF4A_eIF4B}
 
 reaction14 [43S] + [eIF4E_eIF4G_mRNA_Pab1_eIF4A_eIF4B] → [48S];   {43S} , {eIF4E_eIF4G_mRNA_Pab1_eIF4A_eIF4B}
 
 reaction15 [48S] + [Ded1] ↔ [48S_Ded1];   {48S} , {Ded1} , {48S_Ded1}
 
 reaction16 [eIF5B_GDP] ↔ [eIF5B_GTP];   {eIF5B_GDP} , {eIF5B_GTP}
 
 reaction17 [48S_Ded1] + [eIF5B_GTP] ↔ [48S_Ded1_eIF5B_GTP];   {48S_Ded1} , {eIF5B_GTP} , {48S_Ded1_eIF5B_GTP}
 
 reaction18 [60S] + [48S_Ded1_eIF5B_GTP] → [80S_1] + [eIF1] + [eIF1A] + [eIF2_GDP] + [eIF3] + [eIF4A] + [eIF4B] + [eIF4E] + [eIF4G] + [eIF5] + [eIF5B_GDP] + [Pab1] + [mRNA_cap] + [Ded1];   {80S_1} , {80S_aa-tRNA_eEF1A_GTP_1} , {80S_aa-tRNA_1} , {80S_aa-tRNA_eEF2_GTP_1} , {80S_2} , {80S_aa-tRNA_eEF1A_GTP_2} , {80S_aa-tRNA_2} , {80S_aa-tRNA_eEF2_GTP_2} , {80S_tRNA_2} , {80S_tRNA_eEF3_GTP_2} , {80S_3} , {80S_aa-tRNA_eEF1A_GTP_3} , {80S_aa-tRNA_3} , {80S_aa-tRNA_eEF2_GTP_3} , {80S_tRNA_3} , {80S_tRNA_eEF3_GTP_3} , {80S_4} , {80S_aa-tRNA_eEF1A_GTP_4} , {80S_aa-tRNA_4} , {80S_aa-tRNA_eEF2_GTP_4} , {80S_tRNA_4} , {80S_tRNA_eEF3_GTP_4} , {80S_5} , {80S_aa-tRNA_eEF1A_GTP_5} , {80S_aa-tRNA_5} , {80S_aa-tRNA_eEF2_GTP_5} , {80S_tRNA_5} , {80S_tRNA_eEF3_GTP_5} , {80S_6} , {80S_aa-tRNA_eEF1A_GTP_6} , {80S_aa-tRNA_6} , {80S_aa-tRNA_eEF2_GTP_6} , {80S_tRNA_6} , {80S_tRNA_eEF3_GTP_6} , {80S_7} , {80S_aa-tRNA_eEF1A_GTP_7} , {80S_aa-tRNA_7} , {80S_aa-tRNA_eEF2_GTP_7} , {80S_tRNA_7} , {80S_tRNA_eEF3_GTP_7} , {80S_8} , {80S_aa-tRNA_eEF1A_GTP_8} , {80S_aa-tRNA_8} , {80S_aa-tRNA_eEF2_GTP_8} , {80S_tRNA_8} , {80S_tRNA_eEF3_GTP_8} , {80S_9} , {80S_aa-tRNA_eEF1A_GTP_9} , {80S_aa-tRNA_9} , {80S_aa-tRNA_eEF2_GTP_9} , {80S_tRNA_9} , {80S_tRNA_eEF3_GTP_9} , {80S_10} , {80S_aa-tRNA_eEF1A_GTP_10} , {80S_aa-tRNA_10} , {80S_aa-tRNA_eEF2_GTP_10} , {80S_tRNA_10} , {80S_tRNA_eEF3_GTP_10} , {80S_11} , {80S_aa-tRNA_eEF1A_GTP_11} , {80S_aa-tRNA_11} , {80S_aa-tRNA_eEF2_GTP_11} , {80S_tRNA_11} , {80S_tRNA_eEF3_GTP_11} , {80S_12} , {80S_aa-tRNA_eEF1A_GTP_12} , {80S_aa-tRNA_12} , {80S_aa-tRNA_eEF2_GTP_12} , {80S_tRNA_12} , {80S_tRNA_eEF3_GTP_12} , {80S_13} , {80S_aa-tRNA_eEF1A_GTP_13} , {80S_aa-tRNA_13} , {80S_aa-tRNA_eEF2_GTP_13} , {80S_tRNA_13} , {80S_tRNA_eEF3_GTP_13} , {80S_14} , {80S_aa-tRNA_eEF1A_GTP_14} , {80S_aa-tRNA_14} , {80S_aa-tRNA_eEF2_GTP_14} , {80S_tRNA_14} , {80S_tRNA_eEF3_GTP_14} , {80S_15} , {80S_aa-tRNA_eEF1A_GTP_15} , {80S_aa-tRNA_15} , {80S_aa-tRNA_eEF2_GTP_15} , {80S_tRNA_15} , {80S_tRNA_eEF3_GTP_15} , {60S} , {48S_Ded1_eIF5B_GTP} , {80S_1} , {80S_aa-tRNA_eEF1A_GTP_1} , {80S_aa-tRNA_1} , {80S_aa-tRNA_eEF2_GTP_1} , {80S_2} , {80S_aa-tRNA_eEF1A_GTP_2} , {80S_aa-tRNA_2} , {80S_aa-tRNA_eEF2_GTP_2} , {80S_tRNA_2} , {80S_tRNA_eEF3_GTP_2} , {80S_3} , {80S_aa-tRNA_eEF1A_GTP_3} , {80S_aa-tRNA_3} , {80S_aa-tRNA_eEF2_GTP_3} , {80S_tRNA_3} , {80S_tRNA_eEF3_GTP_3} , {80S_4} , {80S_aa-tRNA_eEF1A_GTP_4} , {80S_aa-tRNA_4} , {80S_aa-tRNA_eEF2_GTP_4} , {80S_tRNA_4} , {80S_tRNA_eEF3_GTP_4} , {80S_5} , {80S_aa-tRNA_eEF1A_GTP_5} , {80S_aa-tRNA_5} , {80S_aa-tRNA_eEF2_GTP_5} , {80S_tRNA_5} , {80S_tRNA_eEF3_GTP_5} , {80S_6} , {80S_aa-tRNA_eEF1A_GTP_6} , {80S_aa-tRNA_6} , {80S_aa-tRNA_eEF2_GTP_6} , {80S_tRNA_6} , {80S_tRNA_eEF3_GTP_6} , {80S_7} , {80S_aa-tRNA_eEF1A_GTP_7} , {80S_aa-tRNA_7} , {80S_aa-tRNA_eEF2_GTP_7} , {80S_tRNA_7} , {80S_tRNA_eEF3_GTP_7} , {80S_8} , {80S_aa-tRNA_eEF1A_GTP_8} , {80S_aa-tRNA_8} , {80S_aa-tRNA_eEF2_GTP_8} , {80S_tRNA_8} , {80S_tRNA_eEF3_GTP_8} , {80S_9} , {80S_aa-tRNA_eEF1A_GTP_9} , {80S_aa-tRNA_9} , {80S_aa-tRNA_eEF2_GTP_9} , {80S_tRNA_9} , {80S_tRNA_eEF3_GTP_9} , {80S_10} , {80S_aa-tRNA_eEF1A_GTP_10} , {80S_aa-tRNA_10} , {80S_aa-tRNA_eEF2_GTP_10} , {80S_tRNA_10} , {80S_tRNA_eEF3_GTP_10} , {80S_11} , {80S_aa-tRNA_eEF1A_GTP_11} , {80S_aa-tRNA_11} , {80S_aa-tRNA_eEF2_GTP_11} , {80S_tRNA_11} , {80S_tRNA_eEF3_GTP_11} , {80S_12} , {80S_aa-tRNA_eEF1A_GTP_12} , {80S_aa-tRNA_12} , {80S_aa-tRNA_eEF2_GTP_12} , {80S_tRNA_12} , {80S_tRNA_eEF3_GTP_12} , {80S_13} , {80S_aa-tRNA_eEF1A_GTP_13} , {80S_aa-tRNA_13} , {80S_aa-tRNA_eEF2_GTP_13} , {80S_tRNA_13} , {80S_tRNA_eEF3_GTP_13} , {80S_14} , {80S_aa-tRNA_eEF1A_GTP_14} , {80S_aa-tRNA_14} , {80S_aa-tRNA_eEF2_GTP_14} , {80S_tRNA_14} , {80S_tRNA_eEF3_GTP_14} , {80S_15} , {80S_aa-tRNA_eEF1A_GTP_15} , {80S_aa-tRNA_15} , {80S_aa-tRNA_eEF2_GTP_15} , {80S_tRNA_15} , {80S_tRNA_eEF3_GTP_15}
 
 reaction19 [eEF1A_GDP] + [eEF1B] ↔ [eEF1A_GDP_eEF1B];   {eEF1A_GDP} , {eEF1B} , {eEF1A_GDP_eEF1B}
 
 reaction20 [eEF1A_GDP_eEF1B] ↔ [eEF1A_GTP] + [eEF1B];   {eEF1A_GDP_eEF1B} , {eEF1A_GTP} , {eEF1B}
 
 reaction21 [eEF1A_GTP] + [aa-tRNA] ↔ [aa-tRNA_eEF1A_GTP];   {eEF1A_GTP} , {aa-tRNA} , {aa-tRNA_eEF1A_GTP}
 
 reaction24 [eEF2_GDP] ↔ [eEF2_GTP];   {eEF2_GDP} , {eEF2_GTP}
 
 reaction27 [eEF3_GDP] ↔ [eEF3_GTP];   {eEF3_GDP} , {eEF3_GTP}
 
 reaction22_1 [aa-tRNA_eEF1A_GTP] + [80S_1] ↔ [80S_aa-tRNA_eEF1A_GTP_1];   {aa-tRNA_eEF1A_GTP} , {80S_1} , {80S_aa-tRNA_eEF1A_GTP_1}
 
 reaction23_1 [80S_aa-tRNA_eEF1A_GTP_1] → [80S_aa-tRNA_1] + [eEF1A_GDP];   {80S_aa-tRNA_eEF1A_GTP_1}
 
 reaction25_1 [eEF2_GTP] + [80S_aa-tRNA_1] ↔ [80S_aa-tRNA_eEF2_GTP_1];   {eEF2_GTP} , {80S_aa-tRNA_1} , {80S_aa-tRNA_eEF2_GTP_1}
 
 reaction26_1 [80S_aa-tRNA_eEF2_GTP_1] → [80S_tRNA_2] + [eEF2_GDP];   {80S_2} , {80S_aa-tRNA_eEF1A_GTP_2} , {80S_aa-tRNA_2} , {80S_aa-tRNA_eEF2_GTP_2} , {80S_tRNA_2} , {80S_tRNA_eEF3_GTP_2} , {80S_3} , {80S_aa-tRNA_eEF1A_GTP_3} , {80S_aa-tRNA_3} , {80S_aa-tRNA_eEF2_GTP_3} , {80S_tRNA_3} , {80S_tRNA_eEF3_GTP_3} , {80S_4} , {80S_aa-tRNA_eEF1A_GTP_4} , {80S_aa-tRNA_4} , {80S_aa-tRNA_eEF2_GTP_4} , {80S_tRNA_4} , {80S_tRNA_eEF3_GTP_4} , {80S_5} , {80S_aa-tRNA_eEF1A_GTP_5} , {80S_aa-tRNA_5} , {80S_aa-tRNA_eEF2_GTP_5} , {80S_tRNA_5} , {80S_tRNA_eEF3_GTP_5} , {80S_6} , {80S_aa-tRNA_eEF1A_GTP_6} , {80S_aa-tRNA_6} , {80S_aa-tRNA_eEF2_GTP_6} , {80S_tRNA_6} , {80S_tRNA_eEF3_GTP_6} , {80S_7} , {80S_aa-tRNA_eEF1A_GTP_7} , {80S_aa-tRNA_7} , {80S_aa-tRNA_eEF2_GTP_7} , {80S_tRNA_7} , {80S_tRNA_eEF3_GTP_7} , {80S_8} , {80S_aa-tRNA_eEF1A_GTP_8} , {80S_aa-tRNA_8} , {80S_aa-tRNA_eEF2_GTP_8} , {80S_tRNA_8} , {80S_tRNA_eEF3_GTP_8} , {80S_9} , {80S_aa-tRNA_eEF1A_GTP_9} , {80S_aa-tRNA_9} , {80S_aa-tRNA_eEF2_GTP_9} , {80S_tRNA_9} , {80S_tRNA_eEF3_GTP_9} , {80S_10} , {80S_aa-tRNA_eEF1A_GTP_10} , {80S_aa-tRNA_10} , {80S_aa-tRNA_eEF2_GTP_10} , {80S_tRNA_10} , {80S_tRNA_eEF3_GTP_10} , {80S_11} , {80S_aa-tRNA_eEF1A_GTP_11} , {80S_aa-tRNA_11} , {80S_aa-tRNA_eEF2_GTP_11} , {80S_tRNA_11} , {80S_tRNA_eEF3_GTP_11} , {80S_12} , {80S_aa-tRNA_eEF1A_GTP_12} , {80S_aa-tRNA_12} , {80S_aa-tRNA_eEF2_GTP_12} , {80S_tRNA_12} , {80S_tRNA_eEF3_GTP_12} , {80S_13} , {80S_aa-tRNA_eEF1A_GTP_13} , {80S_aa-tRNA_13} , {80S_aa-tRNA_eEF2_GTP_13} , {80S_tRNA_13} , {80S_tRNA_eEF3_GTP_13} , {80S_14} , {80S_aa-tRNA_eEF1A_GTP_14} , {80S_aa-tRNA_14} , {80S_aa-tRNA_eEF2_GTP_14} , {80S_tRNA_14} , {80S_tRNA_eEF3_GTP_14} , {80S_15} , {80S_aa-tRNA_eEF1A_GTP_15} , {80S_aa-tRNA_15} , {80S_aa-tRNA_eEF2_GTP_15} , {80S_tRNA_15} , {80S_tRNA_eEF3_GTP_15} , {80S_16} , {80S_aa-tRNA_eEF1A_GTP_16} , {80S_aa-tRNA_16} , {80S_aa-tRNA_eEF2_GTP_16} , {80S_tRNA_16} , {80S_tRNA_eEF3_GTP_16} , {80S_aa-tRNA_eEF2_GTP_1} , {80S_2} , {80S_aa-tRNA_eEF1A_GTP_2} , {80S_aa-tRNA_2} , {80S_aa-tRNA_eEF2_GTP_2} , {80S_tRNA_2} , {80S_tRNA_eEF3_GTP_2} , {80S_3} , {80S_aa-tRNA_eEF1A_GTP_3} , {80S_aa-tRNA_3} , {80S_aa-tRNA_eEF2_GTP_3} , {80S_tRNA_3} , {80S_tRNA_eEF3_GTP_3} , {80S_4} , {80S_aa-tRNA_eEF1A_GTP_4} , {80S_aa-tRNA_4} , {80S_aa-tRNA_eEF2_GTP_4} , {80S_tRNA_4} , {80S_tRNA_eEF3_GTP_4} , {80S_5} , {80S_aa-tRNA_eEF1A_GTP_5} , {80S_aa-tRNA_5} , {80S_aa-tRNA_eEF2_GTP_5} , {80S_tRNA_5} , {80S_tRNA_eEF3_GTP_5} , {80S_6} , {80S_aa-tRNA_eEF1A_GTP_6} , {80S_aa-tRNA_6} , {80S_aa-tRNA_eEF2_GTP_6} , {80S_tRNA_6} , {80S_tRNA_eEF3_GTP_6} , {80S_7} , {80S_aa-tRNA_eEF1A_GTP_7} , {80S_aa-tRNA_7} , {80S_aa-tRNA_eEF2_GTP_7} , {80S_tRNA_7} , {80S_tRNA_eEF3_GTP_7} , {80S_8} , {80S_aa-tRNA_eEF1A_GTP_8} , {80S_aa-tRNA_8} , {80S_aa-tRNA_eEF2_GTP_8} , {80S_tRNA_8} , {80S_tRNA_eEF3_GTP_8} , {80S_9} , {80S_aa-tRNA_eEF1A_GTP_9} , {80S_aa-tRNA_9} , {80S_aa-tRNA_eEF2_GTP_9} , {80S_tRNA_9} , {80S_tRNA_eEF3_GTP_9} , {80S_10} , {80S_aa-tRNA_eEF1A_GTP_10} , {80S_aa-tRNA_10} , {80S_aa-tRNA_eEF2_GTP_10} , {80S_tRNA_10} , {80S_tRNA_eEF3_GTP_10} , {80S_11} , {80S_aa-tRNA_eEF1A_GTP_11} , {80S_aa-tRNA_11} , {80S_aa-tRNA_eEF2_GTP_11} , {80S_tRNA_11} , {80S_tRNA_eEF3_GTP_11} , {80S_12} , {80S_aa-tRNA_eEF1A_GTP_12} , {80S_aa-tRNA_12} , {80S_aa-tRNA_eEF2_GTP_12} , {80S_tRNA_12} , {80S_tRNA_eEF3_GTP_12} , {80S_13} , {80S_aa-tRNA_eEF1A_GTP_13} , {80S_aa-tRNA_13} , {80S_aa-tRNA_eEF2_GTP_13} , {80S_tRNA_13} , {80S_tRNA_eEF3_GTP_13} , {80S_14} , {80S_aa-tRNA_eEF1A_GTP_14} , {80S_aa-tRNA_14} , {80S_aa-tRNA_eEF2_GTP_14} , {80S_tRNA_14} , {80S_tRNA_eEF3_GTP_14} , {80S_15} , {80S_aa-tRNA_eEF1A_GTP_15} , {80S_aa-tRNA_15} , {80S_aa-tRNA_eEF2_GTP_15} , {80S_tRNA_15} , {80S_tRNA_eEF3_GTP_15} , {80S_16} , {80S_aa-tRNA_eEF1A_GTP_16} , {80S_aa-tRNA_16} , {80S_aa-tRNA_eEF2_GTP_16} , {80S_tRNA_16} , {80S_tRNA_eEF3_GTP_16}
 
 reaction22_2 [aa-tRNA_eEF1A_GTP] + [80S_2] ↔ [80S_aa-tRNA_eEF1A_GTP_2];   {aa-tRNA_eEF1A_GTP} , {80S_2} , {80S_aa-tRNA_eEF1A_GTP_2}
 
 reaction23_2 [80S_aa-tRNA_eEF1A_GTP_2] → [80S_aa-tRNA_2] + [eEF1A_GDP];   {80S_aa-tRNA_eEF1A_GTP_2}
 
 reaction25_2 [eEF2_GTP] + [80S_aa-tRNA_2] ↔ [80S_aa-tRNA_eEF2_GTP_2];   {eEF2_GTP} , {80S_aa-tRNA_2} , {80S_aa-tRNA_eEF2_GTP_2}
 
 reaction26_2 [80S_aa-tRNA_eEF2_GTP_2] → [80S_tRNA_3] + [eEF2_GDP];   {80S_3} , {80S_aa-tRNA_eEF1A_GTP_3} , {80S_aa-tRNA_3} , {80S_aa-tRNA_eEF2_GTP_3} , {80S_tRNA_3} , {80S_tRNA_eEF3_GTP_3} , {80S_4} , {80S_aa-tRNA_eEF1A_GTP_4} , {80S_aa-tRNA_4} , {80S_aa-tRNA_eEF2_GTP_4} , {80S_tRNA_4} , {80S_tRNA_eEF3_GTP_4} , {80S_5} , {80S_aa-tRNA_eEF1A_GTP_5} , {80S_aa-tRNA_5} , {80S_aa-tRNA_eEF2_GTP_5} , {80S_tRNA_5} , {80S_tRNA_eEF3_GTP_5} , {80S_6} , {80S_aa-tRNA_eEF1A_GTP_6} , {80S_aa-tRNA_6} , {80S_aa-tRNA_eEF2_GTP_6} , {80S_tRNA_6} , {80S_tRNA_eEF3_GTP_6} , {80S_7} , {80S_aa-tRNA_eEF1A_GTP_7} , {80S_aa-tRNA_7} , {80S_aa-tRNA_eEF2_GTP_7} , {80S_tRNA_7} , {80S_tRNA_eEF3_GTP_7} , {80S_8} , {80S_aa-tRNA_eEF1A_GTP_8} , {80S_aa-tRNA_8} , {80S_aa-tRNA_eEF2_GTP_8} , {80S_tRNA_8} , {80S_tRNA_eEF3_GTP_8} , {80S_9} , {80S_aa-tRNA_eEF1A_GTP_9} , {80S_aa-tRNA_9} , {80S_aa-tRNA_eEF2_GTP_9} , {80S_tRNA_9} , {80S_tRNA_eEF3_GTP_9} , {80S_10} , {80S_aa-tRNA_eEF1A_GTP_10} , {80S_aa-tRNA_10} , {80S_aa-tRNA_eEF2_GTP_10} , {80S_tRNA_10} , {80S_tRNA_eEF3_GTP_10} , {80S_11} , {80S_aa-tRNA_eEF1A_GTP_11} , {80S_aa-tRNA_11} , {80S_aa-tRNA_eEF2_GTP_11} , {80S_tRNA_11} , {80S_tRNA_eEF3_GTP_11} , {80S_12} , {80S_aa-tRNA_eEF1A_GTP_12} , {80S_aa-tRNA_12} , {80S_aa-tRNA_eEF2_GTP_12} , {80S_tRNA_12} , {80S_tRNA_eEF3_GTP_12} , {80S_13} , {80S_aa-tRNA_eEF1A_GTP_13} , {80S_aa-tRNA_13} , {80S_aa-tRNA_eEF2_GTP_13} , {80S_tRNA_13} , {80S_tRNA_eEF3_GTP_13} , {80S_14} , {80S_aa-tRNA_eEF1A_GTP_14} , {80S_aa-tRNA_14} , {80S_aa-tRNA_eEF2_GTP_14} , {80S_tRNA_14} , {80S_tRNA_eEF3_GTP_14} , {80S_15} , {80S_aa-tRNA_eEF1A_GTP_15} , {80S_aa-tRNA_15} , {80S_aa-tRNA_eEF2_GTP_15} , {80S_tRNA_15} , {80S_tRNA_eEF3_GTP_15} , {80S_16} , {80S_aa-tRNA_eEF1A_GTP_16} , {80S_aa-tRNA_16} , {80S_aa-tRNA_eEF2_GTP_16} , {80S_tRNA_16} , {80S_tRNA_eEF3_GTP_16} , {80S_17} , {80S_aa-tRNA_eEF1A_GTP_17} , {80S_aa-tRNA_17} , {80S_aa-tRNA_eEF2_GTP_17} , {80S_tRNA_17} , {80S_tRNA_eEF3_GTP_17} , {80S_aa-tRNA_eEF2_GTP_2} , {80S_3} , {80S_aa-tRNA_eEF1A_GTP_3} , {80S_aa-tRNA_3} , {80S_aa-tRNA_eEF2_GTP_3} , {80S_tRNA_3} , {80S_tRNA_eEF3_GTP_3} , {80S_4} , {80S_aa-tRNA_eEF1A_GTP_4} , {80S_aa-tRNA_4} , {80S_aa-tRNA_eEF2_GTP_4} , {80S_tRNA_4} , {80S_tRNA_eEF3_GTP_4} , {80S_5} , {80S_aa-tRNA_eEF1A_GTP_5} , {80S_aa-tRNA_5} , {80S_aa-tRNA_eEF2_GTP_5} , {80S_tRNA_5} , {80S_tRNA_eEF3_GTP_5} , {80S_6} , {80S_aa-tRNA_eEF1A_GTP_6} , {80S_aa-tRNA_6} , {80S_aa-tRNA_eEF2_GTP_6} , {80S_tRNA_6} , {80S_tRNA_eEF3_GTP_6} , {80S_7} , {80S_aa-tRNA_eEF1A_GTP_7} , {80S_aa-tRNA_7} , {80S_aa-tRNA_eEF2_GTP_7} , {80S_tRNA_7} , {80S_tRNA_eEF3_GTP_7} , {80S_8} , {80S_aa-tRNA_eEF1A_GTP_8} , {80S_aa-tRNA_8} , {80S_aa-tRNA_eEF2_GTP_8} , {80S_tRNA_8} , {80S_tRNA_eEF3_GTP_8} , {80S_9} , {80S_aa-tRNA_eEF1A_GTP_9} , {80S_aa-tRNA_9} , {80S_aa-tRNA_eEF2_GTP_9} , {80S_tRNA_9} , {80S_tRNA_eEF3_GTP_9} , {80S_10} , {80S_aa-tRNA_eEF1A_GTP_10} , {80S_aa-tRNA_10} , {80S_aa-tRNA_eEF2_GTP_10} , {80S_tRNA_10} , {80S_tRNA_eEF3_GTP_10} , {80S_11} , {80S_aa-tRNA_eEF1A_GTP_11} , {80S_aa-tRNA_11} , {80S_aa-tRNA_eEF2_GTP_11} , {80S_tRNA_11} , {80S_tRNA_eEF3_GTP_11} , {80S_12} , {80S_aa-tRNA_eEF1A_GTP_12} , {80S_aa-tRNA_12} , {80S_aa-tRNA_eEF2_GTP_12} , {80S_tRNA_12} , {80S_tRNA_eEF3_GTP_12} , {80S_13} , {80S_aa-tRNA_eEF1A_GTP_13} , {80S_aa-tRNA_13} , {80S_aa-tRNA_eEF2_GTP_13} , {80S_tRNA_13} , {80S_tRNA_eEF3_GTP_13} , {80S_14} , {80S_aa-tRNA_eEF1A_GTP_14} , {80S_aa-tRNA_14} , {80S_aa-tRNA_eEF2_GTP_14} , {80S_tRNA_14} , {80S_tRNA_eEF3_GTP_14} , {80S_15} , {80S_aa-tRNA_eEF1A_GTP_15} , {80S_aa-tRNA_15} , {80S_aa-tRNA_eEF2_GTP_15} , {80S_tRNA_15} , {80S_tRNA_eEF3_GTP_15} , {80S_16} , {80S_aa-tRNA_eEF1A_GTP_16} , {80S_aa-tRNA_16} , {80S_aa-tRNA_eEF2_GTP_16} , {80S_tRNA_16} , {80S_tRNA_eEF3_GTP_16} , {80S_17} , {80S_aa-tRNA_eEF1A_GTP_17} , {80S_aa-tRNA_17} , {80S_aa-tRNA_eEF2_GTP_17} , {80S_tRNA_17} , {80S_tRNA_eEF3_GTP_17}
 
 reaction28_2 [80S_tRNA_2] + [eEF3_GTP] → [80S_tRNA_eEF3_GTP_2];   {80S_tRNA_2} , {eEF3_GTP}
 
 reaction29_2 [80S_tRNA_eEF3_GTP_2] → [80S_2] + [eEF3_GDP] + [tRNA];   {80S_tRNA_eEF3_GTP_2}
 
 reaction22_3 [aa-tRNA_eEF1A_GTP] + [80S_3] ↔ [80S_aa-tRNA_eEF1A_GTP_3];   {aa-tRNA_eEF1A_GTP} , {80S_3} , {80S_aa-tRNA_eEF1A_GTP_3}
 
 reaction23_3 [80S_aa-tRNA_eEF1A_GTP_3] → [80S_aa-tRNA_3] + [eEF1A_GDP];   {80S_aa-tRNA_eEF1A_GTP_3}
 
 reaction25_3 [eEF2_GTP] + [80S_aa-tRNA_3] ↔ [80S_aa-tRNA_eEF2_GTP_3];   {eEF2_GTP} , {80S_aa-tRNA_3} , {80S_aa-tRNA_eEF2_GTP_3}
 
 reaction26_3 [80S_aa-tRNA_eEF2_GTP_3] → [80S_tRNA_4] + [eEF2_GDP];   {80S_4} , {80S_aa-tRNA_eEF1A_GTP_4} , {80S_aa-tRNA_4} , {80S_aa-tRNA_eEF2_GTP_4} , {80S_tRNA_4} , {80S_tRNA_eEF3_GTP_4} , {80S_5} , {80S_aa-tRNA_eEF1A_GTP_5} , {80S_aa-tRNA_5} , {80S_aa-tRNA_eEF2_GTP_5} , {80S_tRNA_5} , {80S_tRNA_eEF3_GTP_5} , {80S_6} , {80S_aa-tRNA_eEF1A_GTP_6} , {80S_aa-tRNA_6} , {80S_aa-tRNA_eEF2_GTP_6} , {80S_tRNA_6} , {80S_tRNA_eEF3_GTP_6} , {80S_7} , {80S_aa-tRNA_eEF1A_GTP_7} , {80S_aa-tRNA_7} , {80S_aa-tRNA_eEF2_GTP_7} , {80S_tRNA_7} , {80S_tRNA_eEF3_GTP_7} , {80S_8} , {80S_aa-tRNA_eEF1A_GTP_8} , {80S_aa-tRNA_8} , {80S_aa-tRNA_eEF2_GTP_8} , {80S_tRNA_8} , {80S_tRNA_eEF3_GTP_8} , {80S_9} , {80S_aa-tRNA_eEF1A_GTP_9} , {80S_aa-tRNA_9} , {80S_aa-tRNA_eEF2_GTP_9} , {80S_tRNA_9} , {80S_tRNA_eEF3_GTP_9} , {80S_10} , {80S_aa-tRNA_eEF1A_GTP_10} , {80S_aa-tRNA_10} , {80S_aa-tRNA_eEF2_GTP_10} , {80S_tRNA_10} , {80S_tRNA_eEF3_GTP_10} , {80S_11} , {80S_aa-tRNA_eEF1A_GTP_11} , {80S_aa-tRNA_11} , {80S_aa-tRNA_eEF2_GTP_11} , {80S_tRNA_11} , {80S_tRNA_eEF3_GTP_11} , {80S_12} , {80S_aa-tRNA_eEF1A_GTP_12} , {80S_aa-tRNA_12} , {80S_aa-tRNA_eEF2_GTP_12} , {80S_tRNA_12} , {80S_tRNA_eEF3_GTP_12} , {80S_13} , {80S_aa-tRNA_eEF1A_GTP_13} , {80S_aa-tRNA_13} , {80S_aa-tRNA_eEF2_GTP_13} , {80S_tRNA_13} , {80S_tRNA_eEF3_GTP_13} , {80S_14} , {80S_aa-tRNA_eEF1A_GTP_14} , {80S_aa-tRNA_14} , {80S_aa-tRNA_eEF2_GTP_14} , {80S_tRNA_14} , {80S_tRNA_eEF3_GTP_14} , {80S_15} , {80S_aa-tRNA_eEF1A_GTP_15} , {80S_aa-tRNA_15} , {80S_aa-tRNA_eEF2_GTP_15} , {80S_tRNA_15} , {80S_tRNA_eEF3_GTP_15} , {80S_16} , {80S_aa-tRNA_eEF1A_GTP_16} , {80S_aa-tRNA_16} , {80S_aa-tRNA_eEF2_GTP_16} , {80S_tRNA_16} , {80S_tRNA_eEF3_GTP_16} , {80S_17} , {80S_aa-tRNA_eEF1A_GTP_17} , {80S_aa-tRNA_17} , {80S_aa-tRNA_eEF2_GTP_17} , {80S_tRNA_17} , {80S_tRNA_eEF3_GTP_17} , {80S_18} , {80S_aa-tRNA_eEF1A_GTP_18} , {80S_aa-tRNA_18} , {80S_aa-tRNA_eEF2_GTP_18} , {80S_tRNA_18} , {80S_tRNA_eEF3_GTP_18} , {80S_aa-tRNA_eEF2_GTP_3} , {80S_4} , {80S_aa-tRNA_eEF1A_GTP_4} , {80S_aa-tRNA_4} , {80S_aa-tRNA_eEF2_GTP_4} , {80S_tRNA_4} , {80S_tRNA_eEF3_GTP_4} , {80S_5} , {80S_aa-tRNA_eEF1A_GTP_5} , {80S_aa-tRNA_5} , {80S_aa-tRNA_eEF2_GTP_5} , {80S_tRNA_5} , {80S_tRNA_eEF3_GTP_5} , {80S_6} , {80S_aa-tRNA_eEF1A_GTP_6} , {80S_aa-tRNA_6} , {80S_aa-tRNA_eEF2_GTP_6} , {80S_tRNA_6} , {80S_tRNA_eEF3_GTP_6} , {80S_7} , {80S_aa-tRNA_eEF1A_GTP_7} , {80S_aa-tRNA_7} , {80S_aa-tRNA_eEF2_GTP_7} , {80S_tRNA_7} , {80S_tRNA_eEF3_GTP_7} , {80S_8} , {80S_aa-tRNA_eEF1A_GTP_8} , {80S_aa-tRNA_8} , {80S_aa-tRNA_eEF2_GTP_8} , {80S_tRNA_8} , {80S_tRNA_eEF3_GTP_8} , {80S_9} , {80S_aa-tRNA_eEF1A_GTP_9} , {80S_aa-tRNA_9} , {80S_aa-tRNA_eEF2_GTP_9} , {80S_tRNA_9} , {80S_tRNA_eEF3_GTP_9} , {80S_10} , {80S_aa-tRNA_eEF1A_GTP_10} , {80S_aa-tRNA_10} , {80S_aa-tRNA_eEF2_GTP_10} , {80S_tRNA_10} , {80S_tRNA_eEF3_GTP_10} , {80S_11} , {80S_aa-tRNA_eEF1A_GTP_11} , {80S_aa-tRNA_11} , {80S_aa-tRNA_eEF2_GTP_11} , {80S_tRNA_11} , {80S_tRNA_eEF3_GTP_11} , {80S_12} , {80S_aa-tRNA_eEF1A_GTP_12} , {80S_aa-tRNA_12} , {80S_aa-tRNA_eEF2_GTP_12} , {80S_tRNA_12} , {80S_tRNA_eEF3_GTP_12} , {80S_13} , {80S_aa-tRNA_eEF1A_GTP_13} , {80S_aa-tRNA_13} , {80S_aa-tRNA_eEF2_GTP_13} , {80S_tRNA_13} , {80S_tRNA_eEF3_GTP_13} , {80S_14} , {80S_aa-tRNA_eEF1A_GTP_14} , {80S_aa-tRNA_14} , {80S_aa-tRNA_eEF2_GTP_14} , {80S_tRNA_14} , {80S_tRNA_eEF3_GTP_14} , {80S_15} , {80S_aa-tRNA_eEF1A_GTP_15} , {80S_aa-tRNA_15} , {80S_aa-tRNA_eEF2_GTP_15} , {80S_tRNA_15} , {80S_tRNA_eEF3_GTP_15} , {80S_16} , {80S_aa-tRNA_eEF1A_GTP_16} , {80S_aa-tRNA_16} , {80S_aa-tRNA_eEF2_GTP_16} , {80S_tRNA_16} , {80S_tRNA_eEF3_GTP_16} , {80S_17} , {80S_aa-tRNA_eEF1A_GTP_17} , {80S_aa-tRNA_17} , {80S_aa-tRNA_eEF2_GTP_17} , {80S_tRNA_17} , {80S_tRNA_eEF3_GTP_17} , {80S_18} , {80S_aa-tRNA_eEF1A_GTP_18} , {80S_aa-tRNA_18} , {80S_aa-tRNA_eEF2_GTP_18} , {80S_tRNA_18} , {80S_tRNA_eEF3_GTP_18}
 
 reaction28_3 [80S_tRNA_3] + [eEF3_GTP] → [80S_tRNA_eEF3_GTP_3];   {80S_tRNA_3} , {eEF3_GTP}
 
 reaction29_3 [80S_tRNA_eEF3_GTP_3] → [80S_3] + [eEF3_GDP] + [tRNA];   {80S_tRNA_eEF3_GTP_3}
 
 reaction22_4 [aa-tRNA_eEF1A_GTP] + [80S_4] ↔ [80S_aa-tRNA_eEF1A_GTP_4];   {aa-tRNA_eEF1A_GTP} , {80S_4} , {80S_aa-tRNA_eEF1A_GTP_4}
 
 reaction23_4 [80S_aa-tRNA_eEF1A_GTP_4] → [80S_aa-tRNA_4] + [eEF1A_GDP];   {80S_aa-tRNA_eEF1A_GTP_4}
 
 reaction25_4 [eEF2_GTP] + [80S_aa-tRNA_4] ↔ [80S_aa-tRNA_eEF2_GTP_4];   {eEF2_GTP} , {80S_aa-tRNA_4} , {80S_aa-tRNA_eEF2_GTP_4}
 
 reaction26_4 [80S_aa-tRNA_eEF2_GTP_4] → [80S_tRNA_5] + [eEF2_GDP];   {80S_5} , {80S_aa-tRNA_eEF1A_GTP_5} , {80S_aa-tRNA_5} , {80S_aa-tRNA_eEF2_GTP_5} , {80S_tRNA_5} , {80S_tRNA_eEF3_GTP_5} , {80S_6} , {80S_aa-tRNA_eEF1A_GTP_6} , {80S_aa-tRNA_6} , {80S_aa-tRNA_eEF2_GTP_6} , {80S_tRNA_6} , {80S_tRNA_eEF3_GTP_6} , {80S_7} , {80S_aa-tRNA_eEF1A_GTP_7} , {80S_aa-tRNA_7} , {80S_aa-tRNA_eEF2_GTP_7} , {80S_tRNA_7} , {80S_tRNA_eEF3_GTP_7} , {80S_8} , {80S_aa-tRNA_eEF1A_GTP_8} , {80S_aa-tRNA_8} , {80S_aa-tRNA_eEF2_GTP_8} , {80S_tRNA_8} , {80S_tRNA_eEF3_GTP_8} , {80S_9} , {80S_aa-tRNA_eEF1A_GTP_9} , {80S_aa-tRNA_9} , {80S_aa-tRNA_eEF2_GTP_9} , {80S_tRNA_9} , {80S_tRNA_eEF3_GTP_9} , {80S_10} , {80S_aa-tRNA_eEF1A_GTP_10} , {80S_aa-tRNA_10} , {80S_aa-tRNA_eEF2_GTP_10} , {80S_tRNA_10} , {80S_tRNA_eEF3_GTP_10} , {80S_11} , {80S_aa-tRNA_eEF1A_GTP_11} , {80S_aa-tRNA_11} , {80S_aa-tRNA_eEF2_GTP_11} , {80S_tRNA_11} , {80S_tRNA_eEF3_GTP_11} , {80S_12} , {80S_aa-tRNA_eEF1A_GTP_12} , {80S_aa-tRNA_12} , {80S_aa-tRNA_eEF2_GTP_12} , {80S_tRNA_12} , {80S_tRNA_eEF3_GTP_12} , {80S_13} , {80S_aa-tRNA_eEF1A_GTP_13} , {80S_aa-tRNA_13} , {80S_aa-tRNA_eEF2_GTP_13} , {80S_tRNA_13} , {80S_tRNA_eEF3_GTP_13} , {80S_14} , {80S_aa-tRNA_eEF1A_GTP_14} , {80S_aa-tRNA_14} , {80S_aa-tRNA_eEF2_GTP_14} , {80S_tRNA_14} , {80S_tRNA_eEF3_GTP_14} , {80S_15} , {80S_aa-tRNA_eEF1A_GTP_15} , {80S_aa-tRNA_15} , {80S_aa-tRNA_eEF2_GTP_15} , {80S_tRNA_15} , {80S_tRNA_eEF3_GTP_15} , {80S_16} , {80S_aa-tRNA_eEF1A_GTP_16} , {80S_aa-tRNA_16} , {80S_aa-tRNA_eEF2_GTP_16} , {80S_tRNA_16} , {80S_tRNA_eEF3_GTP_16} , {80S_17} , {80S_aa-tRNA_eEF1A_GTP_17} , {80S_aa-tRNA_17} , {80S_aa-tRNA_eEF2_GTP_17} , {80S_tRNA_17} , {80S_tRNA_eEF3_GTP_17} , {80S_18} , {80S_aa-tRNA_eEF1A_GTP_18} , {80S_aa-tRNA_18} , {80S_aa-tRNA_eEF2_GTP_18} , {80S_tRNA_18} , {80S_tRNA_eEF3_GTP_18} , {80S_19} , {80S_aa-tRNA_eEF1A_GTP_19} , {80S_aa-tRNA_19} , {80S_aa-tRNA_eEF2_GTP_19} , {80S_tRNA_19} , {80S_tRNA_eEF3_GTP_19} , {80S_aa-tRNA_eEF2_GTP_4} , {80S_5} , {80S_aa-tRNA_eEF1A_GTP_5} , {80S_aa-tRNA_5} , {80S_aa-tRNA_eEF2_GTP_5} , {80S_tRNA_5} , {80S_tRNA_eEF3_GTP_5} , {80S_6} , {80S_aa-tRNA_eEF1A_GTP_6} , {80S_aa-tRNA_6} , {80S_aa-tRNA_eEF2_GTP_6} , {80S_tRNA_6} , {80S_tRNA_eEF3_GTP_6} , {80S_7} , {80S_aa-tRNA_eEF1A_GTP_7} , {80S_aa-tRNA_7} , {80S_aa-tRNA_eEF2_GTP_7} , {80S_tRNA_7} , {80S_tRNA_eEF3_GTP_7} , {80S_8} , {80S_aa-tRNA_eEF1A_GTP_8} , {80S_aa-tRNA_8} , {80S_aa-tRNA_eEF2_GTP_8} , {80S_tRNA_8} , {80S_tRNA_eEF3_GTP_8} , {80S_9} , {80S_aa-tRNA_eEF1A_GTP_9} , {80S_aa-tRNA_9} , {80S_aa-tRNA_eEF2_GTP_9} , {80S_tRNA_9} , {80S_tRNA_eEF3_GTP_9} , {80S_10} , {80S_aa-tRNA_eEF1A_GTP_10} , {80S_aa-tRNA_10} , {80S_aa-tRNA_eEF2_GTP_10} , {80S_tRNA_10} , {80S_tRNA_eEF3_GTP_10} , {80S_11} , {80S_aa-tRNA_eEF1A_GTP_11} , {80S_aa-tRNA_11} , {80S_aa-tRNA_eEF2_GTP_11} , {80S_tRNA_11} , {80S_tRNA_eEF3_GTP_11} , {80S_12} , {80S_aa-tRNA_eEF1A_GTP_12} , {80S_aa-tRNA_12} , {80S_aa-tRNA_eEF2_GTP_12} , {80S_tRNA_12} , {80S_tRNA_eEF3_GTP_12} , {80S_13} , {80S_aa-tRNA_eEF1A_GTP_13} , {80S_aa-tRNA_13} , {80S_aa-tRNA_eEF2_GTP_13} , {80S_tRNA_13} , {80S_tRNA_eEF3_GTP_13} , {80S_14} , {80S_aa-tRNA_eEF1A_GTP_14} , {80S_aa-tRNA_14} , {80S_aa-tRNA_eEF2_GTP_14} , {80S_tRNA_14} , {80S_tRNA_eEF3_GTP_14} , {80S_15} , {80S_aa-tRNA_eEF1A_GTP_15} , {80S_aa-tRNA_15} , {80S_aa-tRNA_eEF2_GTP_15} , {80S_tRNA_15} , {80S_tRNA_eEF3_GTP_15} , {80S_16} , {80S_aa-tRNA_eEF1A_GTP_16} , {80S_aa-tRNA_16} , {80S_aa-tRNA_eEF2_GTP_16} , {80S_tRNA_16} , {80S_tRNA_eEF3_GTP_16} , {80S_17} , {80S_aa-tRNA_eEF1A_GTP_17} , {80S_aa-tRNA_17} , {80S_aa-tRNA_eEF2_GTP_17} , {80S_tRNA_17} , {80S_tRNA_eEF3_GTP_17} , {80S_18} , {80S_aa-tRNA_eEF1A_GTP_18} , {80S_aa-tRNA_18} , {80S_aa-tRNA_eEF2_GTP_18} , {80S_tRNA_18} , {80S_tRNA_eEF3_GTP_18} , {80S_19} , {80S_aa-tRNA_eEF1A_GTP_19} , {80S_aa-tRNA_19} , {80S_aa-tRNA_eEF2_GTP_19} , {80S_tRNA_19} , {80S_tRNA_eEF3_GTP_19}
 
 reaction28_4 [80S_tRNA_4] + [eEF3_GTP] → [80S_tRNA_eEF3_GTP_4];   {80S_tRNA_4} , {eEF3_GTP}
 
 reaction29_4 [80S_tRNA_eEF3_GTP_4] → [80S_4] + [eEF3_GDP] + [tRNA];   {80S_tRNA_eEF3_GTP_4}
 
 reaction22_5 [aa-tRNA_eEF1A_GTP] + [80S_5] ↔ [80S_aa-tRNA_eEF1A_GTP_5];   {aa-tRNA_eEF1A_GTP} , {80S_5} , {80S_aa-tRNA_eEF1A_GTP_5}
 
 reaction23_5 [80S_aa-tRNA_eEF1A_GTP_5] → [80S_aa-tRNA_5] + [eEF1A_GDP];   {80S_aa-tRNA_eEF1A_GTP_5}
 
 reaction25_5 [eEF2_GTP] + [80S_aa-tRNA_5] ↔ [80S_aa-tRNA_eEF2_GTP_5];   {eEF2_GTP} , {80S_aa-tRNA_5} , {80S_aa-tRNA_eEF2_GTP_5}
 
 reaction26_5 [80S_aa-tRNA_eEF2_GTP_5] → [80S_tRNA_6] + [eEF2_GDP];   {80S_6} , {80S_aa-tRNA_eEF1A_GTP_6} , {80S_aa-tRNA_6} , {80S_aa-tRNA_eEF2_GTP_6} , {80S_tRNA_6} , {80S_tRNA_eEF3_GTP_6} , {80S_7} , {80S_aa-tRNA_eEF1A_GTP_7} , {80S_aa-tRNA_7} , {80S_aa-tRNA_eEF2_GTP_7} , {80S_tRNA_7} , {80S_tRNA_eEF3_GTP_7} , {80S_8} , {80S_aa-tRNA_eEF1A_GTP_8} , {80S_aa-tRNA_8} , {80S_aa-tRNA_eEF2_GTP_8} , {80S_tRNA_8} , {80S_tRNA_eEF3_GTP_8} , {80S_9} , {80S_aa-tRNA_eEF1A_GTP_9} , {80S_aa-tRNA_9} , {80S_aa-tRNA_eEF2_GTP_9} , {80S_tRNA_9} , {80S_tRNA_eEF3_GTP_9} , {80S_10} , {80S_aa-tRNA_eEF1A_GTP_10} , {80S_aa-tRNA_10} , {80S_aa-tRNA_eEF2_GTP_10} , {80S_tRNA_10} , {80S_tRNA_eEF3_GTP_10} , {80S_11} , {80S_aa-tRNA_eEF1A_GTP_11} , {80S_aa-tRNA_11} , {80S_aa-tRNA_eEF2_GTP_11} , {80S_tRNA_11} , {80S_tRNA_eEF3_GTP_11} , {80S_12} , {80S_aa-tRNA_eEF1A_GTP_12} , {80S_aa-tRNA_12} , {80S_aa-tRNA_eEF2_GTP_12} , {80S_tRNA_12} , {80S_tRNA_eEF3_GTP_12} , {80S_13} , {80S_aa-tRNA_eEF1A_GTP_13} , {80S_aa-tRNA_13} , {80S_aa-tRNA_eEF2_GTP_13} , {80S_tRNA_13} , {80S_tRNA_eEF3_GTP_13} , {80S_14} , {80S_aa-tRNA_eEF1A_GTP_14} , {80S_aa-tRNA_14} , {80S_aa-tRNA_eEF2_GTP_14} , {80S_tRNA_14} , {80S_tRNA_eEF3_GTP_14} , {80S_15} , {80S_aa-tRNA_eEF1A_GTP_15} , {80S_aa-tRNA_15} , {80S_aa-tRNA_eEF2_GTP_15} , {80S_tRNA_15} , {80S_tRNA_eEF3_GTP_15} , {80S_16} , {80S_aa-tRNA_eEF1A_GTP_16} , {80S_aa-tRNA_16} , {80S_aa-tRNA_eEF2_GTP_16} , {80S_tRNA_16} , {80S_tRNA_eEF3_GTP_16} , {80S_17} , {80S_aa-tRNA_eEF1A_GTP_17} , {80S_aa-tRNA_17} , {80S_aa-tRNA_eEF2_GTP_17} , {80S_tRNA_17} , {80S_tRNA_eEF3_GTP_17} , {80S_18} , {80S_aa-tRNA_eEF1A_GTP_18} , {80S_aa-tRNA_18} , {80S_aa-tRNA_eEF2_GTP_18} , {80S_tRNA_18} , {80S_tRNA_eEF3_GTP_18} , {80S_19} , {80S_aa-tRNA_eEF1A_GTP_19} , {80S_aa-tRNA_19} , {80S_aa-tRNA_eEF2_GTP_19} , {80S_tRNA_19} , {80S_tRNA_eEF3_GTP_19} , {80S_20} , {80S_tRNA_20} , {80S_tRNA_eEF3_GTP_20} , {80S_aa-tRNA_eEF2_GTP_5} , {80S_6} , {80S_aa-tRNA_eEF1A_GTP_6} , {80S_aa-tRNA_6} , {80S_aa-tRNA_eEF2_GTP_6} , {80S_tRNA_6} , {80S_tRNA_eEF3_GTP_6} , {80S_7} , {80S_aa-tRNA_eEF1A_GTP_7} , {80S_aa-tRNA_7} , {80S_aa-tRNA_eEF2_GTP_7} , {80S_tRNA_7} , {80S_tRNA_eEF3_GTP_7} , {80S_8} , {80S_aa-tRNA_eEF1A_GTP_8} , {80S_aa-tRNA_8} , {80S_aa-tRNA_eEF2_GTP_8} , {80S_tRNA_8} , {80S_tRNA_eEF3_GTP_8} , {80S_9} , {80S_aa-tRNA_eEF1A_GTP_9} , {80S_aa-tRNA_9} , {80S_aa-tRNA_eEF2_GTP_9} , {80S_tRNA_9} , {80S_tRNA_eEF3_GTP_9} , {80S_10} , {80S_aa-tRNA_eEF1A_GTP_10} , {80S_aa-tRNA_10} , {80S_aa-tRNA_eEF2_GTP_10} , {80S_tRNA_10} , {80S_tRNA_eEF3_GTP_10} , {80S_11} , {80S_aa-tRNA_eEF1A_GTP_11} , {80S_aa-tRNA_11} , {80S_aa-tRNA_eEF2_GTP_11} , {80S_tRNA_11} , {80S_tRNA_eEF3_GTP_11} , {80S_12} , {80S_aa-tRNA_eEF1A_GTP_12} , {80S_aa-tRNA_12} , {80S_aa-tRNA_eEF2_GTP_12} , {80S_tRNA_12} , {80S_tRNA_eEF3_GTP_12} , {80S_13} , {80S_aa-tRNA_eEF1A_GTP_13} , {80S_aa-tRNA_13} , {80S_aa-tRNA_eEF2_GTP_13} , {80S_tRNA_13} , {80S_tRNA_eEF3_GTP_13} , {80S_14} , {80S_aa-tRNA_eEF1A_GTP_14} , {80S_aa-tRNA_14} , {80S_aa-tRNA_eEF2_GTP_14} , {80S_tRNA_14} , {80S_tRNA_eEF3_GTP_14} , {80S_15} , {80S_aa-tRNA_eEF1A_GTP_15} , {80S_aa-tRNA_15} , {80S_aa-tRNA_eEF2_GTP_15} , {80S_tRNA_15} , {80S_tRNA_eEF3_GTP_15} , {80S_16} , {80S_aa-tRNA_eEF1A_GTP_16} , {80S_aa-tRNA_16} , {80S_aa-tRNA_eEF2_GTP_16} , {80S_tRNA_16} , {80S_tRNA_eEF3_GTP_16} , {80S_17} , {80S_aa-tRNA_eEF1A_GTP_17} , {80S_aa-tRNA_17} , {80S_aa-tRNA_eEF2_GTP_17} , {80S_tRNA_17} , {80S_tRNA_eEF3_GTP_17} , {80S_18} , {80S_aa-tRNA_eEF1A_GTP_18} , {80S_aa-tRNA_18} , {80S_aa-tRNA_eEF2_GTP_18} , {80S_tRNA_18} , {80S_tRNA_eEF3_GTP_18} , {80S_19} , {80S_aa-tRNA_eEF1A_GTP_19} , {80S_aa-tRNA_19} , {80S_aa-tRNA_eEF2_GTP_19} , {80S_tRNA_19} , {80S_tRNA_eEF3_GTP_19} , {80S_20} , {80S_tRNA_20} , {80S_tRNA_eEF3_GTP_20}
 
 reaction28_5 [80S_tRNA_5] + [eEF3_GTP] → [80S_tRNA_eEF3_GTP_5];   {80S_tRNA_5} , {eEF3_GTP}
 
 reaction29_5 [80S_tRNA_eEF3_GTP_5] → [80S_5] + [eEF3_GDP] + [tRNA];   {80S_tRNA_eEF3_GTP_5}
 
 reaction22_6 [aa-tRNA_eEF1A_GTP] + [80S_6] ↔ [80S_aa-tRNA_eEF1A_GTP_6];   {aa-tRNA_eEF1A_GTP} , {80S_6} , {80S_aa-tRNA_eEF1A_GTP_6}
 
 reaction23_6 [80S_aa-tRNA_eEF1A_GTP_6] → [80S_aa-tRNA_6] + [eEF1A_GDP];   {80S_aa-tRNA_eEF1A_GTP_6}
 
 reaction25_6 [eEF2_GTP] + [80S_aa-tRNA_6] ↔ [80S_aa-tRNA_eEF2_GTP_6];   {eEF2_GTP} , {80S_aa-tRNA_6} , {80S_aa-tRNA_eEF2_GTP_6}
 
 reaction26_6 [80S_aa-tRNA_eEF2_GTP_6] → [80S_tRNA_7] + [eEF2_GDP];   {80S_aa-tRNA_eEF2_GTP_6}
 
 reaction28_6 [80S_tRNA_6] + [eEF3_GTP] → [80S_tRNA_eEF3_GTP_6];   {80S_tRNA_6} , {eEF3_GTP}
 
 reaction29_6 [80S_tRNA_eEF3_GTP_6] → [80S_6] + [eEF3_GDP] + [tRNA];   {80S_tRNA_eEF3_GTP_6}
 
 reaction22_7 [aa-tRNA_eEF1A_GTP] + [80S_7] ↔ [80S_aa-tRNA_eEF1A_GTP_7];   {aa-tRNA_eEF1A_GTP} , {80S_7} , {80S_aa-tRNA_eEF1A_GTP_7}
 
 reaction23_7 [80S_aa-tRNA_eEF1A_GTP_7] → [80S_aa-tRNA_7] + [eEF1A_GDP];   {80S_aa-tRNA_eEF1A_GTP_7}
 
 reaction25_7 [eEF2_GTP] + [80S_aa-tRNA_7] ↔ [80S_aa-tRNA_eEF2_GTP_7];   {eEF2_GTP} , {80S_aa-tRNA_7} , {80S_aa-tRNA_eEF2_GTP_7}
 
 reaction26_7 [80S_aa-tRNA_eEF2_GTP_7] → [80S_tRNA_8] + [eEF2_GDP];   {80S_aa-tRNA_eEF2_GTP_7}
 
 reaction28_7 [80S_tRNA_7] + [eEF3_GTP] → [80S_tRNA_eEF3_GTP_7];   {80S_tRNA_7} , {eEF3_GTP}
 
 reaction29_7 [80S_tRNA_eEF3_GTP_7] → [80S_7] + [eEF3_GDP] + [tRNA];   {80S_tRNA_eEF3_GTP_7}
 
 reaction22_8 [aa-tRNA_eEF1A_GTP] + [80S_8] ↔ [80S_aa-tRNA_eEF1A_GTP_8];   {aa-tRNA_eEF1A_GTP} , {80S_8} , {80S_aa-tRNA_eEF1A_GTP_8}
 
 reaction23_8 [80S_aa-tRNA_eEF1A_GTP_8] → [80S_aa-tRNA_8] + [eEF1A_GDP];   {80S_aa-tRNA_eEF1A_GTP_8}
 
 reaction25_8 [eEF2_GTP] + [80S_aa-tRNA_8] ↔ [80S_aa-tRNA_eEF2_GTP_8];   {eEF2_GTP} , {80S_aa-tRNA_8} , {80S_aa-tRNA_eEF2_GTP_8}
 
 reaction26_8 [80S_aa-tRNA_eEF2_GTP_8] → [80S_tRNA_9] + [eEF2_GDP];   {80S_aa-tRNA_eEF2_GTP_8}
 
 reaction28_8 [80S_tRNA_8] + [eEF3_GTP] → [80S_tRNA_eEF3_GTP_8];   {80S_tRNA_8} , {eEF3_GTP}
 
 reaction29_8 [80S_tRNA_eEF3_GTP_8] → [80S_8] + [eEF3_GDP] + [tRNA];   {80S_tRNA_eEF3_GTP_8}
 
 reaction22_9 [aa-tRNA_eEF1A_GTP] + [80S_9] ↔ [80S_aa-tRNA_eEF1A_GTP_9];   {aa-tRNA_eEF1A_GTP} , {80S_9} , {80S_aa-tRNA_eEF1A_GTP_9}
 
 reaction23_9 [80S_aa-tRNA_eEF1A_GTP_9] → [80S_aa-tRNA_9] + [eEF1A_GDP];   {80S_aa-tRNA_eEF1A_GTP_9}
 
 reaction25_9 [eEF2_GTP] + [80S_aa-tRNA_9] ↔ [80S_aa-tRNA_eEF2_GTP_9];   {eEF2_GTP} , {80S_aa-tRNA_9} , {80S_aa-tRNA_eEF2_GTP_9}
 
 reaction26_9 [80S_aa-tRNA_eEF2_GTP_9] → [80S_tRNA_10] + [eEF2_GDP];   {80S_aa-tRNA_eEF2_GTP_9}
 
 reaction28_9 [80S_tRNA_9] + [eEF3_GTP] → [80S_tRNA_eEF3_GTP_9];   {80S_tRNA_9} , {eEF3_GTP}
 
 reaction29_9 [80S_tRNA_eEF3_GTP_9] → [80S_9] + [eEF3_GDP] + [tRNA];   {80S_tRNA_eEF3_GTP_9}
 
 reaction22_10 [aa-tRNA_eEF1A_GTP] + [80S_10] ↔ [80S_aa-tRNA_eEF1A_GTP_10];   {aa-tRNA_eEF1A_GTP} , {80S_10} , {80S_aa-tRNA_eEF1A_GTP_10}
 
 reaction23_10 [80S_aa-tRNA_eEF1A_GTP_10] → [80S_aa-tRNA_10] + [eEF1A_GDP];   {80S_aa-tRNA_eEF1A_GTP_10}
 
 reaction25_10 [eEF2_GTP] + [80S_aa-tRNA_10] ↔ [80S_aa-tRNA_eEF2_GTP_10];   {eEF2_GTP} , {80S_aa-tRNA_10} , {80S_aa-tRNA_eEF2_GTP_10}
 
 reaction26_10 [80S_aa-tRNA_eEF2_GTP_10] → [80S_tRNA_11] + [eEF2_GDP];   {80S_aa-tRNA_eEF2_GTP_10}
 
 reaction28_10 [80S_tRNA_10] + [eEF3_GTP] → [80S_tRNA_eEF3_GTP_10];   {80S_tRNA_10} , {eEF3_GTP}
 
 reaction29_10 [80S_tRNA_eEF3_GTP_10] → [80S_10] + [eEF3_GDP] + [tRNA];   {80S_tRNA_eEF3_GTP_10}
 
 reaction22_11 [aa-tRNA_eEF1A_GTP] + [80S_11] ↔ [80S_aa-tRNA_eEF1A_GTP_11];   {aa-tRNA_eEF1A_GTP} , {80S_11} , {80S_aa-tRNA_eEF1A_GTP_11}
 
 reaction23_11 [80S_aa-tRNA_eEF1A_GTP_11] → [80S_aa-tRNA_11] + [eEF1A_GDP];   {80S_aa-tRNA_eEF1A_GTP_11}
 
 reaction25_11 [eEF2_GTP] + [80S_aa-tRNA_11] ↔ [80S_aa-tRNA_eEF2_GTP_11];   {eEF2_GTP} , {80S_aa-tRNA_11} , {80S_aa-tRNA_eEF2_GTP_11}
 
 reaction26_11 [80S_aa-tRNA_eEF2_GTP_11] → [80S_tRNA_12] + [eEF2_GDP];   {80S_aa-tRNA_eEF2_GTP_11}
 
 reaction28_11 [80S_tRNA_11] + [eEF3_GTP] → [80S_tRNA_eEF3_GTP_11];   {80S_tRNA_11} , {eEF3_GTP}
 
 reaction29_11 [80S_tRNA_eEF3_GTP_11] → [80S_11] + [eEF3_GDP] + [tRNA];   {80S_tRNA_eEF3_GTP_11}
 
 reaction22_12 [aa-tRNA_eEF1A_GTP] + [80S_12] ↔ [80S_aa-tRNA_eEF1A_GTP_12];   {aa-tRNA_eEF1A_GTP} , {80S_12} , {80S_aa-tRNA_eEF1A_GTP_12}
 
 reaction23_12 [80S_aa-tRNA_eEF1A_GTP_12] → [80S_aa-tRNA_12] + [eEF1A_GDP];   {80S_aa-tRNA_eEF1A_GTP_12}
 
 reaction25_12 [eEF2_GTP] + [80S_aa-tRNA_12] ↔ [80S_aa-tRNA_eEF2_GTP_12];   {eEF2_GTP} , {80S_aa-tRNA_12} , {80S_aa-tRNA_eEF2_GTP_12}
 
 reaction26_12 [80S_aa-tRNA_eEF2_GTP_12] → [80S_tRNA_13] + [eEF2_GDP];   {80S_aa-tRNA_eEF2_GTP_12}
 
 reaction28_12 [80S_tRNA_12] + [eEF3_GTP] → [80S_tRNA_eEF3_GTP_12];   {80S_tRNA_12} , {eEF3_GTP}
 
 reaction29_12 [80S_tRNA_eEF3_GTP_12] → [80S_12] + [eEF3_GDP] + [tRNA];   {80S_tRNA_eEF3_GTP_12}
 
 reaction22_13 [aa-tRNA_eEF1A_GTP] + [80S_13] ↔ [80S_aa-tRNA_eEF1A_GTP_13];   {aa-tRNA_eEF1A_GTP} , {80S_13} , {80S_aa-tRNA_eEF1A_GTP_13}
 
 reaction23_13 [80S_aa-tRNA_eEF1A_GTP_13] → [80S_aa-tRNA_13] + [eEF1A_GDP];   {80S_aa-tRNA_eEF1A_GTP_13}
 
 reaction25_13 [eEF2_GTP] + [80S_aa-tRNA_13] ↔ [80S_aa-tRNA_eEF2_GTP_13];   {eEF2_GTP} , {80S_aa-tRNA_13} , {80S_aa-tRNA_eEF2_GTP_13}
 
 reaction26_13 [80S_aa-tRNA_eEF2_GTP_13] → [80S_tRNA_14] + [eEF2_GDP];   {80S_aa-tRNA_eEF2_GTP_13}
 
 reaction28_13 [80S_tRNA_13] + [eEF3_GTP] → [80S_tRNA_eEF3_GTP_13];   {80S_tRNA_13} , {eEF3_GTP}
 
 reaction29_13 [80S_tRNA_eEF3_GTP_13] → [80S_13] + [eEF3_GDP] + [tRNA];   {80S_tRNA_eEF3_GTP_13}
 
 reaction22_14 [aa-tRNA_eEF1A_GTP] + [80S_14] ↔ [80S_aa-tRNA_eEF1A_GTP_14];   {aa-tRNA_eEF1A_GTP} , {80S_14} , {80S_aa-tRNA_eEF1A_GTP_14}
 
 reaction23_14 [80S_aa-tRNA_eEF1A_GTP_14] → [80S_aa-tRNA_14] + [eEF1A_GDP];   {80S_aa-tRNA_eEF1A_GTP_14}
 
 reaction25_14 [eEF2_GTP] + [80S_aa-tRNA_14] ↔ [80S_aa-tRNA_eEF2_GTP_14];   {eEF2_GTP} , {80S_aa-tRNA_14} , {80S_aa-tRNA_eEF2_GTP_14}
 
 reaction26_14 [80S_aa-tRNA_eEF2_GTP_14] → [80S_tRNA_15] + [eEF2_GDP];   {80S_aa-tRNA_eEF2_GTP_14}
 
 reaction28_14 [80S_tRNA_14] + [eEF3_GTP] → [80S_tRNA_eEF3_GTP_14];   {80S_tRNA_14} , {eEF3_GTP}
 
 reaction29_14 [80S_tRNA_eEF3_GTP_14] → [80S_14] + [eEF3_GDP] + [tRNA];   {80S_tRNA_eEF3_GTP_14}
 
 reaction22_15 [aa-tRNA_eEF1A_GTP] + [80S_15] ↔ [80S_aa-tRNA_eEF1A_GTP_15];   {aa-tRNA_eEF1A_GTP} , {80S_15} , {80S_aa-tRNA_eEF1A_GTP_15}
 
 reaction23_15 [80S_aa-tRNA_eEF1A_GTP_15] → [80S_aa-tRNA_15] + [eEF1A_GDP];   {80S_aa-tRNA_eEF1A_GTP_15}
 
 reaction25_15 [eEF2_GTP] + [80S_aa-tRNA_15] ↔ [80S_aa-tRNA_eEF2_GTP_15];   {eEF2_GTP} , {80S_aa-tRNA_15} , {80S_aa-tRNA_eEF2_GTP_15}
 
 reaction26_15 [80S_aa-tRNA_eEF2_GTP_15] → [80S_tRNA_16] + [eEF2_GDP];   {80S_aa-tRNA_eEF2_GTP_15}
 
 reaction28_15 [80S_tRNA_15] + [eEF3_GTP] → [80S_tRNA_eEF3_GTP_15];   {80S_tRNA_15} , {eEF3_GTP}
 
 reaction29_15 [80S_tRNA_eEF3_GTP_15] → [80S_15] + [eEF3_GDP] + [tRNA];   {80S_tRNA_eEF3_GTP_15}
 
 reaction22_16 [aa-tRNA_eEF1A_GTP] + [80S_16] ↔ [80S_aa-tRNA_eEF1A_GTP_16];   {aa-tRNA_eEF1A_GTP} , {80S_16} , {80S_aa-tRNA_eEF1A_GTP_16}
 
 reaction23_16 [80S_aa-tRNA_eEF1A_GTP_16] → [80S_aa-tRNA_16] + [eEF1A_GDP];   {80S_aa-tRNA_eEF1A_GTP_16}
 
 reaction25_16 [eEF2_GTP] + [80S_aa-tRNA_16] ↔ [80S_aa-tRNA_eEF2_GTP_16];   {eEF2_GTP} , {80S_aa-tRNA_16} , {80S_aa-tRNA_eEF2_GTP_16}
 
 reaction26_16 [80S_aa-tRNA_eEF2_GTP_16] → [80S_tRNA_17] + [eEF2_GDP];   {80S_aa-tRNA_eEF2_GTP_16}
 
 reaction28_16 [80S_tRNA_16] + [eEF3_GTP] → [80S_tRNA_eEF3_GTP_16];   {80S_tRNA_16} , {eEF3_GTP}
 
 reaction29_16 [80S_tRNA_eEF3_GTP_16] → [80S_16] + [eEF3_GDP] + [tRNA];   {80S_tRNA_eEF3_GTP_16}
 
 reaction22_17 [aa-tRNA_eEF1A_GTP] + [80S_17] ↔ [80S_aa-tRNA_eEF1A_GTP_17];   {aa-tRNA_eEF1A_GTP} , {80S_17} , {80S_aa-tRNA_eEF1A_GTP_17}
 
 reaction23_17 [80S_aa-tRNA_eEF1A_GTP_17] → [80S_aa-tRNA_17] + [eEF1A_GDP];   {80S_aa-tRNA_eEF1A_GTP_17}
 
 reaction25_17 [eEF2_GTP] + [80S_aa-tRNA_17] ↔ [80S_aa-tRNA_eEF2_GTP_17];   {eEF2_GTP} , {80S_aa-tRNA_17} , {80S_aa-tRNA_eEF2_GTP_17}
 
 reaction26_17 [80S_aa-tRNA_eEF2_GTP_17] → [80S_tRNA_18] + [eEF2_GDP];   {80S_aa-tRNA_eEF2_GTP_17}
 
 reaction28_17 [80S_tRNA_17] + [eEF3_GTP] → [80S_tRNA_eEF3_GTP_17];   {80S_tRNA_17} , {eEF3_GTP}
 
 reaction29_17 [80S_tRNA_eEF3_GTP_17] → [80S_17] + [eEF3_GDP] + [tRNA];   {80S_tRNA_eEF3_GTP_17}
 
 reaction22_18 [aa-tRNA_eEF1A_GTP] + [80S_18] ↔ [80S_aa-tRNA_eEF1A_GTP_18];   {aa-tRNA_eEF1A_GTP} , {80S_18} , {80S_aa-tRNA_eEF1A_GTP_18}
 
 reaction23_18 [80S_aa-tRNA_eEF1A_GTP_18] → [80S_aa-tRNA_18] + [eEF1A_GDP];   {80S_aa-tRNA_eEF1A_GTP_18}
 
 reaction25_18 [eEF2_GTP] + [80S_aa-tRNA_18] ↔ [80S_aa-tRNA_eEF2_GTP_18];   {eEF2_GTP} , {80S_aa-tRNA_18} , {80S_aa-tRNA_eEF2_GTP_18}
 
 reaction26_18 [80S_aa-tRNA_eEF2_GTP_18] → [80S_tRNA_19] + [eEF2_GDP];   {80S_aa-tRNA_eEF2_GTP_18}
 
 reaction28_18 [80S_tRNA_18] + [eEF3_GTP] → [80S_tRNA_eEF3_GTP_18];   {80S_tRNA_18} , {eEF3_GTP}
 
 reaction29_18 [80S_tRNA_eEF3_GTP_18] → [80S_18] + [eEF3_GDP] + [tRNA];   {80S_tRNA_eEF3_GTP_18}
 
 reaction22_19 [aa-tRNA_eEF1A_GTP] + [80S_19] ↔ [80S_aa-tRNA_eEF1A_GTP_19];   {aa-tRNA_eEF1A_GTP} , {80S_19} , {80S_aa-tRNA_eEF1A_GTP_19}
 
 reaction23_19 [80S_aa-tRNA_eEF1A_GTP_19] → [80S_aa-tRNA_19] + [eEF1A_GDP];   {80S_aa-tRNA_eEF1A_GTP_19}
 
 reaction25_19 [eEF2_GTP] + [80S_aa-tRNA_19] ↔ [80S_aa-tRNA_eEF2_GTP_19];   {eEF2_GTP} , {80S_aa-tRNA_19} , {80S_aa-tRNA_eEF2_GTP_19}
 
 reaction26_19 [80S_aa-tRNA_eEF2_GTP_19] → [80S_tRNA_20] + [eEF2_GDP];   {80S_aa-tRNA_eEF2_GTP_19}
 
 reaction28_19 [80S_tRNA_19] + [eEF3_GTP] → [80S_tRNA_eEF3_GTP_19];   {80S_tRNA_19} , {eEF3_GTP}
 
 reaction29_19 [80S_tRNA_eEF3_GTP_19] → [80S_19] + [eEF3_GDP] + [tRNA];   {80S_tRNA_eEF3_GTP_19}
 
 reaction28_20 [80S_tRNA_20] + [eEF3_GTP] → [80S_tRNA_eEF3_GTP_20];   {80S_tRNA_20} , {eEF3_GTP}
 
 reaction29_20 [80S_tRNA_eEF3_GTP_20] → [80S_20] + [eEF3_GDP] + [tRNA];   {80S_tRNA_eEF3_GTP_20}
 
 reaction30 [eRF3_GDP] ↔ [eRF3_GTP];   {eRF3_GDP} , {eRF3_GTP}
 
 reaction31 [eRF1] + [eRF3_GTP] ↔ [eRF1_eRF3_GTP];   {eRF1} , {eRF3_GTP} , {eRF1_eRF3_GTP}
 
 reaction32 [eRF1_eRF3_GTP] + [80S_20] → [80S_eRF1_eRF3_GTP];   {eRF1_eRF3_GTP} , {80S_20}
 
 reaction33 [80S_eRF1_eRF3_GTP] → [40S] + [60S] + [eRF1] + [eRF3_GDP];   {80S_eRF1_eRF3_GTP}
 
Rules (2)
 
 Assignment Rule (name: parameter_10) mRNA_bound = species_46+species_50+species_56+species_62+species_68+species_74+species_80+species_86+species_92+species_98+species_104+species_110+species_116+species_122+species_128+species_134+species_140+species_146+species_152+species_158+species_47+species_51+species_57+species_63+species_69+species_75+species_81+species_87+species_93+species_99+species_105+species_111+species_117+species_123+species_129+species_135+species_141+species_147+species_153+species_48+species_52+species_58+species_64+species_70+species_76+species_82+species_88+species_94+species_100+species_106+species_112+species_118+species_124+species_130+species_136+species_142+species_148+species_154+species_49+species_53+species_59+species_65+species_71+species_77+species_83+species_89+species_95+species_101+species_107+species_113+species_119+species_125+species_131+species_137+species_143+species_149+species_155+species_54+species_60+species_66+species_72+species_78+species_84+species_90+species_96+species_102+species_108+species_114+species_120+species_126+species_132+species_138+species_144+species_150+species_156+species_159+species_55+species_61+species_67+species_73+species_79+species_85+species_91+species_97+species_103+species_109+species_115+species_121+species_127+species_133+species_139+species_145+species_151+species_157+species_160+species_165
 
 Assignment Rule (name: parameter_11) mRNA_free = parameter_1-parameter_10
 
Functions (3)
 
 Initiation with blocking [1] [1] lambda(k, substrate1, substrate2, mRNA_tot, A1, B1, C1, D1, A2, B2, C2, D2, E2, F2, A3, B3, C3, D3, E3, F3, A4, B4, C4, D4, E4, F4, A5, B5, C5, D5, E5, F5, A6, B6, C6, D6, E6, F6, A7, B7, C7, D7, E7, F7, A8, B8, C8, D8, E8, F8, A9, B9, C9, D9, E9, F9, A10, B10, C10, D10, E10, F10, A11, B11, C11, D11, E11, F11, A12, B12, C12, D12, E12, F12, A13, B13, C13, D13, E13, F13, A14, B14, C14, D14, E14, F14, A15, B15, C15, D15, E15, F15, k*substrate1*substrate2*(mRNA_tot-(A1+B1+C1+D1+A2+B2+C2+D2+E2+F2+A3+B3+C3+D3+E3+F3+A4+B4+C4+D4+E4+F4+A5+B5+C5+D5+E5+F5+A6+B6+C6+D6+E6+F6+A7+B7+C7+D7+E7+F7+A8+B8+C8+D8+E8+F8+A9+B9+C9+D9+E9+F9+A10+B10+C10+D10+E10+F10+A11+B11+C11+D11+E11+F11+A12+B12+C12+D12+E12+F12+A13+B13+C13+D13+E13+F13+A14+B14+C14+D14+E14+F14+A15+B15+C15+D15+E15+F15)))
 
 Translocation with blocking (interior) [1] [1] lambda(k, substrate, mRNA_tot, A1, B1, C1, D1, E1, F1, A2, B2, C2, D2, E2, F2, A3, B3, C3, D3, E3, F3, A4, B4, C4, D4, E4, F4, A5, B5, C5, D5, E5, F5, A6, B6, C6, D6, E6, F6, A7, B7, C7, D7, E7, F7, A8, B8, C8, D8, E8, F8, A9, B9, C9, D9, E9, F9, A10, B10, C10, D10, E10, F10, A11, B11, C11, D11, E11, F11, A12, B12, C12, D12, E12, F12, A13, B13, C13, D13, E13, F13, A14, B14, C14, D14, E14, F14, A15, B15, C15, D15, E15, F15, k*substrate*(mRNA_tot-(A1+B1+C1+D1+E1+F1+A2+B2+C2+D2+E2+F2+A3+B3+C3+D3+E3+F3+A4+B4+C4+D4+E4+F4+A5+B5+C5+D5+E5+F5+A6+B6+C6+D6+E6+F6+A7+B7+C7+D7+E7+F7+A8+B8+C8+D8+E8+F8+A9+B9+C9+D9+E9+F9+A10+B10+C10+D10+E10+F10+A11+B11+C11+D11+E11+F11+A12+B12+C12+D12+E12+F12+A13+B13+C13+D13+E13+F13+A14+B14+C14+D14+E14+F14+A15+B15+C15+D15+E15+F15))/(mRNA_tot-(A1+B1+C1+D1+E1+F1+A2+B2+C2+D2+E2+F2+A3+B3+C3+D3+E3+F3+A4+B4+C4+D4+E4+F4+A5+B5+C5+D5+E5+F5+A6+B6+C6+D6+E6+F6+A7+B7+C7+D7+E7+F7+A8+B8+C8+D8+E8+F8+A9+B9+C9+D9+E9+F9+A10+B10+C10+D10+E10+F10+A11+B11+C11+D11+E11+F11+A12+B12+C12+D12+E12+F12+A13+B13+C13+D13+E13+F13+A14+B14+C14+D14+E14+F14)))
 
 Translocation with blocking (for codon number (length(mRNA)-length(Ribosome)) [1] [1] lambda(k, substrate, mRNA_tot, A1, B1, C1, D1, E1, F1, A2, B2, C2, D2, E2, F2, A3, B3, C3, D3, E3, F3, A4, B4, C4, D4, E4, F4, A5, B5, C5, D5, E5, F5, A6, B6, C6, D6, E6, F6, A7, B7, C7, D7, E7, F7, A8, B8, C8, D8, E8, F8, A9, B9, C9, D9, E9, F9, A10, B10, C10, D10, E10, F10, A11, B11, C11, D11, E11, F11, A12, B12, C12, D12, E12, F12, A13, B13, C13, D13, E13, F13, A14, B14, C14, D14, E14, F14, A15, E15, F15, k*substrate*(mRNA_tot-(A1+B1+C1+D1+E1+F1+A2+B2+C2+D2+E2+F2+A3+B3+C3+D3+E3+F3+A4+B4+C4+D4+E4+F4+A5+B5+C5+D5+E5+F5+A6+B6+C6+D6+E6+F6+A7+B7+C7+D7+E7+F7+A8+B8+C8+D8+E8+F8+A9+B9+C9+D9+E9+F9+A10+B10+C10+D10+E10+F10+A11+B11+C11+D11+E11+F11+A12+B12+C12+D12+E12+F12+A13+B13+C13+D13+E13+F13+A14+B14+C14+D14+E14+F14+A15+E15+F15))/(mRNA_tot-(A1+B1+C1+D1+E1+F1+A2+B2+C2+D2+E2+F2+A3+B3+C3+D3+E3+F3+A4+B4+C4+D4+E4+F4+A5+B5+C5+D5+E5+F5+A6+B6+C6+D6+E6+F6+A7+B7+C7+D7+E7+F7+A8+B8+C8+D8+E8+F8+A9+B9+C9+D9+E9+F9+A10+B10+C10+D10+E10+F10+A11+B11+C11+D11+E11+F11+A12+B12+C12+D12+E12+F12+A13+B13+C13+D13+E13+F13+A14+B14+C14+D14+E14+F14)))
 
 cytoplasm Spatial dimensions: 3.0  Compartment size: 4.0E-14
 
 eIF2_GDP
Compartment: cytoplasm
Initial concentration: 1.17510804502861E-6
 
 eIF2B
Compartment: cytoplasm
Initial concentration: 2.26917415591732E-7
 
 eIF2_GDP_eIF2B
Compartment: cytoplasm
Initial concentration: 0.0
 
 eIF2_GTP
Compartment: cytoplasm
Initial concentration: 0.0
 
 Met-tRNA
Compartment: cytoplasm
Initial concentration: 2.59334189247694E-5
Constant
 
 eIF2_GTP_Met-tRNA
Compartment: cytoplasm
Initial concentration: 0.0
 
 eIF3
Compartment: cytoplasm
Initial concentration: 9.31982242608899E-7
 
 eIF5
Compartment: cytoplasm
Initial concentration: 8.10419341399042E-7
 
 eIF3_eIF5
Compartment: cytoplasm
Initial concentration: 0.0
 
 eIF3_eIF5_eIF2_GTP_Met-tRNA
Compartment: cytoplasm
Initial concentration: 0.0
 
 eIF1
Compartment: cytoplasm
Initial concentration: 1.53979674865818E-6
 
 eIF1_eIF3_eIF5_eIF2_GTP_Met-tRNA
Compartment: cytoplasm
Initial concentration: 0.0
 
 40S
Compartment: cytoplasm
Initial concentration: 7.69898374329091E-6
 
 eIF1A
Compartment: cytoplasm
Initial concentration: 2.30969512298727E-6
 
 40S_eIF1A
Compartment: cytoplasm
Initial concentration: 0.0
 
 43S
Compartment: cytoplasm
Initial concentration: 0.0
 
 eIF4E
Compartment: cytoplasm
Initial concentration: 3.24167736559617E-6
 
 eIF4G
Compartment: cytoplasm
Initial concentration: 8.91461275538947E-7
 
 eIF4E_eIF4G
Compartment: cytoplasm
Initial concentration: 0.0
 
 mRNA_cap
Compartment: cytoplasm
Initial concentration: 7.16464328895E-7
 
 Pab1
Compartment: cytoplasm
Initial concentration: 1.78292255107789E-6
 
 mRNA_Pab1
Compartment: cytoplasm
Initial concentration: 0.0
 
 eIF4E_eIF4G_mRNA_Pab1
Compartment: cytoplasm
Initial concentration: 0.0
 
 eIF4A
Compartment: cytoplasm
Initial concentration: 9.72503209678849E-6
 
 eIF4B
Compartment: cytoplasm
Initial concentration: 1.13458707795866E-6
 
 eIF4A_eIF4B
Compartment: cytoplasm
Initial concentration: 0.0
 
 eIF4E_eIF4G_mRNA_Pab1_eIF4A_eIF4B
Compartment: cytoplasm
Initial concentration: 0.0
 
 48S
Compartment: cytoplasm
Initial concentration: 0.0
 
 Ded1
Compartment: cytoplasm
Initial concentration: 1.13458707795866E-6
 
 48S_Ded1
Compartment: cytoplasm
Initial concentration: 0.0
 
 eIF5B_GDP
Compartment: cytoplasm
Initial concentration: 1.9450064193577E-7
 
 eIF5B_GTP
Compartment: cytoplasm
Initial concentration: 0.0
 
 48S_Ded1_eIF5B_GTP
Compartment: cytoplasm
Initial concentration: 0.0
 
 60S
Compartment: cytoplasm
Initial concentration: 7.6989833745501E-6
 
 eEF1A_GDP
Compartment: cytoplasm
Initial concentration: 4.05209670699521E-5
 
 eEF1B
Compartment: cytoplasm
Initial concentration: 3.20115639852622E-6
 
 eEF1A_GDP_eEF1B
Compartment: cytoplasm
Initial concentration: 0.0
 
 eEF1A_GTP
Compartment: cytoplasm
Initial concentration: 0.0
 
 aa-tRNA
Compartment: cytoplasm
Initial concentration: 5.18668391964828E-4
Constant
 
 aa-tRNA_eEF1A_GTP
Compartment: cytoplasm
Initial concentration: 0.0
 
 eEF2_GDP
Compartment: cytoplasm
Initial concentration: 5.6729353897933E-6
 
 eEF2_GTP
Compartment: cytoplasm
Initial concentration: 0.0
 
 eEF3_GDP
Compartment: cytoplasm
Initial concentration: 4.45730637769471E-6
 
 eEF3_GTP
Compartment: cytoplasm
Initial concentration: 0.0
 
 tRNA
Compartment: cytoplasm
Initial concentration: 2.59333683546025E-4
Constant
 
 80S_1
Compartment: cytoplasm
Initial concentration: 0.0
 
 80S_aa-tRNA_eEF1A_GTP_1
Compartment: cytoplasm
Initial concentration: 0.0
 
 80S_aa-tRNA_1
Compartment: cytoplasm
Initial concentration: 0.0
 
 80S_aa-tRNA_eEF2_GTP_1
Compartment: cytoplasm
Initial concentration: 0.0
 
 80S_2
Compartment: cytoplasm
Initial concentration: 0.0
 
 80S_aa-tRNA_eEF1A_GTP_2
Compartment: cytoplasm
Initial concentration: 0.0
 
 80S_aa-tRNA_2
Compartment: cytoplasm
Initial concentration: 0.0
 
 80S_aa-tRNA_eEF2_GTP_2
Compartment: cytoplasm
Initial concentration: 0.0
 
 80S_tRNA_2
Compartment: cytoplasm
Initial concentration: 0.0
 
 80S_tRNA_eEF3_GTP_2
Compartment: cytoplasm
Initial concentration: 0.0
 
 80S_3
Compartment: cytoplasm
Initial concentration: 0.0
 
 80S_aa-tRNA_eEF1A_GTP_3
Compartment: cytoplasm
Initial concentration: 0.0
 
 80S_aa-tRNA_3
Compartment: cytoplasm
Initial concentration: 0.0
 
 80S_aa-tRNA_eEF2_GTP_3
Compartment: cytoplasm
Initial concentration: 0.0
 
 80S_tRNA_3
Compartment: cytoplasm
Initial concentration: 0.0
 
 80S_tRNA_eEF3_GTP_3
Compartment: cytoplasm
Initial concentration: 0.0
 
 80S_4
Compartment: cytoplasm
Initial concentration: 0.0
 
 80S_aa-tRNA_eEF1A_GTP_4
Compartment: cytoplasm
Initial concentration: 0.0
 
 80S_aa-tRNA_4
Compartment: cytoplasm
Initial concentration: 0.0
 
 80S_aa-tRNA_eEF2_GTP_4
Compartment: cytoplasm
Initial concentration: 0.0
 
 80S_tRNA_4
Compartment: cytoplasm
Initial concentration: 0.0
 
 80S_tRNA_eEF3_GTP_4
Compartment: cytoplasm
Initial concentration: 0.0
 
 80S_5
Compartment: cytoplasm
Initial concentration: 0.0
 
 80S_aa-tRNA_eEF1A_GTP_5
Compartment: cytoplasm
Initial concentration: 0.0
 
 80S_aa-tRNA_5
Compartment: cytoplasm
Initial concentration: 0.0
 
 80S_aa-tRNA_eEF2_GTP_5
Compartment: cytoplasm
Initial concentration: 0.0
 
 80S_tRNA_5
Compartment: cytoplasm
Initial concentration: 0.0
 
 80S_tRNA_eEF3_GTP_5
Compartment: cytoplasm
Initial concentration: 0.0
 
 80S_6
Compartment: cytoplasm
Initial concentration: 0.0
 
 80S_aa-tRNA_eEF1A_GTP_6
Compartment: cytoplasm
Initial concentration: 0.0
 
 80S_aa-tRNA_6
Compartment: cytoplasm
Initial concentration: 0.0
 
 80S_aa-tRNA_eEF2_GTP_6
Compartment: cytoplasm
Initial concentration: 0.0
 
 80S_tRNA_6
Compartment: cytoplasm
Initial concentration: 0.0
 
 80S_tRNA_eEF3_GTP_6
Compartment: cytoplasm
Initial concentration: 0.0
 
 80S_7
Compartment: cytoplasm
Initial concentration: 0.0
 
 80S_aa-tRNA_eEF1A_GTP_7
Compartment: cytoplasm
Initial concentration: 0.0
 
 80S_aa-tRNA_7
Compartment: cytoplasm
Initial concentration: 0.0
 
 80S_aa-tRNA_eEF2_GTP_7
Compartment: cytoplasm
Initial concentration: 0.0
 
 80S_tRNA_7
Compartment: cytoplasm
Initial concentration: 0.0
 
 80S_tRNA_eEF3_GTP_7
Compartment: cytoplasm
Initial concentration: 0.0
 
 80S_8
Compartment: cytoplasm
Initial concentration: 0.0
 
 80S_aa-tRNA_eEF1A_GTP_8
Compartment: cytoplasm
Initial concentration: 0.0
 
 80S_aa-tRNA_8
Compartment: cytoplasm
Initial concentration: 0.0
 
 80S_aa-tRNA_eEF2_GTP_8
Compartment: cytoplasm
Initial concentration: 0.0
 
 80S_tRNA_8
Compartment: cytoplasm
Initial concentration: 0.0
 
 80S_tRNA_eEF3_GTP_8
Compartment: cytoplasm
Initial concentration: 0.0
 
 80S_9
Compartment: cytoplasm
Initial concentration: 0.0
 
 80S_aa-tRNA_eEF1A_GTP_9
Compartment: cytoplasm
Initial concentration: 0.0
 
 80S_aa-tRNA_9
Compartment: cytoplasm
Initial concentration: 0.0
 
 80S_aa-tRNA_eEF2_GTP_9
Compartment: cytoplasm
Initial concentration: 0.0
 
 80S_tRNA_9
Compartment: cytoplasm
Initial concentration: 0.0
 
 80S_tRNA_eEF3_GTP_9
Compartment: cytoplasm
Initial concentration: 0.0
 
 80S_10
Compartment: cytoplasm
Initial concentration: 0.0
 
 80S_aa-tRNA_eEF1A_GTP_10
Compartment: cytoplasm
Initial concentration: 0.0
 
 80S_aa-tRNA_10
Compartment: cytoplasm
Initial concentration: 0.0
 
 80S_aa-tRNA_eEF2_GTP_10
Compartment: cytoplasm
Initial concentration: 0.0
 
 80S_tRNA_10
Compartment: cytoplasm
Initial concentration: 0.0
 
 80S_tRNA_eEF3_GTP_10
Compartment: cytoplasm
Initial concentration: 0.0
 
 80S_11
Compartment: cytoplasm
Initial concentration: 0.0
 
 80S_aa-tRNA_eEF1A_GTP_11
Compartment: cytoplasm
Initial concentration: 0.0
 
 80S_aa-tRNA_11
Compartment: cytoplasm
Initial concentration: 0.0
 
 80S_aa-tRNA_eEF2_GTP_11
Compartment: cytoplasm
Initial concentration: 0.0
 
 80S_tRNA_11
Compartment: cytoplasm
Initial concentration: 0.0
 
 80S_tRNA_eEF3_GTP_11
Compartment: cytoplasm
Initial concentration: 0.0
 
 80S_12
Compartment: cytoplasm
Initial concentration: 0.0
 
 80S_aa-tRNA_eEF1A_GTP_12
Compartment: cytoplasm
Initial concentration: 0.0
 
 80S_aa-tRNA_12
Compartment: cytoplasm
Initial concentration: 0.0
 
 80S_aa-tRNA_eEF2_GTP_12
Compartment: cytoplasm
Initial concentration: 0.0
 
 80S_tRNA_12
Compartment: cytoplasm
Initial concentration: 0.0
 
 80S_tRNA_eEF3_GTP_12
Compartment: cytoplasm
Initial concentration: 0.0
 
 80S_13
Compartment: cytoplasm
Initial concentration: 0.0
 
 80S_aa-tRNA_eEF1A_GTP_13
Compartment: cytoplasm
Initial concentration: 0.0
 
 80S_aa-tRNA_13
Compartment: cytoplasm
Initial concentration: 0.0
 
 80S_aa-tRNA_eEF2_GTP_13
Compartment: cytoplasm
Initial concentration: 0.0
 
 80S_tRNA_13
Compartment: cytoplasm
Initial concentration: 0.0
 
 80S_tRNA_eEF3_GTP_13
Compartment: cytoplasm
Initial concentration: 0.0
 
 80S_14
Compartment: cytoplasm
Initial concentration: 0.0
 
 80S_aa-tRNA_eEF1A_GTP_14
Compartment: cytoplasm
Initial concentration: 0.0
 
 80S_aa-tRNA_14
Compartment: cytoplasm
Initial concentration: 0.0
 
 80S_aa-tRNA_eEF2_GTP_14
Compartment: cytoplasm
Initial concentration: 0.0
 
 80S_tRNA_14
Compartment: cytoplasm
Initial concentration: 0.0
 
 80S_tRNA_eEF3_GTP_14
Compartment: cytoplasm
Initial concentration: 0.0
 
 80S_15
Compartment: cytoplasm
Initial concentration: 0.0
 
 80S_aa-tRNA_eEF1A_GTP_15
Compartment: cytoplasm
Initial concentration: 0.0
 
 80S_aa-tRNA_15
Compartment: cytoplasm
Initial concentration: 0.0
 
 80S_aa-tRNA_eEF2_GTP_15
Compartment: cytoplasm
Initial concentration: 0.0
 
 80S_tRNA_15
Compartment: cytoplasm
Initial concentration: 0.0
 
 80S_tRNA_eEF3_GTP_15
Compartment: cytoplasm
Initial concentration: 0.0
 
 80S_16
Compartment: cytoplasm
Initial concentration: 0.0
 
 80S_aa-tRNA_eEF1A_GTP_16
Compartment: cytoplasm
Initial concentration: 0.0
 
 80S_aa-tRNA_16
Compartment: cytoplasm
Initial concentration: 0.0
 
 80S_aa-tRNA_eEF2_GTP_16
Compartment: cytoplasm
Initial concentration: 0.0
 
 80S_tRNA_16
Compartment: cytoplasm
Initial concentration: 0.0
 
 80S_tRNA_eEF3_GTP_16
Compartment: cytoplasm
Initial concentration: 0.0
 
 80S_17
Compartment: cytoplasm
Initial concentration: 0.0
 
 80S_aa-tRNA_eEF1A_GTP_17
Compartment: cytoplasm
Initial concentration: 0.0
 
 80S_aa-tRNA_17
Compartment: cytoplasm
Initial concentration: 0.0
 
 80S_aa-tRNA_eEF2_GTP_17
Compartment: cytoplasm
Initial concentration: 0.0
 
 80S_tRNA_17
Compartment: cytoplasm
Initial concentration: 0.0
 
 80S_tRNA_eEF3_GTP_17
Compartment: cytoplasm
Initial concentration: 0.0
 
 80S_18
Compartment: cytoplasm
Initial concentration: 0.0
 
 80S_aa-tRNA_eEF1A_GTP_18
Compartment: cytoplasm
Initial concentration: 0.0
 
 80S_aa-tRNA_18
Compartment: cytoplasm
Initial concentration: 0.0
 
 80S_aa-tRNA_eEF2_GTP_18
Compartment: cytoplasm
Initial concentration: 0.0
 
 80S_tRNA_18
Compartment: cytoplasm
Initial concentration: 0.0
 
 80S_tRNA_eEF3_GTP_18
Compartment: cytoplasm
Initial concentration: 0.0
 
 80S_19
Compartment: cytoplasm
Initial concentration: 0.0
 
 80S_aa-tRNA_eEF1A_GTP_19
Compartment: cytoplasm
Initial concentration: 0.0
 
 80S_aa-tRNA_19
Compartment: cytoplasm
Initial concentration: 0.0
 
 80S_aa-tRNA_eEF2_GTP_19
Compartment: cytoplasm
Initial concentration: 0.0
 
 80S_tRNA_19
Compartment: cytoplasm
Initial concentration: 0.0
 
 80S_tRNA_eEF3_GTP_19
Compartment: cytoplasm
Initial concentration: 0.0
 
 80S_20
Compartment: cytoplasm
Initial concentration: 0.0
 
 80S_tRNA_20
Compartment: cytoplasm
Initial concentration: 0.0
 
 80S_tRNA_eEF3_GTP_20
Compartment: cytoplasm
Initial concentration: 0.0
 
 eRF3_GDP
Compartment: cytoplasm
Initial concentration: 5.6729353897933E-7
 
 eRF3_GTP
Compartment: cytoplasm
Initial concentration: 0.0
 
 eRF1
Compartment: cytoplasm
Initial concentration: 8.91461275538947E-7
 
 eRF1_eRF3_GTP
Compartment: cytoplasm
Initial concentration: 0.0
 
 80S_eRF1_eRF3_GTP
Compartment: cytoplasm
Initial concentration: 0.0
 
 eIF4A_eIF4E_eIF4G_mRNA_Pab1
Compartment: cytoplasm
Initial concentration: 0.0
 
Global Parameters (12)
 
 mRNA_tot
Value: 7.16464328895E-7
Constant
 
 k22f
Value: 8.10035535716195E9
Constant
 
 k22b
Value: 0.284007213965168
Constant
 
 k23f
Value: 28324.3562938545
Constant
 
 k25f
Value: 3.10377169466493E9
Constant
 
 k25b
Value: 0.00322599
Constant
 
 k26f
Value: 2306950.0
Constant
 
 k28f
Value: 2.24052E9
Constant
 
 k29f
Value: 72911.6740026381
Constant
 
  mRNA_bound  
 
  mRNA_free
Value: 7.16464328895E-7
 
 mRNA_tot_Molar
Value: 2.86585731558E-20
Constant
 
reaction1 (2)
 
   k1
Value: 1.96096E7
Constant
 
   k2
Value: 34.8025
Constant
 
reaction2 (2)
 
   k1
Value: 533.26
Constant
 
   k2
Value: 3.97698
Constant
 
reaction3 (2)
 
   k1
Value: 104798.0
Constant
 
   k2
Value: 6.32998
Constant
 
reaction4 (2)
 
   k1
Value: 2.00245E7
Constant
 
   k2
Value: 0.406929
Constant
 
reaction5 (2)
 
   k1
Value: 1.60265E8
Constant
 
   k2
Value: 57.1854
Constant
 
reaction6 (2)
 
   k1
Value: 3865650.0
Constant
 
   k2
Value: 31.1969
Constant
 
reaction7 (1)
 
   k1
Value: 814356.0
Constant
 
reaction8 (1)
 
   k1
Value: 1.97254E7
Constant
 
reaction9 (2)
 
   k1
Value: 4.33274E7
Constant
 
   k2
Value: 1977.92
Constant
 
reaction10 (2)
 
   k1
Value: 5026500.0
Constant
 
   k2
Value: 0.00774034
Constant
 
reaction11 (2)
 
   k1
Value: 5.79912E7
Constant
 
   k2
Value: 2.70026
Constant
 
reaction12 (2)
 
   k1
Value: 307831.0
Constant
 
   k2
Value: 2.38184
Constant
 
reaction13 (2)
 
   k1
Value: 3.5208E14
Constant
 
   k2
Value: 0.785013
Constant
 
reaction14 (1)
 
   k1
Value: 1.06204E9
Constant
 
reaction15 (2)
 
   k1
Value: 8.7134E10
Constant
 
   k2
Value: 1.2395
Constant
 
reaction16 (2)
 
   k1
Value: 304.768
Constant
 
   k2
Value: 45.4082
Constant
 
reaction17 (2)
 
   k1
Value: 5.61005E8
Constant
 
   k2
Value: 47.8215
Constant
 
reaction18 (1)
 
   k
Value: 1.3072E13
Constant
 
reaction19 (2)
 
   k1
Value: 1170250.0
Constant
 
   k2
Value: 14.2912
Constant
 
reaction20 (2)
 
   k1
Value: 13.295
Constant
 
   k2
Value: 4.33541E7
Constant
 
reaction21 (2)
 
   k1
Value: 39591.9
Constant
 
   k2
Value: 8.80234E-6
Constant
 
reaction24 (2)
 
   k1
Value: 1.80542
Constant
 
   k2
Value: 1.29513
Constant
 
reaction27 (2)
 
   k1
Value: 93.5995
Constant
 
   k2
Value: 43714.4
Constant
 
reaction30 (2)
 
   k1
Value: 14200.8
Constant
 
   k2
Value: 18032.6
Constant
 
reaction31 (2)
 
   k1
Value: 1.6E9
Constant
 
   k2
Value: 35378.1
Constant
 
reaction32 (1)
 
   k1
Value: 1.53068E8
Constant
 
reaction33 (1)
 
   k1
Value: 3.5491E9
Constant
 
Representative curation result(s)
Representative curation result(s) of BIOMD0000000457

Curator's comment: (updated: 27 Feb 2013 18:12:07 GMT)

Figure 2g (k26 versus free ribosome) is reproduced here, in concentrations.
The data were obtained by simulating the model using Copasi v4.8 (Build 35). The plot was generated using Gnuplot.

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