P-GSE48179-3 - P-GSE48179-3
sample treatment protocol
Name:Benzene 1h - environmental treatment - abiotic stress,benzene:time 1hour . 2 months old cabbages are exposed to 3ppm of atmospheric benzene in a 300 liters hermetic chamber. Exposures were conducted over periods of 1h, 3h and 24h. We made biological replicates of exposures of 1h and 3h. As controls, non-exposed plants are located next the exposure chamber for each experiment. At the end of the exposure, pieces of leaves from 3 different plants were pooled and immediatly flash-frozen with liquid nitrogen and stored at -80°C until RNA extraction.