P-CAGE-25485 - P-CAGE-25485
Total RNA was isolated according to Logemann et al. (1987) and the using RNeasy mini kit (QIAGEN, Hilden, Germany). RNA was quantified by use of a Nanodrop ND-1000 UV-Vis Spectrophotometer (Nanodrop Technology, Wilminton, DE) and assessed using an Agilent 2100 Bioanalyzer (Agilent Technology, Palo Alto, CA). 1 ug of total RNA from each sample was amplified and aminoallyl-labeled using MessageAmpTM II aRNA kit (Ambion), which contains ArrayScript enzyme mix, and 5-(3-aminoallyl)-2'deoxyuridine-5'-triphosphate (aa-dUTP, Ambion) and following the manufacturer's instructions.
Amplification, Extracted product