P-GSE40677-4 - P-GSE40677-4
nucleic acid extraction protocol
Mice were euthanized by carbon dioxide inhalation followed by cervical dislocation, and hearts were excised postmortem. Hearts were homogenized in Trizol (Invitrogen) and total RNA was extracted following the manufacturer’s protocol. RNA samples were DNase-treated using the DNA-free Kit (Ambion) and cleaned up using the RNeasy Kit (Qiagen) following manufacturer’s protocols. RNA concentrations and quality were confirmed on a Nanodrop ND-1000 spectrophotometer and Agilent Bio-analyzer.